CA2098306A1 - Discontinuous and nonsequential polymeric gel systems for separation of macromolecules - Google Patents

Discontinuous and nonsequential polymeric gel systems for separation of macromolecules

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Publication number
CA2098306A1
CA2098306A1 CA002098306A CA2098306A CA2098306A1 CA 2098306 A1 CA2098306 A1 CA 2098306A1 CA 002098306 A CA002098306 A CA 002098306A CA 2098306 A CA2098306 A CA 2098306A CA 2098306 A1 CA2098306 A1 CA 2098306A1
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Prior art keywords
gel
layers
gels
layer
discrete
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA002098306A
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French (fr)
Inventor
Jan J. Opplt
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Individual
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Individual
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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D57/00Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44773Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

This invention relates to the formation and use of a variable, multilayer system of discrete, discontinuous and nonsequential molecular sieves, comprising of agarose-acrylic gels of distinct composition. Each of the individual gel-layers of the system is designed according to partition and/or retardation coefficients for specific retention and therefore selective separation of selected molecules of different molecular sizes. The unique multilayer composite acrylic gel system of this invention makes it possible to separate micro- and macromolecules which differ in molecular weights by millions of daltons. So isolated any physically defined molecular groups are superiorly suitable for further chemical, physico-chemical or biological characterization.

Description

WO92/10277 ; ` PCT/US91/09~
f 209~30G

Title: DISCONTINUOUS AND NONSEQUENTIAL POLYMERIC GEL
SYSTE~S FOR SEPARATION OF MACROMOLECULES

Cro~ Re~e~enc0 to ~el~ted Applica~io~s This application is a continuation-in-part of an application entitled "Discontinuous and Nonsequential Polyacrylamide Gel 10Systems For Separation Of Macromolecules", Serial No. 07/627,054~
filing date December 13, 1990. Applicant incorporates the disclosure and claims of said application Serial No. 07/~27l054 by reference herein in their entirety and claims the benefit of said application for all purposes pursuant to 37 C.F.R. 1O78.

lSBa¢kground of the I~ve~tion 1. Field of the Invention This invention relates generally to methods and a system for the separation and resolution of molecular components, including synthetic, organir or biologic particles, from a mixture of such components. More particularly, the invention pertains to a multilayer molecular filtration system comprised of discontinuous and nonse~uential, gelous, semifluid or fluid~
chromatographic or electrophoretic mediae placed on or inside or around different and presently known and used mechanical vectors, facilita'cing a movement of polar particles with various molecular characteristics and related methods thereof.
2. Descr ption of Related Art The use of sieving gels for the separation of organical and/or biological particles is w ll-known and used in the SUB~ITUTE 8HEET

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el~ctrophoresis art from mid-fifties of this century. However, the presently known methods, based on sieving e~fects demonstrat-ed throughout the years, have serious disadvantages. The most apparent is the inability to separate, satisfactorily, the high and very high molecular particles, e.g., molecular weighks greater than 650,000 daltons.
The sieving gels which are most often used ~or analytical and scientific purposes include the following cellulose acetate, starch gels, agar-gels, agarose-gels, gels of agarose deri~atives,polyacrylamide gels, gradient polyacrylamide gels of di~ferent gradients (from 2% to 27% o~ PAA and 4% to 30%
of P~A), pore gradient polyacrylamide gels, mixture of, or combinations of polyacrylamide gels and different derivatives of agarose, as well as any of the above gels with addition of protein-complexes-forming variants (SDS), urea, or emulators (TRITONS), or glass adhesives, etc.
All the above-mentioned gels and combinations thereof are known to be used only in continuous forms (e.g., nongradient mediae), or continuous gradients (e.g., gradient mediae). For example, the most often employed gelous mediae are the agaros~-qels. Although gels of different concentrations of purified agarose are utilized for specific purposes (e.g., l~ , 2%, 2~%, etc.), all are known as "continuous" gels. In comparisonl the "gradient" gels comprise gels (usually polyacrylamidic~ of increasing concentrations of acrylamide monomer, as shown by the formula where T equals the percent of monomers concentration:

g ACRY~ + g BIS
T = --------------------- x l00 l00 ml gel SUBSI ITUl~ SHEET

~092/l0277 ~ q 6` ` ~ PCT/US91/Og~8 . .

These gels are known to be used in different ranges of their gradients (for instance from 3-7%, or 2-5%, or 2-16%, or 2-27%), but these gradients are known to be always sequentially assorted (e.g~, in 3-4-5-6-7%, or 2-3-4-5%, or in 2-3-4-5-6-7~8-9-10-11 12-13-14-15-16% fashion, etc.).
It is noted that the literature discloses only few devices of similar arrangement of two electrophoretic gels from which two gels designated (A,B) were called "discontinuous", and one designated (C) was described as a gel with a "stepped gradi-ent". Howaver, none of the above arrangements suggest a variable multilayer system of distinct electrophoretic gels, forming specific molecular sieves characterized by retention gradients for elected groups of polar biomolecules.
The above designated devices (A, B and C) only comprise two (2) layers of gels wherein the first layer serves primarily as a mechanical, and not as an analytical means (e.g., delivery of the biological sample to an analytical gel (A), or to clean the analyzed biological sample from interPering components (B), or to unspecified improvement of desired analytical procedures (C)). In addition, certain ones, called "technical" gels are composed of co~ponents which are substantially smaller , than are the main, analytical gels (A, B, C).
U.S. Patent No. 4,306,956 to De Castro describes the first of the two-layer systems designated (A~. This patent is directed to the use of thymol blue, phenol red, o-cresol red, orange G, m-cresol purple and mixtures, as a tracking dye in an SDS-PAGE electrophoresis process. The patent describes system designated (A) as follows - "OPten in discontinuous sodium-SUBSl~ITUTE SHEIET

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dodecyl sulfate polyacrylamide electrophoretic gels (U.S. Patent No. 3,384,564), two separately polymerized layers of polyacryl-amide, designated as 'a stacking gel' and 'a separating gel', are prepared." (Col. 1, lines 40-42). The large pore size of "the stacking gels" allows the biological sample to concentrate into narrow starting zone, necessary for good resolution in the following gel, with no change in characteristics, until it encounters a discontinuity in entering "the separating gel9l, either in the nature of the supporting medium, i.e., pore size, or in the buffer, e.g., pH. This change, based on en~ering into second gel, produces the separation of the different macro-molecular species into discrete bands, but it does not relate to the present invention.
The second of the two-layer systems (B) was also called "discontinuous" by S. Moulin, J.C. Fruchart, P. Dewailly and G. Sezille - Electrophorese des lipoproteines seriques sur plaque de g~l d'acrylamide-agarose, en gradient discontinu d'acrylamideO
Clinca Chimica Acta, 91 (1979):159-163. The authors used two different concentrations (2% and 3%) o~ acrylamide and a constant concentration of agaro~e (0.7%). The biological samples are placed in the first gel (2~) in order to retain macromolecular component~ (e.g., chylomicrons), which hinder a good electr-ophoretic separation of other lower molecular components of diagnostic interest. Howeverj the entire analytical separation takes place on a homogeneous second gel (3%), without any further interference of the ~irst layer which clearly serves only as a technical preserver of the unwanted material and therefore does not rel2te to the present invention.

