CA2095633C - Enrichment method for variant proteins with altered binding properties - Google Patents
Enrichment method for variant proteins with altered binding properties Download PDFInfo
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- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
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Abstract
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered bind-ing properties for their respective receptor molecules is pro-vided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mu-tated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagem-id particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phangemid expression vectors and selected human growth hormone variants.
Description
WO 92/09690 ~ ~ ~ ~ PCT/US91 /09133 ENRK:HAAENT IAETHOD FOR YARiANT PROTE~IS WITH ALTERED BINDING PROPERTIES
FIELD OF THE INVENTION
This invention relates to the preparation and systematic selection of novel binding proteins having altered binding properties for a target molecule. Specifically, this irwentan relates to methods for producing foreign polypeptides mimicking the binding activity of naturally occurring binding partners. In preferred embodiments, the invention is directed to the preparation of therapeutic or diagnostic compounds that mimic proteins or nonpeptidyl mole~es such a hormones, dings and other smaN
moleales, particularly biologically active molea~les such as growth hormone.
BACKGROUND OF THE INVENTION
Binding partners are substances chat speafically bind to one another, usually through noncovalent interactions. Examples of binding partners inGude ligand-receptor, antibody-antigen, drug-target, and enzyme-substrate interactions. Binding partners are extremely useful in both therapeutic and diagnostic fields.
Binding partners have been produced in the past by a variety of methods including; harvesting them from nature (e.g., antibody-antigen, and ligand-receptor pairings) and by adventitious identification (e.g.
traditional drug development empbying random screening of candidate molecules). In some instances these two approaches have been combined. For example, variants of proteins or pdypeptides, such as polypeptide fragments, have been made that contain key functional residues that participate in binding. These polypeptide fragments, in tum, have been derivatized by methods akin to traditional drug development. M example of such derivitization would include strategies such as cyclization to confortnationally constrain a polypeptide fragment to produce a novel candidate binding partner.
The problem wish prior art methods is that naturally occurring ligands may not have proper characteristics for all therapeutic applications. Additionally, polypeptide Ggands may not even be available for some target substances. Furthermore, methods for making non~naturally occurring synthetic binding partners are often expensive and difficult, usually requiring complex synthetic methods to produce each candidate. The inability to characterize the structure of the resulting candidate so that rational drug design methods can be applied for further optimizatan of candidate molecules further hampers these methods.
In an attempt to overcome these problems, Geysen (Geysen, Immun. Todav. 6:364-369 [1985]); and (Geysen et al., ~~p~p" 23:709-715 [1986J) has proposed the use of polypeptide synthesis to provide a framework for systematic iterative binding partner identification and preparation. According to Geysen et al., Ibid, short polypeptides, such as dipeptides, are first screened for the ability to bind to a target molecule. The most active dipeptides are then selected for an additional round of testing comprising linking, to the starting dipeptide, an additional residue (or by internally modifying the components of the original starting dipeptide) and then screening this set of candidates for the desired activity. This process is reiterated until the Minding partner having the desired properties is identified.
The Geysen et at. method suffers from the disadvantage that the chemistry upon which it is based, peptide synthesis, produces molecules with ill-defined or variable secondary and tertiary structure. As rounds of iterative selection progress, random interactions accelerate among the various substituent groups of the polypeptide so that a true random population of interactive molecules having reproducible higher order structure becomes lays arid less adainabla. For example, inleractians beMleen side drains of amino acki5. which are sequentiasy widely aeparathd but which are spatialH nelgt,mrs. freely ocax.
Furihertnore, sequences that do not taa~tate confartnationally stable secondary sbuGtureS ~~ ~P~x t~et~-ddectr3in interactions wtxcn may prevent sideChaln in6eracifons of a given amino dad with tyre target motearle.
Such complex interactions are -g taalitated by the Nexittility of die polyamlde back 8t ttta pdypeptide candidates. Additionally, rrandldates may exist In numerous aontormAliOrts mafdn9 it dlmCult to identity the oarfartner shat interacts or binds to the target with greatesl alenity or speatrcity eamplicating rational drug des'sgn-A final problem with the ipsrative polypeptide method of Geyttett is that, at presenl, there are r~
practical medrods with which a great diversity of dllferent peptides can be pnvduoed. screened and analyzed. i3y 10 using the Iwenty naturatty Qoaming amino acids, the total number of all oambinatbrrs of hexapeptides that must be synthesized is 64,t>00,000. Even having prepared such a dversity at peptides, there are no methods avrailabte with which mixtrxes of sub a diversity of peptides aan ~ raP~Y Keened to selea those pepdde5'havir~g a high atkniiy for the target molecule. At present, each 'adtrerent' peptide must be recovered in amounts large enough tQ cony out protein sequenoin9~
15 To overcome marry et the problems inherent in the ~y~n aPP~ ~~i~l selection and 5aeenlng was ciwsen as an attemadve. Biological selections and saeens are Powerful foals to probe Protein function and to isolate variant proteins with desirable properties (Shortle, plpi!»Dg. Oxender and Fox, ads., A.R. Liss, Inc., NY, pp.103~108 [1988]) and 8ovne et or.. ., ~T:1306-1310 [199D)).
However, a given selection or gcreen is appliGabIB t0 only Dne or a small number of related proteins.
20 Recendy, umilh and coworkers (Smith. ~~. x:1315~1317 [1985)) arKi Parmley and Smith. ~.
7a:3a5-318 [1985] have der~nstrated that small protein tra9ments (10~50 amino adds) can be 'displayed' etfidently on the surface of iilamentous pf~ge by inserting Short gene lsagmerns Into genQ III of the td phage (~tusion phage'). The gene Ill minor coat protein (present in abo~ 3 ~Ples at one end at the virion) is important for proper pttage assembly and tar infection by attachment to the pill of E
Colt (see Ranched et at. , 25 gsy"50: a01-427 [1886]). Recently, 'fusion pttage' have been shown to be useful for displaying start mutated peptide sequences for identifying peptides Ihat may react with anUbadies (Scott et 8i., 249: 386-390, [1990] -..)and Gwirla et tit., prn~ Na_N, p,ad t A 87: 638~6382. [1990[).or a foreign protein (Llevlin et al"
~~' ,pig, 24~: 404-406 [t990)).
There are, trowever, several important limitations In using tttxh'fusian ptrage' to ider>diy altered 30 pepCides or proteins with new or enhanced birxJitlg fxapeTt~s. Frst, it has teen shown (Parmldy et al., one. 73:
305-318. [1998]) that fusion phage are useful only br displaying proteins of less Ihan tOQ arid preferably less than 50 amino aad residues, becauso large inserts presumably disrupt the krrction of Gene Ill and ii~refore phage assembly and intectivity. Second, prior art methods have been unable b select pep4des from a Gtxary having the highest binding aeiNty for a target molecule. For example, attar exhaustive panning of a random peptide litxary 35 with an antr~ endorphin monoGOrral antiltcdy, t~lrla et al., supra could not separate moderate adinity peptides (icd - 10 wM) from higher affinity peptides (Kd -d.4 ~M) fused id phage. Moreover, the Parent ~_ endorphin peptide sequence which has very trigh aftirtity (iCd ~ 7nMj, was not Padre from the epitope library.
Ladner WO 9010x802 discloses a method for selecting novel birxkrtg proteins displayed on the ouoer surface of cells arid viral particles where it is contemplated drat the heharobgorss proteins may have up to 1 B4 WO 92/09690 ~ ~ ~ PCT/U591 /09133 3 >._ amino acrd residues . The method cont~nplates isolating and amplifying the c~splayed proteins to engineer a new family of Minding proteins having desired affinity for a target moleade. More sped6calty, Ladner discloses a 'fusion phage' displaying proteins having 'initial protein finding domains' ranging from 46 residues (cramt~n) to 164 residues (T4 lysozyme) fused to the M13 gene III coat protein. Ladner beaches the use of proteins'no larger than necessary t~erause it is easier to arrange restriction sites in smaNer amino add sequences and prefers the 58 amino add residue bovine pancreatic trypsin inhibitor (BPTI). Small tusan proteins, such as BPTI, are preferred when the target is a protein or macromolecule, while larger fusion proteins, such as T4 lysozyme, are preferred for small target molecules such as sterads because such large proteins have clefts and grooves into which small molecules can fit. The preferred protein, BPTI, is proposed to be fused b gene III at the site disclosed by Smith et al. or de la Cruz et al., J. Biol. Chgm" 263: 4318,4322 [1988), or to one of the terminii, along with a second synthetic copy of gene III so that'some' unaltered gene III protein will be present. Ladner does not address the problem of successfully panning high affinity peptides from the random peptide library which plagues the biological selection and screening methods of the prig art.
Human growth hormone (hGH) partidpates in much of the regulation of normal human growth and development. This 22,000 dalton ptuitary hormone exhibits a multitude of biological effects including linear growth (somatogenesis), lactation, activation of macrophages, insulin-like and diabetogenic effects among others (Chawla, R, K. (1983) 911p ev. Med. fig, 519; Edwards, C. K et al. (1988) ,~,p~,~Q, 769; Thomer, M. 0., et al.
(1988) J. Clip. Invest gl, 745). Growth hormone defiaency in children leads to dwarfism which has been successfully treated for more than a decade by exogenous administratan of hGH.
hGH is a member of a family of 2 0 homologous hormones that include placenhal lactogens, prolactins, and other genetic and spades variants or growth hormone (Nicoll, C. S., efal., (1986) ~ocrine Reviews 2,169). hGH is unusual among these in that it exhibits broad species spedfidty and binds to either the dor~ed somatogenic (Leung, D. W., et aL, [1987] ~g ~,,3,Q, 537) or prolactin receptor (Boutin, J. M.,et al., [1988] fig; ,~, 69). The doped gene for hGH has been expressed in a secreted forth in ~j (Ctrarg, C. N., et al., [i987] ~;~,189) and its DNA and amino add sequence has been reported (Goeddel, etal., p979] ~,~, 544; Gray, etal., [1985] ~,3$, 247).
The three-dimensional stnxture of hGH is not available. However, the three-dimensional folding pattern for porcine growth hormone (pGH) has been reported at moderate resolution and reffr~ement (Abdel-Meguid, S. S., et al., [1987j Proc-Natl.Natl.
e~~d. Sci. USA $4, 6434). Human growth hormone's receptor and antibody epitopes have been identified by homolog-scanning mutagenesis (Cunningham etal., Saenoe Zq$;1330,1989). The structure of novel amino terminal methionyl bovine growth hormone contair>ing a spliced-in sequerxe of human growl hormone including histidine 18 and histidine 21 has been shown (U.S. Patent 4,880,910) Human growth hormone (hGH) cages a variety of physiological and metabolic effects in various ar>imal models including linear bone growth, lactation, activation of macrophages, insulin-like and diabetogenic effects and others (R. K. Chawla etal., Anna. Rev. Mad. 34, 519 (1983); 0. G. P. Isaksson etat., Mrw. Rev. Phys'rot. 47, 483 (1985); C. K. Edwards etal., Science 239, 769 (1988); M. 0. Thomer and M. L.
Vance, J. Clip. Invest. 82, 745 (1988); J. P. Hughes and H. G. Friesen, Mn. Rev. Physiol. 47, 469 (1985)).
These biological effects dertve from the interaction between hGH and spedfic cellular receptors..
Accordingly, it is an object of this invention to provide a rapid and effective method for the systematic preparation of candidate binding substances.
It is another object of this invention to prepare candidate Minding substances displayed on surface of a phagemid particle that are conformationally stable.
It is another object of this invention to prepare candidate tending substances comprising fusion proteins of a phage coat protein and a heterologous polypeptide where the polypeptide is greater than 100 amino acids in length and may be more than one subur>;t and is displayed on a phagemid particle where the polypeptide is encoded by the phagemid genome.
It is a further object of this invention to provide a method for tt~e preparation and selection of binding substances that is suffiaently versatile to present, or display, all peptidyl moieties that could potentially particlpate in a nonoovalent binding interaction, and to present these moieties in a fashion that is sterically confined.
Still another object of the invention is the production of growth hormone variants that exhibit stronger affinity for growth hormone receptor and binding protein.
It is yet another ot~ject of this invention to produce expressan vector phagemids that contain a suppressible termination oodon tunc6onally bcated between the heterok~gous polypeptide and the phage coat protein such that detectable fusion protein is produced in a host suppressor cell and only the heterologous polypeptide is produced in a non-suppresser host cell.
Fnally, it is an object of this invention to produce a phagemid particle that rarely displays more than one copy of candidate binding proteins on the outer surface of the phagemid particle so that efficient selection of high affinity tHnding proteins can be achieved.
These and other objects of this irnention wit be apparent from consideration of the invention as a whole.
These objectives have been achieved by providing a method for seleding novel binding polypeptides comprising: (a) constructing a replicable expression vector comprising a first gene encoding a polypeptide, a second gene encoding at least a portion of a natural or wild-type phage coat protein wherein the first and second 2 5 genes are heterologous, and a transcription regulatory element operady linked to the first and second genes, thereby forming a gene fusion erxxxfing a fusion protein; (b) mutating the vector at one or more selected positions within the first gene thereby forming a family of related plasmids; (c) transforming suitable host peas with the plasmids; (d) infecting the transformed host cells with a helper phage having a gene encoding the phage coat protein; (e) culturing the transformed infected host cells under conditions suitable for forming recombinant phagemid particles containing at least a portion of the plasmid and capable of transforming the host, the conditions adjusted so that no more than a minor amount of ph~emid particles display more than one copy of the fusion protein on the surface of the particle; (f) contacting the phagemid particles with a target molecule so that at least a portion of the phagemid particles find to the target molecule; and (g) separating the phagemid particles that bind from those that do not. Preferably, the method further comprises transforming suitable host cells with recombinant phagemid particles that bind to the target molecule and repeating steps (d) through (g) one or more times.
Additionally, the method for selecting novel binding proteins where the proteins are composed of more than one subunit is ad~ieved by selecting novel binding peptides comprising constructing a replicable expression vector comprising a transcription regulatory element operably linked to DNA
erxoding a protein of interest WO 92/09690 ~ ~~ ~ ~ ~ ~ ~ PC1'/US91/09133 _ containing one or more subunits, wherein the DNA encoding at least one of the subunits is fused to the DNA
encoding at least a portion of a phage coat protein;mutating the DNA encoding the protein of interest at one or more selected positions thereby fomring a family of related vectors;
transforming suitable host cells with the vectors; iMecting the transformed host cells with a helper phage having a gene encoding the phage coat protein;
5 culturing the transformed infected host cells under conditions suitable for forming recombinant phagemid particles containing at least a portion of the plasmid and capable of transforming the host, the conditions adjusted so that no more than a minor amours of phagemid particles display more than one copy of the fusion protein on the surface of the particle; contacting the phagemid particles with a target molecule so that at least a portion of the phagemid particles bind to the target molecule; and separating the phagemid particles that hind from those that do not.
Preferably in the method of Ihis invention the plasmid is under tght control of the transcription regulatory element, and the culturing conditions are adjusted so that the amount or number of phagemid particles displaying more than one copy of the fusion protein on the surface of the particle is less than about 1 %. Also preferably, amount of phagemid particles displaying more than one copy of the fusion protein is less than f0% the amount of phagemid particles displaying a single copy of the tusan protein.
Most preferably the amount is less than 20°x.
Typically, in the method of this invention, the expression vector will further contain a secretory signal sequences fused to the DNA enaxling each subunit of the polypeptide, and the transcription regulatory element will be a promoter system. Preferred promoter systems are selected from; Lac Z, a,pL, TAC, T 7 polymerise, tryptophan, and alkaline phosphatase promoters and combinatans thereof.
Also typically, the first gene will encode a mammalian protein, preferably the protein will be selected from; human growth hormone(hGHj, N-metfionyl human growth hormone, bovine growth homrone, parathyroid hormone, thyro>one, insulin A-drain, insulin B~chain, proinsuWn, rolaxin A~hain, relaxin B-chain, prorelaxin, glycoprotein hormones such as follicle stimulating hormone(FSH), thyroid stimulating hormone(TSH), and leutinizing hormone(LH), glycoprotein hormone receptors, caldtonin, glucagon, factor VIII, an antibody, lung surtactant, urokinase, streptokinase, human tissue-type plasminogen activator (t-PA), bombesin, factor IX, thrombin, hemopoietic growth factor, tumor r~sis factor-alpha and -beta, enkephalinase, human serum albumin, mullerian-inhibiting substance, mouse gonadotropin-associated peptide, a microbial protein, such as betalactamase, tissue factor protein, inhibin, activin, vascular endothelial growth factor, receptors for hormones or growth factors; integrin, thrombopoietin, protein A or D, rheumatoid factors, nerve growth factors such as NGF-)3, platelet~rowth factor, transforming growth factors (TGFj such as TGF,alpha and TGF-beta, insuiin-like growth factor-I and -II, insulin-like growth factor binding proteins , CD-4, DNase, latency assodated peptide, erythropoietin, osteoinductive factors, interferons such as interferon-alpha, -beta, and -gamma, colony stimulating factors (CSFs) such as M-CSF, GM-CSF, and G-CSF, interleukins (ILs) such as IL-1, IL-2, IL-3, IL-4, superoxide dismutase; decay accelerating factor, viral antigen, HIV
envelope proteins such as GP120, GPt40, atrial natriuretic peptides A, B or C, immunoglobulins, and fragments of any of the above-listed proteins.
Preferably the first gene will encode a polypeptide of one or more subunits containing more than about 100 amino add residues and will be folded to form a plurality of rigid secondary structures displaying a plurality of amino acids capable of interacting with the target. Preferably the first gene will be mutated at codons corresponding to only the amino acids capable of interacting with the target so that the integrity of the rigid secondary strur.~ures will be preserved.
Normally, the method of this invention will empby a helper phage selected from; M13K07, M13R408, M13-VCS, and Phi X 174. The preferred helper phage is M13K07, and the preferred coat protein is the M13 Phage gene III coat protein. The preferred host is E. cbli, and protease deffdent strains of E. coli. Novel hGH
variants selected by the method of the present irnentan have been detected.
Phagemid expression vectors were constnxted that contain a suppresside termination colon functionally located between the nucleic acids encoding the polypeptide and the phage coat protein.
BRIEF DESCRIPTION OF THE FIGURES
FIGURE 1. Strategy for displaying large proteins on the surface of filamenbous phage and enriching for altered receptor binding properties. A plasmid, phGH-Ml3glll was consrruucted that fuses the entire coding sequence of hGH to the carboxyl terminal domain of M13 gene III. Transcription of the fusion protein is under control of the lac promoberloperator sequence, and secretion is directed by the stll signal sequence. Phagemid partices are produced by infection with the 'helper phage, M13K07, and particles displaying hGH can be enriched by twining to an affinity ma~ix contakring the hGH receptor. The wild-type gene III (derived from the M13K07 phage) is diagramed by 4-5 copies of the multiple arrows on the tip of the phage, and the fusion protein (derived from the phagemid, phGH-Ml3glll) is indicated schematicatiy by the folding diagram of hGH repladng the arrow head.
FIGURE 2 knmunot~lot of whole phage particles shows that hGH comigrates with phage. Phagemid 2 0 particles purified in a cesium chloride gradient were loaded into duplicate wells and electrophoresed through a 1 agarose gel in 375 mM Tris, 40 mM glycne pH 9.6 buffer. The gel was soaked in transfer buffer (25 mM Tris, pH
8.3, 200 mM glyane, 20% methanol) containing 2% SDS and 296 ~-mercaptoethanol for 2 hours, then rinsed in transfer buffer for 6 hours. The proteins in the gel were then electrobbtted onto immot>ilon membranes (Millipore). The membrane containing one set of samples was stained with Coomassie blue to show the position of the phage proteins (A). The duplicate membrane was immures-stained for hGH by reacting the membrane with polydonal rabbit anti-hGH antibodies folbwed by reaction with horseradish peroxidase conjugated goat anti-rabbit IgG antibodies (B). Lane 1 contains Ifrre M13K07 parent phage and is viside only in the Coomassie blue stained membrane, since it lacks hGH. Lanes 2 and 3 contain separate preparations of the hormone phagemid partiGes which is visible both by Coomassie and hGH immuno-stairang. The difference in mgration distance between the parent M13K07 phage and hormone phagemid particles reflects the different size genomes that are packaged within (8.7 kb vs. 5.1 kb, respectively).
FIGURE 3. Summary diagram of steps in the selection process for an hGH-phage library randomized at colons 172,174,176, and 178. The template molecules, pH0415, containing a unique Kpnl restriction site and the hGH(Rt78G,1179T) gene was mutagenized as described in the text and electrotransfonned into E. cofi strain WJM101 to obtain the initial phagemid library, Library 1. An aliquot (approximately 2%) from Ubrary 1 was used directly in an initial selection round as described in the text to yield Litxary 1 G. Meanwhile, double-stranded DNA
(dsDNA) was prepared from Library I, digested with restriction enzyme Kpnl to eliminate template badkground, and electrotransformed into WJM101 to yield Library 2. Subsequent rounds of selection (or Kpnl digestion, shaded boxes) followed by phagemid propagation were carried out as indicated by the arrows, according to the WO 92/09690 ~ '~ ,-~~ ~ ~ ~ ~ PCT/US91 /09133 ..
procedure described in the text. Four independent doves from litxary 4G4 and tour independent Bones from library 5G6 were sequenced by dideoxy sequendng. All of these Bones had the identical DNA sequence, corresponding 1o the hGH mutant (Glu 174 Ser, Phe 176 Tyr).
FIGURE 4. Structural model of hGH derived from a 2.8 la folding diagram of porcine growth hormone determined aystallographically. t-ovation of residues in hGH that strongly modulate its binding to the hGH-binding protein are within the shaded drde. Alanine substitutions that cause a greater than tenfold reduction(~), a four- to tenfold reduction (~), or increase (O), or a two- to fourfold reduction (~), in tending affinity are indicated. Helical wheel projections in the regions of a-helix reveal their amptupathic quality.
Blackened, shaded, or noruhaded residues are charged, polar, or nonpolar, respedavely. In helix-4 the most important residues for mutation are on the hydrophilic face.
FIGURE 5. Amino acrd substitutions at positions 172,174,176 and 178 of hGH
(The notation, e.g.
KSYR, denotes hGH mutant 172KI174SI176Y/178R.) found after sequencng a number of doves from rounds 1 and 3 of tt~e selection process for the pathways indicated (hGH elution;
Glycine elution; or Glydne elution after pre-adsorption). Non-functional sequences (i.e. vector badkground, or other prematurely terminated andlor frame-shifted mutarns) are shown as'NF'. Fkxrctional sequences which contained a non-silent, spurious mutation (i.e. outside the set of target residues) are marked with a '+'. Protein sequences which appeared more than once among all the sequerxed doves, but with different DNA sequences, are marked with a '~". Protein sequerxes wtych appeared more than once among the sequenced Bones and with the same DNA
sequence are marked with a "'. Note that after three rounds of selection, 2 different contaminating sequences were found; these Bones did not correspond to cassette mutants, but to previously constructed hormone phage. The pS0643 contaminant corresponds to wild-type hGH-phage (hGH 'KEFR'). The pH0457 contaminant, which dominates the third-round glycine-selected pool of phage, corresponds to a previously identified mutant of hGH, 'KSYR.' The amplification of these contaminants emphasizes the agility of the hormone-phage selection process to select for rarely oocurting mutants. The convergence of sequences is also striking in all three pathways: R or K occurs most often at positions 172 and 178; Y or F occurs most often at position 176; and S, T, A, and other residues occur at position 174.
FIGURE 6. Sequences from phage selected on hPRLbp-beads in the presence of zinc. The notation is as described in Figure. 5. Here, the convergerxe of sequerxes is not predictable, but there appears to be a bias towards hydrophotMC sequences under the most sfringent (Glydne) seledion conditions; L ,W and P residues are 3 0 frequently found in this pool.
FIGURE 7. Se~errces from phage selected on hPRLbp-beads in the absence of zinc. The notation is as described in Figure 5. In contrast to the sequences of Fgure. 6, these sequences appear more hydrophilic. After 4 rounds of selection using hGH elution, two clones (ANHQ, and TLDTI171V) dominate the pool.
FIGURE 8. Sequences from phage selected on blank beads. The notation is as described in Fg. 5. After three rounds of selection with glycine elution, no siblings were observed and a badkground level of non-functional sequences remained.
FIGURE 9. Construction of phagemid fl on from pH0415. This vector for cassette mutagenesis and expression of the hGH-gene III fusion protein was constnxted as follows.
Plasmid pS0643 was constructed by oligonudeotide-directed mutagenesis of pS0132, which contains pBR322 and f1 origins of replication and WO 92/09690 PC1'/US91/09133 expresses an hGH-gene III fusion protein (hGH residues 1-191, folbwed by a single Gly residue, fused to Pro-198 of gene III) under the control of the ~ )~ promoter. Mutagenesis was carried out with the oligonuGeotide 5'-GGC-AGC-TGT-GGC-TTC-TAG-AGT-GGC-GGC-GGC-TCT-GGT-3', which introduced a ~
site (underlined) and an amber stop colon (TAG) following Phe-191 of hGH.
FIGURE 10. A. Diagram of plasmid pDH188 insert containing the DNA encoding the Ight chain and heavy chain (variable and constant domain 1 ) of the Fab humanized antibody directed to the HER-2 receptor. V~
and VH are the variable regions for the Ight and heavy chains, respectively.
Ck is the constant region of the human kappa light chain. CH1G1 is the first constant region of the human gamma 1 chain. Both coding regions start with the bacterial st II signal sequence. B. A schematic diagram of the entire plasma pDH188 containing the insert described in 5A. After transformation of the plasmid into E. cbli SR101 cells and the addition of helper phage, the plasmid is packaged into phage particles. Some of these particles display the Fab-p III fusion (where p III is the protein enk~ded by the M13 gene III DNA). The segments in the plasmid figure correspond to the insert shown in 5A.
FIGURE 11 A through C are cdlectJvely referred to here as Figure 11. The nucleotide (Seq. ID No. 25) sequerxe of the DNA encoding the 4D5 Fab molecule expressed on the phagemid surface. The amino acid sequerx;e of the light chain is also shown (Seq. ID No. 26), as is the amino acct sequence of the heavy chain p III fusion (Seq. ID
No. 27).
FIGURE 12 Enrichment of wild-type 4D5 F~ phagemid from variant Fab phagemid.
Mixtures of wild-type phagemid and variant 4D5 Fab phagemid in a ratio of 1:1,000 were selected on plates coated with the extra-cellular domain protein of the HER-2 receptor. After each round of selection, a portion of the eluted phagemid were infected into E. Qoli and plasmid DNA was prepared. This plasmid DNA was then digested with Eco RV and Pst I, separated on a 5% polyakxylamide gel, and stained with ethidium bromide. The bands were visualized under UV light. The bands due to the wild-type and variant plasmids are marked with arrows. The first round of selection was eluted only under acid conditions; subsequent rounds were eluted with either an acct elution (left side of Figure) or with a humanized 4D5 antibody wash step prior to acid elution (right side of Figure) using methods desalted in Example VIII. Three variant 4D5 Fab molecules were made:
H9t A (amino aad histidine at position 91 on the V~ chain mutated to alanine; indicated as 'A' lanes in Figure), Y49A (amino acid tyrosine at position 49 on the V~ chain mutated to alanine; indicated as'B' lanes in the Figure), and Y92A (amino acid tyrosine at position 92 on the V~ chain mutated to alanine; indicated as'C' lanes in the Fgure). Amino acct position numbering is according to Kabat et al.,(Sequences of proteins of immunological interest, 4th ed., U.S. Dept of Health and Human Services, Public Health Service, Nat'I. Institute of Health, Bethesda, MD (1987]).
FIGURE 13. The Scatchard analysis of the RIA affinity determination described in Experimental Protocols is shovm here. The amount of labeled ECD antigen that is bound is shown on the x-axis while the amount that is bound divided by the amount that is free is shown on the y-axis. The slope of the line indicates the Ka; the 3 5 calculated Kd is l ll(a.
WO 92/09690 2 ~ ~ ~ ~ J 3 PCT/US91/09i33 DETAILED DESCRIPTION OF THE INVENTION
The foNowing discussion will be best iurderstood by referring to Figure t. in its simplest torm, the method of the instant invention comprises a method for selecting novel binding polypeptides, such as protein Igands, having a desired, usuaay high, aiffnity for a target molecule from a library of stnxturally related bindirg polypeptides. The lilxary of structurally related polypeptides, fused Do a phage coat protein, is produced by mutagenesis and, preferably, a single copy of each related polypeptide is displayed on the surface of a phagemid particle containing DNA encoding that polypepade. These phagemid particles are then contacted with a target molecule and those particles having the highest affinity for the target are separated from those of lower affinity.
The high atfir>ity binders are then amplified by infection of a bacterial host and the competitive binding step is repeated. This process is reiterated until polypeptides of the desired affinity are obtained.
The novel binding polypeptides or ligands produced by the method of this invention are useful per se as diagnostics or therapeutics ( eg. agonists or antagonists) used in treatment of biological organisms. Structural analysis of the selected polypeptides may also be used to fadlitate rational drug design.
By 'binding polypeptide' as used herein is meant any polypeptide that binds with a selectable affinity to a target molecule. Preferably the polypeptide will be a protein that most preferably contains more than about 100 amino add residues. Typically the polypeptide will be a t~onnone or an antibody or a fragment thereof.
By 'high affinity' as used herein is meant an affinity constant (Kd ) of <t0-5 M and preferably <10'~M
under physalogical conditions.
By 'target molea~le' as used herein is meant any molecule, rat necessarily a pr~ein, for which it is desirable to produce a ligand. Preferably, however, the target will be a protein and most preferably the target will be a receptor, such as a hormone receptor.
By 'humanized antibody' as used herein is meant an antitxxly in wtich the oomplementarity~ietermining regions (CDRs) of a mouse or other ron-human antibody are graffed onto a human antibody framework. By human antibody framework is meant the entire human antibody excluding the CDRs.
L
The first step in the method of this invention is to choose a polypeptide having rigid secondary structure exposed to the surface of the polypeptide for display on the surface of a phage.
By'polypeptide' as used herein is meant any molecule whose expressan can be directed by a specific DNA sequence. The polypeptides of ttws invention may comprise more than one subunit, where each subunit is 3 0 encoded by a separate DNA sequerxe.
By 'rigid secondary structure' as used herein is meant any polypeptide segment exhibiting a regular repeated structure such as is found in; a-helices, 3fp helices, n-heMces, parallel and antiparallel ~-sheets, and reverse toms. Certain 'non~rdered' structures that lack recognizable geometric order are also included in the definition of rigid secondary structure provided they form a domain or'patch' of amino acid residues capable of interaction with a target and that the overall shape of the stnxture is not destroyed by replacement of an amino acid within the structure . h is believed that some non-ordered structures are comt~inations of reverse turns. The geometry of these rigid secondary structures is well defined by ~ and ~r torsional angles about the a-carbons of the peptide 'backbone'.
WO 92/09690 2 ~ ~ ~ ~ ~ J PCT/US91/09133 The requirement that the secondary stmct~xe be exposed to the surface of the polypeptide is to provide a domain or'patch' of amino aad residues that can be exposed to and bind with a target molecule. It is primarily these amino aad residues that are replaced by mutagenesis that form the 'library' of structurally related (mutant) Minding polypeptides that are displayed on the surface of the phage and from which novel 5 polypeptide ligands are selected. Mutagenesis or replacement of amino acid residues directed toward the interior of the polypeptide is generally avoided so that the overall stinxture of the rigid secondary structure is preserved.
Some replacement of amino acids on the interior region of the rigid secondary stnxriues, especlally with hydrophobic amino aad residues, may be tolerated since these conservative substitutions are unlikely to distort the overall structure of the polypeptide.
10 Repeated cycles of'polypeptide' selection are used to select for higher and higher affinity Minding by the phagemid selection of multiple amino aad changes which are selected by multiple selection cyGes. Following a first round of phagemid selection, involving a first region or selection of amino aclds in the ligand polypeptide, additional rounds of phagemid selection in other regions or amino aads of the ligand potypeptide are conducted.
The cycles of phagemid selection are repeated until the desired affinity properties of the ligand polypeptide are achieved. To illustrate this process, Example VIII phagemid selection of hGH
was conducted in cycles. In the first cycle hGH amino aads 172,174,176 and 178 were mutated and phagemid selected.
In a second cycle hGH amino aads 167,171,175 and 179 were phagemid selected. In a third cycle hGH amino acids 10,14,18 and 21 were phagemid selected. Optimum amino aad changes from a previous cycle may be irxorporated into the polypeptide before the next cycle of selection. For example, hGH amino aclds substitutan 174 (serine) and 176 (tyrosine) were irxorporated into the hGH before the phagemid selection of hGH amino aads 167,171,175 and 179.
From the forgoing it will be appreciated that the amino aad residues that form the binding domain of the polypeptide will not be sequentially linked and may reside on different suburuts of the polypeptide. That is, the Minding domain tracks with the particular secorxiary stnxture at the binding site and not the primary stmcture. Thus, generally, mutations will be introduced into colons erxoding amino acids within a particular secondary structure at sites directed away from the interior of the polypeptide so that they will have the potential to interact with the target. By way of illustration, Figure 2 shows the location of residues in hGH that are known to strongly modulate its t~ir~ding to the hGH-binding protein (Cunningham etaL, 247:1461-1465 (1990]). Thus representative sites suitable for mutagerresis would include residues 172, 174, 176, and 178 on helix-4, as well as residue 64 located in a 'non-ordered' secondary structure.
There is no requirement that the polypeptide chosen as a ligand to a target normally hind to that target.
Thus, for example, a glycoprotein hormone such as TSH can be chosen as a ligand for the FSH receptor and a library of mutant TSH molecules are employed in the method of this invention to produce novel drug candidates.
This invention thus contemplates any polypeptide that hinds to a target molecule, and inGudes antibodies. Preferred polypeptides are those that have pharmaceutical utility.
More preferred polypeptides 3 5 include; a growth hormone, including human growth hormone, des-N-methionyl human growth hormone, and bovine growth hormone; parathyroid hormone; thyroid stimulating hormone; thyroxine;
insulin A~chain; insulin B~hain;
proinsulin; follicle stimulating hormone; calcltorun; leutinizing hormone;
glucagon; factor VIII; an antibody; lung surfactant; a plasminogen activator, such as urokinase or human tissue-type plasminogen activator (t-PA);
t~ombesin; factor IX, thromt~in; hemopoietic growth factor; tumor necrosis factor-alpha and -beta; enkephalinase; a WO 92/09690 ~ ~ ~'~ ~ ~ ~ PCT/US91/09133 saran albumin such as txunan seem albumin; mullerian-intibiting substance;
rela~dn A~chain; relaxin B~chain;
prorelaxin; mouse gorradotropn-assoaatsd peptide; a microbial protein, such as beidta~tamase; tissue factor protein; inhibin; activin; vascular endothelial growth factor; receptors for hormones or growth factors; integrin;
thrombopoietin; protein A or D; rheumatoid factors; nerve growth factor such as NGF-J3; platelet~erived growth factor; 6broblast growth factor such as aFGF and bFGF; epidermal growth factor; transforming growth factor (TGF) such as TGF-alpha and TGF-beta; insulin-like growth factor-I and -II;
insulin-like growth factor binding proteins; CD-4; DNase; latency assodated peptide; eryttxopoietin;
osteoinductive factors; an interferon such as interteron-alpha, -beta, and -gamma; colony stimulating factors (CSFs), e.g., M-CSF, GM-CSF, and G-CSF;
interfeukins (ILs), e.g., IL-1, IL-2, IL-3, IL-4, etc.; superoxide dismutase;
decay accelerating factor; atrial natriuretic peptides A, B or C; viral antigen such as, for example, a portion of the HIV ernebpe; immunoglobulins;
and fragments of any of the above-listed polypeptides. In addition, one or more predetermined amino add residues on the polypeptide may be substituted, inserted, or deleted, for example, to produce products with improved tMOlogical properties. Further, fragments of these polypeptides, espedally biologically active fragments, are inducted. Yet more preferted polypeptides of this invention are human growth hormone , and atrial naturetic peptides A, B, and C, endotoxin, subtilisin, trypsin and Other serine proteases.
StiA more preferred are polypeptide hormones that can be defined as any amino add sequence produced in a first cell that binds spedfically to a receptor on the same cell type (autocrine hormones) or a second cell type (non-autocrine) and causes a physiobgical response characteristic of the receptor-bearing cell. Among such polypeptide hormones are cytokines, lymphokines, neurotrophic homnones and aderx>hypophyseal pdypeptide hormones such as growth hormone, prdactin, placer>tal lactogen, luteinizing hormone, follicle-stimulating hormone, thyrotropn, chorbnic gonadotropin, corticotropn, a or ~-melanocyte-stimulating hormone, J3-lipotropin, Y-lipotropin and the endorphins; hypothalmic release-inhibiting honnones such as corticotropin-release factor, growth hormone release-inhibiting hormone, growth hormone-release factor; and other polypeptide hormones such as atrial natriuretiC peptides A, B or C.
IL
The gene encoding the desired polypeptide (i.e., a polypeptide with a rigid secondary structure) can be obtained by methods known in the art (see generally, Samtxook et al. , J
olecular BiojQgw: A Labora~p~"~ap~[, Cold Spring Harbor Press, Cold Spring Harbor, New Yak [19139]). If the sequence of the gene is known, the DNA encoding the gene may be d~emically synthesized (Merrfieki, J. Am. Chem.
Soc.., 85 X149 [1963]). If the 3 0 sequence of the gene is not known, cr if the gene has rot previously been isolated, it may be cloned from a d7NA
litxary (made from RNA obtained from a suitable tissue in which the desired gene is expressed) or from a suitable genomic DNA library. The gene is then isolated using an appropriate probe. For cDNA libraries, suitable probes include monodortal or polydonal antibodies (provided that the cDNA library is an expression library), oiigonudeotides, and complementary or homologous cDNAs or fragments ri~ereof.
The probes that may be used to isolate the gene of interest from genomic DNA litxaries include cDNAs or fragments thereof that encode the same or a similar gene, homobgous genomic DNAs a DNA fragments, and oligorxxleotides. Screening the cDNA or genomic library with the selected probe is conducted using standard procedures as described in d~apters 10-12 of Samtxook et al., supra.
An alternative means to isolating the gene encoding the protein of interest is to use polymerase chain reaction methodobgy (PCR) as described in section 14 of Samlxook et al., sera.
This method requires the use of oligonudeotides that will hybridize to the gene of interest; thus, at least some of the DNA sequence for this gene must be known in order to generate the oGgonudeotides.
After the gene has been isolated, it may be inserted into a suitable vector (preferably a plasmid) for amplification, as described generally in Sambrook et al., supra.
While several types of vectors are available and may be used to practice this invention, plasmid vectors are the preferred vectors for use herein, as they may be constructed with relative ease, and can be readily amplfied. Plasmid vectors generally contain a variety of components including promoters, signal sequences, phenotypic selectan genes, origin of replication sites, and other necessary components as are known to those of ordinary skill in the art.
Promoters most commonly used in prokaryotic vectors include the )~ Z promoter system, the alkaline phosphatase p~ A promoter, the bacteriophage A,PL promoter (a temperature sensitive promoter), the ~
promoter (a hylxid ~-~ promoter that is regulated by the ~ repressor), the Iryptophan promoter, and the bacteriophage T7 promoter. For general descriptions of promoters, see section 17 of Sambrook et al. supra .
While these are the most commonly used promoters, other suitable microbial promoters may be used as well.
Preferred promoters for practicing this inventan are those that can be tightly regulated such that expression of the fusan gene can be controlled. It is believed that the problem that went unrecognized in the prior art was that display of multiple copies of the fusion protein on the surface of the phagemid particle lead to multipoint attachment of the phagemid with the target. It is believed this effect, referred to as the 'chelate effect', results in selection of false 'high affinity' polypeptides when m~dtiple copies of the fusion protein are displayed on the phagemid particle in dose proximity to one another so that the target was'chelated'. When multipoint attachment occurs, the effective or apparent Kd may be as high as the product of the individual Kds for each copy of the displayed fusan protein. This effect may be the reason Cwirla and coworkers supra were unable to separate moderate affinity peptides from higher affinity peptides.
