CA2112130A1 - Screening Assay for the Detection of DNA-Binding Molecules - Google Patents

Screening Assay for the Detection of DNA-Binding Molecules

Info

Publication number
CA2112130A1
CA2112130A1 CA2112130A CA2112130A CA2112130A1 CA 2112130 A1 CA2112130 A1 CA 2112130A1 CA 2112130 A CA2112130 A CA 2112130A CA 2112130 A CA2112130 A CA 2112130A CA 2112130 A1 CA2112130 A1 CA 2112130A1
Authority
CA
Canada
Prior art keywords
dna
sequence
binding
test
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2112130A
Other languages
French (fr)
Other versions
CA2112130C (en
Inventor
Cynthia A. Edwards
Charles R. Cantor
Beth M. Andrews
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genelabs Technologies Inc
Original Assignee
Cynthia A. Edwards
Charles R. Cantor
Beth M. Andrews
Genelabs Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cynthia A. Edwards, Charles R. Cantor, Beth M. Andrews, Genelabs Technologies, Inc. filed Critical Cynthia A. Edwards
Publication of CA2112130A1 publication Critical patent/CA2112130A1/en
Application granted granted Critical
Publication of CA2112130C publication Critical patent/CA2112130C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

Abstract

The present invention defines an assay use-ful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA
test sequences. The assay is also useful for deter-mining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. The assay is a competition assay in which binding of a test mole-cule to a DNA test sequence changes the binding characteristics of a DNA-binding protein to its binding sequence. When such a test molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the ratio between unbound DNA and DNA:protein complexes. The assay is versatile in that any test sequence can be tested by placing the test sequence adjacent to a defined protein binding DNA screening sequence.
CA002112130A 1991-06-27 1992-06-26 Screening assay for the detection of dna-binding molecules Expired - Fee Related CA2112130C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72361891A 1991-06-27 1991-06-27
US07/723,618 1991-06-27

Publications (2)

Publication Number Publication Date
CA2112130A1 true CA2112130A1 (en) 1993-01-07
CA2112130C CA2112130C (en) 1996-08-06

Family

ID=24907008

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002112130A Expired - Fee Related CA2112130C (en) 1991-06-27 1992-06-26 Screening assay for the detection of dna-binding molecules

Country Status (12)