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, U.S. Patent No. 4,189,370 to Boschetti descrlbes the third of these two-layer systems (designated (C~) which was referred to as a "stepped gradient". The patent is directed to a process for preparing gels stratified upon a film, comprising two steps: first, disposing upon said film gel, being formed from a first monomer concentration and disposing upnn said first film a second gel, so as to form "a discontinuous gradient"
between said first gel and seoond gel; these layers may be spaced from polyester film and the second layer may also be disposed on said first layer. The author failed to demonætrate how the separation of biomacromolecules is enhanced by said invented separation line (between layers). However, the so claimed, but not proven "separation" may be often artificial, or otherwise incorrect, or may bP related to wrongly dPfined fractions, etcO
The ideas of Boschetti (1980) and de Castro (1981) are basically identical, although their inventions differ in concen-trations of recommended gels for electrophoresis of serum lipoproteins and/or proteins - de Castro's so called "stacking gel" has lower T% than his "separating gradient gel" offered in a wide variety of gradually increasing compositions from 0.5% to 30% T; preferred range is from about 3-27% T. Similarly, Boschetti's first gel has lower concentratio~s of said bi-functional allylic or acrylic compound e.g., about 7.5%, while the nongradient second gel has higher concentrations, e.g., about 10~17%, although concentrations of the monomers in the said gels differ not significantly, e.g., 2.5% and 2.0-~, respectively.
However, none of the above-described methodical modifications of separating gels make possible to retain and so SUBSl m~TE 5HEET .,~

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separate the analyzed biomolecules according to their specific retention gradients into their standard classes and subclasses.
This is described in detail harein, under EXAMPLE 1 and graph~
ically presented in FIG. II wherein a pattern obtained with de Castro's method (classified as GRAD) can be compared with the method of this inventor (classified as DISC).
U.S. ~atent No. 3,384,564 to L. Ornstein is direoted to "a simultaneous separation and concentration of charged particles which may be either separately recovered or altern-atively the resulting continuous thin zones of charged particles used thereafter as the starting zones with appropriate pH and/or pore size change for high resolution and further separation of the components in brief runs." (Column 1, lines 10-19) The patent states that "with a synthetic polymer like polyacrylamidel because the average pore size of a gel depends on the concen-tration of polymer (e.g., a 30% gel produces about a 20 ~ pore and 7~% about 50), the pore size can be adjusted to the range of dimensions of the molecules to be separated" and gives the dimensions in ~ and the free mobilities of a few plasma proteins at pH 8.6 (mobility unit = l05cm2/volt-sec.). (Column 1, line 70 - column 7, line 5) L. ornstein stated his basic working idea as follows:
"It would appear that a 7~% aqueous polyacrylamide should exhibit extreme frictional resistance to the migration of fibrinogen, '- 25 lipoprotein (and perhaps the a2 macroglobulin and .gamma~
~`~ globulin), and that the other proteins should be able to pass ,~ through, though with substantially moxe difficulty than in an 7 . aqueous system. Thus differences in molecular size as well as SUBSnTUTE SHEEr , ~

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charge can be utilized in electrophoretic separation of various components in a gel or solution of linear polymer."
L. Ornstein's claims of an electrophoresis, using three layers, although in fact only two real layers are described by i" 5 him - a large pore.specimen layer (it is not presently considered '~ as a separate layer), large pore gel spacer layer (later called "stackinq gel") and a ~mall pore gel layer ~or further separatiDn (= on 7.5% PAAG) of analyzed particles in a vertical cylindrical j container, equipped with buffer reservoirs and electrodes, ;- 10 fraction elutor and sample collector. Thus, Ornstein does not relate to the present invention.
~ Commercially, the combination of a "stacking and :~ separating gel" has been utilized according to de Castro by Miles .$ Laboratories, ElXhart, Indiana, under the trademark "LipoPhor-j. 15 Gels". They were primarily used for HDL-cholesterol assaying by D. Roche, V. Atger, N.T. Le Quang, A. Girard, and O. G . Edindjian .~ in the work "Polyacryla~ide Gel Electrophoresis in Quantification of ~igh-Density hipoprotein Cholesterol", Clinical ChemistrY, 31/11 (1985), 1893-1985. The autAors found good correlations between total HDL-cholesterol analyses and corresponding ultracentrifugal values, but never attempted to achieve the separation of HD-lipoproteins according to their molecul~r sizesO
Their work does not interfere with this invention.
More sophisticatedly were removed from the biologic mixture of serum lipoproteins the VLDL (and ehylomicrons) by the accumulation at the 5 mm thick 2%, 5% PAAG boundary, while LDL
just entered the 5% PAAG. The separation between the above ; lipoprotein groups of VLDL and LDL thus obtained "was variable, i .
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and not sufficiently clear for analytical purposes." "The effect of the 2% and 5~ gel phases was therefore to retard the LDL and VLDL over some distance of the gel system without compressing these into a thin concentrated band at a gel boundary", according 5 to the authors ~.B. Ubbing, ~.J. Serfontein and L.S. deVilliers in an article "The Direct Quantification in Whole Serum of HD~
Subfractions", ~;liniça Chimica Acta, lZ5 (1982), 165-175.
These authors achieved the above-described "stacking effect" of ~DL for some kind of separation of the HDL into HD~
. 10 and HD~ subfractions, roughly corresponding to the ultra-,. centrifugally isolated ~DL classes at the density 1.125 g/ml (HDLi) and 1.21 g/ml (HD~, using an additional 3 mm thick -`i. polyacrylamide gel (PAAG), dimensions 160 x 120 mm, consisting , of a 13% gel of classical composition at pH = 8.9.
.~ .

i 15 8ummar~ of the I~v~ntion :~ There is provided by the present invention a multilayered molecular filtration system for electrophoresis of high and very high molecular substances, such as certain proteins, lipo-proteins, glycoproteins, nucleotides, etc. The filtration system ~`~. 20 of the invention comprises at least f iYe layers arranged in a ~: successive, discrete, discontinuous and nonsequential fashion according to partition and/or retardation coefficients for every group of assorted macromolecules to be analyzed. This makes uniquely possible the separation of molecules accordiny to molecular weights which may differ by millions of daltons.
In one embodiment, the novel molecular sieves are employed ,i in the form of polyacrylamide gels of different densities which :i~ SUBSI~lTlJTE 8HEFr .,, - - ~ ,., , , :

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,/ 1 _ 9 _ ' are arranged into discrete discontinuous and nonsequential $ multilayered molecular filtration systems.
There is further disclosed by the present invention a thin ~' substrate for electrophoretic separations of high-molecular substances, consisting of nonsequential, discrete, discontinuous and multitudinous organization of polyacrylamide gel-layers with different concentration of monomers. They may contain constant concentrations of "poring" polysaccharides, such as ayaroses and/or its derivatives, or sepharoses, dextrans, etc. In 10 optional layers, immunofixative or other material binding biological molecules can be usPd.
It will be readily appreciated that the multilayered filtra-tion system for electrophoresis, microelectrophoresis or chromatographic separation in accordance with the present invention advantageously is preparable in any of practically used sizes and forms (e.g., plates, columns, capillaries, etc.), and maybe utilized for mass production.