It has been discovered that by tightly regulating expressan of the fusion protein so that ra more than a minor amount, i.e. fewer than about 1 °~, of the phagemid particles contain multiple copies of the fusion protein the 'chelate effect' is overcome allowing proper selection of high affinity polypeptides. Thus, depending on the 3 0 promoter, culturing conditions of the fast are adjusted to maximize the number of phagemid particles containing a single copy of the fusion protein and mirimize the number of phagemid particles containing multiple copies of the tusion protein.
Preferred promoters used to practice this invention are the l~ Z promoter and the ~ A promoter.
The !~ Z promoter is regulated by the lac repressor protein j~ f, and thus transcription of the fusion gene can be controlled by manipulation of the level of the lac repressor protein. By way of illustration, the phagemid containing the )~ Z promotor is grown in a ceu strain that contains a copy of the )~ f repressor gene, a repressor for the l~ Z promotor. Exemplary cell strains containing the )~ f gene include JM 101 and XL1-blue. In the alternative, the host cell can be cotransfected with a plasmid containing Moth the repressor !~ f and the I3~ Z promotor.
Occasionally both of the above techniques are used simultaneously, that is, phagmide particles containing the l~ Z
WO 92/09690 ~ ~ ,~ ~ ~ PfT/fJS91/09133 promoter are grown in oeti strains containing the ~ i gene and the ceu strains are catransfected with a plasmid containing both tt~e ~ Z and )~ i genes. Normally when one wishes to express a gene, to the transfected host above one would ~d an inducer such as isopropylthiogalacboside (IPTG). In the present invention however, this step is omitted to (a) minimize the expression of the gene III fusion protein thereby minimizing the copy number (i.e. the number of gene III iusans per phagemid number) and to (b) prevent poor or improper packaging of the phagemid caused by induoers such as IPTG even at bw corxentrations. Typcally, when no inducer is ceded, the number of fusion proteins per phagemid partide is about 0.1 (number of bulk fusion proteinslnumber of phagemid partides). The most preferred promoter used to practice this invention is p~
A. This promoter is believed to be regulated by the level of inorganic phosphate in the cell where the phosphate acts to down-regulate the activity of the promoter. Thus, by depleting cells of phosphate, the activity of the promoter can be increased. The desired result is achieved by grovhng cells in a phosphate enriched medium such as 2n or LB thereby controlling the expression of the gene III fusion.
One other useful component of vectors used to practice tNs invention is a signal sequence. This sequence is typically located immediately 5' to the gene encoding the fusion protein, and will thus be transcribed at the amino terminus of the fusan protein. However, in certain cases, the signal sequence has been demonstrated to be located at positions other 5' to the gene encoding the protein to be secreted. This sequence targets the protein to which it is attad~ed across the imer membrane of the bacterial cell. The DNA
encoding the signal sequer~e may be obtained as a restriction erxionucease fragment from any gene encoding a protein that has a signal sequence.
Suitable prokaryotic sisal sequences may be obtained from genes encoding, for example, Lama or OmpF (along et al, t~, 68:193 [1983j), MaIE, PhoA and other genes. A preferred prokaryotic signal sequence for practidng this invention is the E. cbli heat-stale enterotoxin II (STII) signal sequence as described by Chang ef a!. , ~g,pg, 55: 189 [ 1987j.
Another useful component of the vectors used to practice this invention is phenotypic selection genes.
Typical phenotypic selection genes are chose encoding proteins chat confer antibiotic resistance upon the host cell.
By way of illustration, the ampicillin resistance gene (~), and the tetracydine resistance gene (t~ are readily employed for this purpose.
Construction of suitable vectors comprising the aforementioned components as weU as the gene encoding the desired polypeptide (gene 1 ) are prepared using standard recombinant DNA
procedures as described in Samtxook et al. supra. Isolated DNA fragments to be combined m form the vector are cleaved, tailored, and 3 0 ligated together in a spedfic order and orientation to generate the desired vector.
The ONA is deaved using the appropria~ restriction enzyme or enzymes in a suitable buffer. In general, about 0.2-1 ~g of plasmid or DNA fragments is used with about 1-2 units of the appropriate restriction enzyme in about 20 p.1 of buffer solution. Appropriate buffers, DNA concentrations, and incubation times and temperatures are sped5ed by the manufacturers of the restriction enzymes.
Generally, incubation times of about one or iwo hours at 3TC are adequate, although several enzymes require higher temperatures. After incubation, the enzymes and other contaminants are removed by extraction of the digestion solution with a mixture of phenol and chloroform, and the DNA is recovered from the aqueous fraction by precipitation with ethanol.
To ligate the DNA fragments together to form a functional vector, the ends of the DNA fragments must be compatible with each other. In some cases, the ends will be directly compatible after endonudease WO 92/09690 ~ ~ ~ ~ ~ j ~~ PCT/US91/09133 digestion. However, it may be necessary to first convert the sticky ends commoNy produced by endonudease digestion to blunt ends to make them compatible for Ggation. To blunt the ends, the DNA is treated in a suitable buffer for at least 15 minutes at 15'C with 10 units of of the Klenow fragment of DNA polymerise I (Klenow) in the presence of the four deoxynudeotide triphosphates. The DNA is then purified by phenol-chloroform extraction and ethanol predptatan.
The deaved DNA fragments may be size-separated and selected using DNA gel electrophoresis. The DNA may be electrophoresed through either an agarose or a polyacrylamide matrix. The selection of the matrix will depend on the size of the DNA fragments to be separated. After electrophoresis, the DNA is extracted from the matrix by electroelutan, or, if low-melting agarose has been used as the matrix, by melting the agarose and extracting the DNA from it, as described in sections 6.30-6.33 of Sambrook et aL, supra.
The DNA fragments that are to be ligated together (previously digested with the appropriate restriction enzymes such that the ends of each fragment to be ligated are compatible) are put in solution in about equimolar amounts. The solution will also contain ATP, ligase buffer and a ligase such as T4 DNA ligase at about 10 units per 0.5 ug of DNA. If the DNA fragment is to be ligated into a vector, the vector is at first linearized by , cutting with the appropriate restriction endonudease(s). The linearized vector is then treated with alkaline phosphatase or calf intestinal phosphatase. The phosphatasing prevents self-ligation of the vector during the Iigation step.
After ligation, the vector with the foreign gene now inserted is transformed into a suitable host cell.
Prokaryotes are the preferred host cells for this invention. Suitable prokaryotic host cells inducts E. colt strain JM101, E. colt K12 strain 294 (ATCC number 31,446), E. colt strain W3110 (ATCC
number 27,325), E. colt X1776 (ATCC number 31,537), E. colt XL-1 Blue (stratagene), and E colt B;
however many other strains of E.
colt, such as H8101, NM522, NM538, NM539, and many other species and genera of prokaryotes may be used as well. In addition to the E. aoli strains listed above, badlli such as ~(~j~, other enterobacteriaceae such as m a and various p,~speaes may all be used as hosts.
Transformation of prokaryotic cells is readily accomplished using the caldum chloride method as described in section 1.82 of Sambrook et al., supra. Alternatively, electroporation (Neumann etaL, EMBO J..J..
1:841 [1982J) may be used to transform these cells. The transformed cells are selected by growth on an antit~iotic, commonly tetracydine (tet) or ampidllin (amp), to which they are rendered resistant due to the presence of tet and/or amp resistance genes on the vector.
After selection of the transformed cells, these cells are grown in culture and the plasmid DNA (or other vector with the foreign gene inserted) is then isolated. Plasmid DNA can be isolated using methods known in the art. Two suitable methods are the small scale preparation of DNA and the large-scale preparation of DNA as described in sections 1.25-1.33 of Sambrook et al., supra. The isolated DNA
can be purified by methods known in the art such as that described in section 1.40 of Sambrook etal., supra. This purified plasmid DNA is then analyzed by restriction mapping andlor DNA sequendng. DNA sequendng is generally pertormed by either the method of Messing et al. ~gg3" 9:309 [1981 J or by the method of Maxim et aL
~Qg~pty~j" 65:
ass [ls6oJ.
!V.
This invention contemplates fusing the gene erxlosing the desired polypeptide (gene t ) to a second gene (gene 2) such that a fusion protein is generated during transcription. Gene 2 is typically a coat protein gene of a phage, and preferably it is the phage M13 gene III coat protein, or a fragment thereof. Fusan of genes t and 2 may 5 be accomplished by inserting gene 2 inb a parGaa~aar site on a plasmid that contains gene 1, or by inserting gene 1 into a particular site on a plasmid that contains gene 2.
Insertion of a gene into a ptasmid requires that the plasmid be cut at the precise locatan that the gene is to be inserted. Thus, there must be a restriction erxionudease sibs at this bcation (preferably a unique site such that the plasmid will only be cut at a single location during restriction endorx~dease digestion). The plasmid is 10 digested, phosphatased, and purified as described above. The gene is then inserted into this linearized plasmid by ligatirg the two DNAs together. Ligatan can be accomplished if the ends of the plasmid are compatible with the ends of the gene to be inserted. If the restriction enzymes are used to cut the plasmid and isolate the gene to be inserted create blunt ends or compatible sticky ends, the DNAs can be ligated together directly using a ligase such as bacteriophage T4 DNA ligase and irxubating the mixture at 16'C for t ~4 hours in the presence of ATP
15 and Ngase buffer as described in section 1.68 of Sambrook et aL, ~. If the ends are rat compatible, they must first be made Bunt by using the Klenow fragment of DNA polymerase I or bacteriophage T4 DNA polymerase, both of which require the four deoxyribonudeotide triphosphates to fill-in overhanging single-stranded ends of the digested DNA Alternatively, the ends may be Bunted using a nuclease such as nuclease S1 a mung-bean rn~clease, both of which function by cutting back the overtranging single strands of DNA. The DNA is then 2 0 religated using a ligase as described above. In some cases, it may not be possible 6o Bunt the ends of the gene to be inserted, as the reading frame of the coding region will be altered. To overcome this problem, o6gonuGeotide linkers may be used. The linkers serve as a bridge to connect the plasmid m the gene to be inserted. These linkers can be made synthetically as double stranded or single stranded DNA using standard methods. The linkers have one end that is compatible with the ends of the gene b be inserted; the IiNcers are first ligated to this gene using Igation methods described above. The other end of the linkers is desgned to be compatible with the plasmid for ligation. In designing the linkers, care must be taken to not destroy the reading frame of the gene to be inserted or the reading frame of the gene contained on the plasmid. In some cases, it may be necessary to design the linkers such that they code for part of an amino acrd, or such that they code fa one or more amino aads.
Between gene 1 and gene 2, DNA encoding a termination colon may be inserted, such termination colons are UAG( amber), UAA (odder) and UGA (opal). (Microbiology, Davis et al.
Harper l~ Row, New York,1980, pages 237, 245-47 and 274). The termination colon expressed in a wild type host cell results in the synthesis of the gene t protein product without the gene 2 protein attached. However, growth in a suppressor host cell results in the synthesis of detectable quantities of fused protein. Such suppressor host cells contain a tRNA
modified to insert an amino acrd in the termination colon position of the mRNA
thereby resulting in production of detectible amounts of the fusion protein. Such suppressor host cells are well known and described, such as E.coli suppressor strain (Bullock et al., BioTechnioues 5, 376-379 [i987)). Any acceptable method may be used to place such a termination colon into the mRNA erxxxiing the fusion polypeptide.
The suppressible colon may be inserted between the first gene erxoding a polypeptide, and a second gene encoding at least a portion of a phage coat protein. Alternatively, the suppressible termination colon may be WO 92/09690 PCl'/US91/09133 2~~~5~~ ,s inserted adjacent to the fusion site by replacing the last amino acid triplet in the polypeptide or the first amino acrd in the phage coat protein. When the phagemid containing ttre suppressible colon is grown in a suppressor host cell, it results in the detectable production of a tusan polypeptide containing the polypeptide and the coat protein. When the phagemid is grown in a non-suppressor host cell, the polypeptide is synthesized substantially without fusion to the phage coat protein due to termination at the inserted suppressible triplet encoding UAG, UAA, or UGA. In the non-suppressor cell the polypeptide is synthesized and secreted from the host cell due to the absence of the fused phage coat protein which otherwise anchored it to the host cell.
V.
Gene 1, erxoding the desired poiypeptide, may be altered at one or more selected colons. An alteration , 0 is defined as a substitution, deletion, or insertion of one or more colons in the gene encoding the polypeptide that results in a change in the amino acid sequence of the polypeptide as compared with the unaltered or native sequence of the same polypeptide. Preferably, the alterations will be by substitution of at least one amino acid with any other amino acid in one a more regions of the molecule. The alterations may be produced be a variety of methods knoHm in the art. These methods include but are not limited to oligonudeotide-mediated mutagenesis and cassette mutagerresis.
81~
Oligonucleotide -mediated mutagenesis is preferred method for preparing substitution, deletion, and insertion variants of gene 1. This techr~que is weA knorm in the art as described by Zoller et al. Nucleic Aads Res.
IQ: 6487504 [1987]. Briefly, gene 1 is altered by hybridizing an oligonuGeotide encoding the desired mutation to a DNA template, where the 0emplate is the single-stranded form of the plasmid containing the unaltered or native DNA sequence of gene t. After hybridization, a DNA polymerise is used to synthesize an entire second complementary strand of the template will thus incorporate the oligonudeotide primer, and will code for the selected alteration in gene 1.
Generally, oligonuGeotides of at least 25 nucleotides in length are used. An optimal oligonuGeotide will have 12 to 15 nuGeotides that are completely complementary to the template on either side of the nudeotide(s) coding for the mutation. This ensures that the oligonuGeotide will hybridize properly to the single-stranded DNA
template molecule. The digonudeotides are readily synthesized using techniques IQrowrr in the art such as chat described by Crea et al. Proc. Nat,. Acid. Sa. USA 75: 5765 [1978].
The DNA template can only be generated by those vectors that are either derived from bacteriophage M13 vectors (the commercially available M13mp18 and M13mp19 vectors are suitable), or those vectors that contain a single-stranded phage orgin of replication as described by Viera et aL x,",53: 3 [1987].
Thus, the DNA that is to be mutated must be inserted into one of these vectors in order to generate single-stranded template. Production of the single-stranded template is described in sections 4.21-4.41 of Sambrook et al., supra.
To alter the native DNA sequence, the oligonudeotide is hybridized to the single stranded template under suitable hybridization conditions. A DNA polymerizing enzyme, usually the Klenow fragment of DNA
polymerise I, is then added to synthesize the complementary strand of the template using the oligonucleotide as a primer for synthesis. A heteroduplex molecule is thus formed such that one strand of DNA encodes the mutated form of gene 1, and the other strand (the original template) encodes the native, unaltered sequence of gene t .
WO 92/09690 ~ ~ !~ ~ PCT/US91/09133 This heterodupex molecule is then transformed into a suitable host cell, usually a prokaryote such as E. Coil JM101. After growing the cells, they are plated onto agarose plates and screened using the oGgonudeotide primer radiolabelled with 32-Phosphate to identify the bacterial colonies that contain the mutated DNA.
The method described immediately above may be modified such U~at a homoduplex molecule is created wherein both strands of the plasmid contain the mutation(s). The modifications are as follows: The single-stranded oGgonucleotide is ar~aied to the single-stranded template as described above. A mixture of three deoxyribonudeotides, deoxyriboadenosine (dATP), deoxyriboguanosine (dGTP), and deoxyribohymidine (dTTP), is combined with a modified thio~deoxyribocytosine called dCTP-(aS) (which can be obtained from Amersham).
This mixture is added to the template-o~gonudeotide complex. Upon addition of DNA polymerise to this mixture, a strand of DNA identical bo the template except for the mutated bases is generated. h addition, this new strand of DNA will contain dCTP-(aS) instead of dCTP, which serves to protect it from restriction endonudease digestion. After the template strand of the double-stranded heteroduplex is nicked with an appropriate restriction enzyme, the template strand can be digested with Exolll nudease or another appropriate nuGease past the region that contains the sites) to be mutagenized. The reaction is then stopped to leave a molecule that is only partially single-stranded. A complete double-stranded DNA homoduplex is then formed using DNA
polymerise in the presence of all lour deoxyribonudeotide triphosphates, ATP, and DNA Ggase. This homoduplex molecule can then be transformed hto a suitade host cell such as E. colt JM101, as described above:
Mutants witty more than one amino acrd to be substituted may be generated in one of several ways. 1f the amino aids are boated dose together h the pdypeptide chain, they may be mutated simultaneously u~rg one 2 0 oligonudeotide that codes for all of the desired amino acrd substitutions.
If, however, the amino acids are located some distance from each other (separated by more than about ten amino acids), it is more difficult to generate a single digonudeotide that encodes all of the desired changes. Instead, one of two alternative methods may be employed.
In the first method, a separate oligonudeotide is generated for each amino add to be substituted. The oligonudeotides are then amealed to the single-strarxJed template DNA
simultaneously, and the second strand of DNA that is synthesized from Use template wil encode all of the desired amino add substitutions. The alternative method involves two or more rounds of mutagenesis to produce the desired mutant. The first round is as described for the single mutants: wild-type DNA is used for the template, an oliganudeotide encoding the first desired amino acid substitutions) is annealed to this template, and the heteroduplex DNA molecule is then generated. The second round of mutagenesis utilizes the mutated DNA produced in the first round of mutagenesis as the template. Thus, this template already contains one or more mutations. The oligonucleotide encoding the additional desired amino acid substitutions) is then annealed to this template, and the resulting strand of DNA now encodes mutations from both the first and second rounds of mutagenesis. This resultant DNA
can be used as a template in a third round of mutagenesis, and so on.
B.
This method is also a preferred method for preparing substitution, delet'ron, and insertan variants of gene 1. The method is based on that described by Wells et at. ~,, 34:315 [1985].. The starting material is the plasmid (or other vector) comprising gene 1, the gene to be mutated. The codon(s) in gene 1 to be mutated are identified. There must be a unique restriction endorwdease site on each side of the identified mutation site(s). If ~' '~" f a 18 ~~.~~..~ a.~~~
no such restriction sites exist, they may be generated using the above~iesaibed olgonudeotide-mediated mutagenesis method to introduce them at appropriate locations in gene 1. After the restriction sites have been introduced into the plasmid, the plasmid is cut at these sires to linearize it. A double-stranded oligonudeotide encoding the sequence of the DNA between the restriction sites but containing the desired mutations) is synthesized using standard prooe~res. The two strands are synthesized separately and then hybridized together using standard techniques. This double-stranded oligonudeotide is referred to as the cassette. This cassette is designed to have 3' and 5' ends that are compatide with the ends of the linearized plasmid, such that it can be directly ligated 1o the plasmid. This ptasmid now contains the muhated DNA sequence of gene 1.
VI.
In an altemadve embodiment, this invention contemplates production of variants of a desired protein containing one or more subunits. Each subur~t is typically encoded by separate gene. F~ch gene encoding each subunit can be obtained by methods knovm in the art (see, for example, Section II). In some instances, it may be necessary to obtain the gene encoding the various subunits using separate techniques selected from any of the methods described in Section II.
When constmcting a replicable expression vector where the protein of interest contains more than one subunit, all subunits can be regulated by the same promoter, typically located 5' to the DNA encoding the subunits, or each may be regulated by separate promoter suitably oriented in the vector so that each promoter is operably linked to the DNA it is intended to regulate . Selection of promoters is carried out as described in Section III
above.
In constructing a repNcade expression vector aoMaining DNA encoding the protein of interest having multiple subunits, the reader is referred to Figure 10 where, by way of illustration, a vector is diagrammed showing DNA encoding each subunit of an antibody fragment. This figure shows that, generally, one of the subunits of the protein of interest will be fused to a phage coat protein such as M13 gene III. This gene fusion generally will contain its own sgnal sequence. A separate gene encodes the other subunit or suburits, and it is 2 5 apparent that each subur~it generally has its own signal sequerxe. Fgure 10 also shows that a single promoter can regulate the expression of both subunits. Alternatively, each subunit may be independently regulated by a different promoter. The protein of interest subunit-phage coat protein fusion construct can be made as described in Section IV above.
When constructing a famtly of variants of the desired multi-subunit protein, DNA encoding each subunit 3 0 in the vector may mutated in one or more positions in each suburut. When multi-subunit antibody variants are constructed, preferred sites of mutagenesis correspond to colons encoding amino acid residues located in the complementarily-determining regions (CDR) of either the light chain, the heavy chain, or both chains. The CDRs are commonly referred to as the hypervariable regrons. Methods for mutagenizing DNA encoding each subunit of the protein of interest are conducted essentially as described in Section V
above.
VII.
Target proteins, such as receptors, may be isolated from natural sources or prepared by recomt~inant methods by procedures known in the art. By way of illustration, glycoprotein hormone receptors may be prepared by the technique described by McFarland et al., 245:494-499 [1989J, norglycosylated forms expressed WO 92/09690 ~ ~ ~ ~, ~ ~ PCT/US91 /09I 33 in E. colt are described by Fuh et al. J. Biol. Chem 265:3111-3115 [1990]
Otter receptors can be prepared by standard methods.
The purified target protein may be attached to a suitable matrix such as agarose beads, acrylamide beads, glass beads, cellulose, various acxytic copdymers, hydroxylalkyl methaaylate gels, polyaaylic and polymethacrylic copolymers, nylon, neutral and ionic carriers, and the ike.
Attachment of the target protein to the matrix may be accomplished by methods described in ~p~p~, 44 (1976j, or by other means known in the art.
After attachment of the target protein to the matrix, the immobilized target is contacted with the library of phagemid particles under conditions suitable for binding of at least a portan of tf~e phagemid particles with the immobilized target. Nortnaliy, the conditions, including pH, ionic strength, temperature and the like will mimic physiological conditions.
Bound phagemid particles ('binders') having high affinity br the immobilized target are separated from those having a low affinity (and thus do not hind to the target) by washing. Binders may be dissociated from the immot~ilized target by a variety of methods. These methods include competitive dissodation using the wild-type ligand, altering pH arKilor ionic strength, and methods known in the art.
Suitable host cells are infected with the hinders and helper phage, and the host cells are cultured under conditans stable for amplification of the phagemid particles. The phagemid particles are then collected and the selection process is repeated one or more times unto hinders having the desired affinity for the target molecule are selected.
Optionally the library of phagemid particles may be sequentially contacted with more than one immolHlized target to improve selectivity for a particular target. For example, it is often the case that a ligand such as hGH has more than one r~at~al receptor. h the case of hGH, both the growth hormone receptor and tte prolaetin receptor bind the hGH ligand. ft may be desirable to improve the selectivity of hGH for the growth hormone receptor over the prolactin receptor. This can be achieved by ffrst contacting the library of phagemid particles with immot~ilized prolactin receptor, eluting those with a tow affinity (i.e. lower than wild type hGH) for tt~e prolactin receptor and then contacting the bw affinity prolactin 'binders' or non-hinders with the immobilized growth hormone receptor, and selecting for high affinity growth hormone receptor binders. In this case an hGH mutant having a lower affinity for the prolactin receptor would have therapeutic utility even if the affhity for the growth hormone receptor were somewhat lower than that of wild type hGH. This same strategy may be employed to improve selectivity of a particular hormone or protein for its primary function receptor over its clearance receptor.
In another embodiment of tfys invention, an improved substrate amino acid sequence can be obtained.
These may be useful for making better 'cut sites' for protein linkers, or for better protease substratesl~r~ihitors. h this embodiment, an immobitizable molea~e (e.g. hGH-receptor, biotin-avidin, or one capable of covalent linkage with a matrix) is fused to gene III through a linker. The linker will preferably be from 3 to 10 amino aads h length and will act as a substrate for a protease. A
phagemid wiU be constructed as described above where the DNA encoding the linker region is randomly mutated to produce a randomized library of phagemid particles with different amino acid sequences at the linking site. The library of phagemid particles are then immobilized on a matrix and exposed to a desired protease. Phagemid particles having preferred or better substrate amira acld ~ In the liner region kx the desired protease wAi be ekrted, fret pradudrtp an enriched pod of P~9em'd P~des er~adl~p prererred linkers. these phapemid tides are then cycled several mare limes lo t~ epos of particles an~n9 tense seqtrsnve(s) (~ epos Xlll and XIV~.
lltlt. ~Ge.YHdiIO~.A~d~, The domed gene for trGH rtes teen expressed ~ a seaetad tons in Fteals (G~4, C. rb, et al., (1987) hem ~5..i 89) and as oNn and amino add sequsnpa h~ been reported (Goeddel. er ~. [1 s79) ~Q.1.
544; Gray et af., l19&5] ~33~ 247?. The present inversion desaibss navel hGH
variants Prad~d u~n9 die p~~;d ~~pn methods. Human growth hormone verierss ~ fak p'°~~ns 10,14,18, 21, 187,171,172. l 74, 175,176,178 and 179 have been drd~ T~~ ~~rD trigher txndlng affinities are 10 deSCa'Ib6d ~ Tables VII, Xllt irld KiV. The amino acid rl~erl~t~ ~r deSCl~irlp 1119 Var~r~ is flow.
Growth honronB variants may be admlrtlstsred aM ~ ~ ~ s~ ~ "~"~r t~'"~
h°n"one. The growth hormone variants of the present invention may be expressed in any recomt~inanl system which is capable of expressing dative or met hGH.
T~~utic i4rmulafions of hGH for therapeutic admiryistration are prepared for storage by mixing 15 hGH having the desired degr~ of purity with opnonal physialogicatly atxeptable Carriers, exGipients, or stabilizers (13~71nnlon's Pita(m$~C~,L$C1~ ~~~ ~~ A.. ~d., (19801., in ~ form of tyoptw6ied cake or a4uevug ~lutions. Acceptable carriers, exoit>ienls or stabilizers era nontoxic bo reripiants at the dosages and C9n0enbailOns em~l6yEd, and irSClllde bllflta5 ~ 88 pfb"~, a~dte, arid DI~IBr Organic acids; ~tibbxid3nt5 inGuding asoabic sad; kyw mo'lsatlar weight pass than about 10 residues) poiypeptides; proteins. s~h as serum 2 o altxJmin. gelatin. or Immmogk~Gns: hydrnph~ic polymers auc+r as t~PYmolndone; amino atdds s~d~ as glycir~e, glutamine, asparagina, arginine, or lysine: monosaccharides, dis~rides, and other cart#tydrates including glucose. rnamose, or dexuins; chelating agents such as EDTA; divaler>t metal ions such as zinc, cobalt or copper:
sugar altohofs such as rnarrstol or sorbital; salt~tortning txsunlerions such as sodium; anchor norikmic suriacranls such as Tween; Pluronies~r po>yefhytene gtycot (isEG). Fortnulatior>s of the present invention may additionally 25 contain a phartnaaeuticallY aooeptable.butter, amino add, bulking agent arrdror non-ionic siufactant. These include, for example, buffers. tdieiating agents, antibxidarlLS, preservatives, cosotvent5, arid the like; spetxtic examples 9t these could include, trimethylamakte salts ('iris butter"), and dtsot8txn sdetate. Ttte phapemids of the present Invention may ba used to produce qttantitiefi bf the htahl verlar115 free of the phage pr018m. To expra35s ttGH
variants free of the gene III portion Of tt~a fusion, pS0643 and derivatives can simply be grown in a rtorr 30 suppressor strain such as i6C9. In ttris case, the amber colon (TAG) leads to tetrnlr>ation of iralnslation, which yields tree homtlxte, vntllout the need for an independent DNA consinxtion.
The frGH variant is setxeted from the host and may be isdated from the collars med'nim.
One or mbre of the e(ght hGH amino adds F10, M14, HiB, ill. Rt87, D171, T175 and 1179 may be raped by any amino tad other than the one found in that position (n r~turs~y rig hGH
as ~dicated. Thereiore,1, 2, 35 3, 4, 5, ti, 7, a a0 t3 of the indicated amino acids, F10, M14, H18, H21, Rtfi7,17171, T175 and 1179, may he replaced by any or the other 19 amino acids out of the 20 amino acids listed below. tn a preferred embodunerlt, as eight listed amino acids are replaced by another amino aid. The most preferred eight amino acids to be substituted are indicated in Table XIV in Example XII.
*trarlemark 2, An,ho add nomr~cla~re.
Ala (A) Arg (R) Asn (N) Asp (D) Cys (C) Gln (0) Glu (E) p Giy (G) His (H) Ile (I) Leu (L) Lys (K) Met (M) Phe (F) Pro (P) Ser (S) Thr (T) Trp (W) Tyr (Y) Val (V) The one letter hGH variant nomendadxe first gives the hGH amino acrd deleted, for example glutamate 179; then the amino acid inserted; for example, serine; resulting in (E1795S).
EXAMPLES
Without further desaiptan, it is believed that one of ordinary skill in the art can, using the preceding description and illustrative examples, make and utilize the present invention to the fullest extent. The follov~ng working examples therefore speafically point out preferred embodiments of the present invention, and are not to 3 0 be construed as limiting in any way of the remainder of the disclosure.
EXAMPLE I
Plasrrdd Cor~uciJons and Preparation o1 Mali-phagerr~ld Parfides The plasmid phGH-Ml3glll (Fig.1 ), was oonstnxted from M13K077 and the hGH
producing plasmid, pB0473 (Cunningham, B. C., et aL , ~jg~, 243:1330-1336, [1989]). A synthetic oligonucleotide 5'-AGC
TGT-GGC-TTC-GGG'CCC-TTA-GCA-TTT-AAT-GCG-GTA-3' was used to introduce a unique Apal restriction sib (underlined) into p80473 after the final Phel9t colon of hGH.
The oligonudeotide 5'-TTC-ACA-AAC-GAA-GGGCCC-CTA-ATT-AAA-GCC-AGA-3' was used to introduce a unique Apal restriction site (underlined), and a GIut97-to-amber stop oodon (bold lettering) into M13K07 gene III. The oligonuGeotide 5'-CAA-TAA-TAA-CGG-~'C~ T-AGC-CAA-AAG-AAC-TGG-3' introduces a unique Nhel site (underlined) after the 3' end of the gene III coding sequence. The resulting 650 base pair (bp) Apal-Nhel fragment from the doubly mutated M13K07 gene III was doped into the large Apal-Nhel fragment of pB0473 to create the plasmid, pS0132. This fuses the carboxyl terminus of hGH (Phe191 ) to the Pro198 residue of the gene III protein with the insertan of a glydne residue errcoded from the Apal site and places the fusion protein under control of the E. coG
alkaline phosphatase (ptaA) promoter and stll secretion signal sequence (Chang, C. N., et al. , ~gpg, 55:189-196, [1987)). For indudble expression of the fusion protein in rich media, we replaced the phoA promoter with the lac promoter and operabr. A 138 by EcoRl-Xbal fragment containing the lac promoter, operator, and Cap binding site was produced by PCR of plasmid pUC119 using the oligonudeotides 5'-CACGACAGAATTCCCGACTGGAAA-3' and 5'-CTGTT TCTAGAGTGAAATTGTTA-3' that flank the desired lac sequences and introduce the EcoRl and Xbal restriction sites (underlined).
Ttws lac fragment was gel pur'rfied and ligated into the large EcbRl-X6al fragment of pS0132 to create the plasmid, phGH-Ml3glll. The sequences of all taibred DNA junctions were verified by the dideoxy sequerxe method (Sanger, F., et aL Proc. Nail. Acad.
~j,~,~,g, 74:5463-5467, [1977)). The R64A variant hGH phagemid was constructed as follows: the Nsil-Bglll mutated fragment of hGH (Cunninghamet al. supra ) erxading the Arg64 to Ala substitution (R64A) (Cunningham, B. C., Wells, J. A., ~jgp~, 244:1081-1085, [1989]) was Boned between the corresponding restriction sites in the phGH-Ml3glll plasmid (Fig. 1) to replace the wild-type hGH sequence. The R64A hGH
phagemid particles were propagated and titered as described bebw for the wild-type hGH-phagemid.
Plasmids were transformed into a male strain of E. cali (JM101 ) and selected on carbenidllin plates. A
single transformant was grown in 2 ml 2n medium for 4 h at 3TC and infected with 50 W of Mt3K07 helper phage. The infected culture was diluted into 30 ml 2YT, grown overnight, and phagemid particles were harvested by predpitation with polyethylene glycol (Vierra, J., Messing, J. ,153:3-11, (1987]).
Typical phagemid particle titers ranged from 2 to 5 x 1011 cfu/ml. The particles were purified to homogeneity by CsCI density oentrrfugation (Day, L.A. J. Mol. Biol., 3965-277, (1969]) to remove any fusion protein not attached to virions.
hsrnnOd>e~al Analyses Of hGH m the Futon Phape Rabbit polydonal antibodies to hGH were purified with protein A, and coated onto microtiter plates (Nuns) at a corxernration of 2 Irglml in 50 mM sodium carbonate buffer (pH 10) at 4'C for 16-20 hours. After washing in PBS containing 0.05% Tween 20, hGH or hGH-phagemid particles were senialty diluted from 2.0 -0.002 nM in buffer A (50 mM Tris (pH 7.5), 50 mM NaCI, 2 mM EDTA, 5 mglml bovine serum albumin, and 0.05%
Tween 20). After 2 hours at room temperature (rt), the plates were washed well and the indicated Mab (Cunninghamet al. supra ) was added at 1 Nglml in buffer A for 2 hours at rt.
Following washing, horseradish peroxidase conjugated goat anti-mouse IgG antibody was bound at rt for 1 hour.
After a final wash, the peroxidase activity was assayed with the substrate, o~phenylenediamine.
3 5 FxAMPLE IA
Coupling of the hGH Binding Rolein bo Pdyaaylamlde Beads and Binding Eryichments Oxirane polyacrylamide beads (Sigma) were conjugated to the purified extracellular domain of the hGH
receptor (hGHbp) (Fuh, G., etal., J. Biol. Chem., 265:3111-3115 (1990)) containing an extra cysteine residue introduced by site-directed mutagenesis at positron 237 that does not affect binding of hGH (J. Wells, WO 92/09690 ~ ~ ~~ PCT/US91 /09133 unpublished). The hGHbp was corrugated as recommended by the supplier to a level of 1.7 pmol hGHbplmg dry oxirane bead, as measured by binding of [tag hGH b the resin. Subsequendy, any unreacted oxirane groups were blocked with BSA and Tris. As a control for non-specific binding of phagemid particles, BSA was similarly coupled to the beads. Buffer for adsorption and washing contained 10 mM
Tris~HCl (pH 7.5),1 mM EDTA, 50 mM
NaG,1 mglml BSA, and 0.0296 Tween 20. Elution buffers contained wash buffer plus 200 nM hGH or 0.2 M
glyclne (pH 2.1). Parental phage M13K07 was mixed with hGH phagemid particles at a rata of nearly 3000:1 (original mixture) and tumded for 8-12 h with a 5 N.I aliquot (0.2 mg of aaylamide beads) of either absorbent in a 50 p.1 volume at room temperature. The beads were peueted by oen~ifugation and the supemate carefully removed. The beads were resuspended in Z00 W wash buffer and tumbled at room temperatrue for 4 hours (wash t). After a second wash (wash 2), the beads were eluted twice with 200 nM hGH for 6-10 hours each (eluate 1, eluate 2). The final elution was with a glyclne buffer (pH 2.1 ) for 4 hours to remove remaining hGH
phagemid particles (eluate 3). Each fraction was diluted appropriately in 2n media, mixed with fresh JM101, incubated at 3TC for 5 minutes, and plated with 3 ml of 2n soft agar an LB or LB carbeniclllin plates.
iD(AMPLE IV
Cor~nrcUan of hGH.phagemld ParOd~ wlth a Mbct~e of Gene 11 Products The gene III protein is composed of 410 residues divided into two domains that are separated by a flexible linker sequence (Armstrong, J., elal., FEES Lett..135:167-172, [1981)). The amino-terrnir~al domain is required for attachment to the pill of E. cori, while the carboxyl-terminal domain is imbedded in the phage coat and required for proper phage assembly (Crissman, J. W., Smith, G. P., Viroloav.
132:445-455. [1984]). The signal 2 0 sequerxe and amino-terminal domain of gene Ill was replaced with the stll signal and entire hGH gene (Chang et al.
supra) by fusion to residue 198 in the carboxyl-terminal domain of gene III
(Fig.1 ). The hGH~ene III fusion was placed under control of the lac promoterloperator in a plasmid (phGH-M13g111;
Fg. 1) containing the pBR322 ~-lactamase gene and Col Et replication origin, and the phage ft intergenic region. The vector can be easily mair><ained as a small plasmid vector by selection on carber~allin, which avoids relying on a functional gene III fusion for propagation. Alternatively, the plasmid can be ef6clently packaged into virions (called phagemid particles) by infection with helper phage such as M13K07 (Yera et al.. supra ) which avoids problems of phage assembly.
Phagemid infectivity titers based upon transduction to carbeniclllin resistance in this system varied from 2-5 x 1011colony forming units (cfu~ml. The titer of the M13K07 helper phage in these phagemid stocks is ~1010 plaque forming units (pfu)Iml.
lAfith this system we confirmed previous studies (Parmiey, Smith supra) that homogeneous expression of large proteins fused to gene III is deleterious to phage production (data not shown). For example, induction of the lac promoter in phGH-Ml3glll by addition of IPTG produced low phagemid titers.
Moreover, phagemid particles produced by oo-infection with M13K07 contairurg an amber mutation in gene III
gave very low phagemid titers (<t010 ciulml). We believed that multiple copies of the gene 111 fusion attached to the phagemid surface could lead to multiple point attachment (the 'chelate effect') of the fusion phage to the immobilized target protein.
Therefore to control the fusion protein copy number we limited transcription of the hGH-gene III fusion by culturing the plasmid in E. colt JM101 (iacl~) which contains a oonstitutively high level of the lac repressor protein.
The E. colt JM101 cultures containing phGH-Ml3glll were best propagated and infected with M13K07 in the absence of the lac operon inducer (IPTG); however, this system is flexible so that co~xpression of other gene III
2~~5~3.3 24 tusan proteins can be balanced. We estimate that about 10°~ of the phagemid particles contain one copy of the hGH gene III fusion protein from the ratio of the amount of hGH per virion (based on hGH immures-reactive material in CsCI gradient purified phagemid). Therefore, the titer of fusion phage displaying the hGH gene III fusion is about 2 - 5 x 1010hn1. This number is much greater than the titer of E. aoli (-108 to l0glml) in the culture from which they are derived. Thus, on average every E. ooli cell produces 10-100 copies of phage decorated with an hGH gene III fusion protein.
EXAMPLE V
Structural Inteprtty of the hGH~ene II Fusion Immunoblot analysis (Fg. 2) of the hGH~ene III phagemid show that hGH aoss-reactive material comigrates with phagemid particles in agarose gels. This indicates that the hGH is tightly assoclated with phagemid particles. The hGH-gene III fusion protein from the phagemid particles runs as a single immuno-stained band showing that there is little degradation of the hGH when it is attached to gene III. Hfild-type gene III protein is dearly present because about 25% of the phagemid particles are infectious.
This is comparable to specific infectivity estimates made for wild-type M13 phage that are similarly purified (by CsCI density gradients) and concentrations estimated by UV absorbance (Smith, G. P. supra and Parmley, Smith supra) Thus, both wild-type gene III and the hGH-gene III fusion proteins are displayed in the phage pool.
It was important to confirm that the tertiary structure of the displayed hGH
was maintained. in order to have confidence that results from Minding selections will translate to the native protein. We used monoclonal antibodies (blabs) to hGH bo evaluate the sUuctural integrity of the displayed hGH gene III fusion protein (Table I).
TABLE L Binding of Eight Different Monoclonal AnfIbodles (Mab"s) to hGH end hGH Phagemld Particles' IC50 (nM) blab hGH hGH-phagemid __________..___________._..._______.________________._______....._________...__ ____ 1 0.4 0.4 2 0.04 0.04 3 0.2 0.2 4 0.1 0.1 5 0.2 >2.0 6 0.07 0.2 7 0.1 0.1 8 0.1 0.1 'Values given represent ~e oorxentrationGH or hGH-phagemid particles (nM) of h to give half-maximal binding to the particular Mab. Standard errors in these measurements are typically at or below 30%
of the reported value.
See Materials and Methods for further details.