Country Link
US (5) US5693463A (en)
EP (2) EP0823486A3 (en)
JP (1) JP3169610B2 (en)
KR (1) KR100235575B1 (en)
AT (1) ATE165397T1 (en)
AU (1) AU655839B2 (en)
CA (1) CA2112130C (en)
DE (1) DE69225227T2 (en)
DK (1) DK0593618T3 (en)
ES (1) ES2117050T3 (en)
HK (1) HK1010058A1 (en)
WO (1) WO1993000446A1 (en)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5849478A (en) * 1986-08-14 1998-12-15 Cashman; Daniel P. Blocked-polymerase polynucleotide immunoassay method and kit
US5723289A (en) * 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US5578444A (en) * 1991-06-27 1996-11-26 Genelabs Technologies, Inc. Sequence-directed DNA-binding molecules compositions and methods
DE69225227T2 (en) * 1991-06-27 1998-10-29 Genelabs Tech Inc SCREENING TEST FOR DETECTING DNA-BINDING MOLECULES
US5726014A (en) * 1991-06-27 1998-03-10 Genelabs Technologies, Inc. Screening assay for the detection of DNA-binding molecules
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US8153602B1 (en) 1991-11-19 2012-04-10 Isis Pharmaceuticals, Inc. Composition and methods for the pulmonary delivery of nucleic acids
US5616461A (en) * 1992-05-14 1997-04-01 Dana-Farber Cancer Institute Assay for antiviral activity using complex of herpesvirus origin of replication and cellular protein
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US6824976B1 (en) * 1993-04-02 2004-11-30 Rigel Pharmaceuticals, Inc. Method for selective inactivation of viral replication
US20030099945A1 (en) * 1994-09-20 2003-05-29 Invenux, Inc. Parallel selex
US20030104361A1 (en) * 1997-09-29 2003-06-05 Susan Weininger Method of detection of nucleic acids with a specific sequence composition
US5871902A (en) * 1994-12-09 1999-02-16 The Gene Pool, Inc. Sequence-specific detection of nucleic acid hybrids using a DNA-binding molecule or assembly capable of discriminating perfect hybrids from non-perfect hybrids
US7803529B1 (en) 1995-04-11 2010-09-28 Sequenom, Inc. Solid phase sequencing of biopolymers
US20060063193A1 (en) * 1995-04-11 2006-03-23 Dong-Jing Fu Solid phase sequencing of double-stranded nucleic acids
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
DE69627333T2 (en) 1995-06-26 2004-02-12 PerSeptive Biosystems, Inc., Framingham AUTOMATED, CONTINUOUS, MULTI-DIMENSIONAL, HIGH-SPEED, MOLECULAR SELECTION AND ANALYSIS
WO1997009342A1 (en) 1995-09-08 1997-03-13 Scriptgen Pharmaceuticals, Inc. Screen for compounds with affinity for rna
JP2000501068A (en) * 1995-09-12 2000-02-02 フィテラ シンビオン エイピーエス Actinomycin D analog
US6514746B1 (en) 1995-09-15 2003-02-04 Essential Therapeutics, Inc. Staphylococcus aureus histidine protein kinase essential genes
US6037123A (en) 1995-09-15 2000-03-14 Microcide Pharmaceuticals, Inc. Methods of screening for compounds active on Staphylococcus aureus target genes
US5989827A (en) * 1995-11-14 1999-11-23 Abbott Laboratories Use of nuclear magnetic resonance to design ligands to target biomolecules
US5891643A (en) * 1995-11-14 1999-04-06 Abbott Laboratories Use of nuclear magnetic resonance to design ligands to target biomolecules
US5698401A (en) * 1995-11-14 1997-12-16 Abbott Laboratories Use of nuclear magnetic resonance to identify ligands to target biomolecules
CA2190304A1 (en) * 1995-12-15 1997-06-16 Elazar Rabbani Property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses
US5747256A (en) * 1995-12-19 1998-05-05 Beckman Instruments, Inc. Homogeneous DNA probe titration assay
US6602657B1 (en) 1995-12-28 2003-08-05 Tropix, Inc. Multiple reporter gene assay
WO1997024457A1 (en) 1995-12-29 1997-07-10 Guilford Pharmaceuticals Inc. Method and kit for detection of multiple protein interactions
US6143901A (en) * 1996-07-31 2000-11-07 Genesoft, Inc. Complex formation between dsDNA and pyrrole imidazole polyamides
US6635417B1 (en) 1996-07-31 2003-10-21 California Institute Of Technology Complex formation between DSDNA and oligomer of cyclic heterocycles
WO1998045284A1 (en) 1996-02-26 1998-10-15 California Institute Of Technology Stereochemical control of the dna binding affinity, sequence specificity, and orientation-preference of chiral hairpin polyamides in the minor groove
US6506906B1 (en) 1996-02-26 2003-01-14 California Institute Of Technology Preparation and use of bifunctional molecules having DNA sequence binding specificity
US6090947A (en) 1996-02-26 2000-07-18 California Institute Of Technology Method for the synthesis of pyrrole and imidazole carboxamides on a solid support