- ~rief Description of the Dr~winqs ~` FIG. I illustrates examples of compositions of different multitudinous discrete sequential gel systems and discrete nonsequential multitudinous gelous molecular sieves with specific ~- separation coefficients;
FIG. II shows a comparison between analytical potentials of i discrete polyacrylamide gels with sequentially increasing concen~
t.rations ~ersus discrete, nonsequential and multitudinous poly-acrylamide-agarose gels, forming sieves ~ith specific partition coefficients for analyzed macromolecules; and .~' .
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.~ FIG. III is a ~chematic representation o~ molecular electro-phoresis based on use of novel systems of discrete nonsequential multitudinous gelous molecular sieves with specific separation ~: coefficients.
., , Detailed DescriPt~on The multilayered molecular ~iltration systems in accordance . with the invention comprise a series of at least about 5 discontinuous and nonsequential molecular sieves or separating gel-layers which are e~fecti~e to separate particles according :; 10 to a broad range of molecular weights when arranged in a regular ~: or irregular, i.e., sequential or nonsequential, successions manner such that selected molecules or molecular groups are retained by the specific "binding" or sieving layers of the . filtration system. The largest ~olecules are engaged by the ~` 15 first sieving layer, while all other molecules of less size pass :~ into the next layer. The next sieve selectively keeps groups of ; the next large molecules, but releases the r~st of the molecules of lesser size until separation of the molecular groups is completed. OptiDnally, tha filtration system may contain a i~ 20 number of non-sieving or "nonbinding" layers which function to .;,: increase the resolution of the separation in certain cases.
The particles or molecules which may be separated are of various origin (synthetic or occurring in the nature) with unre-stricted significanc~, e.g., in chemistry, pharmacology, ~; 25 biochemis~ry, pathophysiology, plant or animal physiology, or in medicine.

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The separating gel layers may b~ prepared from all known media lncluding, but not limited to, cellulose acetate, starch 'f,' gels, agar-gels, agaro~e-gels, gels of agarose derivatives, polyacrylamide gels, gradient polyacrylamide gels of different gradients (e.g., from 2~ to 27~ of PAA and 4~ to 30% of PAA), pore gradient polyacrylamide gels, mixture of, or combinations of polyacrylamide gels and different derivatives of ag~rose or other polysaccharides, immunofixative or biospecific sorbants ,as - well as any of the above gels with addition of protein-complexes-forming variants (SDS), urea, or emulators (TRITONS), or glass adhesives, etc. The gels may be solid, semifluid or fluido f! This invention is most advantageous in separations and/or y~ determinations of molecular weights Of unknown particles, or those mixtures of particles with very broad molecular sizes and ; 15 weights or mixtures of very small as well as very large par-ticles.
I The object of the discrete system of layers is to filter~upO
.~ .
or otherwise separate macromolecular mixtures according to their molecular sizes in such a manner that each of the layers excluded (or retains, or delays) groups of molecules of higher order and ~ releases all other molecules of lower order, e.g~, of smaller ; molecular sizes, until the groups of the smallest molecular size ~ are retained at the end of the mediae while none of the molecules ! are lost.
1 25 An optimal composition of discrete layers may be selected ;f';' or developed using adequate molecular standards and/or molecular ~ markers covering the entire molecular scale of separated mixtures `~ or complexes.

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209~306 The above speclfic conditions are ~est defined by the partition coefficient KD and by the retardation coefficient K~
(designed by K.A. Ferguson : Metabolism, 13 985~ 1964)o ~.
;~
The retardation coefficient of Ferquson is yiven byo K~ = (R ~ r) C
where C = ( 0 ~ 4 3 4~
where 1' is the length of the fiber chain per unit volume, R the i' radius of the partitioning molecul~, and r the radius of the gel fiber.
~: 10 According to the theories of gel filtration, KR should depend only on the gel structure and the size of the partitioning protein.
i The partition coefflcient, KD~ f the protein between the . gel and solution, as obtained from gel filtration experiments, , 15 can be related to KR/ by the following relationship :
.,~ log KD = ~ R~T
. where T is a gel concentration.
If the values Of KR O~' analyzed macromolecules are plotted ,.s, ~ against the size of radii (R) of these partitioning molecules (in 'f 20 A) at a standard pH, a linear relationship is obtained (according :~ to experience o~' S. Ghosh, M.K. Basu and J.S. Schweppe ,~ ~ Analytical Biochemistry, 50 : 592-601, 19723.
In the following text of this invention, there will be ~: described all molecular sieves constructed in conformity with the .~ 25 above definitions f KD and RR~ called as discrete, di~continuous :. .
and nonsequential molecular sieves with specific partition and/or retardation coefficients calculated and/or experimentally proven . SlJBSl~mJTE 8~1EET
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for a sp~cific fraction or class or subclass of analyzed complex of macromolecules.
These systems are particularly efficient in the form of discrete layers of identical length or widely varying length depending on the composition, structure, network, shape or gradation of these changes in their quality or composition. In ' general, there is no limit to the length of specific layersO In one embodiment, the layers are at least about 0.2mm. In another embodiment, the layers are from about 0.5~m to about lOcm.
As noted above, there are at least about 5 separating gel-~ layers or sieving layers in the system. In one embodiment, the 7 ,~ system comprises ~rom about 5 to about 30 layers. Depending upon the macromolecules to be analyzed, t'ae system will comprise , ' e.g., about 6-30 and, more often, about 18 gel-layers (poly~
, 15 peptides and/or serum proteins) with variable concentrations of copolymers in the range of from 2-30~ by weight to about 2-27%
by weight; about 6-18 separating gel-layers and, advantageously 12 layers (serum lipoproteins) with variable concentrations of copolymers in the range of 2-20% by weight to about 2-17~ by `~ 20 weight; about 6-16 separating gel-layers, advantageously 8 layers .,.~ . .
~; (serum glyco proteins) with variable concentrations of copolymexs in the range of 3-27% by weight; about 5-20 separating gel-layers ( oligonucleotides and nucleic acids and/or their fragments in the range of the size of bases to megabases) with variable concentrations of copolymers in the range of 2-30% by weight to about 5-20% by weight.
The unique discrete and discontinuous multiple arrangement of molecularly specific, e.g., specific macromolecules and/or . .

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sizes of macromolecule~, retaining filters or sieves con~titutes the basic principle of this invention.
;~ For the purpo6es of the specification and the appended , claims it will be understood that the term "discontinuous" in .
relation to the system of the invention is intended to mean that the specific values Of T, KD and/or KR for adjacent separating i gel-layers or 8ieVQ8 of the system will not be related in any ' sequential linear, exponential or logarithmic fashion, but rather, will differ by a statistically significant (for purposes of separation) nonsequential (i.e., discontinuous) value. This is clearly shown and demonstrat~d in both the examples and the drawing figures.
The procedure for making a discrete system of specific molecular sieves or filters includes: empiric or experimental ~ 15 selection between sieves and specific binders (e.g., based on immuno-affinity, complexing, physical or chemical interactions, etc.) in particular relation to the macromolecules to be isolated. Emp~ric or experimental selection of molecular dimensions of molecular mixtures for which the discrete system should be effective. Empiric or experimental selection of . .
molecular standards and/or markers indicating critical margins of the dissolved molecular spectrum and preparation of discrete multitudinous sy~tems of specific, discontinuous sieves or filters first in a working fo~m of non-polymerized or non~