The epitopes on hGH for these blabs have been mapped (Cunringham et al..
supra) and Minding for 7 of 8 blabs requires that hGH be properly folded. The IC50 values for all blabs were equivalent to wild-type hGH
except for Mab 5 and 6 . Both blabs 5 and 6 are known to have tHnding determinants near the carboxyl-terminus of hGH which is blocked in the gene III fusion protein. The relative IC50 value for Mabt which reacts with Moth native and denatured hGH is urxhanged oompan;d to the confortnationally sensitive blabs 2-5, 7 and 8. Thus, Mab1 serves as a good internal control for any errors in matching the concentration of the hGH standard to that of the hGH~ene 111 fusion.
WO 92/09690 ~ ~ ~ ~ ~, ~ ~ PCT/US91/09133 EXAMPLE VI
Bin~ng Enrldnferwa on Raoepbor AtANty BeHds Previous workers (Partnley, Smith supra ; Scott, Smith supra; Cwirla et al.
supra; and DeNin et al.
5 supra) have fractionated phage by panning with streptavidin coated polystyrene petri dishes or miaotiter plates.
However, chromatographic systems would allow more efficient fractionation of phagemid particles displaying mutant proteins with different binding affinities. We chose non-porous oxirane beads (Sigma) to avoid trapping of phagemid particles in the chromatographic resin. Furthermore, these beads have a small partite size (1 N.m) to maximize the surface area to mass ratio. The extracellular domain of the hGH
receptor (hGHbp) (Fuh ef al. , 10 supra) containing a free cysteira residue was effiaently coupled m these beads and phagemid particles showed very low non-specific bin ding to beads coupled oMy to bovine serum albumin (Table II).
TABLE II.
15 Specific Binding of Hormone Phage to hGHbp-coated Beads Provides an Enrichment for hGH-phage over M13K07 Phage' Sample Absorbent$ Total pfu Total cfu Ratio (cfu/pfu) Enrichment~
20 Original mixturet 8.3 x 1011 2.9 x 1p8 3.5 x 10'4 (1) Supernatant BSA 7.4 x 1011 2.8 x 108 3.8 x 10'4 1.1 hGHbp 7.6 x 1011 3.3 x 108 4.3 x 10'4 1.2 Wash 1 BSA 1.1 x 1010 6.0 x 106 5.5 x 10'4 1.6 hGHbp 1.9 x 1010 1.7 x 107 8.9 x 10'4 2.5 25 Wash 2 BSA 5.9 x 107 2.8 x 104 4.7 x 10'4 1.3 hGHbp 4.9 x 107 2.7 x 106 5.5 x 10'2 1.6 x 102 Eluate 1 (hGH)BSA t .1 x 106 1.9 x 103 1,7 x 10'3 4.9 hGHbp 1.2 x 106 2.1 x 106 1.8 5.1 x 103 Eluate 2 (hGH)BSA 5.9 x 105 1.2 x 103 2.0 x 10'3 5.7 hGHbp 5.5 x 105 1.3 x 106 2.4 6.9 x 103 Eluate 3 (pH 2.1 )BSA4.6 x 105 2.0 x 103 4.3 x 10'3 12.3 hGHbp 3.8 x 105 4.0 x 106 10.5 3.0 x 104 'The titers of M13K07 and hGH-phagemid particles in each fraction was determined by multiplying the number of plaque forming units (pfu) or carbenicillin resistant colony forming units (cfu) by the dilution factor, respectively. See Example IV for details.
tThe ratio of M13K07 to hGH-phagemid particles was adjusted to 3000:1 in the original mixture.
$Absorbents were conjugated with BSA or hGHbp.
~Enrichments are calculated by dividing the cfu/pfu ratio after each step by cfu/pfu ratio in the original mixture.
In a typical enrichment experiment (Table II), one part of hGH phagemid was mixed with >3,000 parts M13K07 phage. After one cyGe of binding and elution,106 phage were recovered and the ratio of phagemid to M13K07 phage was 2 to 1. Thus, a single binding selection step gave >5000-~Id enrichment. Additional elutions with free hGH or acid treatment to remove remaining phagemids produced even greater enrichments. The enrichments are comparable to those obtained by Smith and coworkers using batch elution from coated polystyrene plates (Smith, G.P. supra and Parmely, Smith sypra ) however much smaller volumes are used on the WO 92/09690 N Q ~ ~ b ~y ~~ PCT/US91/09133 beads (200 W vs. 6 ml). There was almost no enrichment for the hGH phagemid over M13K07 when we used beads linked only to BSA. The slight enrictxnent observed for control beads (-10-fold for pH 2.t elution; Table 2) may result firom trace contaminants of bovine growth hormone t>;n<ling protein present in the BSA linked to the bead. Nevertheless these data show the enrichmer><s for the hGH phage depend ion the presence of the hGHbp on the bead suggesting Minding oxurs by specific interactan between hGH and the hGHbp.
We evaluated the enrichment for wild-type hGH over a weaker bir»dirg variant of the hGH on fusion phagemids to further demonstrate enrichment speafiaty, and to Nnk the reduction in binding affinity for the purffied hormones to ervichment factors after panrwng fusion phagemids. A
fusion phagemid was constmcted with an hGH mutant in which Arg64 was substituted with Ala (R64A). The R64A
variant hormone is about 20-fold reduced in receptor binding affinity compared to hGH (Kd values of 7.t nM
and 0.34 nM, respectively [Cunningham, Wells, supra )). The titers of the R64A hGHt~ene III fusan phagemid were comparable to those of wild-type hGH phagemid. After one round of binding and elution (Table III) the wild-type hGH phagemid was enriched from a mixture of the two phagemids plus M13K07 by 8-fold relative to the phagemid R64A, and 104 relative to M13K07 helper phage.
TABLE WI. hGHbp~ooated Beads Select fa hGH PhaperNds Over a Weaker B4~np hGH Variant tfiapemld Sample enrichment j~ enrichment total phagemid for WT/R64A total phagemid for VIrT/R6~4A
Original mixture 8/20 (1) 8/20 (1) Supernatant ND - 4/10 1.0 Elution 1 (hGH) 7J20 0.8 17120 8.5$
Elution 2 (pH 2.1 ) 11 /20 1.8 21 /27 5.2 'The parent M13K07 phage, wild-type hGH phagemid and R64A pt~agemid particles were mixed at a ratio of 104:0.4.6. Binding selections were carried out using beads linked with BSA
(control beads) or with the hGHbp (hGHbp beads) as described in Table II and the Materials and Methods After each step, plasmid DNA was isolated(&mboim, H. C., Doly, J. , Nucleic Acids Res., T:1513-1523, [1979]) from carbeniallin resistant colonies and analyzed by restriction analysis to determine if it contained the wild-type hGH or the R64A hGH gene III
fusion.
tThe enrichment for wild-type hGH phagemid over R64A mutant was ca~ulated from the rata of hGH phagemid present after each step to that present in the original mixture (8120), divided by the coresponding ratio for R64A phagemids. WT = wild-type; ND = not determined.
$The enrichment for phagemid over total M13K07 parental phage was -104 after this step.
4 0 By displaying a mixture of wild-type gene III and the gene 111 fusion protein on phagemid particles one can assemble and propagate virions that display a large and proper folded protein as a fusion to gene III. The copy number of the gene III fusion protein can be effectively controlled to avoid'chelate effects' yet maintained at high enough levels in the phagemid pool to permit panning of large epitope libraries (>1010). We have shown that hGH
(a 22 kD protein) can be displayed in its native folded torm. Binding selections performed on receptor affinity beads eluted with free hGH, efficiently enriched for wild-type hGH phagemids over a mutant hGH phagemid shown to gave reduced receptor binding affinity. Thus, it is possible to sort phagemid particles whose binding constants are doom in the r~anomolar range.
WO 92/09690 ~ 9 j ~ 3 ~ PCT/US91 /09133 Proteirrprotein and antibody-antigen interactions are dominated by discontinuous epitopes (Janin, J., et al. , J.J. MoLBiol..Biol.. 204:155-164, (1988]; Argos, P., Prot Ena., 2a01-113, (19881; Barlow, D.J.,etaL , ~,, 322:747-748, [1987); and Davies, D.R., et at. , ,(,,Biol. Chem.. 263:10541-10544, [1988)); that is the residues directly involved in binding are dose in tertiary structure but separated by residues not involved in binding. The screening system presented here should albw one to analyze more conveniently protein-receptor interactions and isolate disoont~uous epitopes in proteins with new and high affinity binding properties.
S~don d hGH from a IJbrary Rundomtaed at hGH Colons 172,174,176,178 i 0 Constructi~~n of template A mutant of the hGH~ene III fusion protein was constructed using the method of Kunkel.,et aL fuj~.
Fp~,154, 367-382 [1987]. Template DNA was prepared by growing the plasmid pS0132 (containing the natural hGH gene fused to the carboxy-terminal half of M13 gene III, under control of the alkaline phosphatase promoter) in CJ236 cells with M13-K07 phage added as helper. Single-stranded, uradl-containing DNA was prepared for mutagenesis to introduce (1) a mutation in hGH which would greatly reduce binding to the hGH
binding protein (hGHbp); and (2) a unique restriction site (Kpnl) which could be used for assaying for -- and selecting against - parental background phage. Oligonudeotide-directed mutagenesis was carried out using T7 DNA polymerise and the foNowing digodeoxy-nucleotide:
Gly Thr hGH colon: 178 179 5' -G ACA TTC CTG S-aGT A~.C GTG CAG T-3' < KpnI >
This oligo introduces the Kpnl site as shown, along with mutations (R178G,1179T) ~ hGH. These mutations are predicted to reduce binding of hGH to hGHbp by more than 30-fold. Clones from the mutagenesis were screened by Kpnl digestion and confirmed by dideoxy DNA sequencing. The resulting constrict, b be used as a template for random mutagenesis, was designated pH04t5.
~ ~ggpn Ihel6c~l of hCt( Colons 172,174,176,178 were targeted for random mutagenesis in hGH, again using the method of Kunkel. Single-strarxied template from pH0415 was prepared as above and mutagenesis was carried ouf using 3 0 the following pool of oligos:
hGH colon: 172 174 5'- GC TTC AGG AAG GAC ATG GAC ~ GTC )~ ACA-Ile .5. CTG ~ ATC GTG CAG TGC CGC TCT GTG G-3' As shown, this oligo pool reverts colon 179 to wtid-type (tie), destroys the unique Kpnl site of pH0415, and introduces random colons (NNS, where N= A,G,C, or T and S= G or C) at positions 172,174,176, and 178. Using this colon selection in the context of the above sequence, no additional Kpnl sites can be seated. The choice of the NNS degenerate sequence yields 32 possible colons (inducting one 'stop' colon, and at least one colon for each amino add) at 4 sites, for a total of (32)4= 1,048,576 possible nudeotide sequences (12°~ of which contain at least one stop colon), or (20)4= 160,000 possible polypeptide sequences plus 34,481 prematurely terminated sequences (i.e. sequerxes contair>ing at least one stop colon).
PSg~,~geffon of tnlfjat Ilbrarv WO 92/09690 ~ ~ ~ C~ ~ J ~ PCT/US91/09133 The mutagenesis products were extracted twice with phenolxtrloroform (50:50) and ethanol precpitated with an excess of carrier tRNA 6o avoid adding salt that would confound the subsequent electroporation step. Approximately 50 ng (15 fmols) of DNA was electroporated into WJM101 cells (2.8 x 101 ~
oeIIsImL) in 45 N.L btal volume in a 0.2 an cuvette at a voltage setting of 2.49 kV with a single pulse (time constant = 4.7 msec.).
The ceNs were aNowed to recover 1 hour at 37oC with shaking, then mixed with 25 mL 2YT medium,100 ~glmL carbenicllin, and M13-K07 (multiplicity of infection = 1000). Plating of serial dilutions from this culture onto carbenialGn~onta~ing media indicated that 8.2 x 106 electrotransformarris were obtained. After 10' at 23oC, the culture was incubated overnight (15 hours) at 37oC with shaking.
After ovemght incubation, the cells were pelleted, and double-stranded DNA
(dsDNA), designated pLIB1, was prepared by the alkaline lysis method. The supernatant was spun again to remove any remaining cells, and the phage, designated phage pool ~1, were PEG-precipitated and resuspended in 1 mL STE buffer (10 mM
Tris, pH 7.6, 1 mM EDTA, 50 mM NaCI). Phage titers were measured as colony-formirg units (CFU) for the recombinant phagemid containing hGH~3p gene III fusion (hGH~) plasmid, and plaque-forming units (PFU) for 1 S the M13-K07 helper phage.
1. BINDING: M aliquot of phage pool ~1 (6 x 109 CFU, 6 x 107 PFU) was diluted 4.5-fold in buffer A
(Phosphate-buffered saline, 0.5% BSA, 0.05°~ Tween-20, 0.01°k thimerosal) and mixed with a 5 uL suspension of oxirane-polyacrylamide beads coupled to the hGHbp containing a Ser237 Cys mutation (350 fmols) in a 1.5 mL
silated polypropylene tube. As a control, an equivalent aNquot of phage were mixed in a separate tube with beads that had been coated with BSA only. The phage were allowed to hind to the beads by incubating 3 hours at room temperature (23oC) with slow rotation (approximately 7 RPM). Subsequent steps were carried out with a constant volume of 200~L and at room temperature.
2. WASH: The beads were spun 15 sec., and the supernatant was removed (Sup.1 ). To remove phage/phagemid not specifically bound, the beads were washed twice by resuspending in buffer A, then pelleting.
A final wash consisted of rotating the beads in buffer A for 2 tours.
3. hGH ELUTION: Phagelphagemid Minding weakly to the beads were removed by stepwise elution with hGH. In the first step, the beads were rotated with buffer A containing 2 nM
hGH. After 17 hours , the beads were pelleted and resuspended in buffer A containing 20 nM hGH and rotated for 3 hours, then pelleted. In the 3 0 final hGH wash, the beads were suspended in buffer A containing 200 nM hGH
and rotated for 3 tours then pelleted.
4. GLYCINE ELUTION: To remove the tightest-binding phagemid (i.e. those still bound after the hGH
washes), beads were suspended in Glycne buffer (1 ~Glycine, pH 2.0 with HCI), rotated 2 hours and pelleted.
The supernatant (fraction 'G'; 200~.L) was neutralized by adding 30 Nl. of 1 M
Tris base.
Fraction G eluted from the hGHbp-beads (1 x 106 CFU, 5 x 104 PFU) was not substantially enriched for phagemid over K07 helper phage. We believe this resulted from the fact that K07 phage packaged during propagation of the recombinant phagemid display the hGH-gap fusion.
WO 92/09690 ~ ~ 9 ~ ~ ~ ~ PCT/US91/09133 However, when compared with fraction G eluted from the BSAcoated control beads, the hGHbp-beads yielded 14 times as many CFU's. This reflects the enrichment of tight-binding hGH~displaying phagemid over nonspedfically-binding phagemid.
5. PROPAGATION: M aliquot (4.3 x 105 CFU) of fractan G ekited from the hGHbp-beads was used to infect log-phase WJM101 cells. Transductans were cartied out by mixing 100 N.L fractan G with 1 mL WJM101 cells, incubating 20 min. at 37oC, then adding K07 (multiplidty of infection=1000). Cultures (25 mL 2YT plus carberudllin) were grown as described above and the second pool of phage (Library 1G, for first glydne elution) were prepared as described above.
Phage from library 1 G (Fig. 3) were selected for tlinding to hGHbp beads as described above. Fraction G eluted from hGHbp beads contained 30 times as many CFU's as fir~tion G
eluted from BSA-beads in this selectan. Again, an aliquot of fraction G was propagated in WJM101 cells to yield library 1G2 (indicating that this library had been twice selected by glyane elution). Double-stranded DNA
(pLIB 1 G2) was also prepared from this culture.
To reduce the level of background (Kpnl+) template, an aliquot (about 0.5 p.g) of pLIB 1G2 was digested with Kpnl and electroporated into WJM101 cells. These cells were grown in the presence of K07 (multiplidty of infection= i00) as described for the initial library, and a new phage pool, pLIB 3, was prepared (Fig. 3).
In addition, an aliquot (about 0.5 fig) of dsDNA from the initial library (pLIB1) was digested with Kpnl and electroporated directly into WJM101 cells. Transfortnants were allowed to recover as above, infected with M13-K07, and grown overnight to obtain a new Gtxary of phage, designated phage Library 2 (Fig. 3).
Phagemid binding, elution, and propagation were carried out in successive rounds for phagemid derived from both pLIB 2 and pLIB 3 (Fig. 3) as described above, except that (1) an excess (10-fold over CFU) of p~xified K07 pfrage (not ~sptaying hGH) was added in the bead-binding cocktail, and (2) the hGH stepwise elutions were n;plaoed with txief washings of buffer A alone. Also, in some cases, XL1-Blue cells were used for phagemid propagation.
An additional digestion of dsDNA with Kpnl was carried out on pLIB 2G3 and on pLlB 3G5 before the final round of bead-binding selection (Fg. 3).
Four independently isolated doves from LIB 4G4 and bur indeperxiently isolated doves from LIB 5G6 were sequenced by dideoxy sequendng. All eight of these doves had identical DNA sequences:
hGH colon: 172 174 176 178 5' -AAG GTC TCC ACA TAC CTG AGG ATC-3' Thus, all these encode the same mutant of hGH: (E174S, F176Y). Residue 172 in these Bones is Lys as in wild-type. The colon selected for 172 is also identical to wild-type hGH. This is not surprising since AAG is the only lysine~odon possible from a degenerate 'NNS' colon set. Residue 178-Arg is also the same as wild-type, but here, the colon selected from the library was AAG instead of CGC as is found in wild-type hGH, even though the latter colon is also possible using the'NNS' colon set.
p 30 The multipliclty of infection of K07 infection is an important parameter in the propagation of recomtHnant phagemids. The K07 multiplidty of infection must be high enough to insure that virtually all cells transformed or transfected with phagemid are able to package new phagemid particles. Furthermore, the concentration of wild-type gene III in each cell should be kept high Eo reduce the possibility of multiple hGH-gene III
fusion molecules being displayed on each phagemid particle, thereby reduarg chelate effects in binding. However, if the K07 multiplidty of infection is too high, the packaging of K07 will compete with that of recombinant phagemid. We find that aooeptabfe phagemid yields, vhth oMy 1-10% badc~ound K07 phage, are obtained when the K07 mutGplidty of infection is 100.
Table IV.
Phage Pool moi (K07) Enrichment hGHbpIBSA beads Fraction Kpnl CFUIPFU
LIB 1 1000 ND 14 0.44 LIB 1G 1000 ND 30 0.57 LIB 3 100 ND 1.7 0.26 LIB 3G3 10 ND 8.5 0.18 LIB 3G4 100 460 220 0.13 LIB 2 100 ND 1.7 <0.05 LIB 2G 10 ND 4.1 <0.10 LIB 2G2 100 1000 27 0.18 Phage pools are labelled as shown (Fig. 3). The multiplidty of infection (moi) refers to the multiplidty of K07 infection (PFUlcells) in the propagation of ptragemid. The enrictunent of CFU
over PFU is shown in those cases where purified K07 was added in the binding step. The rata of CFU eluting from hGHbp-beads over CFU eluting from BSA-beads is shown. The fraction of Kpnl-containing template (i.e., pH0415) remaining in the pool was determined by digesting dsDNA with Kpnl plus EcoRl, running the products on a 1°k agarose gel, and laser-scanning a negative of the ethidium bromide-stained DNA.
R~'gp~tor-blndina aftlnHp~r of fhe hormone hGH(E174S. F176Y1 The fact that a single done was isolated from iwo different pathways of selection (Fig. 3) suggested that the double mutant (E174S,F176Y) hinds strongly to hGHbp. To determine the affinity of this mutant of hGH for hGHbp, we constructed this mutant of hGH by site~irected mutagenesis, using a plasmid (pB0720) which contains the wild-type hGH gene as template and the following oligonuGeotide which changes colons 174 and hGH colon: 172 174 176 178 Lys Ser Tyr Arg 5'- ATG GAC AAG GTR ~G ACA T8C CTG CGC ATC GTG -3' The resulting construct, pH0458B, was transformed into E. coli strain 16C9 for expression of the mutant hormone. Scatchard analysis of competitive tHnding of hGH(E174S,F176Y) versus 1251-hGH to hGHbp indicated that the (E174S,F176Y) mutant has a Minding affinity at least 5.0-fold tighter than that of wild-type hGH.
EXAMPLE VUI
SELECTION OF hGH VAFBANTS FROM A
Human growth hormone variants were produced by the method of the present irnention using the phagemid desaibed in figure 9.
We designed a vector for cassette mutagenesis (Wells et al., ~, 34, 315-323 [1985)) and expression of the hGH-gene III fusion probin with the objectives of (1 ) improving the wnkage between hGH and the gene I II
moiety to more favorably display the hGH moiety on the phage (2) limiting expression of the fusion protein to obtain essentially 'monovaleM display,' (3) allowing for restriction rxidease selection against the starting vector, (4) eliminating expression of fusion protein from the starting vector, and (5) achieving taale expression of the corresponding free hormone from a given hGH-gene III fusion mutant.
Plasmid pS0643 was constructed by oligonuGeotide-directed mutagenesis (Kunkel et al., Fp~,154, 367-382 [1987J) of pS0132, which contains pBR322 and fi origins of repbcation and expresses an hGH-gene III fusion protein (hGH residues 1-191, followed by a single Gly residue, fused to Pro-198 of gene III) under the control of the F,,~ p(ZQ6 promoter (Bass et al., Proteins 8, 309-314 [1990])(Figure 9). Mutagenesis was carried out with the oligonuGeotide 5'-GGC-AGC-TGT-GGC-TT_r,~AG-AGT-GGC-GGC-GGC-TCT-GGT-3', which introduces a ~[ site (underlined) and an amber stop colon (TAG) foNowing Phe-19t of hGH. In the resulting construct, pS0643, a portion of gene III was deleted, and two silent mutations (underlined) occurred, yielding the folbwing junction between hGH and gene III:
__ ~ _______.__________.> geae » >
2 5 1B7 188 18B 190 181 am' 249 2!!0 261 2:3~ 2S3 264 t9Q Gars G19 P>'e (;fin tsa Cdr G~ Gf;Y ~' ~9 GGC AOC TGT GGA ThC TAG ~IGT O0~ (ifs'T' C1GC TCT GiG1' This shortens the total size of tire fusion protein firom 401 residues ~
pS0132 to 350 residues in 3 0 pS0643. Experiments using monoclonal antibodies against hGH have demonstrated that the hGH portion of the new fusion protein, assemded on a phage particle, is more acoesside than was the previous, longer fusion.
For propagation of hormone-displaying phage, pS0643 and derivatives can be grown in a amber-suppressor strain of ~, such as JM101 or XL1-Blue (Bullock et al., BioTecbpj~
5, 376-379 [1987j). Shown above is substitution of Glu at the amt~er colon which occurs in ;~ suppressor strains. Suppression with other 3 5 amino acids is also possible in various available sUair~s of ~,,~ well known and publicalty available.
To express hGH (or mutants) tree of the gene III portion of the fusion, pS0643 and derivatives can simply be groHm in a non-suppressor strain such as 16C9. ~ ihis case, the amber colon (TAG) leads to termination of translation, which yields free hormone, without the need for an independent DNA construction.
To create sites for cassette mutagenesis, pS0643 was mutated with the oligonucJeotides (1 ) 5'-CGG-40 ACT-GGG-CAG-ATA-TTC-AAG-CAG-ACC-3', which destroys the unique $q(1[ site of pS0643; (2) 5'-CTC-AAG-AAC-TAC-GGG-TTA-CCC-TGA-CTG-CTT-CAG-GAA-GG-3', which inserts a unique ~j site, a single-base (rameshift, and a non-amber stop colon (TGA); and (3) 5'-CGC-ATC-GTG-CAG-TGC-AGA-TCT-GTG-GAG-GGC-3', which introduces a new ~ site, to yield the starting vector, pH0509. The addition of a frameshift along with a TGA stop colon insures that no genelll-fusion can be produced from the startirg vector.
WO 92/09690 ~ Q ~ PCT/LJS91/09133 The ~[[ - ~[[1 segment is cut out of pH0509 and replaced with a DNA cassette, mutated at the colons of interest. Other restriction sites for cassette mutagenesis at other locatans in hGH have also been introduced into the hormone-phage vector.
Colons 172,174, 176 and 178 of hGH were targeted for random mutagenesis because they all lie on or near the surface of hGH and contribute significantly to receptor-binding (Cunningham and Wells, 244, 1081-1085 (1989]); they all we within a well-defined structure, occupying 2'tums' on the same side of helix 4;
and they are each substituted by at least one amino aad among krawn evolutionary variants of hGH.
We chose to s~stitute NNS (N=A/G/C/T; S=G/C) at each of the target residues.
The choice of the NNS degenerate sequence yields 32 possible codor~s (including at least one colon for each amino aad) at 4 sites, for a total of (32)4= 1,048,576 possible nucleotide sequences, or (20)4=
160,000 possible polypeptide sequences. Only one stop colon, amber (TAG), is albwed by this choice of colons, and this colon is suppressible as Glu in ~ strains of ~.
Two degenerate oligonudeotides, with NNS at colons 172,174,176, and 178, were synthesized, phosphorylated, and annealed to construct the mutagenic cassette: 5'-GT-TAC-TCT-ACT-GCT-TTC-AGG-AAG-GAC-ATG-GAC-NNS-GTC-NNS-ACA-NNS-CTG-NNS-ATC-GTG-CAG-TGC-A-3', and 5'-GA-TCT-GCA-CTG-CAC-GAT-SNN-CAG-SNN-TGT-SNN-GAC-SNN-GTC-CAT-GTC-CTT-CCT-GAA-GCA-GTA-GA-3'.
The vector was prepared by digesting pH0509 with followed by ~[[j. The products were run on a 1% agarose gel and the large fragment exased, phenol-extracted, and ethanol precipitated. This fragment was treated with calf intestinal phosphatase (Boehringer), then phenol:chloroform extracted, ethanol preapitated, and resuspended for ligatan with the mutagenic cassette.
rr~~umn uu: nnu~ nu~~r m w~rw~ ma Following ligation, the reaction products were again digested with , then phenol:chloroform extracted, ethanol precipitated and resuspended in water. (A g~j1 recognition site (GGTNACC) is created within cassettes which contain a ~ at position 3 of colon 172 and an ~ (Thr) colon at 174. However, treatment with ~stEll at this step should not select against any of the possible mutagenic cassettes, because virtually all cassettes will be heteroduplexes, which cannot be Geaved by the enzyme.) Approximately 150 ng (45 fmols) of DNA was electroporated into XL1-Blue cells (1.8 x 109 cells in 0.045 mL) in a 0.2 an cuvette at a voltage setting of 2.49 kV with a single pulse (time constant = 4.7 msec.).
The cells were allowed to recover 1 hour at 37oC in S.O.C meda with shaking, then mixed with 25 mL
2YT medium,100 mg/mL carbenicillin, and M13-K07 (moi= 100). After 10' at 23oC, the culture was incubated overnight (15 hours) at 37oC with shaking. Plating of serial dilutions from this culture onto carbenicillin-containing media indicated that 3.9 x 107 electrotransformants were obtained.
After overnight incubation, the cells were pelleted, and double-strarxied DNA
(dsDNA), designated pH0529E (the initial library), was prepared by the alkaline lysis method. The supernatant was spun again to remove any remaining cells, and the phage, designated phage pool ~H0529E (the initial lilxary of phage), were PEG-precipitated and resuspended in 1 mL STE buffer (10 mM Tris, pH 7.6, 1 mM
EDTA, 50 mM NaCI). Phage WO 92/09690 ~ j ~ ~ ~ PCT/US91/09133 titers were measured as cobny-forming units (CFU) for the recombinant phagemid containing hGH-gap.
Approximately 4.5 x 1013 CFU were obtained from the starting library.
From the pool of electrotransformants, 58 doves were sequenced in the region of the ~-gq(j[
cassette. Of these, 17% corresponded to the starting vector,17% contained at least one frame shift, and 7°~6 contained a non-silent (non-terminating) mutation outside the four target colons. We conclude that 41°~ of the doves were defective by one of the above measures, leaving a botat functional pool of 2.0 x 107 initial transfortnants. This number stiN exceeds the possible number of DNA sequences by nearly 20-fold. Therefore, we are confident of having all possible sequences represented in the starting litxary.
We examined the sequences of non-selected phage to evaluate the degree of colon bias in the mutager~esis (Table V). The results indicated that, although some colons (and amino adds) are under- or over-represented relative to the random expectation, the library is extremely diverse, with no evidence of large-scale 'sibling' degeneracy (Table VI).
Table V.
Colon distributan (per 188 colons) of non-selected hormone phage. Cbnes were sequenced from the starting library (pFt0529E). All colons were tabulated, inducting those from Bones which contained spurious mutations andlor frameshifts. ' Note: the amber stop colon (TAG) is suppressed as Glu in XLt-Blue cells. Highlighted colons were ovedunder-represented by 50% or more.
leu 17.6 18 1.0 Ser 17.6 26 1.5 A r g 17.6 10 0.57 Pro 11.8 16 1.4 Thr 11.8 14 1.2 Ala 11.8 13 1.1 Gly 11.8 16 1.4 Val 11.8 4 0.3 1e 5.9 2 0.3 Met 5.9 1 0.2 Ty r 5.9 1 0.2 Ws 5.9 2 0.3 Trp 5.9 2 0.3 Phe 5.9 5 0.9 4 Cys 5.9 5 0.9 Gln 5.9 7 1.2 Asn 5.9 14 2.4 Lys 5.9 11 1.9 Asp 5.9 9 1.5 Glu 5.9 6 1.0 amber' 5.9 6 1.0 WO 92/09690 ~ ~ ~ ~ ~ PCT/US91/09133 Table VI.
Non-selected (pH0529E) clones with an open reading frame.
The notation, e.g. TWGS, denotes the hGH mutant 172TI174WI176G/178S. Amber (TAG) colons, translated as GIu in XL1-Blue cells are shown as E.
Ke NT KTEQ CVLQ
TWGS NNCR EASL
Pe ER FPCL SSKE
LPPS NSDF ALLL
SLDP HRPS PSHP
OQSN LSLE SYAP
GSKT NGSK ASNG
TPVT LTTE EANN
RSRA PSGG KNAK
LCGL LWFP SRGK
TGRL PAGS GLDG
AKAS GRAK NDPI
GNDD GTNG
Immobilized hGHbp ('hGHbp-beads') was prepared as described (Bass et al., Proteins 8, 309-314 [1990]), except that wild-type hGHbp (Fuh et al., ~, Biol. Chem. 265, 3111-3115 [1990]) was used. Competitive binding experiments with [1251] hGH indicated that 58 fmols of turxtional hGHbp were coupled per uL of bead Immobilized hPRLbp ('hPRlbp-beads') was prepared as above, using the 211-residue extraoelluiar domain of the prolactin receptor (Cunningham et al., ~jg~ 250,1709-1712 [1990]). Competitive binding experiments with [1251] hGH in the presence of 50 l,t~ zinc indicated that 2.1 fmols of functional hPRLbp were 3 0 coupled per N.L of bead suspension.
'Blank beads' were prepared by treating the oxirane-acrylamide beads with 0.6 M etharalamine (pH
FIELD OF THE INVENTION
This invention relates to the preparation and systematic selection of novel binding proteins having altered binding properties for a target molecule. Specifically, this irwentan relates to methods for producing foreign polypeptides mimicking the binding activity of naturally occurring binding partners. In preferred embodiments, the invention is directed to the preparation of therapeutic or diagnostic compounds that mimic proteins or nonpeptidyl mole~es such a hormones, dings and other smaN
moleales, particularly biologically active molea~les such as growth hormone.
BACKGROUND OF THE INVENTION
Binding partners are substances chat speafically bind to one another, usually through noncovalent interactions. Examples of binding partners inGude ligand-receptor, antibody-antigen, drug-target, and enzyme-substrate interactions. Binding partners are extremely useful in both therapeutic and diagnostic fields.
Binding partners have been produced in the past by a variety of methods including; harvesting them from nature (e.g., antibody-antigen, and ligand-receptor pairings) and by adventitious identification (e.g.
traditional drug development empbying random screening of candidate molecules). In some instances these two approaches have been combined. For example, variants of proteins or pdypeptides, such as polypeptide fragments, have been made that contain key functional residues that participate in binding. These polypeptide fragments, in tum, have been derivatized by methods akin to traditional drug development. M example of such derivitization would include strategies such as cyclization to confortnationally constrain a polypeptide fragment to produce a novel candidate binding partner.
The problem wish prior art methods is that naturally occurring ligands may not have proper characteristics for all therapeutic applications. Additionally, polypeptide Ggands may not even be available for some target substances. Furthermore, methods for making non~naturally occurring synthetic binding partners are often expensive and difficult, usually requiring complex synthetic methods to produce each candidate. The inability to characterize the structure of the resulting candidate so that rational drug design methods can be applied for further optimizatan of candidate molecules further hampers these methods.
In an attempt to overcome these problems, Geysen (Geysen, Immun. Todav. 6:364-369 [1985]); and (Geysen et al., ~~p~p" 23:709-715 [1986J) has proposed the use of polypeptide synthesis to provide a framework for systematic iterative binding partner identification and preparation. According to Geysen et al., Ibid, short polypeptides, such as dipeptides, are first screened for the ability to bind to a target molecule. The most active dipeptides are then selected for an additional round of testing comprising linking, to the starting dipeptide, an additional residue (or by internally modifying the components of the original starting dipeptide) and then screening this set of candidates for the desired activity. This process is reiterated until the Minding partner having the desired properties is identified.
The Geysen et at. method suffers from the disadvantage that the chemistry upon which it is based, peptide synthesis, produces molecules with ill-defined or variable secondary and tertiary structure. As rounds of iterative selection progress, random interactions accelerate among the various substituent groups of the polypeptide so that a true random population of interactive molecules having reproducible higher order structure becomes lays arid less adainabla. For example, inleractians beMleen side drains of amino acki5. which are sequentiasy widely aeparathd but which are spatialH nelgt,mrs. freely ocax.
Furihertnore, sequences that do not taa~tate confartnationally stable secondary sbuGtureS ~~ ~P~x t~et~-ddectr3in interactions wtxcn may prevent sideChaln in6eracifons of a given amino dad with tyre target motearle.
Such complex interactions are -g taalitated by the Nexittility of die polyamlde back 8t ttta pdypeptide candidates. Additionally, rrandldates may exist In numerous aontormAliOrts mafdn9 it dlmCult to identity the oarfartner shat interacts or binds to the target with greatesl alenity or speatrcity eamplicating rational drug des'sgn-A final problem with the ipsrative polypeptide method of Geyttett is that, at presenl, there are r~
practical medrods with which a great diversity of dllferent peptides can be pnvduoed. screened and analyzed. i3y 10 using the Iwenty naturatty Qoaming amino acids, the total number of all oambinatbrrs of hexapeptides that must be synthesized is 64,t>00,000. Even having prepared such a dversity at peptides, there are no methods avrailabte with which mixtrxes of sub a diversity of peptides aan ~ raP~Y Keened to selea those pepdde5'havir~g a high atkniiy for the target molecule. At present, each 'adtrerent' peptide must be recovered in amounts large enough tQ cony out protein sequenoin9~
15 To overcome marry et the problems inherent in the ~y~n aPP~ ~~i~l selection and 5aeenlng was ciwsen as an attemadve. Biological selections and saeens are Powerful foals to probe Protein function and to isolate variant proteins with desirable properties (Shortle, plpi!»Dg. Oxender and Fox, ads., A.R. Liss, Inc., NY, pp.103~108 [1988]) and 8ovne et or.. ., ~T:1306-1310 [199D)).
However, a given selection or gcreen is appliGabIB t0 only Dne or a small number of related proteins.
20 Recendy, umilh and coworkers (Smith. ~~. x:1315~1317 [1985)) arKi Parmley and Smith. ~.
7a:3a5-318 [1985] have der~nstrated that small protein tra9ments (10~50 amino adds) can be 'displayed' etfidently on the surface of iilamentous pf~ge by inserting Short gene lsagmerns Into genQ III of the td phage (~tusion phage'). The gene Ill minor coat protein (present in abo~ 3 ~Ples at one end at the virion) is important for proper pttage assembly and tar infection by attachment to the pill of E
Colt (see Ranched et at. , 25 gsy"50: a01-427 [1886]). Recently, 'fusion pttage' have been shown to be useful for displaying start mutated peptide sequences for identifying peptides Ihat may react with anUbadies (Scott et 8i., 249: 386-390, [1990] -..)and Gwirla et tit., prn~ Na_N, p,ad t A 87: 638~6382. [1990[).or a foreign protein (Llevlin et al"
~~' ,pig, 24~: 404-406 [t990)).
There are, trowever, several important limitations In using tttxh'fusian ptrage' to ider>diy altered 30 pepCides or proteins with new or enhanced birxJitlg fxapeTt~s. Frst, it has teen shown (Parmldy et al., one. 73:
305-318. [1998]) that fusion phage are useful only br displaying proteins of less Ihan tOQ arid preferably less than 50 amino aad residues, becauso large inserts presumably disrupt the krrction of Gene Ill and ii~refore phage assembly and intectivity. Second, prior art methods have been unable b select pep4des from a Gtxary having the highest binding aeiNty for a target molecule. For example, attar exhaustive panning of a random peptide litxary 35 with an antr~ endorphin monoGOrral antiltcdy, t~lrla et al., supra could not separate moderate adinity peptides (icd - 10 wM) from higher affinity peptides (Kd -d.4 ~M) fused id phage. Moreover, the Parent ~_ endorphin peptide sequence which has very trigh aftirtity (iCd ~ 7nMj, was not Padre from the epitope library.
Ladner WO 9010x802 discloses a method for selecting novel birxkrtg proteins displayed on the ouoer surface of cells arid viral particles where it is contemplated drat the heharobgorss proteins may have up to 1 B4 WO 92/09690 ~ ~ ~ PCT/U591 /09133 3 >._ amino acrd residues . The method cont~nplates isolating and amplifying the c~splayed proteins to engineer a new family of Minding proteins having desired affinity for a target moleade. More sped6calty, Ladner discloses a 'fusion phage' displaying proteins having 'initial protein finding domains' ranging from 46 residues (cramt~n) to 164 residues (T4 lysozyme) fused to the M13 gene III coat protein. Ladner beaches the use of proteins'no larger than necessary t~erause it is easier to arrange restriction sites in smaNer amino add sequences and prefers the 58 amino add residue bovine pancreatic trypsin inhibitor (BPTI). Small tusan proteins, such as BPTI, are preferred when the target is a protein or macromolecule, while larger fusion proteins, such as T4 lysozyme, are preferred for small target molecules such as sterads because such large proteins have clefts and grooves into which small molecules can fit. The preferred protein, BPTI, is proposed to be fused b gene III at the site disclosed by Smith et al. or de la Cruz et al., J. Biol. Chgm" 263: 4318,4322 [1988), or to one of the terminii, along with a second synthetic copy of gene III so that'some' unaltered gene III protein will be present. Ladner does not address the problem of successfully panning high affinity peptides from the random peptide library which plagues the biological selection and screening methods of the prig art.
Human growth hormone (hGH) partidpates in much of the regulation of normal human growth and development. This 22,000 dalton ptuitary hormone exhibits a multitude of biological effects including linear growth (somatogenesis), lactation, activation of macrophages, insulin-like and diabetogenic effects among others (Chawla, R, K. (1983) 911p ev. Med. fig, 519; Edwards, C. K et al. (1988) ,~,p~,~Q, 769; Thomer, M. 0., et al.
(1988) J. Clip. Invest gl, 745). Growth hormone defiaency in children leads to dwarfism which has been successfully treated for more than a decade by exogenous administratan of hGH.
hGH is a member of a family of 2 0 homologous hormones that include placenhal lactogens, prolactins, and other genetic and spades variants or growth hormone (Nicoll, C. S., efal., (1986) ~ocrine Reviews 2,169). hGH is unusual among these in that it exhibits broad species spedfidty and binds to either the dor~ed somatogenic (Leung, D. W., et aL, [1987] ~g ~,,3,Q, 537) or prolactin receptor (Boutin, J. M.,et al., [1988] fig; ,~, 69). The doped gene for hGH has been expressed in a secreted forth in ~j (Ctrarg, C. N., et al., [i987] ~;~,189) and its DNA and amino add sequence has been reported (Goeddel, etal., p979] ~,~, 544; Gray, etal., [1985] ~,3$, 247).