US6555692B1 (en) 1996-02-26 2003-04-29 California Institute Of Technology Preparation and use of bifunctional molecules having DNA sequence binding specificity
EP0968186A1 (en) 1996-02-26 2000-01-05 California Institute Of Technology Improved polyamides for binding in the minor groove of double stranded dna
US5955590A (en) * 1996-07-15 1999-09-21 Worcester Foundation For Biomedical Research Conjugates of minor groove DNA binders with antisense oligonucleotides
US5998140A (en) 1996-07-31 1999-12-07 The Scripps Research Institute Complex formation between dsDNA and oligomer of cyclic heterocycles
WO1998039484A1 (en) 1997-03-05 1998-09-11 Scriptgen Pharmaceuticals, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US5939261A (en) * 1997-06-24 1999-08-17 Sarnoff Corporation Method for capturing a nucleic acid
US5972650A (en) * 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
JP2002510319A (en) 1997-07-01 2002-04-02 アイシス・ファーマシューティカルス・インコーポレーテッド Compositions and methods for delivery of oligonucleotides through the gastrointestinal tract
AU8574898A (en) 1997-07-14 1999-02-08 Valentis, Inc. Method for the identification of synthetic cell- or tissue-specific transcriptional regulatory regions
US6326489B1 (en) 1997-08-05 2001-12-04 Howard Hughes Medical Institute Surface-bound, bimolecular, double-stranded DNA arrays
JP2001516058A (en) * 1997-09-12 2001-09-25 ジェネラブス テクノロジーズ，インコーポレイテッド Methods and compositions for dsRNA / dsRNA binding proteins
JP2001519538A (en) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ Replica amplification of nucleic acid arrays
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6548021B1 (en) 1997-10-10 2003-04-15 President And Fellows Of Harvard College Surface-bound, double-stranded DNA protein arrays
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
WO1999042621A2 (en) * 1998-02-21 1999-08-26 Tm Technologies, Inc. Methods for identifying or characterising a site based on the thermodynamic properties of nucleic acids
EP0945507A1 (en) * 1998-03-27 1999-09-29 Boehringer Mannheim Gmbh Tumor specific expression controll region and its use
US7321828B2 (en) * 1998-04-13 2008-01-22 Isis Pharmaceuticals, Inc. System of components for preparing oligonucleotides
US20040186071A1 (en) * 1998-04-13 2004-09-23 Bennett C. Frank Antisense modulation of CD40 expression
WO1999060167A1 (en) * 1998-05-21 1999-11-25 Isis Pharmaceuticals, Inc. Compositions and methods for topical delivery of oligonucleotides
EP1469009A2 (en) * 1998-05-21 2004-10-20 Isis Parmaceuticals, Inc. Compositions and methods for non-parenteral delivery of oligonucleotides
US6723517B1 (en) 1998-06-02 2004-04-20 Minerva Biotechnologies Corporation Use of self-assembled monolayers to probe the structure of a target molecule
US6100035A (en) 1998-07-14 2000-08-08 Cistem Molecular Corporation Method of identifying cis acting nucleic acid elements
US6203990B1 (en) 1998-11-06 2001-03-20 Mitokor Method and system for pattern analysis, such as for analyzing oligonucleotide primer extension assay products
DE69834208T2 (en) * 1998-12-10 2007-01-25 Daniel P. El Dorado Hills Cashman BLOCKED POLYMERASE POLYNUCLEOTIDE IMMUNOASSAY AND KIT
US7189506B1 (en) 1999-03-03 2007-03-13 Genelabs Technologies, Inc. DNA binding compound-mediated molecular switch system
US6824987B1 (en) * 1999-05-11 2004-11-30 President And Fellows Of Harvard College Small molecule printing
US7932213B2 (en) * 1999-05-11 2011-04-26 President And Fellows Of Harvard College Small molecule printing
DE19926216A1 (en) * 1999-06-09 2001-02-22 Metallgesellschaft Ag Process for producing barium sulfate, barium sulfate and use of barium sulfate
US6764858B2 (en) 1999-09-29 2004-07-20 Pharmacia & Upjohn Company Methods for creating a compound library
US6677160B1 (en) 1999-09-29 2004-01-13 Pharmacia & Upjohn Company Methods for creating a compound library and identifying lead chemical templates and ligands for target molecules
WO2001025417A2 (en) * 1999-10-01 2001-04-12 Sangamo Biosciences, Inc. Dna library and its use in methods of selecting and designing polypeptides
US6613517B2 (en) 2000-01-15 2003-09-02 Genelabs Technologies, Inc. Nucleic acid binding assay and selection method
US6500436B2 (en) 2000-01-19 2002-12-31 Allergan, Inc. Clostridial toxin derivatives and methods for treating pain
US6641820B1 (en) 2000-01-19 2003-11-04 Allergan, Inc. Clostridial toxin derivatives and methods to treat pain
US7138127B1 (en) 2000-01-19 2006-11-21 Allergan, Inc. Clostridial toxin derivatives and methods for treating pain