:~ .
gelatinized status.
The discrete gelous polymers are organized into discrete multiple layPrs~ each of them designed to have exclusive and/or strictly specific properties, which are selectively effective in ,. ...
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the molecular separation and/or in molecular characterization of ~,~ the chosen mixture of ~acromolecules.
In one embodiment, the discrete layers of the gel polymers are obtained by polymerization (at elected various degrees) of recrystalli~ed N-methylol-acrylamide with a bifunctional acrylic or allylic compound (e.g., BIS or DAT~) ~orming a three-di-mensional, cros~-linked sieve of the resulting polymer having different acrylamide monomer concentrations, e.g., 36% of the acryl monomer. To activate the above reaction, a free-radical catalyst, e.g., potassium persulfate, riboflavin or TEMED, was added, a~ well as a sodium chloride and/or TRIS, or barbitalp or ~, ~ similar buffer solution to provide the intended concentration of acrylamide monomer (T).
Usually, each layer is prepared individually and casted ~i 15 (layered) into pre-prepared glass column, capillaries, circular `;~f'.. ~ dishes, or pre-asse~bled macro- or micro-plates in the molds~
Where indicated, layers containing gradients are prepared using conventional linear or exponential gradient makers (e.g., LKB
217-MULTIPHOR II, or others). Repel Silane and/or Bind Silane (LKB) may be applied, if the gels are to be bound or repelled off .` the glass plate.
Where needed, the above-described PAA gels may be modified - and/or filled with agarose, sepharose, agarose derivatives~ etc.
~ (as demonstrated in the Examples).
f 25 The following abbreviations have the meaning indicated:
ACRYL = Acrylamide BIS = N~N'-methylenebisacrylamide ....
`f.;' DATD = N,N'-diallyltartardiamide . , ¢ ~ SUBSI~ITUTE SHE~
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~` - 16 -DMAPN = 3-dimethylaminopropionitrile TEMED = N,N,N',N'-tetramethylenedia~ine TRIS = tris thydroxy-methyl~ aminomethane T = range of acrylamide mono~er and bimer concentration in %
~: g ACRYl + g BIS
% T = --~ -- x lO0 .i~ lO0 ml gel PAA = polyacrylamide ' 10 PAAG = polyacrylamide gel(s) s: PAAGE = polyacrylamide gel(s) electrophoresis ~ GPAAGE = gradient polyacrylamide gel(s) :~ electrophoresis ~ ~ C = crosslinking degree , . .
~; 15 g BIS
% C = ----------~ ---- X 1 0 0 g ACRYL + g BIS
Where suitable, the layers are filled starting from the bottom of the molds. The "~tacking gels" are layered as the lask :.~
,:` 20layer.
At the end of this operation, any known techniques can be employed ~or the construction of the applicator gels with precast ~i .~sample wells (capable of absorbing from 5 ~l to 80 ~l sampla ~:volumes).
y~ 25Construction o~ the final system of specific layers is :~prepared in any quantity, quality, physical form, composition and ~ :
sequence. The borderlines of the layers may be constructed with ~ :
~i;5 .sharp, visible edges tfor example if the previous layer is fully . -~gelatinized, when the following on~ is being overlaid) or in .~;; 30 unbroken confluent fashion.

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Semiliquid or liquid gelous forms which do not change their physical status in tlme, although fully polymerized, have to be fitted into their containers (o~ any shape) in order to prevent a mixing of materials.
The separation lines of the above layers (of the same, or different concentrations and/or compositions) are rectilinear when electrophoretic plates or columns are slected or strictly circular (when circular microelectrophosis, or circular di~fusion techniques are picked-up~.
All possible mixtures of gelatinizing materials, with or without a~y additional i'filling" gels, can be used without - restrictions.
, The discrete layers may be particularly efficient as system : of almost identical or widely varying layers, because of their optimal composition, structure or length or shape or gradation ; of changes in their quality or composition.
,:
The final shape of these discrete systems of molecular sieves may be manufactured in any thickness and usable forms in ` large or small (e.g. microplates) plates with glasseous or plastic supports, columns in glass tubes, or in capillaries, ... .
!; chromatogr~phic columns, etc.
In this way, gel-plates of various sizes (from 125 x 250 mm, allowing the simultaneous separation of up to 96 biological ;~.
~ ~ samples - down to 50 x 50 mm, allowing the simultaneous separ~
:
!,,~ 25 ation of 9 amples and only one standard) may be prepared.
~ ; The preservation of gels in glass plates, containing ¦ analytical systems of discrete and/or discontinuous gelose layers ~ may be preserved by immersion into a buf~er solution as well as ,. .

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by packing them in storage bags. In such manner, it will be possible to handle their shipment and othex usual commercial handling.
The above-described gel-casting forms (e.g. plates, tubes, capillaries, etc.) should be useful with any known electro~
s phoretic procedure.
The optimal composition of the multitudinous, discrete~
nonsequential gel layers may be prepared or developed by determi-nation of partition (KD) and/or retardation (KR) coefficients, ~ l0 calculated for the selected molecular groups of the analyzed ;~ mixture.
All known forms of low or high volta~e (vertical, horizon-;~ tal, radial, capillary, etc.) electrophoresis, electrofocussing, ~ chromatography, etc. may be utilized for separation o~ macro ., molecules. The mode of distribution o~ mixed macromolecules ~ throughout the mediae may also be strongly dependable on the v available instrumentation. However, once the best fitting s instrumentation is established, it may be easily standardizedO
Specific electrophoretic mA, V, V-hrs. (buffer strength, pH
continuity, discontinuity, etc.) have to be established. For the separation of genetic material a Pulse Field Electrophoresis may also be employed.
The unique discrete and discontinuous nonsequential and multitudinous arrangement of molecularly specific (e.g. specific for certain macromolecules and/or sizes of macromolecules) molecular filters or sieves cons~ructed in agreement with separa-r~ ~ tion coefficients, constitutes the basic principle of this ~ invention.
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-: 19 FIG. I depicts compositions of discrete sequential (A,B) and discrete nonsequantial (C-I) systems of different molecular sieves, comprising ~rom layers (l-1~) cf copolymers of acrylamide t3els, wherein each individual layer differs from the adjacent `~ 5 layer.
Nu~ber "0" is assigned to the "sampling" or "stocking gel"
equipped with sample holes.
Letter "T" indicates % T, e.g.. range of acrylamide comonom-. ers concentration in percent, utilized in gels (represented in 10 that particular column).
Letters A-I characterize system of gels, sampled in each . column, every diPferent from each other.
Shaded fields in the said columns F-1 represent in~o P~A
filled materials:
. 15 column F = agarose with or without distantly layers :~ column G = linear gradient PAAG
column H = different discrete non-sequential P~AG with ~. their distantly layers :~ column I = with a layer of immunofixative material.
~ 20 FIG. II demonstrates a natural polydispersed macromolecular ~: system (e.g., pre-isolated serum lipoproteins by ultracentri~uga~
~ tion) separates into 5-6 groups (= fractions) on discrete poly-~:.
: acrylamide gels with se~uentially increasing concentrations~ but . into 12-13 groups and subgroups (classes and-subclasses) on the .::
discrete, nonsequential and multitudinous agarose-polyacrylamide gels, forming si~ves with speci~ic partition coefficients for .:: their class2s and most important subclasses.
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,~ - 20 -s,Number "0" is assigned to the "samplingl' g~ls, equipped with sample hole6.
Numbers 1-14 indicate amount of layers.
Letter "T" indicates ~ T, e.g., range of acrylamide monomers concentration in percent, utilized in particular gels.
Abbreviation "GRAD" accounts for discrete polyacrylamide gels with sequentially increasing concentrations.
Abbreviations'lDISCI'accounts ~or a discrete, nonsequential~
discontinuous and multitudinous polyacrylamide-agarose gels, forming sieves with speci~ic partition coefficients for lipoproteins.
Letters "a - 1" represent related individual groups and/or , subgroups of the identical molecular system, separated for a $,~ comparison on "GRAD" and 'IDISCIl analytical systems. The j~ 15 superiority of the analytical potential of the "DISC~ system is clearly documented.
-s~
FIG. III deplcts a glass plate in homogeneous electric fi@ld with adhering system of discrete nonsequential gelous molecular sieves, build according to the methods described under EXAMPLE
s, 207, in compliance with partition (KD) and retardation (RR) coeffi-,- cients for analyzed lipoprotein classes and diagnostically :f`. .
~, important subclasses.