The three-dimensional stnxture of hGH is not available. However, the three-dimensional folding pattern for porcine growth hormone (pGH) has been reported at moderate resolution and reffr~ement (Abdel-Meguid, S. S., et al., [1987j Proc-Natl.Natl.
e~~d. Sci. USA $4, 6434). Human growth hormone's receptor and antibody epitopes have been identified by homolog-scanning mutagenesis (Cunningham etal., Saenoe Zq$;1330,1989). The structure of novel amino terminal methionyl bovine growth hormone contair>ing a spliced-in sequerxe of human growl hormone including histidine 18 and histidine 21 has been shown (U.S. Patent 4,880,910) Human growth hormone (hGH) cages a variety of physiological and metabolic effects in various ar>imal models including linear bone growth, lactation, activation of macrophages, insulin-like and diabetogenic effects and others (R. K. Chawla etal., Anna. Rev. Mad. 34, 519 (1983); 0. G. P. Isaksson etat., Mrw. Rev. Phys'rot. 47, 483 (1985); C. K. Edwards etal., Science 239, 769 (1988); M. 0. Thomer and M. L.
Vance, J. Clip. Invest. 82, 745 (1988); J. P. Hughes and H. G. Friesen, Mn. Rev. Physiol. 47, 469 (1985)).
These biological effects dertve from the interaction between hGH and spedfic cellular receptors..
Accordingly, it is an object of this invention to provide a rapid and effective method for the systematic preparation of candidate binding substances.
It is another object of this invention to prepare candidate Minding substances displayed on surface of a phagemid particle that are conformationally stable.
It is another object of this invention to prepare candidate tending substances comprising fusion proteins of a phage coat protein and a heterologous polypeptide where the polypeptide is greater than 100 amino acids in length and may be more than one subur>;t and is displayed on a phagemid particle where the polypeptide is encoded by the phagemid genome.
It is a further object of this invention to provide a method for tt~e preparation and selection of binding substances that is suffiaently versatile to present, or display, all peptidyl moieties that could potentially particlpate in a nonoovalent binding interaction, and to present these moieties in a fashion that is sterically confined.
Still another object of the invention is the production of growth hormone variants that exhibit stronger affinity for growth hormone receptor and binding protein.
It is yet another ot~ject of this invention to produce expressan vector phagemids that contain a suppressible termination oodon tunc6onally bcated between the heterok~gous polypeptide and the phage coat protein such that detectable fusion protein is produced in a host suppressor cell and only the heterologous polypeptide is produced in a non-suppresser host cell.
Fnally, it is an object of this invention to produce a phagemid particle that rarely displays more than one copy of candidate binding proteins on the outer surface of the phagemid particle so that efficient selection of high affinity tHnding proteins can be achieved.
These and other objects of this irnention wit be apparent from consideration of the invention as a whole.
These objectives have been achieved by providing a method for seleding novel binding polypeptides comprising: (a) constructing a replicable expression vector comprising a first gene encoding a polypeptide, a second gene encoding at least a portion of a natural or wild-type phage coat protein wherein the first and second 2 5 genes are heterologous, and a transcription regulatory element operady linked to the first and second genes, thereby forming a gene fusion erxxxfing a fusion protein; (b) mutating the vector at one or more selected positions within the first gene thereby forming a family of related plasmids; (c) transforming suitable host peas with the plasmids; (d) infecting the transformed host cells with a helper phage having a gene encoding the phage coat protein; (e) culturing the transformed infected host cells under conditions suitable for forming recombinant phagemid particles containing at least a portion of the plasmid and capable of transforming the host, the conditions adjusted so that no more than a minor amount of ph~emid particles display more than one copy of the fusion protein on the surface of the particle; (f) contacting the phagemid particles with a target molecule so that at least a portion of the phagemid particles find to the target molecule; and (g) separating the phagemid particles that bind from those that do not. Preferably, the method further comprises transforming suitable host cells with recombinant phagemid particles that bind to the target molecule and repeating steps (d) through (g) one or more times.
Additionally, the method for selecting novel binding proteins where the proteins are composed of more than one subunit is ad~ieved by selecting novel binding peptides comprising constructing a replicable expression vector comprising a transcription regulatory element operably linked to DNA
erxoding a protein of interest WO 92/09690 ~ ~~ ~ ~ ~ ~ ~ PC1'/US91/09133 _ containing one or more subunits, wherein the DNA encoding at least one of the subunits is fused to the DNA
encoding at least a portion of a phage coat protein;mutating the DNA encoding the protein of interest at one or more selected positions thereby fomring a family of related vectors;
transforming suitable host cells with the vectors; iMecting the transformed host cells with a helper phage having a gene encoding the phage coat protein;
5 culturing the transformed infected host cells under conditions suitable for forming recombinant phagemid particles containing at least a portion of the plasmid and capable of transforming the host, the conditions adjusted so that no more than a minor amours of phagemid particles display more than one copy of the fusion protein on the surface of the particle; contacting the phagemid particles with a target molecule so that at least a portion of the phagemid particles bind to the target molecule; and separating the phagemid particles that hind from those that do not.
Preferably in the method of Ihis invention the plasmid is under tght control of the transcription regulatory element, and the culturing conditions are adjusted so that the amount or number of phagemid particles displaying more than one copy of the fusion protein on the surface of the particle is less than about 1 %. Also preferably, amount of phagemid particles displaying more than one copy of the fusion protein is less than f0% the amount of phagemid particles displaying a single copy of the tusan protein.
Most preferably the amount is less than 20°x.
Typically, in the method of this invention, the expression vector will further contain a secretory signal sequences fused to the DNA enaxling each subunit of the polypeptide, and the transcription regulatory element will be a promoter system. Preferred promoter systems are selected from; Lac Z, a,pL, TAC, T 7 polymerise, tryptophan, and alkaline phosphatase promoters and combinatans thereof.
Also typically, the first gene will encode a mammalian protein, preferably the protein will be selected from; human growth hormone(hGHj, N-metfionyl human growth hormone, bovine growth homrone, parathyroid hormone, thyro>one, insulin A-drain, insulin B~chain, proinsuWn, rolaxin A~hain, relaxin B-chain, prorelaxin, glycoprotein hormones such as follicle stimulating hormone(FSH), thyroid stimulating hormone(TSH), and leutinizing hormone(LH), glycoprotein hormone receptors, caldtonin, glucagon, factor VIII, an antibody, lung surtactant, urokinase, streptokinase, human tissue-type plasminogen activator (t-PA), bombesin, factor IX, thrombin, hemopoietic growth factor, tumor r~sis factor-alpha and -beta, enkephalinase, human serum albumin, mullerian-inhibiting substance, mouse gonadotropin-associated peptide, a microbial protein, such as betalactamase, tissue factor protein, inhibin, activin, vascular endothelial growth factor, receptors for hormones or growth factors; integrin, thrombopoietin, protein A or D, rheumatoid factors, nerve growth factors such as NGF-)3, platelet~rowth factor, transforming growth factors (TGFj such as TGF,alpha and TGF-beta, insuiin-like growth factor-I and -II, insulin-like growth factor binding proteins , CD-4, DNase, latency assodated peptide, erythropoietin, osteoinductive factors, interferons such as interferon-alpha, -beta, and -gamma, colony stimulating factors (CSFs) such as M-CSF, GM-CSF, and G-CSF, interleukins (ILs) such as IL-1, IL-2, IL-3, IL-4, superoxide dismutase; decay accelerating factor, viral antigen, HIV
envelope proteins such as GP120, GPt40, atrial natriuretic peptides A, B or C, immunoglobulins, and fragments of any of the above-listed proteins.
Preferably the first gene will encode a polypeptide of one or more subunits containing more than about 100 amino add residues and will be folded to form a plurality of rigid secondary structures displaying a plurality of amino acids capable of interacting with the target. Preferably the first gene will be mutated at codons corresponding to only the amino acids capable of interacting with the target so that the integrity of the rigid secondary strur.~ures will be preserved.
Normally, the method of this invention will empby a helper phage selected from; M13K07, M13R408, M13-VCS, and Phi X 174. The preferred helper phage is M13K07, and the preferred coat protein is the M13 Phage gene III coat protein. The preferred host is E. cbli, and protease deffdent strains of E. coli. Novel hGH
variants selected by the method of the present irnentan have been detected.
Phagemid expression vectors were constnxted that contain a suppresside termination colon functionally located between the nucleic acids encoding the polypeptide and the phage coat protein.
BRIEF DESCRIPTION OF THE FIGURES
FIGURE 1. Strategy for displaying large proteins on the surface of filamenbous phage and enriching for altered receptor binding properties. A plasmid, phGH-Ml3glll was consrruucted that fuses the entire coding sequence of hGH to the carboxyl terminal domain of M13 gene III. Transcription of the fusion protein is under control of the lac promoberloperator sequence, and secretion is directed by the stll signal sequence. Phagemid partices are produced by infection with the 'helper phage, M13K07, and particles displaying hGH can be enriched by twining to an affinity ma~ix contakring the hGH receptor. The wild-type gene III (derived from the M13K07 phage) is diagramed by 4-5 copies of the multiple arrows on the tip of the phage, and the fusion protein (derived from the phagemid, phGH-Ml3glll) is indicated schematicatiy by the folding diagram of hGH repladng the arrow head.
FIGURE 2 knmunot~lot of whole phage particles shows that hGH comigrates with phage. Phagemid 2 0 particles purified in a cesium chloride gradient were loaded into duplicate wells and electrophoresed through a 1 agarose gel in 375 mM Tris, 40 mM glycne pH 9.6 buffer. The gel was soaked in transfer buffer (25 mM Tris, pH
8.3, 200 mM glyane, 20% methanol) containing 2% SDS and 296 ~-mercaptoethanol for 2 hours, then rinsed in transfer buffer for 6 hours. The proteins in the gel were then electrobbtted onto immot>ilon membranes (Millipore). The membrane containing one set of samples was stained with Coomassie blue to show the position of the phage proteins (A). The duplicate membrane was immures-stained for hGH by reacting the membrane with polydonal rabbit anti-hGH antibodies folbwed by reaction with horseradish peroxidase conjugated goat anti-rabbit IgG antibodies (B). Lane 1 contains Ifrre M13K07 parent phage and is viside only in the Coomassie blue stained membrane, since it lacks hGH. Lanes 2 and 3 contain separate preparations of the hormone phagemid partiGes which is visible both by Coomassie and hGH immuno-stairang. The difference in mgration distance between the parent M13K07 phage and hormone phagemid particles reflects the different size genomes that are packaged within (8.7 kb vs. 5.1 kb, respectively).
FIGURE 3. Summary diagram of steps in the selection process for an hGH-phage library randomized at colons 172,174,176, and 178. The template molecules, pH0415, containing a unique Kpnl restriction site and the hGH(Rt78G,1179T) gene was mutagenized as described in the text and electrotransfonned into E. cofi strain WJM101 to obtain the initial phagemid library, Library 1. An aliquot (approximately 2%) from Ubrary 1 was used directly in an initial selection round as described in the text to yield Litxary 1 G. Meanwhile, double-stranded DNA
(dsDNA) was prepared from Library I, digested with restriction enzyme Kpnl to eliminate template badkground, and electrotransformed into WJM101 to yield Library 2. Subsequent rounds of selection (or Kpnl digestion, shaded boxes) followed by phagemid propagation were carried out as indicated by the arrows, according to the WO 92/09690 ~ '~ ,-~~ ~ ~ ~ ~ PCT/US91 /09133 ..
procedure described in the text. Four independent doves from litxary 4G4 and tour independent Bones from library 5G6 were sequenced by dideoxy sequendng. All of these Bones had the identical DNA sequence, corresponding 1o the hGH mutant (Glu 174 Ser, Phe 176 Tyr).
FIGURE 4. Structural model of hGH derived from a 2.8 la folding diagram of porcine growth hormone determined aystallographically. t-ovation of residues in hGH that strongly modulate its binding to the hGH-binding protein are within the shaded drde. Alanine substitutions that cause a greater than tenfold reduction(~), a four- to tenfold reduction (~), or increase (O), or a two- to fourfold reduction (~), in tending affinity are indicated. Helical wheel projections in the regions of a-helix reveal their amptupathic quality.
Blackened, shaded, or noruhaded residues are charged, polar, or nonpolar, respedavely. In helix-4 the most important residues for mutation are on the hydrophilic face.
FIGURE 5. Amino acrd substitutions at positions 172,174,176 and 178 of hGH
(The notation, e.g.
KSYR, denotes hGH mutant 172KI174SI176Y/178R.) found after sequencng a number of doves from rounds 1 and 3 of tt~e selection process for the pathways indicated (hGH elution;
Glycine elution; or Glydne elution after pre-adsorption). Non-functional sequences (i.e. vector badkground, or other prematurely terminated andlor frame-shifted mutarns) are shown as'NF'. Fkxrctional sequences which contained a non-silent, spurious mutation (i.e. outside the set of target residues) are marked with a '+'. Protein sequences which appeared more than once among all the sequerxed doves, but with different DNA sequences, are marked with a '~". Protein sequerxes wtych appeared more than once among the sequenced Bones and with the same DNA
sequence are marked with a "'. Note that after three rounds of selection, 2 different contaminating sequences were found; these Bones did not correspond to cassette mutants, but to previously constructed hormone phage. The pS0643 contaminant corresponds to wild-type hGH-phage (hGH 'KEFR'). The pH0457 contaminant, which dominates the third-round glycine-selected pool of phage, corresponds to a previously identified mutant of hGH, 'KSYR.' The amplification of these contaminants emphasizes the agility of the hormone-phage selection process to select for rarely oocurting mutants. The convergence of sequences is also striking in all three pathways: R or K occurs most often at positions 172 and 178; Y or F occurs most often at position 176; and S, T, A, and other residues occur at position 174.
FIGURE 6. Sequences from phage selected on hPRLbp-beads in the presence of zinc. The notation is as described in Figure. 5. Here, the convergerxe of sequerxes is not predictable, but there appears to be a bias towards hydrophotMC sequences under the most sfringent (Glydne) seledion conditions; L ,W and P residues are 3 0 frequently found in this pool.
FIGURE 7. Se~errces from phage selected on hPRLbp-beads in the absence of zinc. The notation is as described in Figure 5. In contrast to the sequences of Fgure. 6, these sequences appear more hydrophilic. After 4 rounds of selection using hGH elution, two clones (ANHQ, and TLDTI171V) dominate the pool.
FIGURE 8. Sequences from phage selected on blank beads. The notation is as described in Fg. 5. After three rounds of selection with glycine elution, no siblings were observed and a badkground level of non-functional sequences remained.
FIGURE 9. Construction of phagemid fl on from pH0415. This vector for cassette mutagenesis and expression of the hGH-gene III fusion protein was constnxted as follows.
Plasmid pS0643 was constructed by oligonudeotide-directed mutagenesis of pS0132, which contains pBR322 and f1 origins of replication and WO 92/09690 PC1'/US91/09133 expresses an hGH-gene III fusion protein (hGH residues 1-191, folbwed by a single Gly residue, fused to Pro-198 of gene III) under the control of the ~ )~ promoter. Mutagenesis was carried out with the oligonuGeotide 5'-GGC-AGC-TGT-GGC-TTC-TAG-AGT-GGC-GGC-GGC-TCT-GGT-3', which introduced a ~
site (underlined) and an amber stop colon (TAG) following Phe-191 of hGH.
FIGURE 10. A. Diagram of plasmid pDH188 insert containing the DNA encoding the Ight chain and heavy chain (variable and constant domain 1 ) of the Fab humanized antibody directed to the HER-2 receptor. V~
and VH are the variable regions for the Ight and heavy chains, respectively.
Ck is the constant region of the human kappa light chain. CH1G1 is the first constant region of the human gamma 1 chain. Both coding regions start with the bacterial st II signal sequence. B. A schematic diagram of the entire plasma pDH188 containing the insert described in 5A. After transformation of the plasmid into E. cbli SR101 cells and the addition of helper phage, the plasmid is packaged into phage particles. Some of these particles display the Fab-p III fusion (where p III is the protein enk~ded by the M13 gene III DNA). The segments in the plasmid figure correspond to the insert shown in 5A.
FIGURE 11 A through C are cdlectJvely referred to here as Figure 11. The nucleotide (Seq. ID No. 25) sequerxe of the DNA encoding the 4D5 Fab molecule expressed on the phagemid surface. The amino acid sequerx;e of the light chain is also shown (Seq. ID No. 26), as is the amino acct sequence of the heavy chain p III fusion (Seq. ID
No. 27).
FIGURE 12 Enrichment of wild-type 4D5 F~ phagemid from variant Fab phagemid.
Mixtures of wild-type phagemid and variant 4D5 Fab phagemid in a ratio of 1:1,000 were selected on plates coated with the extra-cellular domain protein of the HER-2 receptor. After each round of selection, a portion of the eluted phagemid were infected into E. Qoli and plasmid DNA was prepared. This plasmid DNA was then digested with Eco RV and Pst I, separated on a 5% polyakxylamide gel, and stained with ethidium bromide. The bands were visualized under UV light. The bands due to the wild-type and variant plasmids are marked with arrows. The first round of selection was eluted only under acid conditions; subsequent rounds were eluted with either an acct elution (left side of Figure) or with a humanized 4D5 antibody wash step prior to acid elution (right side of Figure) using methods desalted in Example VIII. Three variant 4D5 Fab molecules were made:
H9t A (amino aad histidine at position 91 on the V~ chain mutated to alanine; indicated as 'A' lanes in Figure), Y49A (amino acid tyrosine at position 49 on the V~ chain mutated to alanine; indicated as'B' lanes in the Figure), and Y92A (amino acid tyrosine at position 92 on the V~ chain mutated to alanine; indicated as'C' lanes in the Fgure). Amino acct position numbering is according to Kabat et al.,(Sequences of proteins of immunological interest, 4th ed., U.S. Dept of Health and Human Services, Public Health Service, Nat'I. Institute of Health, Bethesda, MD (1987]).
FIGURE 13. The Scatchard analysis of the RIA affinity determination described in Experimental Protocols is shovm here. The amount of labeled ECD antigen that is bound is shown on the x-axis while the amount that is bound divided by the amount that is free is shown on the y-axis. The slope of the line indicates the Ka; the 3 5 calculated Kd is l ll(a.
WO 92/09690 2 ~ ~ ~ ~ J 3 PCT/US91/09i33 DETAILED DESCRIPTION OF THE INVENTION
The foNowing discussion will be best iurderstood by referring to Figure t. in its simplest torm, the method of the instant invention comprises a method for selecting novel binding polypeptides, such as protein Igands, having a desired, usuaay high, aiffnity for a target molecule from a library of stnxturally related bindirg polypeptides. The lilxary of structurally related polypeptides, fused Do a phage coat protein, is produced by mutagenesis and, preferably, a single copy of each related polypeptide is displayed on the surface of a phagemid particle containing DNA encoding that polypepade. These phagemid particles are then contacted with a target molecule and those particles having the highest affinity for the target are separated from those of lower affinity.
The high atfir>ity binders are then amplified by infection of a bacterial host and the competitive binding step is repeated. This process is reiterated until polypeptides of the desired affinity are obtained.
The novel binding polypeptides or ligands produced by the method of this invention are useful per se as diagnostics or therapeutics ( eg. agonists or antagonists) used in treatment of biological organisms. Structural analysis of the selected polypeptides may also be used to fadlitate rational drug design.
By 'binding polypeptide' as used herein is meant any polypeptide that binds with a selectable affinity to a target molecule. Preferably the polypeptide will be a protein that most preferably contains more than about 100 amino add residues. Typically the polypeptide will be a t~onnone or an antibody or a fragment thereof.
By 'high affinity' as used herein is meant an affinity constant (Kd ) of <t0-5 M and preferably <10'~M
under physalogical conditions.
By 'target molea~le' as used herein is meant any molecule, rat necessarily a pr~ein, for which it is desirable to produce a ligand. Preferably, however, the target will be a protein and most preferably the target will be a receptor, such as a hormone receptor.
By 'humanized antibody' as used herein is meant an antitxxly in wtich the oomplementarity~ietermining regions (CDRs) of a mouse or other ron-human antibody are graffed onto a human antibody framework. By human antibody framework is meant the entire human antibody excluding the CDRs.
L
The first step in the method of this invention is to choose a polypeptide having rigid secondary structure exposed to the surface of the polypeptide for display on the surface of a phage.
By'polypeptide' as used herein is meant any molecule whose expressan can be directed by a specific DNA sequence. The polypeptides of ttws invention may comprise more than one subunit, where each subunit is 3 0 encoded by a separate DNA sequerxe.
By 'rigid secondary structure' as used herein is meant any polypeptide segment exhibiting a regular repeated structure such as is found in; a-helices, 3fp helices, n-heMces, parallel and antiparallel ~-sheets, and reverse toms. Certain 'non~rdered' structures that lack recognizable geometric order are also included in the definition of rigid secondary structure provided they form a domain or'patch' of amino acid residues capable of interaction with a target and that the overall shape of the stnxture is not destroyed by replacement of an amino acid within the structure . h is believed that some non-ordered structures are comt~inations of reverse turns. The geometry of these rigid secondary structures is well defined by ~ and ~r torsional angles about the a-carbons of the peptide 'backbone'.
WO 92/09690 2 ~ ~ ~ ~ ~ J PCT/US91/09133 The requirement that the secondary stmct~xe be exposed to the surface of the polypeptide is to provide a domain or'patch' of amino aad residues that can be exposed to and bind with a target molecule. It is primarily these amino aad residues that are replaced by mutagenesis that form the 'library' of structurally related (mutant) Minding polypeptides that are displayed on the surface of the phage and from which novel 5 polypeptide ligands are selected. Mutagenesis or replacement of amino acid residues directed toward the interior of the polypeptide is generally avoided so that the overall stinxture of the rigid secondary structure is preserved.
Some replacement of amino acids on the interior region of the rigid secondary stnxriues, especlally with hydrophobic amino aad residues, may be tolerated since these conservative substitutions are unlikely to distort the overall structure of the polypeptide.
10 Repeated cycles of'polypeptide' selection are used to select for higher and higher affinity Minding by the phagemid selection of multiple amino aad changes which are selected by multiple selection cyGes. Following a first round of phagemid selection, involving a first region or selection of amino aclds in the ligand polypeptide, additional rounds of phagemid selection in other regions or amino aads of the ligand potypeptide are conducted.
The cycles of phagemid selection are repeated until the desired affinity properties of the ligand polypeptide are achieved. To illustrate this process, Example VIII phagemid selection of hGH
was conducted in cycles. In the first cycle hGH amino aads 172,174,176 and 178 were mutated and phagemid selected.
In a second cycle hGH amino aads 167,171,175 and 179 were phagemid selected. In a third cycle hGH amino acids 10,14,18 and 21 were phagemid selected. Optimum amino aad changes from a previous cycle may be irxorporated into the polypeptide before the next cycle of selection. For example, hGH amino aclds substitutan 174 (serine) and 176 (tyrosine) were irxorporated into the hGH before the phagemid selection of hGH amino aads 167,171,175 and 179.
From the forgoing it will be appreciated that the amino aad residues that form the binding domain of the polypeptide will not be sequentially linked and may reside on different suburuts of the polypeptide. That is, the Minding domain tracks with the particular secorxiary stnxture at the binding site and not the primary stmcture. Thus, generally, mutations will be introduced into colons erxoding amino acids within a particular secondary structure at sites directed away from the interior of the polypeptide so that they will have the potential to interact with the target. By way of illustration, Figure 2 shows the location of residues in hGH that are known to strongly modulate its t~ir~ding to the hGH-binding protein (Cunningham etaL, 247:1461-1465 (1990]). Thus representative sites suitable for mutagerresis would include residues 172, 174, 176, and 178 on helix-4, as well as residue 64 located in a 'non-ordered' secondary structure.
There is no requirement that the polypeptide chosen as a ligand to a target normally hind to that target.
Thus, for example, a glycoprotein hormone such as TSH can be chosen as a ligand for the FSH receptor and a library of mutant TSH molecules are employed in the method of this invention to produce novel drug candidates.
This invention thus contemplates any polypeptide that hinds to a target molecule, and inGudes antibodies. Preferred polypeptides are those that have pharmaceutical utility.
More preferred polypeptides 3 5 include; a growth hormone, including human growth hormone, des-N-methionyl human growth hormone, and bovine growth hormone; parathyroid hormone; thyroid stimulating hormone; thyroxine;
insulin A~chain; insulin B~hain;
proinsulin; follicle stimulating hormone; calcltorun; leutinizing hormone;
glucagon; factor VIII; an antibody; lung surfactant; a plasminogen activator, such as urokinase or human tissue-type plasminogen activator (t-PA);
t~ombesin; factor IX, thromt~in; hemopoietic growth factor; tumor necrosis factor-alpha and -beta; enkephalinase; a WO 92/09690 ~ ~ ~'~ ~ ~ ~ PCT/US91/09133 saran albumin such as txunan seem albumin; mullerian-intibiting substance;
rela~dn A~chain; relaxin B~chain;
prorelaxin; mouse gorradotropn-assoaatsd peptide; a microbial protein, such as beidta~tamase; tissue factor protein; inhibin; activin; vascular endothelial growth factor; receptors for hormones or growth factors; integrin;
thrombopoietin; protein A or D; rheumatoid factors; nerve growth factor such as NGF-J3; platelet~erived growth factor; 6broblast growth factor such as aFGF and bFGF; epidermal growth factor; transforming growth factor (TGF) such as TGF-alpha and TGF-beta; insulin-like growth factor-I and -II;
insulin-like growth factor binding proteins; CD-4; DNase; latency assodated peptide; eryttxopoietin;
osteoinductive factors; an interferon such as interteron-alpha, -beta, and -gamma; colony stimulating factors (CSFs), e.g., M-CSF, GM-CSF, and G-CSF;
interfeukins (ILs), e.g., IL-1, IL-2, IL-3, IL-4, etc.; superoxide dismutase;
decay accelerating factor; atrial natriuretic peptides A, B or C; viral antigen such as, for example, a portion of the HIV ernebpe; immunoglobulins;
and fragments of any of the above-listed polypeptides. In addition, one or more predetermined amino add residues on the polypeptide may be substituted, inserted, or deleted, for example, to produce products with improved tMOlogical properties. Further, fragments of these polypeptides, espedally biologically active fragments, are inducted. Yet more preferted polypeptides of this invention are human growth hormone , and atrial naturetic peptides A, B, and C, endotoxin, subtilisin, trypsin and Other serine proteases.
StiA more preferred are polypeptide hormones that can be defined as any amino add sequence produced in a first cell that binds spedfically to a receptor on the same cell type (autocrine hormones) or a second cell type (non-autocrine) and causes a physiobgical response characteristic of the receptor-bearing cell. Among such polypeptide hormones are cytokines, lymphokines, neurotrophic homnones and aderx>hypophyseal pdypeptide hormones such as growth hormone, prdactin, placer>tal lactogen, luteinizing hormone, follicle-stimulating hormone, thyrotropn, chorbnic gonadotropin, corticotropn, a or ~-melanocyte-stimulating hormone, J3-lipotropin, Y-lipotropin and the endorphins; hypothalmic release-inhibiting honnones such as corticotropin-release factor, growth hormone release-inhibiting hormone, growth hormone-release factor; and other polypeptide hormones such as atrial natriuretiC peptides A, B or C.
IL
The gene encoding the desired polypeptide (i.e., a polypeptide with a rigid secondary structure) can be obtained by methods known in the art (see generally, Samtxook et al. , J
olecular BiojQgw: A Labora~p~"~ap~[, Cold Spring Harbor Press, Cold Spring Harbor, New Yak [19139]). If the sequence of the gene is known, the DNA encoding the gene may be d~emically synthesized (Merrfieki, J. Am. Chem.
Soc.., 85 X149 [1963]). If the 3 0 sequence of the gene is not known, cr if the gene has rot previously been isolated, it may be cloned from a d7NA
litxary (made from RNA obtained from a suitable tissue in which the desired gene is expressed) or from a suitable genomic DNA library. The gene is then isolated using an appropriate probe. For cDNA libraries, suitable probes include monodortal or polydonal antibodies (provided that the cDNA library is an expression library), oiigonudeotides, and complementary or homologous cDNAs or fragments ri~ereof.
The probes that may be used to isolate the gene of interest from genomic DNA litxaries include cDNAs or fragments thereof that encode the same or a similar gene, homobgous genomic DNAs a DNA fragments, and oligorxxleotides. Screening the cDNA or genomic library with the selected probe is conducted using standard procedures as described in d~apters 10-12 of Samtxook et al., supra.
An alternative means to isolating the gene encoding the protein of interest is to use polymerase chain reaction methodobgy (PCR) as described in section 14 of Samlxook et al., sera.
This method requires the use of oligonudeotides that will hybridize to the gene of interest; thus, at least some of the DNA sequence for this gene must be known in order to generate the oGgonudeotides.
After the gene has been isolated, it may be inserted into a suitable vector (preferably a plasmid) for amplification, as described generally in Sambrook et al., supra.
While several types of vectors are available and may be used to practice this invention, plasmid vectors are the preferred vectors for use herein, as they may be constructed with relative ease, and can be readily amplfied. Plasmid vectors generally contain a variety of components including promoters, signal sequences, phenotypic selectan genes, origin of replication sites, and other necessary components as are known to those of ordinary skill in the art.
Promoters most commonly used in prokaryotic vectors include the )~ Z promoter system, the alkaline phosphatase p~ A promoter, the bacteriophage A,PL promoter (a temperature sensitive promoter), the ~
promoter (a hylxid ~-~ promoter that is regulated by the ~ repressor), the Iryptophan promoter, and the bacteriophage T7 promoter. For general descriptions of promoters, see section 17 of Sambrook et al. supra .
While these are the most commonly used promoters, other suitable microbial promoters may be used as well.
Preferred promoters for practicing this inventan are those that can be tightly regulated such that expression of the fusan gene can be controlled. It is believed that the problem that went unrecognized in the prior art was that display of multiple copies of the fusion protein on the surface of the phagemid particle lead to multipoint attachment of the phagemid with the target. It is believed this effect, referred to as the 'chelate effect', results in selection of false 'high affinity' polypeptides when m~dtiple copies of the fusion protein are displayed on the phagemid particle in dose proximity to one another so that the target was'chelated'. When multipoint attachment occurs, the effective or apparent Kd may be as high as the product of the individual Kds for each copy of the displayed fusan protein. This effect may be the reason Cwirla and coworkers supra were unable to separate moderate affinity peptides from higher affinity peptides.
It has been discovered that by tightly regulating expressan of the fusion protein so that ra more than a minor amount, i.e. fewer than about 1 °~, of the phagemid particles contain multiple copies of the fusion protein the 'chelate effect' is overcome allowing proper selection of high affinity polypeptides. Thus, depending on the 3 0 promoter, culturing conditions of the fast are adjusted to maximize the number of phagemid particles containing a single copy of the fusion protein and mirimize the number of phagemid particles containing multiple copies of the tusion protein.
Preferred promoters used to practice this invention are the l~ Z promoter and the ~ A promoter.
The !~ Z promoter is regulated by the lac repressor protein j~ f, and thus transcription of the fusion gene can be controlled by manipulation of the level of the lac repressor protein. By way of illustration, the phagemid containing the )~ Z promotor is grown in a ceu strain that contains a copy of the )~ f repressor gene, a repressor for the l~ Z promotor. Exemplary cell strains containing the )~ f gene include JM 101 and XL1-blue. In the alternative, the host cell can be cotransfected with a plasmid containing Moth the repressor !~ f and the I3~ Z promotor.
Occasionally both of the above techniques are used simultaneously, that is, phagmide particles containing the l~ Z
WO 92/09690 ~ ~ ,~ ~ ~ PfT/fJS91/09133 promoter are grown in oeti strains containing the ~ i gene and the ceu strains are catransfected with a plasmid containing both tt~e ~ Z and )~ i genes. Normally when one wishes to express a gene, to the transfected host above one would ~d an inducer such as isopropylthiogalacboside (IPTG). In the present invention however, this step is omitted to (a) minimize the expression of the gene III fusion protein thereby minimizing the copy number (i.e. the number of gene III iusans per phagemid number) and to (b) prevent poor or improper packaging of the phagemid caused by induoers such as IPTG even at bw corxentrations. Typcally, when no inducer is ceded, the number of fusion proteins per phagemid partide is about 0.1 (number of bulk fusion proteinslnumber of phagemid partides). The most preferred promoter used to practice this invention is p~
A. This promoter is believed to be regulated by the level of inorganic phosphate in the cell where the phosphate acts to down-regulate the activity of the promoter. Thus, by depleting cells of phosphate, the activity of the promoter can be increased. The desired result is achieved by grovhng cells in a phosphate enriched medium such as 2n or LB thereby controlling the expression of the gene III fusion.
One other useful component of vectors used to practice tNs invention is a signal sequence. This sequence is typically located immediately 5' to the gene encoding the fusion protein, and will thus be transcribed at the amino terminus of the fusan protein. However, in certain cases, the signal sequence has been demonstrated to be located at positions other 5' to the gene encoding the protein to be secreted. This sequence targets the protein to which it is attad~ed across the imer membrane of the bacterial cell. The DNA
encoding the signal sequer~e may be obtained as a restriction erxionucease fragment from any gene encoding a protein that has a signal sequence.
Suitable prokaryotic sisal sequences may be obtained from genes encoding, for example, Lama or OmpF (along et al, t~, 68:193 [1983j), MaIE, PhoA and other genes. A preferred prokaryotic signal sequence for practidng this invention is the E. cbli heat-stale enterotoxin II (STII) signal sequence as described by Chang ef a!. , ~g,pg, 55: 189 [ 1987j.
Another useful component of the vectors used to practice this invention is phenotypic selection genes.
Typical phenotypic selection genes are chose encoding proteins chat confer antibiotic resistance upon the host cell.
By way of illustration, the ampicillin resistance gene (~), and the tetracydine resistance gene (t~ are readily employed for this purpose.
Construction of suitable vectors comprising the aforementioned components as weU as the gene encoding the desired polypeptide (gene 1 ) are prepared using standard recombinant DNA
procedures as described in Samtxook et al. supra. Isolated DNA fragments to be combined m form the vector are cleaved, tailored, and 3 0 ligated together in a spedfic order and orientation to generate the desired vector.
The ONA is deaved using the appropria~ restriction enzyme or enzymes in a suitable buffer. In general, about 0.2-1 ~g of plasmid or DNA fragments is used with about 1-2 units of the appropriate restriction enzyme in about 20 p.1 of buffer solution. Appropriate buffers, DNA concentrations, and incubation times and temperatures are sped5ed by the manufacturers of the restriction enzymes.
Generally, incubation times of about one or iwo hours at 3TC are adequate, although several enzymes require higher temperatures. After incubation, the enzymes and other contaminants are removed by extraction of the digestion solution with a mixture of phenol and chloroform, and the DNA is recovered from the aqueous fraction by precipitation with ethanol.
To ligate the DNA fragments together to form a functional vector, the ends of the DNA fragments must be compatible with each other. In some cases, the ends will be directly compatible after endonudease WO 92/09690 ~ ~ ~ ~ ~ j ~~ PCT/US91/09133 digestion. However, it may be necessary to first convert the sticky ends commoNy produced by endonudease digestion to blunt ends to make them compatible for Ggation. To blunt the ends, the DNA is treated in a suitable buffer for at least 15 minutes at 15'C with 10 units of of the Klenow fragment of DNA polymerise I (Klenow) in the presence of the four deoxynudeotide triphosphates. The DNA is then purified by phenol-chloroform extraction and ethanol predptatan.
The deaved DNA fragments may be size-separated and selected using DNA gel electrophoresis. The DNA may be electrophoresed through either an agarose or a polyacrylamide matrix. The selection of the matrix will depend on the size of the DNA fragments to be separated. After electrophoresis, the DNA is extracted from the matrix by electroelutan, or, if low-melting agarose has been used as the matrix, by melting the agarose and extracting the DNA from it, as described in sections 6.30-6.33 of Sambrook et aL, supra.
The DNA fragments that are to be ligated together (previously digested with the appropriate restriction enzymes such that the ends of each fragment to be ligated are compatible) are put in solution in about equimolar amounts. The solution will also contain ATP, ligase buffer and a ligase such as T4 DNA ligase at about 10 units per 0.5 ug of DNA. If the DNA fragment is to be ligated into a vector, the vector is at first linearized by , cutting with the appropriate restriction endonudease(s). The linearized vector is then treated with alkaline phosphatase or calf intestinal phosphatase. The phosphatasing prevents self-ligation of the vector during the Iigation step.
After ligation, the vector with the foreign gene now inserted is transformed into a suitable host cell.
Prokaryotes are the preferred host cells for this invention. Suitable prokaryotic host cells inducts E. colt strain JM101, E. colt K12 strain 294 (ATCC number 31,446), E. colt strain W3110 (ATCC
number 27,325), E. colt X1776 (ATCC number 31,537), E. colt XL-1 Blue (stratagene), and E colt B;
however many other strains of E.
colt, such as H8101, NM522, NM538, NM539, and many other species and genera of prokaryotes may be used as well. In addition to the E. aoli strains listed above, badlli such as ~(~j~, other enterobacteriaceae such as m a and various p,~speaes may all be used as hosts.
Transformation of prokaryotic cells is readily accomplished using the caldum chloride method as described in section 1.82 of Sambrook et al., supra. Alternatively, electroporation (Neumann etaL, EMBO J..J..
1:841 [1982J) may be used to transform these cells. The transformed cells are selected by growth on an antit~iotic, commonly tetracydine (tet) or ampidllin (amp), to which they are rendered resistant due to the presence of tet and/or amp resistance genes on the vector.
After selection of the transformed cells, these cells are grown in culture and the plasmid DNA (or other vector with the foreign gene inserted) is then isolated. Plasmid DNA can be isolated using methods known in the art. Two suitable methods are the small scale preparation of DNA and the large-scale preparation of DNA as described in sections 1.25-1.33 of Sambrook et al., supra. The isolated DNA
can be purified by methods known in the art such as that described in section 1.40 of Sambrook etal., supra. This purified plasmid DNA is then analyzed by restriction mapping andlor DNA sequendng. DNA sequendng is generally pertormed by either the method of Messing et al. ~gg3" 9:309 [1981 J or by the method of Maxim et aL
~Qg~pty~j" 65:
ass [ls6oJ.
!V.
This invention contemplates fusing the gene erxlosing the desired polypeptide (gene t ) to a second gene (gene 2) such that a fusion protein is generated during transcription. Gene 2 is typically a coat protein gene of a phage, and preferably it is the phage M13 gene III coat protein, or a fragment thereof. Fusan of genes t and 2 may 5 be accomplished by inserting gene 2 inb a parGaa~aar site on a plasmid that contains gene 1, or by inserting gene 1 into a particular site on a plasmid that contains gene 2.
Insertion of a gene into a ptasmid requires that the plasmid be cut at the precise locatan that the gene is to be inserted. Thus, there must be a restriction erxionudease sibs at this bcation (preferably a unique site such that the plasmid will only be cut at a single location during restriction endorx~dease digestion). The plasmid is 10 digested, phosphatased, and purified as described above. The gene is then inserted into this linearized plasmid by ligatirg the two DNAs together. Ligatan can be accomplished if the ends of the plasmid are compatible with the ends of the gene to be inserted. If the restriction enzymes are used to cut the plasmid and isolate the gene to be inserted create blunt ends or compatible sticky ends, the DNAs can be ligated together directly using a ligase such as bacteriophage T4 DNA ligase and irxubating the mixture at 16'C for t ~4 hours in the presence of ATP
15 and Ngase buffer as described in section 1.68 of Sambrook et aL, ~. If the ends are rat compatible, they must first be made Bunt by using the Klenow fragment of DNA polymerase I or bacteriophage T4 DNA polymerase, both of which require the four deoxyribonudeotide triphosphates to fill-in overhanging single-stranded ends of the digested DNA Alternatively, the ends may be Bunted using a nuclease such as nuclease S1 a mung-bean rn~clease, both of which function by cutting back the overtranging single strands of DNA. The DNA is then 2 0 religated using a ligase as described above. In some cases, it may not be possible 6o Bunt the ends of the gene to be inserted, as the reading frame of the coding region will be altered. To overcome this problem, o6gonuGeotide linkers may be used. The linkers serve as a bridge to connect the plasmid m the gene to be inserted. These linkers can be made synthetically as double stranded or single stranded DNA using standard methods. The linkers have one end that is compatible with the ends of the gene b be inserted; the IiNcers are first ligated to this gene using Igation methods described above. The other end of the linkers is desgned to be compatible with the plasmid for ligation. In designing the linkers, care must be taken to not destroy the reading frame of the gene to be inserted or the reading frame of the gene contained on the plasmid. In some cases, it may be necessary to design the linkers such that they code for part of an amino acrd, or such that they code fa one or more amino aads.