AU2001251502A1 (en) * 2000-04-17 2001-10-30 Pharmacia And Upjohn Company Nuclear magnetic resonance methods for identifying sites in papillomavirus e2 protein
CA2407695C (en) * 2000-04-28 2015-03-31 Sangamo Biosciences, Inc. Methods for binding an exogenous molecule to cellular chromatin
US6660229B2 (en) 2000-06-13 2003-12-09 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
EP1313868B1 (en) * 2000-08-30 2006-07-19 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
CN1258593C (en) * 2000-11-07 2006-06-07 北卡罗莱纳州立大学 Putrescine-N-methyl transferase promoter
AU2002220255A1 (en) * 2000-12-05 2002-06-18 The Penn State Research Foundation A monoclonal antibody-based diagnostic assay for gamma fibrinogen
US20040241650A1 (en) * 2001-01-19 2004-12-02 Evans Glen A Computer-directed assembly of a polynucleotide encoding a target polypeptide
US7595147B2 (en) * 2001-04-25 2009-09-29 Wisconsin Alumni Research Foundation Methods to identify agents that bind the flap-tip helix of RNA polymerase
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
US6821737B2 (en) 2001-06-08 2004-11-23 Panomics, Inc. Method for screening for drug candidates for modulating transcription factor activity
US6924113B2 (en) 2001-06-08 2005-08-02 Panomics, Inc. Method and kit for isolating DNA probes that bind to activated transcription factors
US7981842B2 (en) 2001-06-08 2011-07-19 Panomics, Inc. Method for detecting transcription factor-protein interactions
MXPA03011385A (en) * 2001-06-08 2005-03-07 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco.
US20040214166A1 (en) * 2001-06-08 2004-10-28 Xianqiang Li Method for identifying a disease state based on a detected mixture of activated transcription factors
US6696256B1 (en) 2001-06-08 2004-02-24 Pandmics, Inc. Method, array and kit for detecting activated transcription factors by hybridization array
US7115232B2 (en) * 2001-07-13 2006-10-03 Hudson Gordon S Fluorescence validation microplate and method of use
US7179907B2 (en) * 2001-12-18 2007-02-20 Bruce Eaton Antibiotic compounds
US7001718B2 (en) * 2001-12-20 2006-02-21 Massachusetts Institute Of Technology Method of inhibiting pathogenicity of infectious agents
US20030143547A1 (en) * 2002-01-24 2003-07-31 Xianqiang Li Method for identifying multiple activated transcription factors
EP1499188A4 (en) * 2002-04-09 2007-11-14 Vector Tobacco Ltd Tobacco having reduced nicotine and nitrosamines
AU2003260786B2 (en) * 2002-09-06 2008-03-13 Isogenica Limited In vitro peptide expression libraray
WO2004071453A2 (en) * 2003-02-13 2004-08-26 Isis Pharmaceuticals, Inc. Compositions and methods for treatment of pouchitis
US7004001B2 (en) * 2003-02-26 2006-02-28 Formcek Cleveland, Inc. Roll forming apparatus for forming sheet material into multiple shapes
US7399853B2 (en) * 2003-04-28 2008-07-15 Isis Pharmaceuticals Modulation of glucagon receptor expression
US7750142B2 (en) * 2003-04-28 2010-07-06 Isis Pharmaceuticals, Inc. Modulation of glucagon receptor expression
JP3711988B2 (en) * 2003-05-12 2005-11-02 株式会社日立製作所 Fine particle array analysis system, fine particle array kit, and chemical analysis method
WO2005018552A2 (en) * 2003-08-12 2005-03-03 University Of Utah Research Foundation Heparin binding proteins: sensors for heparin detection
US20050142581A1 (en) * 2003-09-04 2005-06-30 Griffey Richard H. Microrna as ligands and target molecules
CA2635929A1 (en) * 2006-01-03 2008-01-31 David W. Barnes Small molecule printing
JP5437790B2 (en) 2006-04-28 2014-03-12 チルドレンズ ホスピタル メディカル センター Composition comprising a fusion protein or polypeptide derived from prosaposin for application to a transmembrane drug delivery system
US8639445B2 (en) * 2007-07-23 2014-01-28 Microsoft Corporation Identification of related residues in biomolecular sequences by multiple sequence alignment and phylogenetic analysis
CN101903035A (en) * 2007-10-23 2010-12-01 阿勒根公司 Methods of treating chronic neurogenic inflammation using modified clostridial toxins
US8916531B2 (en) 2007-11-20 2014-12-23 Isis Pharmaceuticals, Inc. Modulation of CD40 expression
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
CN103339509A (en) 2011-01-28 2013-10-02 佰欧迪塞克斯公司 Predictive test for selection of metastatic breast cancer patients for hormonal and combination therapy
EP2758533B1 (en) 2011-09-20 2018-04-11 Ionis Pharmaceuticals, Inc. Antisense modulation of gcgr expression
DK2790736T3 (en) 2011-12-12 2018-05-07 Oncoimmunin Inc In vivo delivery of oligonucleotides