~; The separating layers (empty intervals) alternate with ''~ distantly layers (shaded intervals).

25Alphabetically arranged set of the letters ~to the le~t~

correspond to the letters used ~or characterizing individual ., .
- classes and subclasses of serum lipoproteins described under ..
.~ :

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r EXAMPLE 7. They are related to standard abbreviations of the lipoprotein classes and subclasses ~to the right).
The following nonlimiting examples may contribute to the more clear understanding of the present inv2ntion.

i '.
E X A M P L E S
The following examples illustrate the use of the sy~tem of molecular sieves with specific retention gradients described in this invention.

E X A M P h E
The organization of discrete molecular sieves with sequen , ~ .
tially increasing concentration of the copol~mer has no different sieving effect than have the corresponding well known i'gradient :: .
~ gels" as it depicted on FIG. I, column A'.
$ For example, the polyacrylamide gels arranged into discrete, but sequential order like into a system of gels of sequent.ial concentrations from 1%, 2%, 3%, 4% 5%, 6~, 7%, 8%, 9%, 10%, ll~, . .
12%, 13%, 14%, have been used.
- The gel mixture for each layer of gel is prepared separate-~ ly. For the preparation of individual gel layers was provided a .~ .
basic solution which contained l.6 ml of ~RIS-borate Na2 EDTA
(TBE) buffer (pH = 8.3) l.0 ml of 6.4% 3-dimethylaminopropioni-trile (DMAPN) and 4.25 ml of a acrylamide-bisacrylamide solution .... ..
All examples (l 9~ refer to the FIG. I.
Number "0" is assigned to the "sampling" or "stacXing gel", equipped with sample holes. Letter "Tt' indicates % T, e.gO, range of acrylamide comonomers concentration in percent, utilized ` in gels (represented in that particular column).
- Letters A-I characterize gels, sampled in each column, different from each other.
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which concentration corresponded to those, defined for the given gel-layer on FIG. I, ~olu~n ~. Distilled and deionized water is added to each gel mixture, in order to adjust the total volume to 15.0 ml.
0.5 ml of 1.6 % ammonium persulfate is added to each of the i gel mixtures, so that the speed of the transfer to the gel mould ~ is quick. The components are mixed and the selected portion (loO
., ml) is carried over into the casting form, but not before the ~; previous layer has gelified.
;~ 10 The TBE buffer solution was prepared from 32.7078 g of TRIS~
~ 14.8332 g of boric acid and 3.35025 g of Na2 EDTA, dissolved in ~ 3,000 ml of deionized water, after adjustment to the pH 8.3 with `~ 2N HCl. The 6.4% DMAPN solution was prepared by resolving 16 ml of concentrated DMAPN in 250 ml of deionized water. The acrylamide- bisacrylamide solution was prepared by adding 47O5 .: g acrylamide to 2.5 g N,N'-methylenebisacrylamide in 250 ml of ~ deionized water. The ammonium persulfate solution was prepared i by adding 1.6 g ammonium persulfate in 100 ml of deionized water~
~-` (All chemicals were obtained from Sigma Chemicals).
The serum lipoproteins were pre-isolated by ultracentrifuga~
tion at density 1.21 g/ml, adjusted by NaCl and KBr, using . Beckman Model L Ultracentrifuge at 100 000 g for 16 hours in a . Beckman type 50 rotor.
The electrophoresis of lipoprotein flotants and molecular : 2S weight standards was performed in a 10 cm long, 10 cm large and ~:~ 2.0 ~m thick vertical slab gels ~casted in GSC-8 apparatus) at 14-C for the period of 16 hours, 1 hour at 30 V and 15 hours at :
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WO92~10277 2 ~ 9~ PCT/US91/~3 , constant voltage of 125 V, in TBE buffer tpH = 8.3), utilizing Pharmacia- Uppsala analytical vertic~l system (GE-2/4-LS cell).
The lipoproteins separated in gels were pxestained (by mixing of equal volume of flotant with 5 g per L solution of Sudan Black B in ethylene glycol), or stained after a standard .' fixation (200 g trichloracetic acid in 1000 ml of distilled water) with Coomassie Blue G 250 for apolipoproteins (in a mixture of equal portions of acid copper sulfate solution and .~ solved Coomassie Blue in diluted me hanol), or Sudan Black B for .. 10 lipidic moiety (using SBB in ethyleneglycol or in 60% ethanol) 9 ' ~ respectively Oil red 0 stain. The gels were therea~ter destained .~ .
a preserved in adequate preserving solution ~ or dried.
There were also employed molecular weight standards:
Carboxylated Latex Beads of molecular weight 5,388,333 d (DOW
Chemicals), Thyreoglobulin dimer of molecular weight 1,4000 d(Phamacia) and Thyreoglobulin monomer of molecular weight 669,000 d tPharmacia), with Ferritin sf moleculax weight 440~000 , .~ . .
d (Sigma, Chemical Company~, Catalase of molecular weight 230,000 d (Behringer Mannheim~, Aldolase (isolated from muscle) of ;~ 20 molecular weight 158,000 d (Calbiochem), and Bovine serum albumin ~: of molecular weight 66,250 d (Pharmacia). The results, obtained :::
~ by the sep~ration of serum lipoproteins on the above-described "5- discrete polyacrylamide gel layers with se~uentially increasing ,;.
s concentrations (demonstrated from T = 1%, 2%, 3%, 4%, 5%, 6%~ 7%~
8%, 9~, 10%, 11~, 12%, 13~, 14%) to 16% or even 27~, respectively :
j were really not more success~ul, than it has been previously ." experienced using for example 1% or 1.2% agarose gels.

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- 24 ~
The serum lipoprotein separated into the following ~ractions (see FIG. II, column GRAD):
, a = contains chylomicrons (CHY) and their fragments :~ (W LDL), i, 5 b = contains very low-density lipoproteins (VLDL~
c-j = co~plex of unseparated lipoprotein classes and subcla~ses of low density lipoprotein group .~ (LDL), h = consists most probably from a medium density lipoprotein (MDL), and - i-l = complex of unseparated lipoprotein group of high~
density lipoproteins (HDL~, characterized b~ a long, trailing fraction.
. The insufficiency of the discrete polyacrylamide gels with~ : 15 sequentially increased concentrations separation of 5erum lipoproteins was evidently demonstrated.
:
. .
. E X A M P L E 2 An analytical system of eleven discrete polyacrylamide gels J, with sequenti-ally increasing concentrations was prepared ~: 20 according to the method given in EX~MPLE 1, as delineated on FIGo ~: I, colu~n B. However, the original layer numbers 3-5 were substituted with one longer gel layer characterized by T - 3% and ., .
layer numbers 7-9 by an another longer layer of T ~ 5% . More . spacious gel layers with critical concentration of T = 3% and $~ 25 T = 5~ offered significantly better separation of ;.; biomacromolecules of molecular weight ranging from 4-2,000,000 ; d and from 800,000-1,300,000 daltons.