Between gene 1 and gene 2, DNA encoding a termination colon may be inserted, such termination colons are UAG( amber), UAA (odder) and UGA (opal). (Microbiology, Davis et al.
Harper l~ Row, New York,1980, pages 237, 245-47 and 274). The termination colon expressed in a wild type host cell results in the synthesis of the gene t protein product without the gene 2 protein attached. However, growth in a suppressor host cell results in the synthesis of detectable quantities of fused protein. Such suppressor host cells contain a tRNA
modified to insert an amino acrd in the termination colon position of the mRNA
thereby resulting in production of detectible amounts of the fusion protein. Such suppressor host cells are well known and described, such as E.coli suppressor strain (Bullock et al., BioTechnioues 5, 376-379 [i987)). Any acceptable method may be used to place such a termination colon into the mRNA erxxxiing the fusion polypeptide.
The suppressible colon may be inserted between the first gene erxoding a polypeptide, and a second gene encoding at least a portion of a phage coat protein. Alternatively, the suppressible termination colon may be WO 92/09690 PCl'/US91/09133 2~~~5~~ ,s inserted adjacent to the fusion site by replacing the last amino acid triplet in the polypeptide or the first amino acrd in the phage coat protein. When the phagemid containing ttre suppressible colon is grown in a suppressor host cell, it results in the detectable production of a tusan polypeptide containing the polypeptide and the coat protein. When the phagemid is grown in a non-suppressor host cell, the polypeptide is synthesized substantially without fusion to the phage coat protein due to termination at the inserted suppressible triplet encoding UAG, UAA, or UGA. In the non-suppressor cell the polypeptide is synthesized and secreted from the host cell due to the absence of the fused phage coat protein which otherwise anchored it to the host cell.
V.
Gene 1, erxoding the desired poiypeptide, may be altered at one or more selected colons. An alteration , 0 is defined as a substitution, deletion, or insertion of one or more colons in the gene encoding the polypeptide that results in a change in the amino acid sequence of the polypeptide as compared with the unaltered or native sequence of the same polypeptide. Preferably, the alterations will be by substitution of at least one amino acid with any other amino acid in one a more regions of the molecule. The alterations may be produced be a variety of methods knoHm in the art. These methods include but are not limited to oligonudeotide-mediated mutagenesis and cassette mutagerresis.
81~
Oligonucleotide -mediated mutagenesis is preferred method for preparing substitution, deletion, and insertion variants of gene 1. This techr~que is weA knorm in the art as described by Zoller et al. Nucleic Aads Res.
IQ: 6487504 [1987]. Briefly, gene 1 is altered by hybridizing an oligonuGeotide encoding the desired mutation to a DNA template, where the 0emplate is the single-stranded form of the plasmid containing the unaltered or native DNA sequence of gene t. After hybridization, a DNA polymerise is used to synthesize an entire second complementary strand of the template will thus incorporate the oligonudeotide primer, and will code for the selected alteration in gene 1.
Generally, oligonuGeotides of at least 25 nucleotides in length are used. An optimal oligonuGeotide will have 12 to 15 nuGeotides that are completely complementary to the template on either side of the nudeotide(s) coding for the mutation. This ensures that the oligonuGeotide will hybridize properly to the single-stranded DNA
template molecule. The digonudeotides are readily synthesized using techniques IQrowrr in the art such as chat described by Crea et al. Proc. Nat,. Acid. Sa. USA 75: 5765 [1978].
The DNA template can only be generated by those vectors that are either derived from bacteriophage M13 vectors (the commercially available M13mp18 and M13mp19 vectors are suitable), or those vectors that contain a single-stranded phage orgin of replication as described by Viera et aL x,",53: 3 [1987].
Thus, the DNA that is to be mutated must be inserted into one of these vectors in order to generate single-stranded template. Production of the single-stranded template is described in sections 4.21-4.41 of Sambrook et al., supra.
To alter the native DNA sequence, the oligonudeotide is hybridized to the single stranded template under suitable hybridization conditions. A DNA polymerizing enzyme, usually the Klenow fragment of DNA
polymerise I, is then added to synthesize the complementary strand of the template using the oligonucleotide as a primer for synthesis. A heteroduplex molecule is thus formed such that one strand of DNA encodes the mutated form of gene 1, and the other strand (the original template) encodes the native, unaltered sequence of gene t .
WO 92/09690 ~ ~ !~ ~ PCT/US91/09133 This heterodupex molecule is then transformed into a suitable host cell, usually a prokaryote such as E. Coil JM101. After growing the cells, they are plated onto agarose plates and screened using the oGgonudeotide primer radiolabelled with 32-Phosphate to identify the bacterial colonies that contain the mutated DNA.
The method described immediately above may be modified such U~at a homoduplex molecule is created wherein both strands of the plasmid contain the mutation(s). The modifications are as follows: The single-stranded oGgonucleotide is ar~aied to the single-stranded template as described above. A mixture of three deoxyribonudeotides, deoxyriboadenosine (dATP), deoxyriboguanosine (dGTP), and deoxyribohymidine (dTTP), is combined with a modified thio~deoxyribocytosine called dCTP-(aS) (which can be obtained from Amersham).
This mixture is added to the template-o~gonudeotide complex. Upon addition of DNA polymerise to this mixture, a strand of DNA identical bo the template except for the mutated bases is generated. h addition, this new strand of DNA will contain dCTP-(aS) instead of dCTP, which serves to protect it from restriction endonudease digestion. After the template strand of the double-stranded heteroduplex is nicked with an appropriate restriction enzyme, the template strand can be digested with Exolll nudease or another appropriate nuGease past the region that contains the sites) to be mutagenized. The reaction is then stopped to leave a molecule that is only partially single-stranded. A complete double-stranded DNA homoduplex is then formed using DNA
polymerise in the presence of all lour deoxyribonudeotide triphosphates, ATP, and DNA Ggase. This homoduplex molecule can then be transformed hto a suitade host cell such as E. colt JM101, as described above:
Mutants witty more than one amino acrd to be substituted may be generated in one of several ways. 1f the amino aids are boated dose together h the pdypeptide chain, they may be mutated simultaneously u~rg one 2 0 oligonudeotide that codes for all of the desired amino acrd substitutions.
If, however, the amino acids are located some distance from each other (separated by more than about ten amino acids), it is more difficult to generate a single digonudeotide that encodes all of the desired changes. Instead, one of two alternative methods may be employed.
In the first method, a separate oligonudeotide is generated for each amino add to be substituted. The oligonudeotides are then amealed to the single-strarxJed template DNA
simultaneously, and the second strand of DNA that is synthesized from Use template wil encode all of the desired amino add substitutions. The alternative method involves two or more rounds of mutagenesis to produce the desired mutant. The first round is as described for the single mutants: wild-type DNA is used for the template, an oliganudeotide encoding the first desired amino acid substitutions) is annealed to this template, and the heteroduplex DNA molecule is then generated. The second round of mutagenesis utilizes the mutated DNA produced in the first round of mutagenesis as the template. Thus, this template already contains one or more mutations. The oligonucleotide encoding the additional desired amino acid substitutions) is then annealed to this template, and the resulting strand of DNA now encodes mutations from both the first and second rounds of mutagenesis. This resultant DNA
can be used as a template in a third round of mutagenesis, and so on.
B.
This method is also a preferred method for preparing substitution, delet'ron, and insertan variants of gene 1. The method is based on that described by Wells et at. ~,, 34:315 [1985].. The starting material is the plasmid (or other vector) comprising gene 1, the gene to be mutated. The codon(s) in gene 1 to be mutated are identified. There must be a unique restriction endorwdease site on each side of the identified mutation site(s). If ~' '~" f a 18 ~~.~~..~ a.~~~
no such restriction sites exist, they may be generated using the above~iesaibed olgonudeotide-mediated mutagenesis method to introduce them at appropriate locations in gene 1. After the restriction sites have been introduced into the plasmid, the plasmid is cut at these sires to linearize it. A double-stranded oligonudeotide encoding the sequence of the DNA between the restriction sites but containing the desired mutations) is synthesized using standard prooe~res. The two strands are synthesized separately and then hybridized together using standard techniques. This double-stranded oligonudeotide is referred to as the cassette. This cassette is designed to have 3' and 5' ends that are compatide with the ends of the linearized plasmid, such that it can be directly ligated 1o the plasmid. This ptasmid now contains the muhated DNA sequence of gene 1.
VI.
In an altemadve embodiment, this invention contemplates production of variants of a desired protein containing one or more subunits. Each subur~t is typically encoded by separate gene. F~ch gene encoding each subunit can be obtained by methods knovm in the art (see, for example, Section II). In some instances, it may be necessary to obtain the gene encoding the various subunits using separate techniques selected from any of the methods described in Section II.
When constmcting a replicable expression vector where the protein of interest contains more than one subunit, all subunits can be regulated by the same promoter, typically located 5' to the DNA encoding the subunits, or each may be regulated by separate promoter suitably oriented in the vector so that each promoter is operably linked to the DNA it is intended to regulate . Selection of promoters is carried out as described in Section III
above.
In constructing a repNcade expression vector aoMaining DNA encoding the protein of interest having multiple subunits, the reader is referred to Figure 10 where, by way of illustration, a vector is diagrammed showing DNA encoding each subunit of an antibody fragment. This figure shows that, generally, one of the subunits of the protein of interest will be fused to a phage coat protein such as M13 gene III. This gene fusion generally will contain its own sgnal sequence. A separate gene encodes the other subunit or suburits, and it is 2 5 apparent that each subur~it generally has its own signal sequerxe. Fgure 10 also shows that a single promoter can regulate the expression of both subunits. Alternatively, each subunit may be independently regulated by a different promoter. The protein of interest subunit-phage coat protein fusion construct can be made as described in Section IV above.
When constructing a famtly of variants of the desired multi-subunit protein, DNA encoding each subunit 3 0 in the vector may mutated in one or more positions in each suburut. When multi-subunit antibody variants are constructed, preferred sites of mutagenesis correspond to colons encoding amino acid residues located in the complementarily-determining regions (CDR) of either the light chain, the heavy chain, or both chains. The CDRs are commonly referred to as the hypervariable regrons. Methods for mutagenizing DNA encoding each subunit of the protein of interest are conducted essentially as described in Section V
above.
VII.
Target proteins, such as receptors, may be isolated from natural sources or prepared by recomt~inant methods by procedures known in the art. By way of illustration, glycoprotein hormone receptors may be prepared by the technique described by McFarland et al., 245:494-499 [1989J, norglycosylated forms expressed WO 92/09690 ~ ~ ~ ~, ~ ~ PCT/US91 /09I 33 in E. colt are described by Fuh et al. J. Biol. Chem 265:3111-3115 [1990]
Otter receptors can be prepared by standard methods.
The purified target protein may be attached to a suitable matrix such as agarose beads, acrylamide beads, glass beads, cellulose, various acxytic copdymers, hydroxylalkyl methaaylate gels, polyaaylic and polymethacrylic copolymers, nylon, neutral and ionic carriers, and the ike.
Attachment of the target protein to the matrix may be accomplished by methods described in ~p~p~, 44 (1976j, or by other means known in the art.
After attachment of the target protein to the matrix, the immobilized target is contacted with the library of phagemid particles under conditions suitable for binding of at least a portan of tf~e phagemid particles with the immobilized target. Nortnaliy, the conditions, including pH, ionic strength, temperature and the like will mimic physiological conditions.
Bound phagemid particles ('binders') having high affinity br the immobilized target are separated from those having a low affinity (and thus do not hind to the target) by washing. Binders may be dissociated from the immot~ilized target by a variety of methods. These methods include competitive dissodation using the wild-type ligand, altering pH arKilor ionic strength, and methods known in the art.
Suitable host cells are infected with the hinders and helper phage, and the host cells are cultured under conditans stable for amplification of the phagemid particles. The phagemid particles are then collected and the selection process is repeated one or more times unto hinders having the desired affinity for the target molecule are selected.
Optionally the library of phagemid particles may be sequentially contacted with more than one immolHlized target to improve selectivity for a particular target. For example, it is often the case that a ligand such as hGH has more than one r~at~al receptor. h the case of hGH, both the growth hormone receptor and tte prolaetin receptor bind the hGH ligand. ft may be desirable to improve the selectivity of hGH for the growth hormone receptor over the prolactin receptor. This can be achieved by ffrst contacting the library of phagemid particles with immot~ilized prolactin receptor, eluting those with a tow affinity (i.e. lower than wild type hGH) for tt~e prolactin receptor and then contacting the bw affinity prolactin 'binders' or non-hinders with the immobilized growth hormone receptor, and selecting for high affinity growth hormone receptor binders. In this case an hGH mutant having a lower affinity for the prolactin receptor would have therapeutic utility even if the affhity for the growth hormone receptor were somewhat lower than that of wild type hGH. This same strategy may be employed to improve selectivity of a particular hormone or protein for its primary function receptor over its clearance receptor.
In another embodiment of tfys invention, an improved substrate amino acid sequence can be obtained.
These may be useful for making better 'cut sites' for protein linkers, or for better protease substratesl~r~ihitors. h this embodiment, an immobitizable molea~e (e.g. hGH-receptor, biotin-avidin, or one capable of covalent linkage with a matrix) is fused to gene III through a linker. The linker will preferably be from 3 to 10 amino aads h length and will act as a substrate for a protease. A
phagemid wiU be constructed as described above where the DNA encoding the linker region is randomly mutated to produce a randomized library of phagemid particles with different amino acid sequences at the linking site. The library of phagemid particles are then immobilized on a matrix and exposed to a desired protease. Phagemid particles having preferred or better substrate amira acld ~ In the liner region kx the desired protease wAi be ekrted, fret pradudrtp an enriched pod of P~9em'd P~des er~adl~p prererred linkers. these phapemid tides are then cycled several mare limes lo t~ epos of particles an~n9 tense seqtrsnve(s) (~ epos Xlll and XIV~.
lltlt. ~Ge.YHdiIO~.A~d~, The domed gene for trGH rtes teen expressed ~ a seaetad tons in Fteals (G~4, C. rb, et al., (1987) hem ~5..i 89) and as oNn and amino add sequsnpa h~ been reported (Goeddel. er ~. [1 s79) ~Q.1.
544; Gray et af., l19&5] ~33~ 247?. The present inversion desaibss navel hGH
variants Prad~d u~n9 die p~~;d ~~pn methods. Human growth hormone verierss ~ fak p'°~~ns 10,14,18, 21, 187,171,172. l 74, 175,176,178 and 179 have been drd~ T~~ ~~rD trigher txndlng affinities are 10 deSCa'Ib6d ~ Tables VII, Xllt irld KiV. The amino acid rl~erl~t~ ~r deSCl~irlp 1119 Var~r~ is flow.
Growth honronB variants may be admlrtlstsred aM ~ ~ ~ s~ ~ "~"~r t~'"~
h°n"one. The growth hormone variants of the present invention may be expressed in any recomt~inanl system which is capable of expressing dative or met hGH.
T~~utic i4rmulafions of hGH for therapeutic admiryistration are prepared for storage by mixing 15 hGH having the desired degr~ of purity with opnonal physialogicatly atxeptable Carriers, exGipients, or stabilizers (13~71nnlon's Pita(m$~C~,L$C1~ ~~~ ~~ A.. ~d., (19801., in ~ form of tyoptw6ied cake or a4uevug ~lutions. Acceptable carriers, exoit>ienls or stabilizers era nontoxic bo reripiants at the dosages and C9n0enbailOns em~l6yEd, and irSClllde bllflta5 ~ 88 pfb"~, a~dte, arid DI~IBr Organic acids; ~tibbxid3nt5 inGuding asoabic sad; kyw mo'lsatlar weight pass than about 10 residues) poiypeptides; proteins. s~h as serum 2 o altxJmin. gelatin. or Immmogk~Gns: hydrnph~ic polymers auc+r as t~PYmolndone; amino atdds s~d~ as glycir~e, glutamine, asparagina, arginine, or lysine: monosaccharides, dis~rides, and other cart#tydrates including glucose. rnamose, or dexuins; chelating agents such as EDTA; divaler>t metal ions such as zinc, cobalt or copper:
sugar altohofs such as rnarrstol or sorbital; salt~tortning txsunlerions such as sodium; anchor norikmic suriacranls such as Tween; Pluronies~r po>yefhytene gtycot (isEG). Fortnulatior>s of the present invention may additionally 25 contain a phartnaaeuticallY aooeptable.butter, amino add, bulking agent arrdror non-ionic siufactant. These include, for example, buffers. tdieiating agents, antibxidarlLS, preservatives, cosotvent5, arid the like; spetxtic examples 9t these could include, trimethylamakte salts ('iris butter"), and dtsot8txn sdetate. Ttte phapemids of the present Invention may ba used to produce qttantitiefi bf the htahl verlar115 free of the phage pr018m. To expra35s ttGH
variants free of the gene III portion Of tt~a fusion, pS0643 and derivatives can simply be grown in a rtorr 30 suppressor strain such as i6C9. In ttris case, the amber colon (TAG) leads to tetrnlr>ation of iralnslation, which yields tree homtlxte, vntllout the need for an independent DNA consinxtion.
The frGH variant is setxeted from the host and may be isdated from the collars med'nim.
One or mbre of the e(ght hGH amino adds F10, M14, HiB, ill. Rt87, D171, T175 and 1179 may be raped by any amino tad other than the one found in that position (n r~turs~y rig hGH
as ~dicated. Thereiore,1, 2, 35 3, 4, 5, ti, 7, a a0 t3 of the indicated amino acids, F10, M14, H18, H21, Rtfi7,17171, T175 and 1179, may he replaced by any or the other 19 amino acids out of the 20 amino acids listed below. tn a preferred embodunerlt, as eight listed amino acids are replaced by another amino aid. The most preferred eight amino acids to be substituted are indicated in Table XIV in Example XII.
*trarlemark 2, An,ho add nomr~cla~re.
Ala (A) Arg (R) Asn (N) Asp (D) Cys (C) Gln (0) Glu (E) p Giy (G) His (H) Ile (I) Leu (L) Lys (K) Met (M) Phe (F) Pro (P) Ser (S) Thr (T) Trp (W) Tyr (Y) Val (V) The one letter hGH variant nomendadxe first gives the hGH amino acrd deleted, for example glutamate 179; then the amino acid inserted; for example, serine; resulting in (E1795S).
EXAMPLES
Without further desaiptan, it is believed that one of ordinary skill in the art can, using the preceding description and illustrative examples, make and utilize the present invention to the fullest extent. The follov~ng working examples therefore speafically point out preferred embodiments of the present invention, and are not to 3 0 be construed as limiting in any way of the remainder of the disclosure.
EXAMPLE I
Plasrrdd Cor~uciJons and Preparation o1 Mali-phagerr~ld Parfides The plasmid phGH-Ml3glll (Fig.1 ), was oonstnxted from M13K077 and the hGH
producing plasmid, pB0473 (Cunningham, B. C., et aL , ~jg~, 243:1330-1336, [1989]). A synthetic oligonucleotide 5'-AGC
TGT-GGC-TTC-GGG'CCC-TTA-GCA-TTT-AAT-GCG-GTA-3' was used to introduce a unique Apal restriction sib (underlined) into p80473 after the final Phel9t colon of hGH.
The oligonudeotide 5'-TTC-ACA-AAC-GAA-GGGCCC-CTA-ATT-AAA-GCC-AGA-3' was used to introduce a unique Apal restriction site (underlined), and a GIut97-to-amber stop oodon (bold lettering) into M13K07 gene III. The oligonuGeotide 5'-CAA-TAA-TAA-CGG-~'C~ T-AGC-CAA-AAG-AAC-TGG-3' introduces a unique Nhel site (underlined) after the 3' end of the gene III coding sequence. The resulting 650 base pair (bp) Apal-Nhel fragment from the doubly mutated M13K07 gene III was doped into the large Apal-Nhel fragment of pB0473 to create the plasmid, pS0132. This fuses the carboxyl terminus of hGH (Phe191 ) to the Pro198 residue of the gene III protein with the insertan of a glydne residue errcoded from the Apal site and places the fusion protein under control of the E. coG
alkaline phosphatase (ptaA) promoter and stll secretion signal sequence (Chang, C. N., et al. , ~gpg, 55:189-196, [1987)). For indudble expression of the fusion protein in rich media, we replaced the phoA promoter with the lac promoter and operabr. A 138 by EcoRl-Xbal fragment containing the lac promoter, operator, and Cap binding site was produced by PCR of plasmid pUC119 using the oligonudeotides 5'-CACGACAGAATTCCCGACTGGAAA-3' and 5'-CTGTT TCTAGAGTGAAATTGTTA-3' that flank the desired lac sequences and introduce the EcoRl and Xbal restriction sites (underlined).
Ttws lac fragment was gel pur'rfied and ligated into the large EcbRl-X6al fragment of pS0132 to create the plasmid, phGH-Ml3glll. The sequences of all taibred DNA junctions were verified by the dideoxy sequerxe method (Sanger, F., et aL Proc. Nail. Acad.
~j,~,~,g, 74:5463-5467, [1977)). The R64A variant hGH phagemid was constructed as follows: the Nsil-Bglll mutated fragment of hGH (Cunninghamet al. supra ) erxading the Arg64 to Ala substitution (R64A) (Cunningham, B. C., Wells, J. A., ~jgp~, 244:1081-1085, [1989]) was Boned between the corresponding restriction sites in the phGH-Ml3glll plasmid (Fig. 1) to replace the wild-type hGH sequence. The R64A hGH
phagemid particles were propagated and titered as described bebw for the wild-type hGH-phagemid.
Plasmids were transformed into a male strain of E. cali (JM101 ) and selected on carbenidllin plates. A
single transformant was grown in 2 ml 2n medium for 4 h at 3TC and infected with 50 W of Mt3K07 helper phage. The infected culture was diluted into 30 ml 2YT, grown overnight, and phagemid particles were harvested by predpitation with polyethylene glycol (Vierra, J., Messing, J. ,153:3-11, (1987]).
Typical phagemid particle titers ranged from 2 to 5 x 1011 cfu/ml. The particles were purified to homogeneity by CsCI density oentrrfugation (Day, L.A. J. Mol. Biol., 3965-277, (1969]) to remove any fusion protein not attached to virions.
hsrnnOd>e~al Analyses Of hGH m the Futon Phape Rabbit polydonal antibodies to hGH were purified with protein A, and coated onto microtiter plates (Nuns) at a corxernration of 2 Irglml in 50 mM sodium carbonate buffer (pH 10) at 4'C for 16-20 hours. After washing in PBS containing 0.05% Tween 20, hGH or hGH-phagemid particles were senialty diluted from 2.0 -0.002 nM in buffer A (50 mM Tris (pH 7.5), 50 mM NaCI, 2 mM EDTA, 5 mglml bovine serum albumin, and 0.05%
Tween 20). After 2 hours at room temperature (rt), the plates were washed well and the indicated Mab (Cunninghamet al. supra ) was added at 1 Nglml in buffer A for 2 hours at rt.
Following washing, horseradish peroxidase conjugated goat anti-mouse IgG antibody was bound at rt for 1 hour.
After a final wash, the peroxidase activity was assayed with the substrate, o~phenylenediamine.
3 5 FxAMPLE IA
Coupling of the hGH Binding Rolein bo Pdyaaylamlde Beads and Binding Eryichments Oxirane polyacrylamide beads (Sigma) were conjugated to the purified extracellular domain of the hGH
receptor (hGHbp) (Fuh, G., etal., J. Biol. Chem., 265:3111-3115 (1990)) containing an extra cysteine residue introduced by site-directed mutagenesis at positron 237 that does not affect binding of hGH (J. Wells, WO 92/09690 ~ ~ ~~ PCT/US91 /09133 unpublished). The hGHbp was corrugated as recommended by the supplier to a level of 1.7 pmol hGHbplmg dry oxirane bead, as measured by binding of [tag hGH b the resin. Subsequendy, any unreacted oxirane groups were blocked with BSA and Tris. As a control for non-specific binding of phagemid particles, BSA was similarly coupled to the beads. Buffer for adsorption and washing contained 10 mM
Tris~HCl (pH 7.5),1 mM EDTA, 50 mM
NaG,1 mglml BSA, and 0.0296 Tween 20. Elution buffers contained wash buffer plus 200 nM hGH or 0.2 M
glyclne (pH 2.1). Parental phage M13K07 was mixed with hGH phagemid particles at a rata of nearly 3000:1 (original mixture) and tumded for 8-12 h with a 5 N.I aliquot (0.2 mg of aaylamide beads) of either absorbent in a 50 p.1 volume at room temperature. The beads were peueted by oen~ifugation and the supemate carefully removed. The beads were resuspended in Z00 W wash buffer and tumbled at room temperatrue for 4 hours (wash t). After a second wash (wash 2), the beads were eluted twice with 200 nM hGH for 6-10 hours each (eluate 1, eluate 2). The final elution was with a glyclne buffer (pH 2.1 ) for 4 hours to remove remaining hGH
phagemid particles (eluate 3). Each fraction was diluted appropriately in 2n media, mixed with fresh JM101, incubated at 3TC for 5 minutes, and plated with 3 ml of 2n soft agar an LB or LB carbeniclllin plates.
iD(AMPLE IV
Cor~nrcUan of hGH.phagemld ParOd~ wlth a Mbct~e of Gene 11 Products The gene III protein is composed of 410 residues divided into two domains that are separated by a flexible linker sequence (Armstrong, J., elal., FEES Lett..135:167-172, [1981)). The amino-terrnir~al domain is required for attachment to the pill of E. cori, while the carboxyl-terminal domain is imbedded in the phage coat and required for proper phage assembly (Crissman, J. W., Smith, G. P., Viroloav.
132:445-455. [1984]). The signal 2 0 sequerxe and amino-terminal domain of gene Ill was replaced with the stll signal and entire hGH gene (Chang et al.
supra) by fusion to residue 198 in the carboxyl-terminal domain of gene III
(Fig.1 ). The hGH~ene III fusion was placed under control of the lac promoterloperator in a plasmid (phGH-M13g111;
Fg. 1) containing the pBR322 ~-lactamase gene and Col Et replication origin, and the phage ft intergenic region. The vector can be easily mair><ained as a small plasmid vector by selection on carber~allin, which avoids relying on a functional gene III fusion for propagation. Alternatively, the plasmid can be ef6clently packaged into virions (called phagemid particles) by infection with helper phage such as M13K07 (Yera et al.. supra ) which avoids problems of phage assembly.
Phagemid infectivity titers based upon transduction to carbeniclllin resistance in this system varied from 2-5 x 1011colony forming units (cfu~ml. The titer of the M13K07 helper phage in these phagemid stocks is ~1010 plaque forming units (pfu)Iml.
lAfith this system we confirmed previous studies (Parmiey, Smith supra) that homogeneous expression of large proteins fused to gene III is deleterious to phage production (data not shown). For example, induction of the lac promoter in phGH-Ml3glll by addition of IPTG produced low phagemid titers.
Moreover, phagemid particles produced by oo-infection with M13K07 contairurg an amber mutation in gene III
gave very low phagemid titers (<t010 ciulml). We believed that multiple copies of the gene 111 fusion attached to the phagemid surface could lead to multiple point attachment (the 'chelate effect') of the fusion phage to the immobilized target protein.
Therefore to control the fusion protein copy number we limited transcription of the hGH-gene III fusion by culturing the plasmid in E. colt JM101 (iacl~) which contains a oonstitutively high level of the lac repressor protein.
The E. colt JM101 cultures containing phGH-Ml3glll were best propagated and infected with M13K07 in the absence of the lac operon inducer (IPTG); however, this system is flexible so that co~xpression of other gene III
2~~5~3.3 24 tusan proteins can be balanced. We estimate that about 10°~ of the phagemid particles contain one copy of the hGH gene III fusion protein from the ratio of the amount of hGH per virion (based on hGH immures-reactive material in CsCI gradient purified phagemid). Therefore, the titer of fusion phage displaying the hGH gene III fusion is about 2 - 5 x 1010hn1. This number is much greater than the titer of E. aoli (-108 to l0glml) in the culture from which they are derived. Thus, on average every E. ooli cell produces 10-100 copies of phage decorated with an hGH gene III fusion protein.
EXAMPLE V
Structural Inteprtty of the hGH~ene II Fusion Immunoblot analysis (Fg. 2) of the hGH~ene III phagemid show that hGH aoss-reactive material comigrates with phagemid particles in agarose gels. This indicates that the hGH is tightly assoclated with phagemid particles. The hGH-gene III fusion protein from the phagemid particles runs as a single immuno-stained band showing that there is little degradation of the hGH when it is attached to gene III. Hfild-type gene III protein is dearly present because about 25% of the phagemid particles are infectious.
This is comparable to specific infectivity estimates made for wild-type M13 phage that are similarly purified (by CsCI density gradients) and concentrations estimated by UV absorbance (Smith, G. P. supra and Parmley, Smith supra) Thus, both wild-type gene III and the hGH-gene III fusion proteins are displayed in the phage pool.
It was important to confirm that the tertiary structure of the displayed hGH
was maintained. in order to have confidence that results from Minding selections will translate to the native protein. We used monoclonal antibodies (blabs) to hGH bo evaluate the sUuctural integrity of the displayed hGH gene III fusion protein (Table I).
TABLE L Binding of Eight Different Monoclonal AnfIbodles (Mab"s) to hGH end hGH Phagemld Particles' IC50 (nM) blab hGH hGH-phagemid __________..___________._..._______.________________._______....._________...__ ____ 1 0.4 0.4 2 0.04 0.04 3 0.2 0.2 4 0.1 0.1 5 0.2 >2.0 6 0.07 0.2 7 0.1 0.1 8 0.1 0.1 'Values given represent ~e oorxentrationGH or hGH-phagemid particles (nM) of h to give half-maximal binding to the particular Mab. Standard errors in these measurements are typically at or below 30%
of the reported value.
See Materials and Methods for further details.
The epitopes on hGH for these blabs have been mapped (Cunringham et al..
supra) and Minding for 7 of 8 blabs requires that hGH be properly folded. The IC50 values for all blabs were equivalent to wild-type hGH
except for Mab 5 and 6 . Both blabs 5 and 6 are known to have tHnding determinants near the carboxyl-terminus of hGH which is blocked in the gene III fusion protein. The relative IC50 value for Mabt which reacts with Moth native and denatured hGH is urxhanged oompan;d to the confortnationally sensitive blabs 2-5, 7 and 8. Thus, Mab1 serves as a good internal control for any errors in matching the concentration of the hGH standard to that of the hGH~ene 111 fusion.
WO 92/09690 ~ ~ ~ ~ ~, ~ ~ PCT/US91/09133 EXAMPLE VI
Bin~ng Enrldnferwa on Raoepbor AtANty BeHds Previous workers (Partnley, Smith supra ; Scott, Smith supra; Cwirla et al.
supra; and DeNin et al.
5 supra) have fractionated phage by panning with streptavidin coated polystyrene petri dishes or miaotiter plates.
However, chromatographic systems would allow more efficient fractionation of phagemid particles displaying mutant proteins with different binding affinities. We chose non-porous oxirane beads (Sigma) to avoid trapping of phagemid particles in the chromatographic resin. Furthermore, these beads have a small partite size (1 N.m) to maximize the surface area to mass ratio. The extracellular domain of the hGH
receptor (hGHbp) (Fuh ef al. , 10 supra) containing a free cysteira residue was effiaently coupled m these beads and phagemid particles showed very low non-specific bin ding to beads coupled oMy to bovine serum albumin (Table II).
TABLE II.
15 Specific Binding of Hormone Phage to hGHbp-coated Beads Provides an Enrichment for hGH-phage over M13K07 Phage' Sample Absorbent$ Total pfu Total cfu Ratio (cfu/pfu) Enrichment~
20 Original mixturet 8.3 x 1011 2.9 x 1p8 3.5 x 10'4 (1) Supernatant BSA 7.4 x 1011 2.8 x 108 3.8 x 10'4 1.1 hGHbp 7.6 x 1011 3.3 x 108 4.3 x 10'4 1.2 Wash 1 BSA 1.1 x 1010 6.0 x 106 5.5 x 10'4 1.6 hGHbp 1.9 x 1010 1.7 x 107 8.9 x 10'4 2.5 25 Wash 2 BSA 5.9 x 107 2.8 x 104 4.7 x 10'4 1.3 hGHbp 4.9 x 107 2.7 x 106 5.5 x 10'2 1.6 x 102 Eluate 1 (hGH)BSA t .1 x 106 1.9 x 103 1,7 x 10'3 4.9 hGHbp 1.2 x 106 2.1 x 106 1.8 5.1 x 103 Eluate 2 (hGH)BSA 5.9 x 105 1.2 x 103 2.0 x 10'3 5.7 hGHbp 5.5 x 105 1.3 x 106 2.4 6.9 x 103 Eluate 3 (pH 2.1 )BSA4.6 x 105 2.0 x 103 4.3 x 10'3 12.3 hGHbp 3.8 x 105 4.0 x 106 10.5 3.0 x 104 'The titers of M13K07 and hGH-phagemid particles in each fraction was determined by multiplying the number of plaque forming units (pfu) or carbenicillin resistant colony forming units (cfu) by the dilution factor, respectively. See Example IV for details.
tThe ratio of M13K07 to hGH-phagemid particles was adjusted to 3000:1 in the original mixture.
$Absorbents were conjugated with BSA or hGHbp.
~Enrichments are calculated by dividing the cfu/pfu ratio after each step by cfu/pfu ratio in the original mixture.
In a typical enrichment experiment (Table II), one part of hGH phagemid was mixed with >3,000 parts M13K07 phage. After one cyGe of binding and elution,106 phage were recovered and the ratio of phagemid to M13K07 phage was 2 to 1. Thus, a single binding selection step gave >5000-~Id enrichment. Additional elutions with free hGH or acid treatment to remove remaining phagemids produced even greater enrichments. The enrichments are comparable to those obtained by Smith and coworkers using batch elution from coated polystyrene plates (Smith, G.P. supra and Parmely, Smith sypra ) however much smaller volumes are used on the WO 92/09690 N Q ~ ~ b ~y ~~ PCT/US91/09133 beads (200 W vs. 6 ml). There was almost no enrichment for the hGH phagemid over M13K07 when we used beads linked only to BSA. The slight enrictxnent observed for control beads (-10-fold for pH 2.t elution; Table 2) may result firom trace contaminants of bovine growth hormone t>;n<ling protein present in the BSA linked to the bead. Nevertheless these data show the enrichmer><s for the hGH phage depend ion the presence of the hGHbp on the bead suggesting Minding oxurs by specific interactan between hGH and the hGHbp.
We evaluated the enrichment for wild-type hGH over a weaker bir»dirg variant of the hGH on fusion phagemids to further demonstrate enrichment speafiaty, and to Nnk the reduction in binding affinity for the purffied hormones to ervichment factors after panrwng fusion phagemids. A
fusion phagemid was constmcted with an hGH mutant in which Arg64 was substituted with Ala (R64A). The R64A
variant hormone is about 20-fold reduced in receptor binding affinity compared to hGH (Kd values of 7.t nM
and 0.34 nM, respectively [Cunningham, Wells, supra )). The titers of the R64A hGHt~ene III fusan phagemid were comparable to those of wild-type hGH phagemid. After one round of binding and elution (Table III) the wild-type hGH phagemid was enriched from a mixture of the two phagemids plus M13K07 by 8-fold relative to the phagemid R64A, and 104 relative to M13K07 helper phage.
TABLE WI. hGHbp~ooated Beads Select fa hGH PhaperNds Over a Weaker B4~np hGH Variant tfiapemld Sample enrichment j~ enrichment total phagemid for WT/R64A total phagemid for VIrT/R6~4A
Original mixture 8/20 (1) 8/20 (1) Supernatant ND - 4/10 1.0 Elution 1 (hGH) 7J20 0.8 17120 8.5$
Elution 2 (pH 2.1 ) 11 /20 1.8 21 /27 5.2 'The parent M13K07 phage, wild-type hGH phagemid and R64A pt~agemid particles were mixed at a ratio of 104:0.4.6. Binding selections were carried out using beads linked with BSA
(control beads) or with the hGHbp (hGHbp beads) as described in Table II and the Materials and Methods After each step, plasmid DNA was isolated(&mboim, H. C., Doly, J. , Nucleic Acids Res., T:1513-1523, [1979]) from carbeniallin resistant colonies and analyzed by restriction analysis to determine if it contained the wild-type hGH or the R64A hGH gene III
fusion.
tThe enrichment for wild-type hGH phagemid over R64A mutant was ca~ulated from the rata of hGH phagemid present after each step to that present in the original mixture (8120), divided by the coresponding ratio for R64A phagemids. WT = wild-type; ND = not determined.
$The enrichment for phagemid over total M13K07 parental phage was -104 after this step.
4 0 By displaying a mixture of wild-type gene III and the gene 111 fusion protein on phagemid particles one can assemble and propagate virions that display a large and proper folded protein as a fusion to gene III. The copy number of the gene III fusion protein can be effectively controlled to avoid'chelate effects' yet maintained at high enough levels in the phagemid pool to permit panning of large epitope libraries (>1010). We have shown that hGH
(a 22 kD protein) can be displayed in its native folded torm. Binding selections performed on receptor affinity beads eluted with free hGH, efficiently enriched for wild-type hGH phagemids over a mutant hGH phagemid shown to gave reduced receptor binding affinity. Thus, it is possible to sort phagemid particles whose binding constants are doom in the r~anomolar range.
WO 92/09690 ~ 9 j ~ 3 ~ PCT/US91 /09133 Proteirrprotein and antibody-antigen interactions are dominated by discontinuous epitopes (Janin, J., et al. , J.J. MoLBiol..Biol.. 204:155-164, (1988]; Argos, P., Prot Ena., 2a01-113, (19881; Barlow, D.J.,etaL , ~,, 322:747-748, [1987); and Davies, D.R., et at. , ,(,,Biol. Chem.. 263:10541-10544, [1988)); that is the residues directly involved in binding are dose in tertiary structure but separated by residues not involved in binding. The screening system presented here should albw one to analyze more conveniently protein-receptor interactions and isolate disoont~uous epitopes in proteins with new and high affinity binding properties.
S~don d hGH from a IJbrary Rundomtaed at hGH Colons 172,174,176,178 i 0 Constructi~~n of template A mutant of the hGH~ene III fusion protein was constructed using the method of Kunkel.,et aL fuj~.
Fp~,154, 367-382 [1987]. Template DNA was prepared by growing the plasmid pS0132 (containing the natural hGH gene fused to the carboxy-terminal half of M13 gene III, under control of the alkaline phosphatase promoter) in CJ236 cells with M13-K07 phage added as helper. Single-stranded, uradl-containing DNA was prepared for mutagenesis to introduce (1) a mutation in hGH which would greatly reduce binding to the hGH
binding protein (hGHbp); and (2) a unique restriction site (Kpnl) which could be used for assaying for -- and selecting against - parental background phage. Oligonudeotide-directed mutagenesis was carried out using T7 DNA polymerise and the foNowing digodeoxy-nucleotide:
Gly Thr hGH colon: 178 179 5' -G ACA TTC CTG S-aGT A~.C GTG CAG T-3' < KpnI >
This oligo introduces the Kpnl site as shown, along with mutations (R178G,1179T) ~ hGH. These mutations are predicted to reduce binding of hGH to hGHbp by more than 30-fold. Clones from the mutagenesis were screened by Kpnl digestion and confirmed by dideoxy DNA sequencing. The resulting constrict, b be used as a template for random mutagenesis, was designated pH04t5.