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4270924A (en) * 1979-04-10 1981-06-02 Crooke Stanley T Diagnostic method for detecting cancer
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4942227A (en) * 1982-01-11 1990-07-17 California Institute Of Technology Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid, their preparation and use to cleave DNA
US4665184A (en) * 1983-10-12 1987-05-12 California Institute Of Technology Bifunctional molecules having a DNA intercalator or DNA groove binder linked to ethylene diamine tetraacetic acid
DE3789883T2 (en) * 1986-01-09 1994-10-06 Massachusetts Inst Technology NUCLEAR FACTORS ASSOCIATED WITH REGULATION OF TRANSCRIPTION.
US5071773A (en) * 1986-10-24 1991-12-10 The Salk Institute For Biological Studies Hormone receptor-related bioassays
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
WO1992005286A1 (en) * 1990-09-24 1992-04-02 Roger Brent Screening assays
AU1469292A (en) * 1991-01-18 1992-08-27 Oncogene Science, Inc. Methods of transcriptionally modulating expression of growth factor genes and growth factor receptor genes
DE69225227T2 (en) * 1991-06-27 1998-10-29 Genelabs Tech Inc SCREENING TEST FOR DETECTING DNA-BINDING MOLECULES
US5726014A (en) * 1991-06-27 1998-03-10 Genelabs Technologies, Inc. Screening assay for the detection of DNA-binding molecules

Also Published As

Publication number Publication date
JP3169610B2 (en) 2001-05-28
KR100235575B1 (en) 1999-12-15
US5306619A (en) 1994-04-26
CA2112130C (en) 1996-08-06
EP0593618B1 (en) 1998-04-22
EP0593618A1 (en) 1994-04-27
AU655839B2 (en) 1995-01-12
ES2117050T3 (en) 1998-08-01
US5693463A (en) 1997-12-02
EP0823486A2 (en) 1998-02-11
US5738990A (en) 1998-04-14
JPH07500491A (en) 1995-01-19
US5716780A (en) 1998-02-10
DE69225227D1 (en) 1998-05-28
WO1993000446A1 (en) 1993-01-07
DE69225227T2 (en) 1998-10-29
AU2297892A (en) 1993-01-25
HK1010058A1 (en) 1999-06-11
US5744131A (en) 1998-04-28
DK0593618T3 (en) 1998-11-23
ATE165397T1 (en) 1998-05-15
EP0823486A3 (en) 2004-02-11

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