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WO92/10277 ~O9~ PCTJUS91~ ~8 , ~ This gel system was also further technically adju~ted so that between lay~r n~mbers 2 and 3, as well as 3 and 4, 4 and 5, . 5 and 6, 6 and 7, 7 and 8, 8 and 9 and finally 9 and 10 were .~ inserted standard gel layexs of a standard concentration of co-monomers expressQd by T = 2.5~.
.j In this manner, the resolution potential of the above-~:: described system was significantly enhanced.

An analytical system of twelve discrete polyacrylamide gels with nonsequentially increased concentrations of co-monomers was ~: prepared according to the method given in EXAMPLE 1, as $ :. illustrate on FIG.I, column C with the extraordinary arrangement ~ that the differences in the concentration of comonomers were ¦ decreased to 0.2% T in the upper part of the system. To the contrary, the following gel layer, characterized T = 3% was three . . .
.~ times lengthened in order to facilitate better separation of biomacromolecules of molecular weight ranging from lO,OOO,OOC-~i 2,500,000 daltsns. The lower part of system was arranged into a .
~;~ so called nonsequential ~orm o~ gels characterized by ~ 20 nonsequential increasing concentra~ions of co-monomers, expressed ;.; by nonse~uential values o~ the index T%.
~:: The above introduced discrete gelose sieves possess distin-guished and very specific composition, sy~bolized by ¦ independently irregular, but relatable specific indexes T%. They , 25 were intentionally prepared in ~he way to meet specific i conditions, under which the density of their network will , ,- ., .
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~ selectively allow the proper partition and retardat.ion of .. intended biomacromoleculss.

. . .
' E X A M P L E 4 A system of discrete, ~ultitudinous, nonsequential gelous 5 molecular sieves wa~ prepared according to the design placed into column D, FIG. I and methods given in EXAMPL~ 1. The specific.ity . of this arrangement is based on an insertion of a polyacrylamide ;, s gradient gel-plate with linearly increasing concentration from
4 to 6% T into the position 8-11.
This assortment allowed an improved separation o ;~: biomacromolecules in the range of 800,000-1,500,000 daltonsO~.' :~: E X A M P L E 5 ~ For the successful separation of viral components and thelr Sr fragments was prepared a system of discrete, multitudinousi~
nonsequential gelous molecular sieves according to the design of .' column E, FIG. I and methods, given in EXAMPLE 1.
The gel-layer numbers 2 and 3 were substituted with a gradient polyacrylamide gel with linearly increasing concentration of co-monomers from T = 2% to T = 3%. Similarly the gel-layer numbers 8-lO were replaced with gradient polyacrylamide gels of exponentially increasing concentration from 4-5.8 %T
The above-described set of changes made possible that ."f biomolecules o~ molecular weights in range of 30,000,000-i 25 10,000,000 daltons and in the range of 500,000-1,000,000 daltons :i, separated in a nonstandard manner, some of them more clearly.

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Discrete or nondiscrete (e.g. continual~, but sequential polyacrylamide gradient gels may replace any segmen~ in a system of discrete, nonsequential, multitudinous gels, so far they contribute to the improvement in separation of analyzed biomacromolecules. Their sequence may be adjusted linearly, or ; exponentially. Their length may be variable. Any gelatinizing ' material may be employed.

.
; E X A M P L E 6 . .
A system of multitudinous, discrete, nonssquantial - 10 polyacrylamide gels, in part forming sieves with speciflc ; .
partition coefficients for analyzed macromolecules W2S prepared ,; according to the plan given in column G, FIG. I with khe exception of the layer numbers 3-7, where an another layer of a linear gradient 3.0-4.8% T polyacrylamide gel was inserted~
Although the above-described analytical syste~ provides remarkably good separation of the pre-isolated serum lipoproteins for their classes without the insertion of the 3.0 - 4.8% T layer of the linear polyacrylamide gel (GPAAG), the GPAAG itsel~
r eliminates2 the superior capability o~ the system of molecular ~` 2a sieves selected according to the KD and/or KR f individual ` classes of serum lipoproteins.
r The group o~ intermediate (IDL) and specifically low-density lipoproteins (LDL)3 failed to separate into three subclasses:
LDL~, LD~ and LDL3. To the contrary, the group of very high-den-;~
,:, ,~.,.
2See FIG. II, colu~n GRAD, lipoprotein group d-j.
3See FIG. II, column DISC, lipoprotein group d g.
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W092/1~277 2 0 9 ~ 3 0 ~ PCT~US91/~.~ ' ~- sity lipoproteins (H~L) separatQd into known subcla~ses. ~n $ another proof of selective action of GPAAG.
"~
E X A M P ~ E 7 ~- Particularly for the separation of serum lipoproteins a combined agarose-polyacrylamide system of multitudinous~
:~ discrete, nonsequential molecular sieves was as6emb1ed accord:ing to a design presented in column F, FIG. I. The concentrations of the copolymers in individual gel-layers were adjusted according to the appropriate KD and/or RR in a mode which made possible to separate diagnostically important classes and ~S~ subclasses of lipoproteins of low, intermediate and high :
molecular weight in one analytical system.
The gel mixture for each layer of gel is prepa.rQd separately. A basic solution was provided, which contained ~.o6 ., 15 ml of TRIS- Borate-Na2 EDTA (TBE) buffer (pH = 8.3), 1.0 ml of .~ 6.4% 3-dimethylaminopropionitrile (DMAPN) later replaced w:ith .~ TE~ED (N,N,N',N',- tetramethylenediamine) and 3.9 ml of agarose (or in some experiments of Sepharose 2B, or 4B, or 6B~, plus 4.25 ml of an acrylamide plus bisacrylamide solution which ~ 20 concentration corresponded to those, designed for the given gel-laysr on FIG.I, column F. Distilled and deionized water is added to each gel mixture, in order to adjust the total volume ', to 15.0 ml.
All the above-described gel-mixtures are kept hot in a water-bath up to 90 C, so that the agarose remained soluble.
... .
,`~ Before the use of the above mixture for a preparation of corre i,~. sponding gel-layer, the mixture is removed from the water-bath :,~

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and let cool to 50'-55'C. 0.5 ml of 1.6% ammonium persul~ate is added to ea~h of the gel mixtures, so that the speed of the transfer to the gel mould is quick. The components are mixed and the selected portion (for example 1.0 ml) is carried over into the casting form, but not before the previous layer gelatinized.
Most of the reagents and/or solutions were prepared according to the recipes giv~n under EXAMPLE 1. The 2% agarose ; was prepared according to the following specifications: 2g of Seakem agarose (Marine Colloids, Inc.) was dispersed into 90 ml of deionized, 70 C hot distilled water, cooled to 50 -55 C, adjusted to 100 ml and used, or stored at ~ C.
Serum lipoproteins were pre-isolated, as described under EXAMPLE 1.
Their electrophoresis, fixation, staining, destaining, ; 15 preserving and drying was described under EXAMPLE 1.
The molecular weight standards, used always during separations of biomacromolecules were al~o described previously.
There is no restriction concerning the composition of the basic gelous matrix, not the selection or composition, or concen-tration (0.5% agarose concentration was mostly used in thisinvention) of the filling material. This invention is not restricted to polyacrylamide gels used here only for an example.
Any net forming and well netting material, resembling durable and well consistent gels may be employed for construction of ~, '25 discrete, multitudinous, nonsequential gels in order to construct ~- molecular sieves with specific KD and/or KR for separations of intended groups or mixtures of biomacromolecules.