~ ~ggpn Ihel6c~l of hCt( Colons 172,174,176,178 were targeted for random mutagenesis in hGH, again using the method of Kunkel. Single-strarxied template from pH0415 was prepared as above and mutagenesis was carried ouf using 3 0 the following pool of oligos:
hGH colon: 172 174 5'- GC TTC AGG AAG GAC ATG GAC ~ GTC )~ ACA-Ile .5. CTG ~ ATC GTG CAG TGC CGC TCT GTG G-3' As shown, this oligo pool reverts colon 179 to wtid-type (tie), destroys the unique Kpnl site of pH0415, and introduces random colons (NNS, where N= A,G,C, or T and S= G or C) at positions 172,174,176, and 178. Using this colon selection in the context of the above sequence, no additional Kpnl sites can be seated. The choice of the NNS degenerate sequence yields 32 possible colons (inducting one 'stop' colon, and at least one colon for each amino add) at 4 sites, for a total of (32)4= 1,048,576 possible nudeotide sequences (12°~ of which contain at least one stop colon), or (20)4= 160,000 possible polypeptide sequences plus 34,481 prematurely terminated sequences (i.e. sequerxes contair>ing at least one stop colon).
PSg~,~geffon of tnlfjat Ilbrarv WO 92/09690 ~ ~ ~ C~ ~ J ~ PCT/US91/09133 The mutagenesis products were extracted twice with phenolxtrloroform (50:50) and ethanol precpitated with an excess of carrier tRNA 6o avoid adding salt that would confound the subsequent electroporation step. Approximately 50 ng (15 fmols) of DNA was electroporated into WJM101 cells (2.8 x 101 ~
oeIIsImL) in 45 N.L btal volume in a 0.2 an cuvette at a voltage setting of 2.49 kV with a single pulse (time constant = 4.7 msec.).
The ceNs were aNowed to recover 1 hour at 37oC with shaking, then mixed with 25 mL 2YT medium,100 ~glmL carbenicllin, and M13-K07 (multiplicity of infection = 1000). Plating of serial dilutions from this culture onto carbenialGn~onta~ing media indicated that 8.2 x 106 electrotransformarris were obtained. After 10' at 23oC, the culture was incubated overnight (15 hours) at 37oC with shaking.
After ovemght incubation, the cells were pelleted, and double-stranded DNA
(dsDNA), designated pLIB1, was prepared by the alkaline lysis method. The supernatant was spun again to remove any remaining cells, and the phage, designated phage pool ~1, were PEG-precipitated and resuspended in 1 mL STE buffer (10 mM
Tris, pH 7.6, 1 mM EDTA, 50 mM NaCI). Phage titers were measured as colony-formirg units (CFU) for the recombinant phagemid containing hGH~3p gene III fusion (hGH~) plasmid, and plaque-forming units (PFU) for 1 S the M13-K07 helper phage.
1. BINDING: M aliquot of phage pool ~1 (6 x 109 CFU, 6 x 107 PFU) was diluted 4.5-fold in buffer A
(Phosphate-buffered saline, 0.5% BSA, 0.05°~ Tween-20, 0.01°k thimerosal) and mixed with a 5 uL suspension of oxirane-polyacrylamide beads coupled to the hGHbp containing a Ser237 Cys mutation (350 fmols) in a 1.5 mL
silated polypropylene tube. As a control, an equivalent aNquot of phage were mixed in a separate tube with beads that had been coated with BSA only. The phage were allowed to hind to the beads by incubating 3 hours at room temperature (23oC) with slow rotation (approximately 7 RPM). Subsequent steps were carried out with a constant volume of 200~L and at room temperature.
2. WASH: The beads were spun 15 sec., and the supernatant was removed (Sup.1 ). To remove phage/phagemid not specifically bound, the beads were washed twice by resuspending in buffer A, then pelleting.
A final wash consisted of rotating the beads in buffer A for 2 tours.
3. hGH ELUTION: Phagelphagemid Minding weakly to the beads were removed by stepwise elution with hGH. In the first step, the beads were rotated with buffer A containing 2 nM
hGH. After 17 hours , the beads were pelleted and resuspended in buffer A containing 20 nM hGH and rotated for 3 hours, then pelleted. In the 3 0 final hGH wash, the beads were suspended in buffer A containing 200 nM hGH
and rotated for 3 tours then pelleted.
4. GLYCINE ELUTION: To remove the tightest-binding phagemid (i.e. those still bound after the hGH
washes), beads were suspended in Glycne buffer (1 ~Glycine, pH 2.0 with HCI), rotated 2 hours and pelleted.
The supernatant (fraction 'G'; 200~.L) was neutralized by adding 30 Nl. of 1 M
Tris base.
Fraction G eluted from the hGHbp-beads (1 x 106 CFU, 5 x 104 PFU) was not substantially enriched for phagemid over K07 helper phage. We believe this resulted from the fact that K07 phage packaged during propagation of the recombinant phagemid display the hGH-gap fusion.
WO 92/09690 ~ ~ 9 ~ ~ ~ ~ PCT/US91/09133 However, when compared with fraction G eluted from the BSAcoated control beads, the hGHbp-beads yielded 14 times as many CFU's. This reflects the enrichment of tight-binding hGH~displaying phagemid over nonspedfically-binding phagemid.
5. PROPAGATION: M aliquot (4.3 x 105 CFU) of fractan G ekited from the hGHbp-beads was used to infect log-phase WJM101 cells. Transductans were cartied out by mixing 100 N.L fractan G with 1 mL WJM101 cells, incubating 20 min. at 37oC, then adding K07 (multiplidty of infection=1000). Cultures (25 mL 2YT plus carberudllin) were grown as described above and the second pool of phage (Library 1G, for first glydne elution) were prepared as described above.
Phage from library 1 G (Fig. 3) were selected for tlinding to hGHbp beads as described above. Fraction G eluted from hGHbp beads contained 30 times as many CFU's as fir~tion G
eluted from BSA-beads in this selectan. Again, an aliquot of fraction G was propagated in WJM101 cells to yield library 1G2 (indicating that this library had been twice selected by glyane elution). Double-stranded DNA
(pLIB 1 G2) was also prepared from this culture.
To reduce the level of background (Kpnl+) template, an aliquot (about 0.5 p.g) of pLIB 1G2 was digested with Kpnl and electroporated into WJM101 cells. These cells were grown in the presence of K07 (multiplidty of infection= i00) as described for the initial library, and a new phage pool, pLIB 3, was prepared (Fig. 3).
In addition, an aliquot (about 0.5 fig) of dsDNA from the initial library (pLIB1) was digested with Kpnl and electroporated directly into WJM101 cells. Transfortnants were allowed to recover as above, infected with M13-K07, and grown overnight to obtain a new Gtxary of phage, designated phage Library 2 (Fig. 3).
Phagemid binding, elution, and propagation were carried out in successive rounds for phagemid derived from both pLIB 2 and pLIB 3 (Fig. 3) as described above, except that (1) an excess (10-fold over CFU) of p~xified K07 pfrage (not ~sptaying hGH) was added in the bead-binding cocktail, and (2) the hGH stepwise elutions were n;plaoed with txief washings of buffer A alone. Also, in some cases, XL1-Blue cells were used for phagemid propagation.
An additional digestion of dsDNA with Kpnl was carried out on pLIB 2G3 and on pLlB 3G5 before the final round of bead-binding selection (Fg. 3).
Four independently isolated doves from LIB 4G4 and bur indeperxiently isolated doves from LIB 5G6 were sequenced by dideoxy sequendng. All eight of these doves had identical DNA sequences:
hGH colon: 172 174 176 178 5' -AAG GTC TCC ACA TAC CTG AGG ATC-3' Thus, all these encode the same mutant of hGH: (E174S, F176Y). Residue 172 in these Bones is Lys as in wild-type. The colon selected for 172 is also identical to wild-type hGH. This is not surprising since AAG is the only lysine~odon possible from a degenerate 'NNS' colon set. Residue 178-Arg is also the same as wild-type, but here, the colon selected from the library was AAG instead of CGC as is found in wild-type hGH, even though the latter colon is also possible using the'NNS' colon set.
p 30 The multipliclty of infection of K07 infection is an important parameter in the propagation of recomtHnant phagemids. The K07 multiplidty of infection must be high enough to insure that virtually all cells transformed or transfected with phagemid are able to package new phagemid particles. Furthermore, the concentration of wild-type gene III in each cell should be kept high Eo reduce the possibility of multiple hGH-gene III
fusion molecules being displayed on each phagemid particle, thereby reduarg chelate effects in binding. However, if the K07 multiplidty of infection is too high, the packaging of K07 will compete with that of recombinant phagemid. We find that aooeptabfe phagemid yields, vhth oMy 1-10% badc~ound K07 phage, are obtained when the K07 mutGplidty of infection is 100.
Table IV.
Phage Pool moi (K07) Enrichment hGHbpIBSA beads Fraction Kpnl CFUIPFU
LIB 1 1000 ND 14 0.44 LIB 1G 1000 ND 30 0.57 LIB 3 100 ND 1.7 0.26 LIB 3G3 10 ND 8.5 0.18 LIB 3G4 100 460 220 0.13 LIB 2 100 ND 1.7 <0.05 LIB 2G 10 ND 4.1 <0.10 LIB 2G2 100 1000 27 0.18 Phage pools are labelled as shown (Fig. 3). The multiplidty of infection (moi) refers to the multiplidty of K07 infection (PFUlcells) in the propagation of ptragemid. The enrictunent of CFU
over PFU is shown in those cases where purified K07 was added in the binding step. The rata of CFU eluting from hGHbp-beads over CFU eluting from BSA-beads is shown. The fraction of Kpnl-containing template (i.e., pH0415) remaining in the pool was determined by digesting dsDNA with Kpnl plus EcoRl, running the products on a 1°k agarose gel, and laser-scanning a negative of the ethidium bromide-stained DNA.
R~'gp~tor-blndina aftlnHp~r of fhe hormone hGH(E174S. F176Y1 The fact that a single done was isolated from iwo different pathways of selection (Fig. 3) suggested that the double mutant (E174S,F176Y) hinds strongly to hGHbp. To determine the affinity of this mutant of hGH for hGHbp, we constructed this mutant of hGH by site~irected mutagenesis, using a plasmid (pB0720) which contains the wild-type hGH gene as template and the following oligonuGeotide which changes colons 174 and hGH colon: 172 174 176 178 Lys Ser Tyr Arg 5'- ATG GAC AAG GTR ~G ACA T8C CTG CGC ATC GTG -3' The resulting construct, pH0458B, was transformed into E. coli strain 16C9 for expression of the mutant hormone. Scatchard analysis of competitive tHnding of hGH(E174S,F176Y) versus 1251-hGH to hGHbp indicated that the (E174S,F176Y) mutant has a Minding affinity at least 5.0-fold tighter than that of wild-type hGH.
EXAMPLE VUI
SELECTION OF hGH VAFBANTS FROM A
Human growth hormone variants were produced by the method of the present irnention using the phagemid desaibed in figure 9.
We designed a vector for cassette mutagenesis (Wells et al., ~, 34, 315-323 [1985)) and expression of the hGH-gene III fusion probin with the objectives of (1 ) improving the wnkage between hGH and the gene I II
moiety to more favorably display the hGH moiety on the phage (2) limiting expression of the fusion protein to obtain essentially 'monovaleM display,' (3) allowing for restriction rxidease selection against the starting vector, (4) eliminating expression of fusion protein from the starting vector, and (5) achieving taale expression of the corresponding free hormone from a given hGH-gene III fusion mutant.
Plasmid pS0643 was constructed by oligonuGeotide-directed mutagenesis (Kunkel et al., Fp~,154, 367-382 [1987J) of pS0132, which contains pBR322 and fi origins of repbcation and expresses an hGH-gene III fusion protein (hGH residues 1-191, followed by a single Gly residue, fused to Pro-198 of gene III) under the control of the F,,~ p(ZQ6 promoter (Bass et al., Proteins 8, 309-314 [1990])(Figure 9). Mutagenesis was carried out with the oligonuGeotide 5'-GGC-AGC-TGT-GGC-TT_r,~AG-AGT-GGC-GGC-GGC-TCT-GGT-3', which introduces a ~[ site (underlined) and an amber stop colon (TAG) foNowing Phe-19t of hGH. In the resulting construct, pS0643, a portion of gene III was deleted, and two silent mutations (underlined) occurred, yielding the folbwing junction between hGH and gene III:
__ ~ _______.__________.> geae » >
2 5 1B7 188 18B 190 181 am' 249 2!!0 261 2:3~ 2S3 264 t9Q Gars G19 P>'e (;fin tsa Cdr G~ Gf;Y ~' ~9 GGC AOC TGT GGA ThC TAG ~IGT O0~ (ifs'T' C1GC TCT GiG1' This shortens the total size of tire fusion protein firom 401 residues ~
pS0132 to 350 residues in 3 0 pS0643. Experiments using monoclonal antibodies against hGH have demonstrated that the hGH portion of the new fusion protein, assemded on a phage particle, is more acoesside than was the previous, longer fusion.
For propagation of hormone-displaying phage, pS0643 and derivatives can be grown in a amber-suppressor strain of ~, such as JM101 or XL1-Blue (Bullock et al., BioTecbpj~
5, 376-379 [1987j). Shown above is substitution of Glu at the amt~er colon which occurs in ;~ suppressor strains. Suppression with other 3 5 amino acids is also possible in various available sUair~s of ~,,~ well known and publicalty available.
To express hGH (or mutants) tree of the gene III portion of the fusion, pS0643 and derivatives can simply be groHm in a non-suppressor strain such as 16C9. ~ ihis case, the amber colon (TAG) leads to termination of translation, which yields free hormone, without the need for an independent DNA construction.
To create sites for cassette mutagenesis, pS0643 was mutated with the oligonucJeotides (1 ) 5'-CGG-40 ACT-GGG-CAG-ATA-TTC-AAG-CAG-ACC-3', which destroys the unique $q(1[ site of pS0643; (2) 5'-CTC-AAG-AAC-TAC-GGG-TTA-CCC-TGA-CTG-CTT-CAG-GAA-GG-3', which inserts a unique ~j site, a single-base (rameshift, and a non-amber stop colon (TGA); and (3) 5'-CGC-ATC-GTG-CAG-TGC-AGA-TCT-GTG-GAG-GGC-3', which introduces a new ~ site, to yield the starting vector, pH0509. The addition of a frameshift along with a TGA stop colon insures that no genelll-fusion can be produced from the startirg vector.
WO 92/09690 ~ Q ~ PCT/LJS91/09133 The ~[[ - ~[[1 segment is cut out of pH0509 and replaced with a DNA cassette, mutated at the colons of interest. Other restriction sites for cassette mutagenesis at other locatans in hGH have also been introduced into the hormone-phage vector.
Colons 172,174, 176 and 178 of hGH were targeted for random mutagenesis because they all lie on or near the surface of hGH and contribute significantly to receptor-binding (Cunningham and Wells, 244, 1081-1085 (1989]); they all we within a well-defined structure, occupying 2'tums' on the same side of helix 4;
and they are each substituted by at least one amino aad among krawn evolutionary variants of hGH.
We chose to s~stitute NNS (N=A/G/C/T; S=G/C) at each of the target residues.
The choice of the NNS degenerate sequence yields 32 possible codor~s (including at least one colon for each amino aad) at 4 sites, for a total of (32)4= 1,048,576 possible nucleotide sequences, or (20)4=
160,000 possible polypeptide sequences. Only one stop colon, amber (TAG), is albwed by this choice of colons, and this colon is suppressible as Glu in ~ strains of ~.
Two degenerate oligonudeotides, with NNS at colons 172,174,176, and 178, were synthesized, phosphorylated, and annealed to construct the mutagenic cassette: 5'-GT-TAC-TCT-ACT-GCT-TTC-AGG-AAG-GAC-ATG-GAC-NNS-GTC-NNS-ACA-NNS-CTG-NNS-ATC-GTG-CAG-TGC-A-3', and 5'-GA-TCT-GCA-CTG-CAC-GAT-SNN-CAG-SNN-TGT-SNN-GAC-SNN-GTC-CAT-GTC-CTT-CCT-GAA-GCA-GTA-GA-3'.
The vector was prepared by digesting pH0509 with followed by ~[[j. The products were run on a 1% agarose gel and the large fragment exased, phenol-extracted, and ethanol precipitated. This fragment was treated with calf intestinal phosphatase (Boehringer), then phenol:chloroform extracted, ethanol preapitated, and resuspended for ligatan with the mutagenic cassette.
rr~~umn uu: nnu~ nu~~r m w~rw~ ma Following ligation, the reaction products were again digested with , then phenol:chloroform extracted, ethanol precipitated and resuspended in water. (A g~j1 recognition site (GGTNACC) is created within cassettes which contain a ~ at position 3 of colon 172 and an ~ (Thr) colon at 174. However, treatment with ~stEll at this step should not select against any of the possible mutagenic cassettes, because virtually all cassettes will be heteroduplexes, which cannot be Geaved by the enzyme.) Approximately 150 ng (45 fmols) of DNA was electroporated into XL1-Blue cells (1.8 x 109 cells in 0.045 mL) in a 0.2 an cuvette at a voltage setting of 2.49 kV with a single pulse (time constant = 4.7 msec.).
The cells were allowed to recover 1 hour at 37oC in S.O.C meda with shaking, then mixed with 25 mL
2YT medium,100 mg/mL carbenicillin, and M13-K07 (moi= 100). After 10' at 23oC, the culture was incubated overnight (15 hours) at 37oC with shaking. Plating of serial dilutions from this culture onto carbenicillin-containing media indicated that 3.9 x 107 electrotransformants were obtained.
After overnight incubation, the cells were pelleted, and double-strarxied DNA
(dsDNA), designated pH0529E (the initial library), was prepared by the alkaline lysis method. The supernatant was spun again to remove any remaining cells, and the phage, designated phage pool ~H0529E (the initial lilxary of phage), were PEG-precipitated and resuspended in 1 mL STE buffer (10 mM Tris, pH 7.6, 1 mM
EDTA, 50 mM NaCI). Phage WO 92/09690 ~ j ~ ~ ~ PCT/US91/09133 titers were measured as cobny-forming units (CFU) for the recombinant phagemid containing hGH-gap.
Approximately 4.5 x 1013 CFU were obtained from the starting library.
From the pool of electrotransformants, 58 doves were sequenced in the region of the ~-gq(j[
cassette. Of these, 17% corresponded to the starting vector,17% contained at least one frame shift, and 7°~6 contained a non-silent (non-terminating) mutation outside the four target colons. We conclude that 41°~ of the doves were defective by one of the above measures, leaving a botat functional pool of 2.0 x 107 initial transfortnants. This number stiN exceeds the possible number of DNA sequences by nearly 20-fold. Therefore, we are confident of having all possible sequences represented in the starting litxary.
We examined the sequences of non-selected phage to evaluate the degree of colon bias in the mutager~esis (Table V). The results indicated that, although some colons (and amino adds) are under- or over-represented relative to the random expectation, the library is extremely diverse, with no evidence of large-scale 'sibling' degeneracy (Table VI).
Table V.
Colon distributan (per 188 colons) of non-selected hormone phage. Cbnes were sequenced from the starting library (pFt0529E). All colons were tabulated, inducting those from Bones which contained spurious mutations andlor frameshifts. ' Note: the amber stop colon (TAG) is suppressed as Glu in XLt-Blue cells. Highlighted colons were ovedunder-represented by 50% or more.
leu 17.6 18 1.0 Ser 17.6 26 1.5 A r g 17.6 10 0.57 Pro 11.8 16 1.4 Thr 11.8 14 1.2 Ala 11.8 13 1.1 Gly 11.8 16 1.4 Val 11.8 4 0.3 1e 5.9 2 0.3 Met 5.9 1 0.2 Ty r 5.9 1 0.2 Ws 5.9 2 0.3 Trp 5.9 2 0.3 Phe 5.9 5 0.9 4 Cys 5.9 5 0.9 Gln 5.9 7 1.2 Asn 5.9 14 2.4 Lys 5.9 11 1.9 Asp 5.9 9 1.5 Glu 5.9 6 1.0 amber' 5.9 6 1.0 WO 92/09690 ~ ~ ~ ~ ~ PCT/US91/09133 Table VI.
Non-selected (pH0529E) clones with an open reading frame.
The notation, e.g. TWGS, denotes the hGH mutant 172TI174WI176G/178S. Amber (TAG) colons, translated as GIu in XL1-Blue cells are shown as E.
Ke NT KTEQ CVLQ
TWGS NNCR EASL
Pe ER FPCL SSKE
LPPS NSDF ALLL
SLDP HRPS PSHP
OQSN LSLE SYAP
GSKT NGSK ASNG
TPVT LTTE EANN
RSRA PSGG KNAK
LCGL LWFP SRGK
TGRL PAGS GLDG
AKAS GRAK NDPI
GNDD GTNG
Immobilized hGHbp ('hGHbp-beads') was prepared as described (Bass et al., Proteins 8, 309-314 [1990]), except that wild-type hGHbp (Fuh et al., ~, Biol. Chem. 265, 3111-3115 [1990]) was used. Competitive binding experiments with [1251] hGH indicated that 58 fmols of turxtional hGHbp were coupled per uL of bead Immobilized hPRLbp ('hPRlbp-beads') was prepared as above, using the 211-residue extraoelluiar domain of the prolactin receptor (Cunningham et al., ~jg~ 250,1709-1712 [1990]). Competitive binding experiments with [1251] hGH in the presence of 50 l,t~ zinc indicated that 2.1 fmols of functional hPRLbp were 3 0 coupled per N.L of bead suspension.
'Blank beads' were prepared by treating the oxirane-acrylamide beads with 0.6 M etharalamine (pH
9.2) for 15 hours at 4oC.
Binding of hormone-phage to beads was carried out ~ one of the following buffers: Buffer A (PBS, 0.5% BSA, 0.05°~ Tween 20, 0.01°~ thimerosal) for selections using hGHbp and blank beads; Buffer B (50 mM
tris pH 7.5,10 mM MgCl2, 0.5% BSA, 0.05°~ Tween 20,100 mm ZnCl2) for selections using hPRLbp in the presence of zinc (+ Zn2+); or Buffer C (PBS, 0.5% BSA, 0.05% Tween 20, 0.01%
thimerosal, 10 m~ EDTA) for selections using hPRLbp in the absence of zinc (+ EDTA). Binding selections were carried out according to each of tt~e following paths: (1) binding to blank beads, (2) binding to hGHbp-beads, (3) binding to hPRLbp-beads (+
Zn2+), (4) binding to hPRLbp-beads (+ EDTA), (5) pre-adsorbing twice with hGHbp beads then binding the non-adsorbed fraction to hPRLbp-beads ('-hGHbp, +hPRLbp' selection), or (6) pre-adsorbing twice with hPRLbp-beads then binding the non-adsorbed fraction to hGHbp-beads ('-hPRLbp, +hGHbp' selection). The latter two procedures are expected to enrich for mutants binding hPRLbp but not hGHbp, or for mutants binding hGHbp but not hPRLbp, respectively.
4 5 Binding and elution of phage was carried out in each cycle as follows:
1. BINDING: M aliquot of hormone phage (typically 10S -1010 CFU) was mixed with an equal amount of non-hormone phage (pCAT), diluted into the appropriate buffer (A, B, or C), and mixed with a 10 mL suspension of hGHbp, hPRLbp or blank beads in a total volune of 200m1 in a t.5 ml pdypropylene tube. The phage were allowed b bind b the beads by incubating t hour at room temperature (23oC) with slow rotation (approximately 7 RPM). S~sequent steps were carried out with a constant volume of ZOO~L and at room temperature.
2. WASHES: The beads were spun t5 sec., and the supernatant was removed. To reduce the rx~mber of 5 phage not speafically bound, the beads were washed 5 times by resuspending briefly in the appropriate buffer, then pelleting.
3. hGH ELUTION: Phage binding waaldy to the beads were by ek~tion with hGH.
The beads were rotated with the appropriate buffer containing 400 r~.hGH for 15-17 hours. The supernatant was saved as the 'hGH elution' and the beads. The beads were washed by resuspending Ixiefly in buffer and pelleting.
Binding of hormone-phage to beads was carried out ~ one of the following buffers: Buffer A (PBS, 0.5% BSA, 0.05°~ Tween 20, 0.01°~ thimerosal) for selections using hGHbp and blank beads; Buffer B (50 mM
tris pH 7.5,10 mM MgCl2, 0.5% BSA, 0.05°~ Tween 20,100 mm ZnCl2) for selections using hPRLbp in the presence of zinc (+ Zn2+); or Buffer C (PBS, 0.5% BSA, 0.05% Tween 20, 0.01%
thimerosal, 10 m~ EDTA) for selections using hPRLbp in the absence of zinc (+ EDTA). Binding selections were carried out according to each of tt~e following paths: (1) binding to blank beads, (2) binding to hGHbp-beads, (3) binding to hPRLbp-beads (+
Zn2+), (4) binding to hPRLbp-beads (+ EDTA), (5) pre-adsorbing twice with hGHbp beads then binding the non-adsorbed fraction to hPRLbp-beads ('-hGHbp, +hPRLbp' selection), or (6) pre-adsorbing twice with hPRLbp-beads then binding the non-adsorbed fraction to hGHbp-beads ('-hPRLbp, +hGHbp' selection). The latter two procedures are expected to enrich for mutants binding hPRLbp but not hGHbp, or for mutants binding hGHbp but not hPRLbp, respectively.
4 5 Binding and elution of phage was carried out in each cycle as follows:
1. BINDING: M aliquot of hormone phage (typically 10S -1010 CFU) was mixed with an equal amount of non-hormone phage (pCAT), diluted into the appropriate buffer (A, B, or C), and mixed with a 10 mL suspension of hGHbp, hPRLbp or blank beads in a total volune of 200m1 in a t.5 ml pdypropylene tube. The phage were allowed b bind b the beads by incubating t hour at room temperature (23oC) with slow rotation (approximately 7 RPM). S~sequent steps were carried out with a constant volume of ZOO~L and at room temperature.
2. WASHES: The beads were spun t5 sec., and the supernatant was removed. To reduce the rx~mber of 5 phage not speafically bound, the beads were washed 5 times by resuspending briefly in the appropriate buffer, then pelleting.
3. hGH ELUTION: Phage binding waaldy to the beads were by ek~tion with hGH.
The beads were rotated with the appropriate buffer containing 400 r~.hGH for 15-17 hours. The supernatant was saved as the 'hGH elution' and the beads. The beads were washed by resuspending Ixiefly in buffer and pelleting.
10 4. GLYCINE ELUTION: To remove the tightest-binding phage (i.e. those still bound after the hGH
wash), beads were suspended in Glycine buffer (Buffer A plus 0.2 ~Glycine, pH
2.0 with HCI), rotated t hour and pelleted. The supernatant ('Glyane elution'; ZOOR.L) was neutralized by adding 30 ml of 1 M Tris base and stored at 4o C.
5. PROPAGATION: Aliquots from the hGH elutions and from the Glyane elutions from each set of 15 beads under each set of conditions were used to infect separate cultures of log-phase XLt-Blue cells.
Transductions were carried out by mixing phage with 1 mL XLt-Blue cells, incubating 20 min. at 37oC, then adding K07 (moi= t00). Cultures (25 mL 2YT plus carbenicillin) were grown as described above and the next pool of phage was prepared as described above.
Phage binding, elution, and propagation were carried out in successive rounds, according b the cycle 20 described above. For example, ~e phage amplified from the hGH elution from hGHbp-beads were again selected on hGHbp-beads and eluted with hGH, then used to infect a new culture of XL1-Blue cells. Three to five rounds of selection and propagatan were carried out for each of fhe selection procedures described above.
From the hGH and Glyane elution steps of each cycle, an aliquot of phage was used to inoculate XLt-Btue 25 cells, which were plated on LB media containing carbenialGn and tetracyGine to obtain independent doves from each phage pool. Single-stranded DNA was prepared from isolated cobny and sequenced in the region of the mutagenic cassette. The results of DNA sequencing are summarized in terms of the deduced amino acid sequerx;es in Figures 5, 6, 7, and 8.
3 0 ~.i~B~,h~H,~
To determine the tHnding affinity of some of the selected hGH mutants for the hGHbp, we transformed DNA from sequenced clones into ~ strain 1609. As described above, this is a non-suppressor strain which terminates translation of protein after the final Phe-191 residue of hGH.
Single-stranded ONA was used for these transformations, but double-stranded DNA or even wtrole phage can be easily electroporated into a non-35 suppressor strain for expression of free hormone.
Mutants of hGH were prepared from osmotically shocked cells by ammonium sulfate precipitation as described for hGH (Olson et al., ~g 293, 408-41 t (1981)), and protein concentrations were measured by laser densitomoetry of Coomassie-stained SDS-polyacrylamide gel electrophoresis gels, using hGH as standard (Cunningham and Wells, 244, 1081-1085 (1989)).
The binding affinity of each mutant was determined by displacement of 1251 hGH
as described (Spencer et al., J. Biol. Chem. 263, 7862-7867 [1988] ; Fuh et al., J. Biol. Chem. 265, 3111-3115 [1990]), using an anti-receptor monoclonal antibody (Mab263).
The results for a numt~er of hGH mutants, selected by different pathways (Fig.
6) are shown in Table VII. Many of these mutants have a tghter binding affinity for hGHbp than wild-type hGH. The most improved mutant, KSYR, has a binding affinity 5.6 times greater than that of wed-type hGH. The weakest selected mutant, among those assayed was only about 10-fold lower in binding affinity than hGH.
Binding assays may be carried out for mutants selected for hPRlbp-binding.
Table VII.
Cotre bhdhg b tlGiibP
The selected pool in which each mutant was found is indicated as 1 G (first glyclne selection), 3G (third glycine selection), 3H (third hGH selection), 3' (third selection, not tlinding to hPRI_bp, but Minding to hGHbp).
The number of times e~h mutant ocLKrred among all sequerxed Bones is shown ().
Mutant Kd (nM) Kd(mut)/Kd(hGH) Pod KSYR (6) 0.06 + 0.01 0.18 1G,3G
RSFR 0.10 + 0.05 0.30 3G
RAYR 0.13 + 0.04 0.37 3' KTYK (2) 0.16 + 0.04 0.47 H,3G
RSYR (3) 0.20 + 0.07 0.58 ~ 1G,3H,3G
KAYR (3) 0.22 + 0.03 0.66 3G
RFFR (2) 0.26 + 0.05 0.76 3H
KQYR 0.33 + 0.03 1.0 3G
KEFR= wt (9) 0.34 + 0.05 1.0 3H,3G,3' RTYH 0.68 + 0.17 2.0 3H
QRYR 0.83 + 0.14 2.5 3' KKYK 1.1 +0.4 3.2 3' RSFS (2) 1.1 + 0.2 3.3 3G,' KSNR 3.1 + 0.4 9.2 3' At some residues, substitution of a particular amino acrd has essentially the same effect independent of surrounding residues. For example. substitution of F176Y in the background of 172Rl174S redtxes tHnding affinity by 2.0-fold (RSFR vs. RSYR). Similarly, in the background of 172KI174A the tHnding affinity of the F176Y mutant (KAYR) is 2.9-fold weaker than the corresponding 176F mutant (KAFR; Cunningham and Wells, 1989).
WO 92/09690 ~ ~ ~ ~ ~ ~ ~ PCT/US91 /09133 On the other hand, the binding constants determined for several selected mutants of hGH demonstrate ran~additive etfeds of some amino aad substitutions at residues 172,174,176, and 178. For example, in the background of 172KI176Y, the substitution E174S results in a mutant (KSYR) which binds hGHbp 3.7-fold tighter than the corresponding mutant containing E174A (KAYR). However, in the background of 172R/176Y, the effects of these E174 substitutions are reversed. Here, the E174A mutant (RAYR) binds 1.5-fold tighter than the E174S mutant (RSYR).
Such non~additive effects on binding for substitutions at proximal residues illustrate the utility of protein-phage binding selek~ion as a means of selecting optimized mutants from a library m~ndomized at several positions. ~ the absence of deta~ed swdural information, without such a selection process, many combinations of substitutions might be tried before finding the optimum mutant.
EXAMPLE IX
SELECTION OF hGH VARIANTS FROM A HELIX~1 RANDOM CASSETTE
LIBRARY OF HORMONE-PHAGE
Using the methods described in Example VIII, we targeted another region of hGH
involved in binding to the hGHbp andlor hPRLbp, helix 1 residues 10,14,18, 21, for random mutagenesis in the phGHam~3p vector (also known as pS0643; see Example VIII).
We chose to use the'amber' hGH-g3 construct (called phGHam~g3p) because it appears to make the target protein, hGH, more accessible fx binding. This is supported by data from comparative ELISA assays of monodonaJ antibody binding. Phage produced from both pS0132 (S. Bass, R.
Greene, J. A. Wells, Proteins 8, 309 (1990).) and phGHam-g3 were tested with three antibodies (Medix 2,1 B5.G2, and 5B7.C10) that are known to have binding detertninar>is near the carboxyl-terminus of hGH [B. C.
Cunningham, P. Jhurare, P. Ng, J. A. Wells, Saenee 243,1330 (1989); B. C. Cunningham and J. A. Wells, Silence 244,1081 (1989); L. Jin and J. Wells, unpublished results], and one antibody (Medix 1 ) that recognizes determinants in helices 1 and 3 ([B. C.
Cunningham, P. Jhurani, P. Ng, J. A. Wells, Saerx;e 243,1330 (1989); B. C.
Cunringham and J. A. Wells, Science 244,1081 (1989)]). Pt~agemid partides from phGHam-g3 reacted much more strongly with antibodies Medix 2, 185.62, and 5B7.C10 than did phagemid partiGes from pS0132. In particular, binding of pS0132 particles was reduced by >2000-fold for both Medix 2 and 5B7.C10 and reduced by >25-fold for 1B5.G2 compared to binding to Medix 1. On the other hand, binding of phGHam~3 phage was weaker by only about 1.5-fold,1.2-fold, and 2.3-fold for the Medix 2,1 B5.G2, and 587.C10 antibodies, respectively, compared with binding to MEDIX 1.
We mutated residues in helix 1 that were previously identified by alar>ine-scanning mutager~esis (B. C.
Cunr>rngham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989); B. C.
Cunr>ingham and J. A. Wells, Science 244, 1081 (1989),15, 16) to modulate the binding of the extracellular domains of the hGH andlor hPRL
receptors (called hGHbp and hPRLbp, respectively). Cassette mutagenesis was varied out essentially as described [J. A. Wells, M. Vasser, D. B. Powers, Gene 34, 315 (1985)]. This library was constructed by cassette mutagenesis that fully mutated four residues at a time (see Example VIII) which utilized a mutated version of phGHam-g3 into which unique Kpnl (at hGH codon 27jand Xhol (at hGH codon 6) restriction sites (underlined below) had been inserted by mutagenesis [ T. A Kunkel, J. D. Roberts, R. A
Zakour, Methods EnzymoL 154, 367-2~9~~~3 38 382] with the oligonucleotides 5'-GCC TTT GAC AGG TAC CAG GAG TTT G-3' and 5'-CCA ACT ATA CCA
CTS TCG AGG TCT ATT CGA TAA C-3', respectively. The later oligo also introduced a +1 frameshift (italidzed) to terminate translation from the starting vector and minimize wild-type background in the phagemid library. This strafing vector was designated pH0508B. The helix 1 library, which mutated hGH residues 10, 14, 18, 21, was constructed by ligating to the large Xhol-Kpnl fragment of pH0508B
a cassette made from the complementary oiigonucleotides 5'-pTCG AGG CTC NNS GAC AAC GCG NNS CTG CGT GCT
NNS CGT CTT
NNS CAG CTG GCC TTT GAC ACG TAC-3' and 5'-pGT GTC AAA GGC CAG CTG SNN AAG ACG
SNN AGC
ACG CAG SNN CGC GTT GTC SNN GAG CC-3'. The Kpnl site was destroyed in the junction of the ligation product so that restriction enzyme digestion could be used for analysis of non-mutated background.
The library contained at least 10~ indeperkient transfonnaMs so that if the library were absolutely random ( 106 different combinations of colons) we would have an average of about 10 copies of each posside mutated hGH gene. Restriction analysis using Kpni indicated that at least 80%
of helix 1 library constructs contained the inserted cassette.
Binding enrichments of hGH-phage from the libraries was carried out using hGHbp immobilized on oxirane-polyacrylamide beads (Sigma Chemical Co.) as described (Example VIII).
Four residues in helix 1 (F10, M14, H18, and H21 ) were similarly mutated and after 4 and 6 cycles a non-wild-type consensus developed (Table VIII). Position 10 on the hydrophobic face of helix 1 tended to be hydrophobic whereas positions 21 and 18 on the hydrophillic face tended were dominated by Asn; no obvaus consensus was evident for position 14 (Table IX).
The binding constants for these mutants of hGH to hGHbp was determined by expressing the free hormone variants in the non-suppressor E. coli strain 1609, purifying the protein, and assaying by competitive displacement of labelled wt-hGH irom hGHbp (see F~cample VIII). As indicated, several mutants bind tighter to hGHbp than does wt-hGH.
Table VIII.
Selection of hGli helix 1 mutants Variarns of hGH (randomly mutated at residues F10, Mt4, H18, H21) expressed on phagemid particles were selected by binding to hGHbp-beads and eluting with hGH (0.4 mIN) buffer followed by gfycine (0.2 M, pH 2) buffer (see Example VIII).
Gly elution 4 Cycles H G N N
A W D N (2) Y T V N
I N I N
L N S H
F S F G
6 Cycles H G N N (6) F S F L
Consensus:
H G N N
~~95~3~ 40 Table IX
Ca~enst8 sequels tram the selected helix 1 Nbrary Observed frequency is tn~tion of all doves sequerxed with the indicated amino aad. The nominal frequency is cabulated on the basis of NNS 32 colon degeneracy. The maximal enrichment facto varies from 11 ~ 32 depending upon the rwminal frequency value for a given residue. Values of [Kd(Ala mut)IK d(wt hGH)J for single alanine mutations ware taken from B. C. Cumingham and J. A. Wells, Sderx~e 244,1081 (1989); B. C. Cunningham, D. J. Henner, J. A. Wells, Soaves 247,1461 (1990); B. C. Cunningham and J. A.
Wells, Proc. Nail. Acad Sa. USA
88, 3407 (1991 ).
wld type Selected Kd(Ala mut) residue Kd(wt hGH) re~due observed rornir~al Enrichment F10 5.9 H 0.50 0.031 17 F 0.14 0.031 5 A 0.14 0.062 2 M14 2.2 G 0.50 0.062 8 W 0.14 0.031 5 N 0.14 0.031 5 S 0.14 0.093 2 H18 1.6 N 0.50 0.031 17 D 0.14 0.031 5 F 0.14 0.031 5 H21 0.33 N 0.79 0.031 26 H 0.07 0.031 2 Table X
t8lndnp d puri~d hGH helix 1 rt~nts b hGHbp Competition Minding experiments were performed using [l~IjhGH (wild-type), hGHbp (containing the extracellular receptor domain, residues 1-238), and Mab263 [B. C. Cumingham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989));. The number P indicates the fractional ocaxrence of each mutant among all the clones sequenced after one or more rounds of selection.
Sequence P Kd (nA~\f(Kd Kd(wt pogtion mut) hGH)) H G N N 0.50 0.14 t 0.04 0.42 A W D N 0.14 0.100.03 0.30 wt= F M H H 0 0.34 0.05 (1 ) F S F L 0.07 0.680.19 2.0 Y T V N 0.07 0.750.19 2.2 L N S H 0.07 0.82 t 0.20 2.4 I N I N 0.07 12 ~ 0.31 3.4 EXAMPLE X
SELECTION OF hGH VARIANTS FROM A HEUX-4 RAN00M CASSETTE LIBRARY CONTAINING
Our experience with reauiting non-binding homologs of hGH evolutionary variants suggests that many individual amino acid substitutions can be combined to yield cumulatively improved mutants of hGH with respect to binding a particular receptor (B. C. Cunnirgham, D. J. Henner, J. A. Wells, Scien~oe 247,1461 (1990); B. C.
Cunningham and J. A. Wells, Pros. Nad. Acad Sa. USA 88, 3407 (1991 ); H. 8.
Lowman, B. C. Cumingham, J. A.
3 5 Wells, J. 8iol. Chem. 266, in press ( 1991 )j.