~,'' SlJBSl~lTUTE S~lEET

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WO92/l0277 ~ 8 ~ ~ ~ `; PCT/US91/09 - 30 - ~
The above analytical gelose systems were prepared al50 as a thin (- 1 . o mm~, or ultra-thin (= 0.5 mm) gels and as well as so called "micro" gels ~5.0 x 5.0 cm). The thinner modifications offPred better separation of lipoproteln subcla~ses, mainly in the group of HDL. Similarly, the formation of electrophoretic zones was improved.

The serum lipoproteins were separated into the classes and subclasses which were verified by their identification ~ith ~^ corresponding classes and subclasses isolated by preparative ultracentrifugation at specific densities and by a reanalysis using analytical micro-ultracentrifugation, as well as by laser ~
light scattering spectroscopy and finally by immunochemical and chemical analyses.
The following separation of serum lipoproteins was achievedo 1. The first very thin and porous gel-layer (of 1. OD1 O 5 y %T) separates out only the largest molecules ~e.g. 1, 000-4f 000 A4, e.g., 100,000,000 d5 to 350,000,000 d molecular weight)~
representing exogenous chylomicrons (C~Y). Usually, they :/
constitute fraction "a" (FIG. II, column DISC), or its ; 20 pre-fraction, if their concentration is extremely high.
The levels of chylomicrons (0.05-0.1 mg%) are in human circulation so low in the fasting stage that they become inseparable from endogenous chylomicrons and/or from their frayments. In this case, only one fraction appears in the T =
2% molecular sieve.

., ~ -- ........ ...

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W092/10277 2 ~ g 8 ~ , Pcr/usg~tog~8 2. The sQcond gel-layer (of 2.0 ~T) is characterized by XR, mostly suitable to retard chylomicrons of hepatic origin of smaller particle size (600-900 A). Under physiological ~; conditions, even the next group of lipoproteins (W LDL) may be separated in this molecular sieve.
3. The third gel-layer (of 2.5 %T) retains the chylomicron frayments, or very very low-density lipoproteins (VVLDL), charac~
terized by a range of sizes (550-450 A) and molecular weights ç (approx. 80,000,000-25,000,000 daltons). Similarly, under physiologic condition, even this class may contribute to the ~- fraction "a", FIG. II.
4. The forth layer (of 3.0 %T) represents the specific molecular sieve with retardation coefficient for VLDL (- very~low density lipoproteins), forming few subclasses in the broad range ç 15 of 600 to 400 A, e.g. in the range of 18,000,000-6,000,000 d ç (mean 12,000,000 d). Under physiologic conditions, this class has only one, but may reveal up to four subclasses in pathologic ~, stages. Fraction b.
5. The fifth layer (of T = 3.2 (+)0.1 %) has to fit for the retardation coefficient for IDL (- intermediate density chylomicrons), diagnostically second most important class in the spectrum of serum lipoproteins. It may belong metabolically to VLDL, but separates on the invented systems of gels well, "r,~ producing up to 2-4 subclasses under pathologir conditions in the range of sizes 350 250 A and molecular weights of 5,000,000 '- 4,500,000 daltons. Inter-layers o~ standard network of T = 2%
i enhances the clarity of separation. Fraction c.
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6. The sixth cluster of layers (in fact a group of layers ranging in their netting from ~ = 3.5-4.5~, eventually combined ~' with interlayPrs of ctandard network of T = 2%) is equipped with retardation coefficients K~, fitting for retardation of all LDL
s 5 (= low density lipoprotein cla6s) subclasse~. The undivided LDI, ~' class includes lipoprotein particles with BiZQS ranging from 260~
210 A and molecular weights from 4,000,000-l,500,000 d. Under physiological, as well as pathological conditions are separable three standard subclasses, using the analytical system described ; lO in this invention: LDL~ (particle size 0 260 A, and 0 mOw~
3,500,000 d), LD~ (part~cle size 0 225 A, and 0 m.w. 2,500,090 d) and LD~ (particle size 0 210 A, and m.w. l,600,000 d). Two specific densities of nettings (T = 3.5% and 3.8%), or even better five den~ities bf nettings (T = 3.3~ 3.5% 3.7%, 3.9% and :;
4.1~) are utilized. Complex d-g.
7 . The seventh layer (of T = 4.8% + 0.2%) is adequate for K~ of MDL (= medium density lipoprotein), whereby also KR and a proper density of netting may be calculated for Lp(a) if desired.
The MDL render particles of size 200-l90 A and of m.wO l,000,000-~- 20 900,000 d and mean density l.076 g/ml. Fraction h.
8. The eighth cluster of selective layers, eventually with combination of intermediate, alternating standard layers, offers ` the separation of the complicated HDL class. There is some confusion in the present nomenclature, however. Therefore, the minimal physical parameters have to be given in order to .,. - .
. .
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~ - 33 --~, correctly describe, which subclasses are in fact determined of .c HDL (d 1.0635-1.210 g/ml) .6 , The HDL~ = 2b (d 1.070-1.100 g/ml) represents a less .~; known lipoprotein class. Protein contsnt 25%. Mean banding . S position 1.090 g/ml. M.W. = 4,200,000 + 200,000. Peak F ~2~ rate , 5.40 ~ 0.30.
The HDL2 = 2a (d 1.100-1.125 g/ml) includes lipoprotein in the size range 160-~40 A. Choles~erol: protein ratio 0,50 ; to 0.40. Protein content 52%. Mean banding position 1.110 g/ml.
.. 10 M.W. = 2,630,000 ~ 100,000. Peak F ~2~ rate 3.15 * 0.06.
`. The HD~ = 3 a-c (d 1.125-1.200 g/ml) represents a lipoprotein of size range from 113-115 A. Cholesterol: protein ratio 0.37-0.14. Protein content 68%. Mean banding position s, 1.145 g/ml. M.W. = 1,770,000 ~ 90,000. Peak F'~2~ rate 1.56 +
0.13.
~:~. The VHDL~ ~mean d <1.250 g/ml), protein content . 0 /80 :~ The VHDL2 ~mean d >1.250 g/ml), protein content ~, The VVHDL (mean banding position 1.342 gtml), protein content 98%. Complex i - 1.
~:~. The system of molecular sieYes consisting from :$~ ~ polyacrylamide gels was calculated ~rom corresponding KD and K~
for HDLI (T = 5.0%), HD~C (T = 11.6~), HD~ (T = 16.3%) and ~HDL
(T = 18.0%). With the polyacrylamideagarose gels we experienced .~ 25 adequate separation of the above subclasses in the gelous sieves c~
of the range of 5.0-8.0% ~, mainly after shorter electrophoretic runs. one of such separations is depicted on FIG. II, column ., In consent with D. W. Anderson, A. V. Nichols, S. S. Pan and F. T. Lindgren: Atherosclerosis, 29, 161-179, 1978.