The helix 4b library was constructed in an attempt to further improve the helix 4 double mutant (E174SIF176Y) selected from the helix 4a library that we found bound tighter to the hGH receptor (see Example VIII). With the E174S/F176Y hGH mutant as the background starting hormone, residues were mutated that surrounded positions 174 and 176 on the hydrophiNc face of helix 4 (R167, D171, T175 and 1179) .
Cassette mutagenesis was carried out essentially as described [J. A. Wells. M.
Vasser, D. B. Powers, Gene 34, 315 (1985)). The helix 4b library, which mutated residues 167,171,175 and 179 within the E174S/F176Y background, was constructed using cassette mutagenesis that fully mutated four residues at a time (see Example VIII) and which utilized a mutated version of phGHam~3 into which uryque BstBl and BgAI
restriction sites had been inserted prevausly (Example VIII). into the BstEll-Bglll sites of the vector was inserted a cassette made from the complementary oligonudeotides 5'-pG TTA CTC TAC TGC
TTC NNS AAG GAC ATG
NNS AAG GTC AGC NNS TAC CTG CGC NNS GTG CAG TGC A-3' and 5'-pGA TCT GCA CTG
CAC SNN
GCG CAG GTA SNN GCT GAC CTT SNN CAT GTC CTT SNN GAA GCA GTA GA-3'. The BstEfl site was eliminated in the ligated cassette. From the helix 4b litxary,15 unselectsd doves were sequenced. Of these, none lacked a cassette insert, 20% were frame-shifted, and 7% had a non-silent mutatan.
Binding enrichments of hGH-phage from the libraries was carried out using hGHbp immobilized on oxirane-polyaaylamide beads (Sigma Chemical Co.) as described (Example VIII).
After 6 cycles of binding a reasonably dear consensus developed (Table XI). Interestingly, all positions tended to contain polar residues, notably Ser, Thr and Asn (XII).
The binding constants for some of these mutants of hGH to hGHbp was determined by expressing the free hormone variants in the non-suppresser E. cell strain 16C9, purifying the protein, and assaying by competitive displacement of labelled wtfiGH from hGHbp (see Example VIII). As indicated, the binding affinities of several helix-4b mutants for hGHbp were tighter than that of wt-hGH Table XIII).
Finally, we have begun to analyze the binding affinity of several of the tghter hGHbp binding mutants for their ability to bind to the hPRLbp. The E174S/F176Y mutant binds 200-fold weaker to the hPRLbp than hGH. The E174T/F176YIR178K and R167N/D171SIE174S/FI76YII179T mutants each bind >500-fold weaker to the hPRLbp than hGH. Thus, it is possible be use the produce new receptor selective mutants of hGH by phage display tectx~ology.
Of the 12 residues mutated in three hGH-phagemid libraries (Examples VIII, lX, X), 4 showed a strong, although not exclusive, conservation of the wild-type residues (K172, T175, F176, and R178). Not surprisingly, these were residues that when converted to Ata caused the largest disruptions (4- to 60-fold) in binding affinity to the hGHbp. There was a class of 4 other residues (F10, M14, D171, and 1179) where Ala substitutions caused weaker effects on binding (2- to 7-fold) and these positions exhibited little wild-type consensus. Finally the other 4 residues (H18, H21, 8167, and E174), that promote binding to he hPRLbp but not the hGHbp, did not exhibit any consensus for the wild-type hGH sequence by selection on hGHbp-beads. In fact iwo residues (E174 and H21 ), where Ala substitutions enhance binding affinity to the hGHbp by 2- to 4-fold [B. C. Cunningham, P.
Jhurani, P. Ng, J. A. Wells, Sdenoe 243,1330 ( 1989); B. C. Cunningham and J.
A Wells, Scenes 244,1081 (1989); B. C. Cunr>ingham, D. J. Henner, J. A. Wells, Saerx;e 247,1461 (1990);
B. C. Cunningham and J. A, Wells, Proc. Natl. Acad. Sa. USA 88, 3407 (1991 )]. Thus, the alanine-scanning mutagenesis data correlates reasonably well with the flexibility to substitute each position. In fact , the reduction in binding affinity caused by alanine substitutions [B. C. Cunningham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989); B. C. Cunningham and J. A. Wells, Sdenoe244,1081 (1989)], B. C. Cunningham, D. J. Henner, J. A.
Wells, Science247, 1461 (1990); 8.
C. Cunningham and J. A. Wells, Pros. Natf. Acad. Sa. USA 88, 3407 ( 1991 )] is a reasonable predictor of the percentage that the wild-type residue is found in the phagemid pool after 3-6 rounds of selection. The alanine-WO 92/09690 ~ ~ ~ ~ PCT/US91/09133 scanning information is useful for targeting side-chains that modulate binding, and the phage selection is appropriate for optimizing them and defining the flexibility of each site (and/or combinations of sites) for substitution. The comt~ir~tion of scanr~rg mutational methods [B. C.
Cunningham, P. Jhurani, P. Ng, J. A. Wells, Science ?A3,1330 (1989); B. C. Cunningham and J. A Wells, Saenc~e ?A4,1081 ( 1989)] and phage display is a powerful approach to designing reoepta-ligand interfaces and sdrdying molecular evolution in vitro.
In cases where combined mutatans in hGH have additive effects on binding affinity bo receptor, mutations learned through hormone-phagemid enrichment to improve bindirg can be combined by simple cutting and ligation of resfiction fragments or mutagenesis to yield cumulatively optimized mutants of hGH.
On the other hand, mutations in one region of hGH which optimize receptor binding may be stn~cturally or functionally compatible witty mutations in an overlapping a another region of the molecule. In these cases, hormone phagemid enrichment can be cartied out by one of several variatans on the iterdtiHe emid~ment approach (t ) random DNA litxaries can be generated in each of two (or perhaps more) regions of the molecule by cassette or another mutagenesis method. Thereafter, a combined library can be created by ligation of restriction fragments from the two ONA libraries; (2) an hGH variant, optimized for binding by mutation in one region of the molecule, can be randomly mutated in a second region of the molecule as in the helix-4b library example; (3) two or more random libraries can be y selected for improved binding by hormone-phagemid enrichment; after this 'roughing-in' of the optimized binding site, the still-partially-diverse libraries can be recombined by ligation of restriction fragments Go generate a single library, partially diverse in iwo or more regions of the molecules, which in tum can be further selected for optimized binding using hormone-phagemid enrichment.
2Q9~~'a~
Table XI.
AAumnt pt~erNds d hGH sHecbed from helix 4b library after 4 and 6 cyGes of enrichment Selection of hGH helix 4b mutants (randomly mutated at residues 167, 171,175,179), each containing the E174SIF176Y
double mutant, by binding to hGHbp-beads and eluting with hGH (0.4 mA~ buffer followed by glyane (0.2 M, pH 2) buffer.
One mutant (+) contained the spurious mutation R178H.
8167 Di71 T175 1179 4 Cydes N S T T
K S T T
S N T T
D S T T
D S T T+
D S A T
D S A N
T D T T
N D T N
A N T N
A S T T
6 CyGes N S T T (2) N N T T
D S S T
E S T I
K S T L
Cor~r~s:
N S T T
D N
WO 92/09690 ~ ~ j1 ;~ ~ ~ ~ PGT/US91/09133 Tale )01 Cornell~s ~puerroes from the >teleaed library.
Observed frequency is traction of aA cbnes sequenced with the indicated amino aad. The nominal frequerxy is calculated on the basis of NNS 32 colon degeneracy. The maximal enrichment factor varies from 11 to 16 to 32 5 depending upon the raminal frequency value for a given residue. Values of [Kd(Ala mut)IKd(wt hGH)] for single alanine mutations were taken from refs. below; for position 175 we oNy have a value br the T175S mutant [B. C.
Cunningham, P. Jtxuani, P. Ng, J. A. Wells, Saenoe 243,1330 (1989); B. C.
Cumingham and J. A. Wells, Saence 244, 1081 (1989); B. C. Cunrungham, D. J. Henner, J. A. Wells, Saenoe 247,1461 (1990); B. C. Cunningfam and J. A Wells, Proc. Nail. Acad Sci. USA88, 3407 (1991).).
Wild type Selected residue Kd(~a mut) residue observed raminal Enrichment Kd(wt hGH) 8167 0.75 N 0.35 0.031 11 D 0.24 0.031 8 K 0.12 0.031 4 A 0.12 0.062 2 D171 7.1 S 0.76 0.093 8 N 0.18 0.031 6 2 0 D 0.12 0.031 ' 4 T175 3.5 T 0.88 0.062 14 A 0.12 0.031 4 1179 2.7 T 0.71 0.062 11 N 0.18 0.031 6 Table XIII
t3Ndirrg of purified trGH mutar>m to hGHbp.
Competition binding experiments were performed using [1251]hGH (wild-type), hGHbp (containing the extraceNular receptor domain, residues 1-238), and Mab263 (11). The number P
indicates the fractional oaxxrence of each mutant among aU the doves sequenced after one or more rods of selection. Note that the helix 4b mutations (") are in the background of hGH(E174SIF176Y). In the list of helix 4b mutants" the E174SIF176Y mutant ('), with wt residues at 167,171,175, 179, is shown in bold.
Kd(Ala m~) SequerxePosition P Kd(n d(w~ t hGH) N S T T 0.18 0.04 t 0.02 0.12 E S T I 0.06 0.04 0.02 0.12 K S T L 0.06 0.05 0.03 0.16 N N T T 0.06 0.06 0.03 0.17 R D T I 0 0.06 0.01 (0.18) N S T Q 0.06 0.26 0.11 0.77 WO 92/09690 r' PCT/US91/09133 2~~~~~3~~
Assembly d F~ ~Aolea~le on tie Pld Sur<aoe Ptasmid pDH 188 captains the DNA encoding the F~ portion of a humanized IgG
antibody, called 4D5, that recognizes the HER-2 receptor. This plasmid is contained in E. coN strain SR 101, and has been deposited with the ATCC in RodcviNe, MD.
Briefly, the plasmid was prepared as follows: the starting plasmid was pS0132, oonta~ing the alkaline phosphatase promobar as described above. The DNA encoding human growth hormone was exased and, after a series of manipulations to make the ends of the plasmid compatible for ligatbn, the DNA encoding 4D5 was inserted. The 4D5 DNA contains two genes. The first gene encodes the variable and constant regions of the light chap, and contains at its 5' end the DNA erxxxJing the st II signal sequence.
The second gene cor>tains four portions: first, at its 5' end is the DNA erxodMg the st II signal sequence.
This is followed by the DNA erxoding the variable domain of the heavy chain, which is folbwed by the DNA encoding the first domain of the heavy chain constant region, which in turn is iolbwed by the DNA encoding the M13 gene III. The salient features of this construct are shown in Fgure 10. The sequence of the DNA erxxding 4D5 is shown in Fgure 11.
Both polyethylene glycol (PEG) and electroporation were used to transform plasmids into SRlOt cells.
(PEG competent cells were prepared and transformed according to the method of Chung and Miller (Nucleic Acids Res.16:3580 [1988]). Cells that were competent for electroporatbn were prepared, and subsequently transformed via electroporation according to the method of Zabarovsky and Winberg (Nucleic Acids Res.18:5912 [1990]). After placng the cells in 1 ml of the SOC media (described in Sambrook et al., supra), they were grown for 1 hour at 37°C with shaking. At this time, the concentration of the cells was determined using light scattering at ODSpO. A titered K07 phage stock was added bo achieve an multiplicity of infection (M01) of 100, and the phage were albwed to adhere to the cells for 20 minutes at room temperature.
This mixture was then diluted into 25 mls of 2n broth (described in Sambrook et al., supra) and incubated with shaking at 37°C overnight. The next day, cells were pelleted by centrifugation at 5000 x g for 10 minutes, the supernatant was collected, and the phage partices were precipitated with 0.5 M NaCI and 4% PEG (final corxentration) at room temperature for 10 minutes. Phage partices were pelleted by centrifugatbn at 10,000 x g for 10 minutes, resuspended in 1 ml of TEN
(10 mM Tris, pH 7.6,1 mM EDTA, and 150 mM NaCI), and stored at 4°C.
Aliquots of 0.5 ml from a solution of 0.1 mg/ml of the extra~etlutar domain of the HER-2 antigen (ECD) or a solution of 0.5 mg/ml of BSA (control antigen) in 0.1 M sodium bicarbonate, pH 8.5 were used to coat one well of a Falcon 12 well tissue culture plate. Once the solution was applied to the wells, the plates were incubated at 4°C on a rocking plattortn overnight. The plates were then blocked by removing the initial solution, applying 0.5 ml of blocking buffer (30 mg/ml BSA in 0.1 M sodium bicarbonate), and incubating at room temperature for one hour.
Finally, the blocking buffer was removed, 1 ml of buffer A (PBS, 0.5% BSA, and 0.05% Tween-20) was added, and the plates were stored up to 10 days at 4°C before being used for phage selection.
a~
Approximately 109 phage particles were mixed with a 100-fold excess of K07 helper pfrage and 1 ml of buffer A . This mixhxe was divided irno two 05 ml aliquots; one of which was applied to ECD coated wells, and the other was applied b BSA coated weus. The plates were ina~batad at room temperature while shaking for one to ttxee hours, and were then washed three times over a period of 30 mirurtes wish 1 ml aliquots of buffer A.
Elution of the phage from the plates was done at room temperature by one of two methods: t ) an initial overnight incubation of 0.025 mglml purified Mu4D5 antibody (murine) folbwed by a 30 minute irxubation with 0.4 ml of the add elution buffer (0.2 M glydne, pH 2.1, 0.5% BSA, and 0.05% Tween-20), or 2) an incubation with the acid elution tx~ffer alone. Eluates were then neutralized with 1 M Tris vase, and a 0.5 ml aliquot of TEN was added.
These samples were then propagated, titered, and shred at 4°C.
Alquots of eluted phage were added to 0.4 ml of 2YT broth and mixed with approximately 108 mid-log phase male E. cbli strain SR101. Phage were allowed b adhere to the cells for 20 minutes at room temperature and then added to 5 ml of 2YT broth that contained 50 R.giml of carbenidllin and 5 ~g/ml of tetracycline. These cells were grown at 37°C for 4 to 8 hours until they reached mid-log phase. The OD6pp was determined, and the cells were superinfected with K07 helper phage for phage production. Orxe phage particles were obtained, they were titered in order to determine the number of colony forming urNts (cfu).
This was done by taking aliquots of serial dilutions of a given phage stock, allowing Ihem to infect mid-log phase SRt 01, and plating on LB plates oontaimg 50 uglml c~rbeniciuin.
2 0 ~ p8fllZd, The affinity of h4D5 Fab fragments and Fab phage for the ECD antigen was determined using a competitive receptor Minding RIA (Burt, D. R., Receptor Bending in Dnrg Research. 0'Brien, R.A. (Ed.). pp. 3-29, Dekker, New York [1986)). The ECD antigen was labeled with 125-Ipd;ne using the sequential c~loramine-T
method (De Larco, J. E. et al., J. Ceff. PhysioJ.109:143-152 [1981]) which produced a radioactive tracer with a speafic activity of l4~Cilf,ig and incorporation of 0.47 moles of Iodine per mole of receptor. A series of 0.2 ml solutions containing 0.5 ng (by ELISA) of F~ or F~ phage, 50 nCi of 1251 ECD
tracer, and a range of unlabeled ECD amounts (6.4 ng to 3277ng) were prepared and incx~bated at room temperature overnight. The labeled ECD-F~ or ECD-FaM phage complex was separated from the unbound labeled antigen by forming an aggregate complex induced by the addition of an anti-human IgG (Fitzgerald 40-GH23) and 6°~ PEG 8000. The complex was pelleted by centrifugation (15,000 x g for 20 minutes) and the amount of labeled ECD (in cpm) was determined by a gamma counter. The dissoaation constant (Kd) was cala~lated by employirg a modified version of the program LIGAND (Munson, P. and Rothbard, D., Anal. &ochem.107~20-239 [1980)) which utilizes Scatchard analysis (Scatchard, G.,Ann. N.Y. Acad. Sci. 51:66072 (1949]). The Kd values are shown in Figure 13.
Murine 4D5 antibody was labeled with 125-l to a spedfic activity of 40-50 ~Cilpg using the lodogen procedure. Solutions containing a constant amount of labeled antibody and increasing amounts of unlabeled variant Fab were prepared and added to near confluent cultures of SK-BR-3 cells grown in 96-well microtiter dishes (final concentration of labeled antibody was 0.1 nM). After an overnight incubation at 4°C, the supernatant was removed, the cells were washed and the cell assodated radioactivity was determined in a gamma counter. Kd wakres were determined by analyzing ltte data usirta a motifi9d version of the program t.IGAND (MunsorL P. end Rothbaro, D., supra?
This depOSit of pldsmid pDHt 89 ATCC no. 68683 was made und9r the provisions of tkte St~dapest Treaty or1 the International Recognition of the Deposit of Micraorgsnisms for the Purpose of 5 patent Procedure and the Regulations thereunder (Budapest Treaty). This assures maintenance of a viable Culture for 30 ye8rs from the date of deposit. The organisms will be made available by ATCC
under the terms of the Budapest Treaty, and subject t0 an agreement between Genentech, Inc. End AT~~, which assures permanent and unrestricted availability of the progeny of the cultures to the public upon issuance of a f $tent On the basis of the application, or the patent appllGation is refused, or i5 abandoned and no longer subject to reinstatement, or is withdrawn, whichever oomes first, and assures Q availability of the progeny t0 One determined by the Commissioner of Pntetlt5 to be entitled thereto according to Section 109 of the Patent l=lutes.
The assignee of the present application has agreed that If the cultures on deposit sttoutd d'le or be Iosi or destroyed when cultivated under suitable t>onditiats, they wiN be promptly replaced on rtotificatan wi>rt a viable 15 specimen of the same a.~ture. Availability of the deG4sibed cultures is rtot to be construed as a license to practice the invention in contravention of the rights granted under tile authority of any government in accordance with its patent laws.
The fxegdrtg written spedt'~abwt is considered to be suttident to enable one skilled in the art to practice the invention. The present ~ven>ion is not to be limited in scope by ttte cultures deposited, since the 20 deposited embodiments are Intended as separate illustrations of certain aspects at the invention and any a~tures that are ftatctionally equivalent are within fhe sa~pe of this irnrention. The deposit of material herein does not constitute an admission that the wriaen description hlereln contained is inadequate to enable the pracBce of any aspect of the invention, including the best mode thereof, nor is it to be CansUtAad as limiting the soop2 of tt"to dairns to the specific illustrations that it represents. Indeed, Parlous modifications of the irnention in addition to 25 those shown 2ind descrit~ed herein wiU become apparent to those skilled in tile an from the foregoing desaipCioh and fall within me scope of the appended Balms.
whaa ttte irrrer~tion has neoessaray been desaitied in coftjurtction wilt prelerted embodiments, one of ordinary skin, after reading the toregaing speafication, w~l be able to etrect various changes, substitutions of equivalents, and alterations de Ute sut~ed rnatter set forth herein, widtout departing lrom the spirit and scone 34 thereof. Hence, the invention can be practiced in ways outer than those speafica~y desaibed herein. ft is therefore intended that the protetdon granted by ~et4ars Patent hereon be mired artly by the appended claims and equivalents thereof.
WO 92/09690 '~ ~ ~ f; ~' j ~ PCT/US91 /09133 EXAMPLE Xll SELECTION OF hGH VAi'~ANTS FROM COMBINATIONS OF HELIX-1 AND HEUX-4 HORMONE-PHAGE
VARIANTS
According to additivity principles ~J. A. Wells, Biochemistry29, 8509 (1990)j, mutations in different parts of a protein, if they are not mutually interacting, are expected to combine to produce additive changes in the free energy of Minding to another molecule (changes are additive in terms of AOGb;~ing, or multiplicative in terms of Kd = exp[-eGIRTj ). Thus a mutation produang a 2-fold increase in binding affinity, when comtHned with a second mutation causing a 3-fold increase, would be predicted to yield a double mutant with a 6-fold increased affinity over the starting variant.
To test whether multiple mutations obtained firom hGH-phage selections would produce cumulatively favorable effects on hGHbp (hGH-binding protein; the extracellular domain of the hGH receptor) Minding, we combined mutations found in the three tightest-binding variants of hGH from the helix-1 library (Example IX:
F10AIM14WIH18DlH21N, F10H/M14G/H18N/H21N, and F10FIM14S/H18F/H21L) with those found in the three tightest binding variants found in the helix-4b library (Example X:
R167N/D171SlT175II179T, R167EID171S/T175/I179, and R167N/D171NIT17511179T).
hGH-phagemid double-stranded DNA (dsDNA) from each of the one-helix variants was isolated and digested with the restriction enzymes EcoRl and BstXl. The large fragment from each helix-4b variant was then isolated and ligated with the small fragment from each helix-1 variant to yield the r~ew two-helix variants shown in Table XIII. All of these variants also contained the mutations E174S/F176Y
obtained in earlier hGH-phage binding selections (see Example X for details).
Although additivity prindples appear to hold for a number of comt~ir~ations of mutations, some comt~inations (e.g. E174S with F176Y) are Dearly non-additive (see examples VIII and X). In order to identify with certainty the tightest binding variant with, for example, 4 mutations in helix-1 ~ 4 mutations in helix-4, one would ideally mutate all 8 residues at once and then sort the pool for the globally tightest binding variant.
However, such a pool would consist of 1.1 x 1012 DNA sequences (utilizing NNS
colon degeneracy) encoding 2.6 x 1010 different polypeptides. Obtaining a random phagemid library large enough to assure representation of all variants (pefiaps 1013 transfonnants) is not practical using current transformation technology.
We have addressed this difficulty first by utilizing successive rounds of mutagenesis, taking the tightest Minding variant from one library, then mutating other residues to further improve binding (Example X).
In a second method, we have utilized the principle of additivity to comt~ir~e the best mutations from two independently sorted libraries to create multiple mutants with improved binding (described above). Here, we further searched through the possible comtHnations of mutations at positions 10,14,18, 21,167,171,175, and 179 in hGH, by creating comt~inatorial libraries of random or partially-random mutants. We constructed three different comt~inatorial libraries of hGH-phagemids, using the pooled phagemids from the helix 1 library (independently sorted for 0, 2, or 4 cycles; Example IX) and the pool from the helix-4b library (independently sorted for 0, 2, or 4 cyGes; Example X) and sorted the combined variant pool for hGHbp binding. Since some amount of sequence diversity exists in each of these pools, the resulting combinatorial library can explore more sequence combinations than what we might constmct manually (e.g. Table XIII).
WO 92/09690 2 ~ ~ ~ ~ ~ ~ PCT/US91 /09133 hGH-phagemid double-stranded DNA (dsDNA) from each of the one-helix library pools (selected for 0, 2, or 4 rounds) was isolated and digested with the restriction enzymes Ac~cl and BstXl. The large fragment from each helix-1 variant pool was then isolated and ligated with the small fragment from each helix-4b vaunt pool to yield the three combinatorial libraries pH0707A (unselecbed helix 1 and helix 4b pools, as described in examples IX
5 and X), pH0707B (twice-selected helix-1 pool with twice-selected helix-4b pool), and pH0707C (4-times selected helix-1 pool with 4-times selected helix-4b pool). Duplicate ligations were also set up with less DNA and designated as pH0707D, pH0707E, and pH0707F, corresponding to the 0-,2-, and 4-round starting libraries respectively. All of these variant pools also contained the mutations E174SIF176Y obtained in earlier hGH-phage binding selectans (see Example X for details).
The ligation products pH0707A-F were processed and electro-transformed into XL1-Blue cells as described (Example VIII). Based on colony-forming units (CFU), the number of transformants obtained from each pool was as follows: 2.4x106 from pH0707A, 1.8x106 from pH0707B, 1.6x106 from pH0707C, 8x105 from pH0707D, 3x105 from pH0707E, and 4x105 from pH0707F. hGH-phagemid particles were prepared and selected for hGHbp-binding over 2 to 7 cycles as described in Example VIII.
In additan to sorting phagemid libraries for tight-binding protein variants, as measured by equilibrium binding affinity, it is of interest to sort for variants which are altered in either the on-rate (kon) or the off-rate (koff) of binding to a receptor or other molecule. From thermodynamics, these rates are related to the equilibrium dissodation constant, I(d = (koff/kon). We envision that certain variants of a particular protein have similar Kd's for binding while having very different kon's and ko ff's.
Conversely, d~anges in Kd trom one variant to another may be due to effects on kon, effects on ko ff, or both. The pharmacological properties of a protein may be dependent on Minding affinity or on kon or koff, depending on the detailed mechanism of action. Here, we sought to identify hGH variants with higher on-rates to investigate the effects of d~anges in kon. We envision that the selection could alternatively be weighted toward koff by increasing the binding time and increasing the wash time andlor concentration with cognate ligand (hGH).
From time-course analysis of wild-type hGH-phagemid Minding to immobilized hGHbp, it appears that, of tt~e total hGH-phagemid particles that can be eluted in the final pH 2 wash (see Example VIII for the complete Minding and elution protocol), less than 10% are bound affer 1 minute of incubation, while greater than 90% are bound affer 15 minutes of incubation.
For 'rapid-binding selection,' phagemid particles from the pH0707B pool (twice-selected for helices 1 and 4 independently) were incubated with immobilized hGHbp for only 1 minute, then washed six times with 1 mL of tHnding buffer; the hGH-wash step was omitted; and the remaining hGH-phagemid particles were eluted with a pH2 (0.2M glydne in binding buffer) wash. Enrichment of hGH-phagemid particles over non~iisplaying particles indicated that even with a short binding period and no cognate-ligand (hGH) d~allenge, hGH-phagemid binding selection sorts tight-Minding variants out of a randomized pool.
5, ~~ ~~3~
The binding constants for some of these mutants of hGH to hGHbp was determined by expressing the tree hormone variants in the non-suppressor E. colt strain 1609 or 3488, purifying the protein, and assaying by competitive displacement of labelled wt-hGH from hGHbp (see Example VIII) in a radio-immunoprecipitation assay.
In Table XIII -A below, all the variants have glutamate174 replaced by serine174 and pher~lalaninel7g replaced by tyrosine176 (E174S and F1176Y) plus the additional substitutions as indicated at hGH amino aad positions 10, 14,18, 21,167,171,175 and 179.
Table XIII-A
hGH
variaMS
from addpfon of helix-1 and helix~b mutaUons li H
li wild-type residue:F1Q e ~il,$ Jj21HlCtZ e I1Z~ 1128 ri x x nt X14 ~1Z1 Va H G N N N S T T
a In Table XIV below, hGH variants were selected from combinatorial libraries by the phagemid binding selection process. All hGH variants in Table XIV contain two background mutatans (E174SlF176Y). hGH-phagemid pools from the libraries pH0707A (Part A), pH0707B and pH0707E (Part B), or pH0707C (Part C) were sorted for 2 to 7 cycles for Minding to hGHbp. The number P indicates the fractional occurrence of each variant type among the set of Bones sequerxed from each pool.
WO PC'1'/US91/09133 ~~~~~~3 52 Table XIV
hGH variants from hOrllnor~e-pt~agemld binc9np selection Of comhlnalortal I~r~aries.
Hela Helix wild-type ~Q ~ J~$ ~ g~j residue:
Variant Part 4 cycles:
A :
0.60 H0714A.1 H G N N N S T N
0.40 H0714A.4 A N D A N N T N
' Part 8:
2 Cycles:
0.13 H0712B.1 F S F G H S T T
0.13 H0712B.2 H Q T S A D N S
0.13 H0712B.4 H G N N N A T T
0.13 H0712B.5 F S F L S D T T
0.13 H0712B.6 A S T N R D T I
0.13 H0712B.7 Q Y N N H S T T
0.13 H0712B.8 W G S S R D T I
0.13 H0712E.1 F L S S K N T V
0.13 H0712E.2 W N N S H S T T
0.13 H0712E.3 A N A S N S T T
0.13 H0712E.4 P S D N R D T I
0.13 H07t2E.5 H G N N N N T S
0.13 H0712E.6 F S T G R D T I
0.13 H0712E.7 M T S N Q S T T
0.13 H0712E.8 F S F L T S T S
4 cycles:
0.17 H0714B.1 A W D N R D T I
0.17 H0714B.2 A W D N H S T N
0.17 H0714B.3 M Q M N N S T T
0.17 H0714B.4 H Y D H R D T T
0.17 H0714B.5 L N S H R D T I
0.17 H0714B.6 L N S H T S T T
7cydes:
0.57 H0717B.1 A W D N N A T T
0.14 H0717B.2 F S T G R D T I
0.14 H07178.6 A W D N R D T I
0.14 H0717B.7 I Q E H N S T T
0.50 H0717E.1 F S L A N S T V
Part C:
4 cycles:
0.67 H0714C.2 F S F L K D T T
' = also contained the mutations L15R, K168R.
In Table XV below, hGH variants were selected from combinatorial libraries by the phagemid binding selection process. All hGH variants in Table XV contain two background mutations (E174SIF176Y). The number 5 0 P is the fractional occurrence of a given variant among all Gones sequenced after 4 cycles of rapid-binding selection.
' Table XV
hGH variants from RAPID hGHbp bindklp selection d en hGH-phagernld comblnatortel IIbrJry Helot Heluc wild-type residue: ~Q ~g ~$ )~ g~ p~j~
Yaf~t 0.14 H07BF4.2 W G S S R D T I
0.57 H078F4.3 M A D N N S T T
0.14 H07BF4.6 A W D N S S V T $
0.14 H078F4.7 H Q T S R D T I
$ = also contained the mutatan Y176F (wild-type hGH also contains F176j.
In table XVI below, binding constants were measured by competitive displacement of 1251-labelled hormone H06508D or labelled hGH using hGHbp (1-238) and either Mab5 or Mab263.
The variant H06508D
appears hind more than 30-fold tighter than wild-type hGH.
~~~~~J~ 5a Table XVI
EquIAbrium blndinp oo~ants of selected hGH wadaMs.
hGH Kd,(vanantl Kd,(variantl Variant Kd(H0650BD) Kd(hGH) Kd (per hGH 3 2 -1- 340 t H0650BD -1- 0.031 tOt 3 H0650BF 1.5 0.045 15 t 5 H07148.6 3.4 0.099 34 t 19 H0712B.7 7.4 0.22 74 t 30 H0712E.2 16 0.48 60 f 70 EXAMPLE XIII
Selectirve enrichment of hGH-phage contalnlng a pr~tesse substrate sequence versus ron-substrate phage As described in Example I, the plasmid pS0132 contains the gene for hGH fused to the residue Pro198 of the gene III protein with the insertion of an extra glyane residue. This plasmid may be used to produce hGH-phage particles in which the hGH~ene III fusion product is displayed monovalently on the phage surtace (Example IV). The fusion protein comprises the entire hGH protein fused to the carboxy terminal domain of gene III via a flexible linker sequerxe.
3 0 To investigate the feasibility of using phage display technology to select favourable substrate sequences for a given proteolytic enzyme, a genetically engineered variant of subtilisin BPN' was used. (Carter, P.
et al., Proteins: Structure, function and genetics 6:240-248 (1989)). This variant (hereafter referred to as A64SAL subtilisin) contains the following mutations: Ser24Cys, His64Ata, GIu156Ser, GIy169A1a and Tyr217Leu. Since this enzyme lacks the essential catalytic residue His64, its substrate speaficity is greatly restricted so that certain histidine-containing substrates are preferentially hyrdrolysed (Carter et al., Science 237:394-399 (1987)).
The sequence of the linker region in pS0132 was mutated to create a substrate sequence for A64SAL
subtilisin, using the oligonucleotide 5'-TTC-GGG-CCC-TTC-GCT-GCT-CAC-TAT-ACG-CGT-CAG-TCG-ACT-GAC-CTG-CCT-3'. This resulted in the introduction of the protein sequence Phe-Gly-Pro-Phe-Ala-Ala-5 His-Tyr-Thr-Arg-Gln-Ser-Thr-Asp in the linker region between hGH and the carboxy terminal domain of gene III, where the first Phe residue in the above sequence is Phe191 of hGH. The sequence Ala-Ala-His-Tyr-Thr-Agr-Gln is known to be a good substrate for A64SAL subtilisin (Carter et al (1989), supra). The resulting plasmid was designated pS0640.
Phagemid particles derived from pS0132 and pS0640 were constructed as described in Example I. In initial experiments, a 10W aliquot of each phage pool was separately mixed with 30p1 of oxirane beads (prepared as described in Example II) in 100W of buffer comprising 20mM Tris-HCI pH 8.6 and 2.5M NaCI. The binding and washing steps were performed as described in example VII. The beads were then resuspended in 400p1 of the same buffer, with or without 50nM of A64SAL subtilisin. Following incubation for 10 minutes, the supernatants were collected and the phage titres (cfu) measured. Table XVII shows that approximately 10 times more substrate-containing phagemid particles (pS0640) were eluted in the presence of enzyme than in the absence of enzyme, or than in the case of the non-substrate phagemids (pS0132) in the presence or absence of enzyme. Increasing the enzyme, phagemid or bead concentrations did not improve this ratio.
In an attempt to decrease the non-speafic elution of immobilised phagemids, a tight-bir>ding variant of hGH was introduced in place of the wild-type hGH gene in pS0132 and pS0640. The hGH variant used was as described in example XI (pH0650bd) and contains the mutations PhelOAla, Mett4Trp, Hisl8Asp, His2lAsn, Arg167Asn, Asp171Ser, GIu174Ser, Phe176Tyr and IIe179Thr. This resulted in the construction of two new phagemids: pDM0390 (containing tight-Minding hGH and no substrate sequence) and pDM0411 (containing tight-binding hGH and the substrate sequence Ala-Ala-His-Tyr-Thr-Agr-Gln). The binding washing and elution protocol was also changed as follows:
(i) Binding: COSTAR 12-well tissue culture plates were coated for 16 hours with 0.5mUwell 2ug/ml hGHbp in sodium carbonate buffer pH 10Ø The plates were then incubated with lmllwell of blocking buffer (phosphate buffered saline (PBS) containing 0.1%w/v bovine serum albumen) for 2 hours and washed in an assay buffer containing lOmM Tris-HCI pH 7.5, 1 mM EDTA and 1 OOmM NaCI. Phagemids were again prepared as described in Example I: the phage pool was diluted 1:4 in the above assay buffer and 0.5m1 of phage incubated per well for 2 hours.
WO 92/09690 ~. ~ PCT/US91/09133 ~~~~~~3 (ii) Washing: The plates were washed thoroughly with PBS + 0.05% Tween 20 and incubated for 30 minuted with 1 ml of this wash buffer. This washing step was repeated three times.
(~i) Eution: The plates were incubated for 10 minutes in an elution buffer consisting of 20mM Tris-HCI pH 8.6 + 100mM NaCI, then the phage were eluted with 0.5m1 of the above buffer with or without 500nM of A64SAL subtilisin.
Table XVII shows that there was a dramatic increase in the ratio of specifically eluted substrate-phagemid particles compared to the method previously described for pS0640 and pS0132. ft is likely that this is due to the fact that the tight-binding hGH
mutant has a significantly slower off-rate for binding to hGH binding protein compared to wild-type hGH.
Table XVII
Specific elution of substrate-phagemlds by A64SAL subtllisln Colony forming units (cfu) were estimated by plating out 10.1 of 10-fold dilutions of phage on l0pl spots of XL-1 blue cells, on LB agar plates containing 50pg/ml carbenicillinl (i) wld-type hGH gene: binding to hGHbp-oxirane beads pS0640 (substrate) 9x106cfu/l0wl 1.5x106cfu/l0pl pS0132 (non-substrate) 6x105cfu/l0pl 3x105cfu/l0pl (ii) pH0650bd mutant hGH gene: Minding to hGHbp-coated plates pDM0411 (substrate) 1.7x105cfu/l0pl 2x103cfu/l0pl pDM0390 (non-substrate) 2x103cfu/l0pl tx103cfu/l0pl Example XIV
Identification of preferred substrates for A64SAL subtllisln using selective enrichment of a library of substrate sequences.
We sought to employ the selective enrichment procedure described in Example XIII to identify good substrate sequences from a library of random substrate sequences.
We designed a vector suitable for introduction of randomised substrate cassettes. and subsequent expression of a library of substrate sequences. The starting point was the vector pS0643, described in Example VIII. Site-directed mutagenesis was carried out using the oligonucleotide 5'-AGC-TGT-GGC-TTC-GGG-CCC-GCC~CC-GCG-TCG-ACT-GGC-GGT-GGC-TCT-3', which introduces ~ (GGGCCC) and ~,[ (GTCGAC) restriction sites between hGH
and Gene III. This new construct was designated pDM0253 (The actual sequence of pDM0253 is 5'-AGC-TGT-GGC-TTC-GGG-CCC-GCC-ACC-GCG-TCG-ACT-GGC-GGT-GGC-TCT-3', where WO 92/09690 ~~ ~? ~ ~, PCT/US91 /09133 57 ~~~5~~1~5 the underlined base substitution is due to a spurious error in the mutagenic oligonucleotide).
In addition, the tight-binding hGH variant described in example was introduced by exchanging a fragment from pDM0411 (example XII I) The resurting library vector was designated pDM0454.
To introduce a library cassette, pDM0454 was digested with Apal folbwed by Sall, then precipitated with 13% PEG 8000+ lOmM MgCl2, washed twice in 70% ethanol and resuspended This effidently precipitates the vector but leaves the small Apa-Sal fragment in solution (Paithankar, K. R. and Prasad, K. S. N., Nuceic Acids Research 19:1346). The product was run on a 1% agarose gel and the Apal-Sall digested vector excised, purified using a Bandprep kit (Pharmacia) and resuspended for Ngation with the mutagenic cassette.
The cassette to be inserted contained a DNA sequence similar to that in the linker region of pS0640 and pDM0411, but with the colons for the histidine and tyrosine residues in the substrate sequence replaced by randomised colons. We chose to substitute NNS
(N=G/A/T/C; S=G/C) at each of the randomised positions as described in example VIII. The oligonucleotides used in the mutagenic cassettes were: 5'-C-TTC-GCT-GCT-NNS-NNS-ACC-CGG-CAA-3' (coding strand) and 5'-T-CGA-TTG-CCG-GGT-SNN-SNN-AGC-AGC-GAA-GGG-CC-3' (non-coding strand). This cassette also destroys the Sall site, so that digestion with Sall may be used to reduce the vector background. The oGgonucleotides were not phosphorylated before insertion into the Apa-Sal cassette site, as it was feared that subsequent oGgomerisation of a small population of the cassettes may lead to spurious results with multiple cassette inserts. Following annealing and ligation, the reaction products were phenol:chloroform extracted, ethanol precipitated and resuspended in water.
Initially, no digestion with Sall to reduce the background vector was pertormed.
Approximately 200ng was electroporated into XL-1 blue cells and a phagemid library was prepared as described in example VIII.
Selection of hlyhly cleavable substrates from the snb_c_trate Ilbrarv The selection procedure used was identical to that described for pDM0411 and pDM0390 in example XIII. After each round of selection, the eluted phage were propagated by transducing a fresh culture of Xl_-1 blue cells and propagating a new phagemid library as described for hGH-phage in example VIII. The progress of the selection procedure was monitored by measuring eluted phage titres and by sequencing individual clones after each round of selection.
Table A shows the successive phage titres for elution in the presence and absence of enzyme after 1, 2 and 3 rounds of selection.
2~~~~33 58 Clearly, the ratio of specifically eluted phage: non-specifically eluted phage (ie phage eluted with enzyme:phage eluted without enzyme) increases dramatically from round 1 to round 3, suggesting that the population of good substrates is increasing with each round of selection.
Sequencing of 10 isolates from the starting library showed them all to consist of the wild-type pDM0464 sequence. This is attributed to the fact that after digestion with Apal, the Sall site is very close to the end of the DNA fragment, thus leading to bw effiaency of digestion. Nevertheless, there are only 400 possible sequences in the library, so this population should still be well represented.
Tables B1 and B2 shows the sequences of isolates obtained after round 2 and round 3 of selection. Affer 2 rounds of selection, there is clearly a high incidence of histidine residues. This is exactly what is expelled: as described in example XIII, A64SAl_ subtilisin requires a histidine residue in the substrate as it employs a substrate-assisted catalytic mechanism. After 3 rounds of selection, each of the 10 Gones sequenced has a histidine in the randomised cassette. Note, however, that 2 of the sequences are of pDM0411, which was not present in the starting library and is therefore a contaminant.