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, WO9~/l0277 2 0 9 8 3 ~ CT/VS910~

"DISC", fractions "i - 1". (Letters "a - 1" represent related individual groups and/or subgroups of the identical molecular system, separated for a comparison on "GRAD" and "DISC~
analytical systems).
~ 5 The superiority of the analytical potential of the :~ "DISC" system i5 clearly documented.
,~
~ E X A M P L E 8 `- A combined agarose-polyacrylamide system of multitudinous, discrete, nonsequential molecular sieves wa~ constructed according to the design presented in oolumn H, FIG. I. ?
Similarly, as in the pre~ious case, the concentrations og the copolymers in individual gel-layers were adjusted accordi~g .
~:: to the appropriate KD a~d/or ~Ri in a mode which made possible to ,: separate diagno tically important classes and subclasses o~
lipoproteins of low, intermediate and high molecular weight in ,~ one analytical system.
The method of preparation of such gels was given under ~ ~ EXANPLE 7 and 1 with the specification that layer number 11 was '1 replaced with 11.6% T polyacrylamide gel, number lZ with a similar 16.3% T PAA gel and number 13 with 18.0% T PAA gelO
The above-described modification allowed to separate better !, ~ the subclasseq oP HDL: HDLI - ~D~ - HD~.
,. Combinations of plain polyacrylamide gels and those filled .~ with agarose or its derivatives may be utilized, whenever j~ 25 indicated.

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.~ ' ' ' . , ' WO92/10277 2 ~ 9 ~ 3 1~ ~ PCTt~S91/09~ 1 s ,'''~ ' .

;; The sequential, continuous gradients may be placed anywhere into the analytical system of nonsequential, discontinuous layers. They may be linear, or exponential.
The length of the said continuous gradients may be variable and so may be the material, from which the said matrixes may be prepared.
The optimal "filling matrix material" constitutes, for example, of an agarose and/or it derivatives, wherein any of khe . substituents are attached to the agarose molecule via: a . 10 hydroxyl linkage, or an ester linkage, or an ether linkage~ or an amide linkage, or an amine linkage, or an isourea linkage7 or .~ a carbamate linkage.
~ For example, the layer number 14 was replaced in the :~. standard agarose-p~lyacrylamide gel system of multitudinous~
discrete, nonsequential molecular sieves, made according to the project given in the column F, FIG. I.
The replacement o~ agarose was made in the same manner w.ith . ! .
~. a hydrophobic agarose derivative, the ~-aminohexyl-Sepharose 4B
.; (e.g. 1,6-diaminohexanesepharose, attached through the `'.:20 amino-group to Sepharose 4B). It contained 6-1 ~ moles of 1.6 :~ diaminohexane per ml gel (Sigma, Chemical Company). The 2%
derivative of Sepharose 4B was prepared according to the i; following specifications: 0.2 g o~ ~-aminohexyl-Sepharose 4B was ~ dissolved in 9.0 ml of distilled water, heatad to 70 C, cooled .~ Z5 to 50-55 C, adjusted to 10.0 ml with deionized water, cooled and .~ stored in form of a gel at 4 C.

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In the above arrangement, the last molecular sieve possessed the function of a affinity chromatography ~edium. The length of the sieve may be adapted accordingly.

E X A M P ~ E 9 The above-described combination of discrete, discontinuous, nonsequential system of multilayered molecular sieves, formed either from homogeneous or nonhomogeneous substances, may be further combined with remarkable effectiveness with a specific s layer, containing immunofixative material, if there i5 a need for a selective isolation of a chosen component from an analyzed molecular spectrum (see column I of FI~. I).
The said specific layer with immunofixative capabilities has to be equipped with nonfiltrating, non-sieving properties, e.g., releasing particular molecular groups, passing through this matrix.
; Only one selected prGtein, against which the matrix contains specific antibodies, would be precipitated during the primary process o~ separation of the entire molecular spectrum.
For example, the Lp(a) was routinely retained during regular i, . .
- 20 analytical process, the protein-immuno-complex was eluted from .
the above specific matrix and the atherogenic lipoprotein Lp(a~
was separated out ~or further physicochemical studiesO
` While the invention has been explained in relation to its ~ preferred embodiments, it is to be understood that various - 25 modifications thereof will become apparent to those skilled in ~; the art upon reading the specification. Therefore, it is to be understood that the invention disclosed herein is intended to :' ~
. .

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WO 92/10277 2 0 9 8 ~ ~ G PCI/US91/09348 ' ~
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cover such modifications as ~all withi.n the scope of the appe~lded 'jt claims.
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Claims (15)

What is claimed is:
1. The system of multilayered, discrete, discontinuous, nonsequential gels for molecular electrophoresis of biologic particles and macromolecules, comprising at least about five discrete separating gel-layers, wherein each gelous layer is defined by specific seperation coefficients represented by KD and KR for the analyzed group of molecules, which direct the concentration of the medium and density of netting of each gelous layer, and wherein the value of KD and KR for each discrete separating gel layer differs from an adjacent layer by a significant discontinuous and nonsequential value.
2. The system of claim 1, wherein one or more separating gel layers comprise polyacrylamide or derivatives thereof.
3. The system of claim 2, wherein one or more layers of polyacrylamide gel contain agarose, dextran, or derivatives thereof in an amount ranging from about 0.5% to 5.0% by weight.
4. The system of claims 1-3 which contains a layer made from a medium selected from agarose or its derivatives; a mixed polyacrylamide/agarose gel; a bound ;ogams holding a capability of immunofixation or biospecific sorption; or mixtures of agarose or mixed polyacrylamide/agarose with a bound ligand holding a capability of immunofixation or biospecific sorption.
5. The system of claims 1-4 which contains at least one gelous layer in which the concentration of a copolymer as defined by %T is sequentially increasing linearly or exponentially.
6. The system of claims 1-5 wherein one or more distinct intermediate gelous layers having a concentration of acrylamide polymer, ranging in amounts from about 1.5% to 2.5% T are inserted between the separating gels.
7. The system of claims 1-6 comprising gel structures and gel concentrations (%T) which are effective to separate biologic particles or macromolecules comprising polypeptides, serum proteins, or mixtures thereof and their complexes of low, medium and high molecular weight particles which are separated according to their specific partition (KD) and retardation (KR) coefficients.
8. The system of claim 7 which comprises from about 6-30 separating gel-layers with variable concentrations of copolymers in the range of about 2-30% by weight.
9. The system of claims 1-6 comprising gel structures and gel concentrations (%T) which are effective to separate biologic particles or macromolecules comprising serum lipoproteins into their classes and subclasses according to their specific partition (KD) and retardation (KR) coefficients.

WO 92/10277 PCT/US91/0934?
10. The system of claim 9 which comprises from about 6-18 separating gel-layers with variable concentrations of copolymers in the range of about 2-20% by weight.
11. The system of claims 1-6 comprising gel structures and gel concentrations (%T) which are effective to separate biologic particles or macromolecules comprising serum gycoproteins which are separated according to their specific partition (KD) and retardation (KR) coefficients.
12. The system of claim 11 which comprises from about 6-16 separating gel-layers with variable concentrations of copolymers in the range of about 3-27% by weight.
13. The system of claims 1-6 comprising gel structures and gel concentrations (%T) which are effective to separate biologic particles or macromolecules comprising oligonucleotides and nucleic acids and/or their fragments in the range of the size of bases to megabases which are separated according to their specific partition (KD) and retardation (KR) coefficients.
14. The system of claim 13 which comprises form about 5-20 separating gel-layers with variable concentrations of copolymers in the range of about 2-30% by weight.
15. A multilayer molecular filtration system for the separation of a group of molecules which comprises at least about 5 discrete sieving layers wherein each layer is defined by specific separation coefficients represented by KD and KR for the separated group of molecules, and wherein the value of KD and KR
for each discrete sieving layer differs from an adjacent sieving layer by a significant discontinuous and nonsequential value.
CA002098306A 1990-12-13 1991-12-13 Discontinuous and nonsequential polymeric gel systems for separation of macromolecules Abandoned CA2098306A1 (en)

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