WO 92/09690 ~ ~ ~ ~ ~ ~ ~ PCT/US91 Table A
Titration of Initialphape pools and phage from 3 rounds of eluted selective enrichment Colony forming units (cfu) were estimated by plating out 101,1.1 of 10-fold dilutions of phage on 101~J spots of XL-1 aining 501ig/ml carbenicillin blue cells, on LB
agar plates cont Starting library: 3x1012 cfu/ml LIBRARY: +500nM A64SAL : 4x103 cfu/101~I
no enzyme : 3x103 cfu/101~1 pDM0411: +500nM A64SAL : 2x106 cfu/10111 (control) no enzyme : 8x103 cfu/101~I
Round 1 library: 7x1012 cfu/ml LIBRARY: +500nM A64SAL : 3x104 cfu/l0wl no enzyme : 6x103 cfu/101~1 pDM0411: +500nM A64SAL : 3x106 cfu110111 (control) no enzyme : 1.6x104 cfu/101~I
Round 2 library: 7x1011 cfu/ml LIBRARY: +500nM A64SAL : 1x105 cfu/10111 no enzyme : <103 cfu/l0wl pDM0411: +500nM A64SAL : 5x106 cfu/101~I
(control) no enzyme : 3x104 cfu/101~1 so Table B1 Sequences of eluted phage after 2 rounds of selective enrichment.
All protein sequences should be of the form AA"TRO, where ' represents a randomised colon. In the table below, the randomised colons and amino acids are underlined and in bold.
After round 2:
A A $ Y T R Q
... GCT GCT~~~ ACC CGGCAA ... 2 TAC
A A $ j~j T R Q
... GCT GCT ACC CGGCAA ... 1 A A ji $ T R Q
... GCT GCT~ ACC CGGCAA ... 1 A A Z $ T R Q
... GCT GCT~ ACC CGGCAA ... 1 A A $ T R Q
.. . GCT~~ CGG CAA... 1 #
GCT
A A ~ $ T R Q
... GCT GCT~~~ ACC CGGCAA 1 ##
CAC
... wild-type 3 pDM0454 # - spurious deletion of 1 colon within the cassette ## - ambiguous sequence ~~~~~~3 Table B2 Seauences of eluted ohaqe after 3 rounds of selective enrichment.
All protein represents sequences should a be of the form AA"TRQ, where ' randomised colon.the table below, the randomised In colons and amino acids are underlined and in bold.
After round 3:
nce o-of Seau occurrences A A $ ~ T R Q
... GCT GCT ACG CGT CAG ... 2 ~
A A ji $ T R Q
... GCT GCT CAC ACC CGG CAA ... 2 CTC
2O A A Q $ T R Q
... GCT GCT ACC CGG CAA ... 1 ~
A A T $ T R Q
... GCT GCT ACC CGG CAA ... 1 A A $ ,~, R Q
... GCT GCT TCC CGG CAA ... 1 CAC
A A $ $ T R Q
... GCT GCT ACC CGG CAA 1 ~
A A $ $ R Q
... GCT GCT TTC CGG CAA ... 1 CAC
A A $ T R Q
... GCT GCT CGG CAA ... 1 # - contaminating sequence from pDM0411 ## - contains "illegal" colon CAT - T should the not appear in the 3rd position of a colon.
SEQUENCE LISTING
(1) GENERAL INFORMATION:
(i) APPLICANT: Genentech, Inc.
Garrard, Lisa J.
Henner, Dennis J.
Bass, Steven Greene, Ronald Lowman, Henry B.
Wells, James A.
Matthews, David J.
(ii) TITLE OF INVENTION: Enrichment Method For Variant Proteins With Altered Binding Properties (iii) NUMBER OF SEQUENCES: 27 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Genentech, Inc.
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(A) APPLICATION NUMBER: 07/621667 (B) APPLICATION DATE: 03-Dec-1990 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: Benson, Robert H.
(B) REGISTRATION NUMBER: 30,446 (C) REFERENCE/DOCKET NUMBER: 645P4 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: 415/266-1489 (B) TELEFAX: 415/952-9881 (C) TELEX: 910/371-7168 (2) INFORMATION FOR SEQ ID NO:1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO: l:
(2) INFORMATION FOR SEQ ID N0:2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:2:
(2) INFORMATION FOR SEQ ID N0:3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:3:
(2) INFORMATION FOR SEQ ID N0:4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 30 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:4:
(2) INFORMATION FOR SEQ ID N0:5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:5:
(2) INFORMATION FOR SEQ ID N0:6:
(i) SEQUENCE CHARACTERISTICS:
~a~5~~~
(A) LENGTH: 23 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:6:
(2) INFORMATION FOR SEQ ID N0:7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:7:
(2) INFORMATION FOR SEQ ID N0:8:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 63 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:8:
(2) INFORMATION FOR SEQ ID N0:9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 24 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9:
(2) INFORMATION FOR SEQ ID NO:10:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:
~~'~~~~3 (2) INFORMATION FOR SEQ ID NO:11:
5 (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:
(2) INFORMATION FOR SEQ ID N0:12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:12:
(2) INFORMATION FOR SEQ ID N0:13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 12 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:13:
Gly Ser Cys Gly Phe Glu Ser Gly Gly Gly Ser Gly (2) INFORMATION FOR SEQ ID N0:14:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 27 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:14:
(2) INFORMATION FOR SEQ ID N0:15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 38 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:15:
n N ~ eJ Ey 'L
(2) INFORMATION FOR SEQ ID N0:16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 30 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:16:
(2) INFORMATION FOR SEQ ID N0:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:17:
(2) INFORMATION FOR SEQ ID N0:18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:18:
(2) INFORMATION FOR SEQ ID N0:19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:
(2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
(2) INFORMATION
FOR
SEQ
ID
N0:21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:21:
TCGAGGCTCN NSGACAACGC GNNSCTGCGT GCTNNSCGTC TTNNSCAGCT
(2) INFORMATION
FOR
SEQ
ID
N0:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:22:
GTGTCAAAGG CCAGCTGSNN AAGACGSNNA GCACGCAGSN NCGCGTTGTC
(2) INFORMATION
FOR
SEQ
ID
N0:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:23:
GTTACTCTAC TGCTTCNNSA AGGACATGNN SAAGGTCAGC NNSTACCTGC
(2) INFORMATION
FOR
SEQ
ID
N0:24:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear WO 92/09690 PC1'/US91/09133 209~~~~
(xi) SEQUENCE DESCRIPTION:SEQ ID
N0:24:
(2) INFORMATION FOR SEQ
ID N0:25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2178 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION:SEQ ID
N0:25:
~J ~
~~~
~
(2) INFORMATION
FOR
SEQ
ID
N0:26:
(i) SEQUENCE
CHARACTERISTICS:
(A) acids LENGTH:
amino 20 (B) amino TYPE: acid (D) linear TOPOLOGY:
(xi) SEQID
SEQUENCE N0:26:
DESCRIPTION:
25 Met LysLysAsn IleAlaPhe LeuLeuAla SerMetPhe ValPhe Ser IleAlaThr AsnAlaTyr AlaAspIle GlnMetThr GlnSer Pro SerSerLeu SerAlaSer ValGlyAsp ArgValThr IleThr Cys ArgAlaSer GlnAspVal AsnThrAla ValAlaTrp TyrGln Gln LysProGly LysAlaPro LysLeuLeu IleTyrSer AlaSer 40 Phe LeuTyrSer GlyValPro SerArgPhe SerGlySer ArgSer Gly ThrAspPhe ThrLeuThr IleSerSer LeuGlnPro GluAsp Phe AlaThrTyr TyrCysGln GlnHisTyr ThrThrPro ProThr Phe GlyGlnGly ThrLysVal GluIleLys ArgThrVal AlaAla Pro SerValPhe IlePhePro ProSerAsp GluGlnLeu LysSer 55 Gly ThrAlaSer ValValCys LeuLeuAsn AsnPheTyr ProArg Glu AlaLysVal GlnTrpLya ValAspAsn AlaLeuGln SerGly Asn SerGlnGlu SerValThr GluGlnAsp SerLyaAsp SerThr Tyr SerLeuSer SerThrLeu ThrLeuSer LysAlaAsp TyrGlu Lys HisLysVal TyrAlaCys GluValThr HisGlnGly LeuSer Ser Pro Val Thr Lys Ser Aen ArgGly GluCys Phe (2) INFORMATION FOR SEQ
ID N0:27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 461 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION:SEQ ID
N0:27:
Met Lys Lys Asn Ile Ala Leu LeuAla SerMet PheVal Phe Phe Ser Ile Ala Thr Asn Ala Ala GluVal GlnLeu ValGlu Tyr Ser Gly Gly Gly Leu Val Gln Gly GlySer LeuArg LeuSer Pro Cys Ala Ala Ser Gly Phe Asn Lys AspThr TyrIle HisTrp Ile Val Arg Gln Ala Pro Gly Lys Leu GluTrp ValAla ArgIle Gly Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lye Gly Arg Phe Thr IleSerAla AspThrSer LysAsn ThrAlaTyr LeuGln Met Asn SerLeuArg AlaGluAsp ThrAla ValTyrTyr CysSer Arg Trp GlyGlyAsp GlyPheTyr AlaMet AspTyrTrp GlyGln Gly Thr LeuValThr ValSerSer AlaSer ThrLysGly ProSer Val Phe ProLeuAla ProSerSer LysSer ThrSerGly GlyThr Ala Ala LeuGlyCys LeuValLys AspTyr PheProGlu ProVal Thr Val SerTrpAsn SerGlyAla LeuThr SerGlyVal HisThr Phe Pro AlaValLeu GlnSerSer GlyLeu TyrSerLeu SerSer Val Val ThrValPro SerSerSer LeuGly ThrGlnThr TyrIle Cys Asn ValAsnHis LysProSer AsnThr LysValAsp LysLys Val Glu ProLysSer CysAspLys ThrHis ThrGlyPro PheVal Cys Glu TyrGlnGly GlnSerSer AspLeu ProGlnPro ProVal Asn Ala GlyGlyGly SerGlyGly GlySer GlyGlyGly SerGlu Gly GlyGly SerGlu GlyGlyGly SerGluGly GlyGlySer Glu Gly GlyGly SerGly GlyGlySer GlySerGly AspPheAsp Tyr Glu LysMet AlaAsn AlaAsnLys GlyAlaMet ThrGluAsn Ala Asp GluAsn AlaLeu GlnSerAsp AlaLysGly LysLeuAap Ser Val AlaThr AspTyr GlyAlaAla IleAspGly PheIleGly Asp Val SerGly LeuAla AsnGlyAsn GlyAlaThr GlyAspPhe Ala Gly SerAsn SerGln MetAlaGln ValGlyAsp GlyAspAsn Ser Pro LeuMet AsnAsn PheArgGln TyrLeuPro SerLeuPro Gln Ser ValGlu CyaArg ProPheVal PheSerAla GlyLysPro Tyr Glu PheSer IleAsp CysAspLys IleAsnLeu PheArgGly Val Phe AlaPhe LeuLeu TyrValAla ThrPheMet TyrValPhe Ser Thr PheAla AsnIle LeuArgAsn LysGluSer
wash), beads were suspended in Glycine buffer (Buffer A plus 0.2 ~Glycine, pH
2.0 with HCI), rotated t hour and pelleted. The supernatant ('Glyane elution'; ZOOR.L) was neutralized by adding 30 ml of 1 M Tris base and stored at 4o C.
5. PROPAGATION: Aliquots from the hGH elutions and from the Glyane elutions from each set of 15 beads under each set of conditions were used to infect separate cultures of log-phase XLt-Blue cells.
Transductions were carried out by mixing phage with 1 mL XLt-Blue cells, incubating 20 min. at 37oC, then adding K07 (moi= t00). Cultures (25 mL 2YT plus carbenicillin) were grown as described above and the next pool of phage was prepared as described above.
Phage binding, elution, and propagation were carried out in successive rounds, according b the cycle 20 described above. For example, ~e phage amplified from the hGH elution from hGHbp-beads were again selected on hGHbp-beads and eluted with hGH, then used to infect a new culture of XL1-Blue cells. Three to five rounds of selection and propagatan were carried out for each of fhe selection procedures described above.
From the hGH and Glyane elution steps of each cycle, an aliquot of phage was used to inoculate XLt-Btue 25 cells, which were plated on LB media containing carbenialGn and tetracyGine to obtain independent doves from each phage pool. Single-stranded DNA was prepared from isolated cobny and sequenced in the region of the mutagenic cassette. The results of DNA sequencing are summarized in terms of the deduced amino acid sequerx;es in Figures 5, 6, 7, and 8.
3 0 ~.i~B~,h~H,~
To determine the tHnding affinity of some of the selected hGH mutants for the hGHbp, we transformed DNA from sequenced clones into ~ strain 1609. As described above, this is a non-suppressor strain which terminates translation of protein after the final Phe-191 residue of hGH.
Single-stranded ONA was used for these transformations, but double-stranded DNA or even wtrole phage can be easily electroporated into a non-35 suppressor strain for expression of free hormone.
Mutants of hGH were prepared from osmotically shocked cells by ammonium sulfate precipitation as described for hGH (Olson et al., ~g 293, 408-41 t (1981)), and protein concentrations were measured by laser densitomoetry of Coomassie-stained SDS-polyacrylamide gel electrophoresis gels, using hGH as standard (Cunningham and Wells, 244, 1081-1085 (1989)).
The binding affinity of each mutant was determined by displacement of 1251 hGH
as described (Spencer et al., J. Biol. Chem. 263, 7862-7867 [1988] ; Fuh et al., J. Biol. Chem. 265, 3111-3115 [1990]), using an anti-receptor monoclonal antibody (Mab263).
The results for a numt~er of hGH mutants, selected by different pathways (Fig.
6) are shown in Table VII. Many of these mutants have a tghter binding affinity for hGHbp than wild-type hGH. The most improved mutant, KSYR, has a binding affinity 5.6 times greater than that of wed-type hGH. The weakest selected mutant, among those assayed was only about 10-fold lower in binding affinity than hGH.
Binding assays may be carried out for mutants selected for hPRlbp-binding.
Table VII.
Cotre bhdhg b tlGiibP
The selected pool in which each mutant was found is indicated as 1 G (first glyclne selection), 3G (third glycine selection), 3H (third hGH selection), 3' (third selection, not tlinding to hPRI_bp, but Minding to hGHbp).
The number of times e~h mutant ocLKrred among all sequerxed Bones is shown ().
Mutant Kd (nM) Kd(mut)/Kd(hGH) Pod KSYR (6) 0.06 + 0.01 0.18 1G,3G
RSFR 0.10 + 0.05 0.30 3G
RAYR 0.13 + 0.04 0.37 3' KTYK (2) 0.16 + 0.04 0.47 H,3G
RSYR (3) 0.20 + 0.07 0.58 ~ 1G,3H,3G
KAYR (3) 0.22 + 0.03 0.66 3G
RFFR (2) 0.26 + 0.05 0.76 3H
KQYR 0.33 + 0.03 1.0 3G
KEFR= wt (9) 0.34 + 0.05 1.0 3H,3G,3' RTYH 0.68 + 0.17 2.0 3H
QRYR 0.83 + 0.14 2.5 3' KKYK 1.1 +0.4 3.2 3' RSFS (2) 1.1 + 0.2 3.3 3G,' KSNR 3.1 + 0.4 9.2 3' At some residues, substitution of a particular amino acrd has essentially the same effect independent of surrounding residues. For example. substitution of F176Y in the background of 172Rl174S redtxes tHnding affinity by 2.0-fold (RSFR vs. RSYR). Similarly, in the background of 172KI174A the tHnding affinity of the F176Y mutant (KAYR) is 2.9-fold weaker than the corresponding 176F mutant (KAFR; Cunningham and Wells, 1989).
WO 92/09690 ~ ~ ~ ~ ~ ~ ~ PCT/US91 /09133 On the other hand, the binding constants determined for several selected mutants of hGH demonstrate ran~additive etfeds of some amino aad substitutions at residues 172,174,176, and 178. For example, in the background of 172KI176Y, the substitution E174S results in a mutant (KSYR) which binds hGHbp 3.7-fold tighter than the corresponding mutant containing E174A (KAYR). However, in the background of 172R/176Y, the effects of these E174 substitutions are reversed. Here, the E174A mutant (RAYR) binds 1.5-fold tighter than the E174S mutant (RSYR).
Such non~additive effects on binding for substitutions at proximal residues illustrate the utility of protein-phage binding selek~ion as a means of selecting optimized mutants from a library m~ndomized at several positions. ~ the absence of deta~ed swdural information, without such a selection process, many combinations of substitutions might be tried before finding the optimum mutant.
EXAMPLE IX
SELECTION OF hGH VARIANTS FROM A HELIX~1 RANDOM CASSETTE
LIBRARY OF HORMONE-PHAGE
Using the methods described in Example VIII, we targeted another region of hGH
involved in binding to the hGHbp andlor hPRLbp, helix 1 residues 10,14,18, 21, for random mutagenesis in the phGHam~3p vector (also known as pS0643; see Example VIII).
We chose to use the'amber' hGH-g3 construct (called phGHam~g3p) because it appears to make the target protein, hGH, more accessible fx binding. This is supported by data from comparative ELISA assays of monodonaJ antibody binding. Phage produced from both pS0132 (S. Bass, R.
Greene, J. A. Wells, Proteins 8, 309 (1990).) and phGHam-g3 were tested with three antibodies (Medix 2,1 B5.G2, and 5B7.C10) that are known to have binding detertninar>is near the carboxyl-terminus of hGH [B. C.
Cunningham, P. Jhurare, P. Ng, J. A. Wells, Saenee 243,1330 (1989); B. C. Cunningham and J. A. Wells, Silence 244,1081 (1989); L. Jin and J. Wells, unpublished results], and one antibody (Medix 1 ) that recognizes determinants in helices 1 and 3 ([B. C.
Cunningham, P. Jhurani, P. Ng, J. A. Wells, Saerx;e 243,1330 (1989); B. C.
Cunringham and J. A. Wells, Science 244,1081 (1989)]). Pt~agemid partides from phGHam-g3 reacted much more strongly with antibodies Medix 2, 185.62, and 5B7.C10 than did phagemid partiGes from pS0132. In particular, binding of pS0132 particles was reduced by >2000-fold for both Medix 2 and 5B7.C10 and reduced by >25-fold for 1B5.G2 compared to binding to Medix 1. On the other hand, binding of phGHam~3 phage was weaker by only about 1.5-fold,1.2-fold, and 2.3-fold for the Medix 2,1 B5.G2, and 587.C10 antibodies, respectively, compared with binding to MEDIX 1.
We mutated residues in helix 1 that were previously identified by alar>ine-scanning mutager~esis (B. C.
Cunr>rngham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989); B. C.
Cunr>ingham and J. A. Wells, Science 244, 1081 (1989),15, 16) to modulate the binding of the extracellular domains of the hGH andlor hPRL
receptors (called hGHbp and hPRLbp, respectively). Cassette mutagenesis was varied out essentially as described [J. A. Wells, M. Vasser, D. B. Powers, Gene 34, 315 (1985)]. This library was constructed by cassette mutagenesis that fully mutated four residues at a time (see Example VIII) which utilized a mutated version of phGHam-g3 into which unique Kpnl (at hGH codon 27jand Xhol (at hGH codon 6) restriction sites (underlined below) had been inserted by mutagenesis [ T. A Kunkel, J. D. Roberts, R. A
Zakour, Methods EnzymoL 154, 367-2~9~~~3 38 382] with the oligonucleotides 5'-GCC TTT GAC AGG TAC CAG GAG TTT G-3' and 5'-CCA ACT ATA CCA
CTS TCG AGG TCT ATT CGA TAA C-3', respectively. The later oligo also introduced a +1 frameshift (italidzed) to terminate translation from the starting vector and minimize wild-type background in the phagemid library. This strafing vector was designated pH0508B. The helix 1 library, which mutated hGH residues 10, 14, 18, 21, was constructed by ligating to the large Xhol-Kpnl fragment of pH0508B
a cassette made from the complementary oiigonucleotides 5'-pTCG AGG CTC NNS GAC AAC GCG NNS CTG CGT GCT
NNS CGT CTT
NNS CAG CTG GCC TTT GAC ACG TAC-3' and 5'-pGT GTC AAA GGC CAG CTG SNN AAG ACG
SNN AGC
ACG CAG SNN CGC GTT GTC SNN GAG CC-3'. The Kpnl site was destroyed in the junction of the ligation product so that restriction enzyme digestion could be used for analysis of non-mutated background.
The library contained at least 10~ indeperkient transfonnaMs so that if the library were absolutely random ( 106 different combinations of colons) we would have an average of about 10 copies of each posside mutated hGH gene. Restriction analysis using Kpni indicated that at least 80%
of helix 1 library constructs contained the inserted cassette.
Binding enrichments of hGH-phage from the libraries was carried out using hGHbp immobilized on oxirane-polyacrylamide beads (Sigma Chemical Co.) as described (Example VIII).
Four residues in helix 1 (F10, M14, H18, and H21 ) were similarly mutated and after 4 and 6 cycles a non-wild-type consensus developed (Table VIII). Position 10 on the hydrophobic face of helix 1 tended to be hydrophobic whereas positions 21 and 18 on the hydrophillic face tended were dominated by Asn; no obvaus consensus was evident for position 14 (Table IX).
The binding constants for these mutants of hGH to hGHbp was determined by expressing the free hormone variants in the non-suppressor E. coli strain 1609, purifying the protein, and assaying by competitive displacement of labelled wt-hGH irom hGHbp (see F~cample VIII). As indicated, several mutants bind tighter to hGHbp than does wt-hGH.
Table VIII.
Selection of hGli helix 1 mutants Variarns of hGH (randomly mutated at residues F10, Mt4, H18, H21) expressed on phagemid particles were selected by binding to hGHbp-beads and eluting with hGH (0.4 mIN) buffer followed by gfycine (0.2 M, pH 2) buffer (see Example VIII).
Gly elution 4 Cycles H G N N
A W D N (2) Y T V N
I N I N
L N S H
F S F G
6 Cycles H G N N (6) F S F L
Consensus:
H G N N
~~95~3~ 40 Table IX
Ca~enst8 sequels tram the selected helix 1 Nbrary Observed frequency is tn~tion of all doves sequerxed with the indicated amino aad. The nominal frequency is cabulated on the basis of NNS 32 colon degeneracy. The maximal enrichment facto varies from 11 ~ 32 depending upon the rwminal frequency value for a given residue. Values of [Kd(Ala mut)IK d(wt hGH)J for single alanine mutations ware taken from B. C. Cumingham and J. A. Wells, Sderx~e 244,1081 (1989); B. C. Cunningham, D. J. Henner, J. A. Wells, Soaves 247,1461 (1990); B. C. Cunningham and J. A.
Wells, Proc. Nail. Acad Sa. USA
88, 3407 (1991 ).
wld type Selected Kd(Ala mut) residue Kd(wt hGH) re~due observed rornir~al Enrichment F10 5.9 H 0.50 0.031 17 F 0.14 0.031 5 A 0.14 0.062 2 M14 2.2 G 0.50 0.062 8 W 0.14 0.031 5 N 0.14 0.031 5 S 0.14 0.093 2 H18 1.6 N 0.50 0.031 17 D 0.14 0.031 5 F 0.14 0.031 5 H21 0.33 N 0.79 0.031 26 H 0.07 0.031 2 Table X
t8lndnp d puri~d hGH helix 1 rt~nts b hGHbp Competition Minding experiments were performed using [l~IjhGH (wild-type), hGHbp (containing the extracellular receptor domain, residues 1-238), and Mab263 [B. C. Cumingham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989));. The number P indicates the fractional ocaxrence of each mutant among all the clones sequenced after one or more rounds of selection.
Sequence P Kd (nA~\f(Kd Kd(wt pogtion mut) hGH)) H G N N 0.50 0.14 t 0.04 0.42 A W D N 0.14 0.100.03 0.30 wt= F M H H 0 0.34 0.05 (1 ) F S F L 0.07 0.680.19 2.0 Y T V N 0.07 0.750.19 2.2 L N S H 0.07 0.82 t 0.20 2.4 I N I N 0.07 12 ~ 0.31 3.4 EXAMPLE X
SELECTION OF hGH VARIANTS FROM A HEUX-4 RAN00M CASSETTE LIBRARY CONTAINING
Our experience with reauiting non-binding homologs of hGH evolutionary variants suggests that many individual amino acid substitutions can be combined to yield cumulatively improved mutants of hGH with respect to binding a particular receptor (B. C. Cunnirgham, D. J. Henner, J. A. Wells, Scien~oe 247,1461 (1990); B. C.
Cunningham and J. A. Wells, Pros. Nad. Acad Sa. USA 88, 3407 (1991 ); H. 8.
Lowman, B. C. Cumingham, J. A.
3 5 Wells, J. 8iol. Chem. 266, in press ( 1991 )j.
The helix 4b library was constructed in an attempt to further improve the helix 4 double mutant (E174SIF176Y) selected from the helix 4a library that we found bound tighter to the hGH receptor (see Example VIII). With the E174S/F176Y hGH mutant as the background starting hormone, residues were mutated that surrounded positions 174 and 176 on the hydrophiNc face of helix 4 (R167, D171, T175 and 1179) .
Cassette mutagenesis was carried out essentially as described [J. A. Wells. M.
Vasser, D. B. Powers, Gene 34, 315 (1985)). The helix 4b library, which mutated residues 167,171,175 and 179 within the E174S/F176Y background, was constructed using cassette mutagenesis that fully mutated four residues at a time (see Example VIII) and which utilized a mutated version of phGHam~3 into which uryque BstBl and BgAI
restriction sites had been inserted prevausly (Example VIII). into the BstEll-Bglll sites of the vector was inserted a cassette made from the complementary oligonudeotides 5'-pG TTA CTC TAC TGC
TTC NNS AAG GAC ATG
NNS AAG GTC AGC NNS TAC CTG CGC NNS GTG CAG TGC A-3' and 5'-pGA TCT GCA CTG
CAC SNN
GCG CAG GTA SNN GCT GAC CTT SNN CAT GTC CTT SNN GAA GCA GTA GA-3'. The BstEfl site was eliminated in the ligated cassette. From the helix 4b litxary,15 unselectsd doves were sequenced. Of these, none lacked a cassette insert, 20% were frame-shifted, and 7% had a non-silent mutatan.
Binding enrichments of hGH-phage from the libraries was carried out using hGHbp immobilized on oxirane-polyaaylamide beads (Sigma Chemical Co.) as described (Example VIII).
After 6 cycles of binding a reasonably dear consensus developed (Table XI). Interestingly, all positions tended to contain polar residues, notably Ser, Thr and Asn (XII).
The binding constants for some of these mutants of hGH to hGHbp was determined by expressing the free hormone variants in the non-suppresser E. cell strain 16C9, purifying the protein, and assaying by competitive displacement of labelled wtfiGH from hGHbp (see Example VIII). As indicated, the binding affinities of several helix-4b mutants for hGHbp were tighter than that of wt-hGH Table XIII).
Finally, we have begun to analyze the binding affinity of several of the tghter hGHbp binding mutants for their ability to bind to the hPRLbp. The E174S/F176Y mutant binds 200-fold weaker to the hPRLbp than hGH. The E174T/F176YIR178K and R167N/D171SIE174S/FI76YII179T mutants each bind >500-fold weaker to the hPRLbp than hGH. Thus, it is possible be use the produce new receptor selective mutants of hGH by phage display tectx~ology.
Of the 12 residues mutated in three hGH-phagemid libraries (Examples VIII, lX, X), 4 showed a strong, although not exclusive, conservation of the wild-type residues (K172, T175, F176, and R178). Not surprisingly, these were residues that when converted to Ata caused the largest disruptions (4- to 60-fold) in binding affinity to the hGHbp. There was a class of 4 other residues (F10, M14, D171, and 1179) where Ala substitutions caused weaker effects on binding (2- to 7-fold) and these positions exhibited little wild-type consensus. Finally the other 4 residues (H18, H21, 8167, and E174), that promote binding to he hPRLbp but not the hGHbp, did not exhibit any consensus for the wild-type hGH sequence by selection on hGHbp-beads. In fact iwo residues (E174 and H21 ), where Ala substitutions enhance binding affinity to the hGHbp by 2- to 4-fold [B. C. Cunningham, P.
Jhurani, P. Ng, J. A. Wells, Sdenoe 243,1330 ( 1989); B. C. Cunningham and J.
A Wells, Scenes 244,1081 (1989); B. C. Cunr>ingham, D. J. Henner, J. A. Wells, Saerx;e 247,1461 (1990);
B. C. Cunningham and J. A, Wells, Proc. Natl. Acad. Sa. USA 88, 3407 (1991 )]. Thus, the alanine-scanning mutagenesis data correlates reasonably well with the flexibility to substitute each position. In fact , the reduction in binding affinity caused by alanine substitutions [B. C. Cunningham, P. Jhurani, P. Ng, J. A. Wells, Science 243,1330 (1989); B. C. Cunningham and J. A. Wells, Sdenoe244,1081 (1989)], B. C. Cunningham, D. J. Henner, J. A.
Wells, Science247, 1461 (1990); 8.
C. Cunningham and J. A. Wells, Pros. Natf. Acad. Sa. USA 88, 3407 ( 1991 )] is a reasonable predictor of the percentage that the wild-type residue is found in the phagemid pool after 3-6 rounds of selection. The alanine-WO 92/09690 ~ ~ ~ ~ PCT/US91/09133 scanning information is useful for targeting side-chains that modulate binding, and the phage selection is appropriate for optimizing them and defining the flexibility of each site (and/or combinations of sites) for substitution. The comt~ir~tion of scanr~rg mutational methods [B. C.
Cunningham, P. Jhurani, P. Ng, J. A. Wells, Science ?A3,1330 (1989); B. C. Cunningham and J. A Wells, Saenc~e ?A4,1081 ( 1989)] and phage display is a powerful approach to designing reoepta-ligand interfaces and sdrdying molecular evolution in vitro.
In cases where combined mutatans in hGH have additive effects on binding affinity bo receptor, mutations learned through hormone-phagemid enrichment to improve bindirg can be combined by simple cutting and ligation of resfiction fragments or mutagenesis to yield cumulatively optimized mutants of hGH.
On the other hand, mutations in one region of hGH which optimize receptor binding may be stn~cturally or functionally compatible witty mutations in an overlapping a another region of the molecule. In these cases, hormone phagemid enrichment can be cartied out by one of several variatans on the iterdtiHe emid~ment approach (t ) random DNA litxaries can be generated in each of two (or perhaps more) regions of the molecule by cassette or another mutagenesis method. Thereafter, a combined library can be created by ligation of restriction fragments from the two ONA libraries; (2) an hGH variant, optimized for binding by mutation in one region of the molecule, can be randomly mutated in a second region of the molecule as in the helix-4b library example; (3) two or more random libraries can be y selected for improved binding by hormone-phagemid enrichment; after this 'roughing-in' of the optimized binding site, the still-partially-diverse libraries can be recombined by ligation of restriction fragments Go generate a single library, partially diverse in iwo or more regions of the molecules, which in tum can be further selected for optimized binding using hormone-phagemid enrichment.
2Q9~~'a~
Table XI.
AAumnt pt~erNds d hGH sHecbed from helix 4b library after 4 and 6 cyGes of enrichment Selection of hGH helix 4b mutants (randomly mutated at residues 167, 171,175,179), each containing the E174SIF176Y
double mutant, by binding to hGHbp-beads and eluting with hGH (0.4 mA~ buffer followed by glyane (0.2 M, pH 2) buffer.
One mutant (+) contained the spurious mutation R178H.
8167 Di71 T175 1179 4 Cydes N S T T
K S T T
S N T T
D S T T
D S T T+
D S A T
D S A N
T D T T
N D T N
A N T N
A S T T
6 CyGes N S T T (2) N N T T
D S S T
E S T I
K S T L
Cor~r~s:
N S T T
D N
WO 92/09690 ~ ~ j1 ;~ ~ ~ ~ PGT/US91/09133 Tale )01 Cornell~s ~puerroes from the >teleaed library.
Observed frequency is traction of aA cbnes sequenced with the indicated amino aad. The nominal frequerxy is calculated on the basis of NNS 32 colon degeneracy. The maximal enrichment factor varies from 11 to 16 to 32 5 depending upon the raminal frequency value for a given residue. Values of [Kd(Ala mut)IKd(wt hGH)] for single alanine mutations were taken from refs. below; for position 175 we oNy have a value br the T175S mutant [B. C.
Cunningham, P. Jtxuani, P. Ng, J. A. Wells, Saenoe 243,1330 (1989); B. C.
Cumingham and J. A. Wells, Saence 244, 1081 (1989); B. C. Cunrungham, D. J. Henner, J. A. Wells, Saenoe 247,1461 (1990); B. C. Cunningfam and J. A Wells, Proc. Nail. Acad Sci. USA88, 3407 (1991).).
Wild type Selected residue Kd(~a mut) residue observed raminal Enrichment Kd(wt hGH) 8167 0.75 N 0.35 0.031 11 D 0.24 0.031 8 K 0.12 0.031 4 A 0.12 0.062 2 D171 7.1 S 0.76 0.093 8 N 0.18 0.031 6 2 0 D 0.12 0.031 ' 4 T175 3.5 T 0.88 0.062 14 A 0.12 0.031 4 1179 2.7 T 0.71 0.062 11 N 0.18 0.031 6 Table XIII
t3Ndirrg of purified trGH mutar>m to hGHbp.
Competition binding experiments were performed using [1251]hGH (wild-type), hGHbp (containing the extraceNular receptor domain, residues 1-238), and Mab263 (11). The number P
indicates the fractional oaxxrence of each mutant among aU the doves sequenced after one or more rods of selection. Note that the helix 4b mutations (") are in the background of hGH(E174SIF176Y). In the list of helix 4b mutants" the E174SIF176Y mutant ('), with wt residues at 167,171,175, 179, is shown in bold.
Kd(Ala m~) SequerxePosition P Kd(n d(w~ t hGH) N S T T 0.18 0.04 t 0.02 0.12 E S T I 0.06 0.04 0.02 0.12 K S T L 0.06 0.05 0.03 0.16 N N T T 0.06 0.06 0.03 0.17 R D T I 0 0.06 0.01 (0.18) N S T Q 0.06 0.26 0.11 0.77 WO 92/09690 r' PCT/US91/09133 2~~~~~3~~
Assembly d F~ ~Aolea~le on tie Pld Sur<aoe Ptasmid pDH 188 captains the DNA encoding the F~ portion of a humanized IgG
antibody, called 4D5, that recognizes the HER-2 receptor. This plasmid is contained in E. coN strain SR 101, and has been deposited with the ATCC in RodcviNe, MD.
Briefly, the plasmid was prepared as follows: the starting plasmid was pS0132, oonta~ing the alkaline phosphatase promobar as described above. The DNA encoding human growth hormone was exased and, after a series of manipulations to make the ends of the plasmid compatible for ligatbn, the DNA encoding 4D5 was inserted. The 4D5 DNA contains two genes. The first gene encodes the variable and constant regions of the light chap, and contains at its 5' end the DNA erxxxJing the st II signal sequence.
The second gene cor>tains four portions: first, at its 5' end is the DNA erxodMg the st II signal sequence.
This is followed by the DNA erxoding the variable domain of the heavy chain, which is folbwed by the DNA encoding the first domain of the heavy chain constant region, which in turn is iolbwed by the DNA encoding the M13 gene III. The salient features of this construct are shown in Fgure 10. The sequence of the DNA erxxding 4D5 is shown in Fgure 11.
Both polyethylene glycol (PEG) and electroporation were used to transform plasmids into SRlOt cells.
(PEG competent cells were prepared and transformed according to the method of Chung and Miller (Nucleic Acids Res.16:3580 [1988]). Cells that were competent for electroporatbn were prepared, and subsequently transformed via electroporation according to the method of Zabarovsky and Winberg (Nucleic Acids Res.18:5912 [1990]). After placng the cells in 1 ml of the SOC media (described in Sambrook et al., supra), they were grown for 1 hour at 37°C with shaking. At this time, the concentration of the cells was determined using light scattering at ODSpO. A titered K07 phage stock was added bo achieve an multiplicity of infection (M01) of 100, and the phage were albwed to adhere to the cells for 20 minutes at room temperature.
This mixture was then diluted into 25 mls of 2n broth (described in Sambrook et al., supra) and incubated with shaking at 37°C overnight. The next day, cells were pelleted by centrifugation at 5000 x g for 10 minutes, the supernatant was collected, and the phage partices were precipitated with 0.5 M NaCI and 4% PEG (final corxentration) at room temperature for 10 minutes. Phage partices were pelleted by centrifugatbn at 10,000 x g for 10 minutes, resuspended in 1 ml of TEN
(10 mM Tris, pH 7.6,1 mM EDTA, and 150 mM NaCI), and stored at 4°C.
Aliquots of 0.5 ml from a solution of 0.1 mg/ml of the extra~etlutar domain of the HER-2 antigen (ECD) or a solution of 0.5 mg/ml of BSA (control antigen) in 0.1 M sodium bicarbonate, pH 8.5 were used to coat one well of a Falcon 12 well tissue culture plate. Once the solution was applied to the wells, the plates were incubated at 4°C on a rocking plattortn overnight. The plates were then blocked by removing the initial solution, applying 0.5 ml of blocking buffer (30 mg/ml BSA in 0.1 M sodium bicarbonate), and incubating at room temperature for one hour.
Finally, the blocking buffer was removed, 1 ml of buffer A (PBS, 0.5% BSA, and 0.05% Tween-20) was added, and the plates were stored up to 10 days at 4°C before being used for phage selection.
a~
Approximately 109 phage particles were mixed with a 100-fold excess of K07 helper pfrage and 1 ml of buffer A . This mixhxe was divided irno two 05 ml aliquots; one of which was applied to ECD coated wells, and the other was applied b BSA coated weus. The plates were ina~batad at room temperature while shaking for one to ttxee hours, and were then washed three times over a period of 30 mirurtes wish 1 ml aliquots of buffer A.
Elution of the phage from the plates was done at room temperature by one of two methods: t ) an initial overnight incubation of 0.025 mglml purified Mu4D5 antibody (murine) folbwed by a 30 minute irxubation with 0.4 ml of the add elution buffer (0.2 M glydne, pH 2.1, 0.5% BSA, and 0.05% Tween-20), or 2) an incubation with the acid elution tx~ffer alone. Eluates were then neutralized with 1 M Tris vase, and a 0.5 ml aliquot of TEN was added.
These samples were then propagated, titered, and shred at 4°C.
Alquots of eluted phage were added to 0.4 ml of 2YT broth and mixed with approximately 108 mid-log phase male E. cbli strain SR101. Phage were allowed b adhere to the cells for 20 minutes at room temperature and then added to 5 ml of 2YT broth that contained 50 R.giml of carbenidllin and 5 ~g/ml of tetracycline. These cells were grown at 37°C for 4 to 8 hours until they reached mid-log phase. The OD6pp was determined, and the cells were superinfected with K07 helper phage for phage production. Orxe phage particles were obtained, they were titered in order to determine the number of colony forming urNts (cfu).
This was done by taking aliquots of serial dilutions of a given phage stock, allowing Ihem to infect mid-log phase SRt 01, and plating on LB plates oontaimg 50 uglml c~rbeniciuin.
2 0 ~ p8fllZd, The affinity of h4D5 Fab fragments and Fab phage for the ECD antigen was determined using a competitive receptor Minding RIA (Burt, D. R., Receptor Bending in Dnrg Research. 0'Brien, R.A. (Ed.). pp. 3-29, Dekker, New York [1986)). The ECD antigen was labeled with 125-Ipd;ne using the sequential c~loramine-T
method (De Larco, J. E. et al., J. Ceff. PhysioJ.109:143-152 [1981]) which produced a radioactive tracer with a speafic activity of l4~Cilf,ig and incorporation of 0.47 moles of Iodine per mole of receptor. A series of 0.2 ml solutions containing 0.5 ng (by ELISA) of F~ or F~ phage, 50 nCi of 1251 ECD
tracer, and a range of unlabeled ECD amounts (6.4 ng to 3277ng) were prepared and incx~bated at room temperature overnight. The labeled ECD-F~ or ECD-FaM phage complex was separated from the unbound labeled antigen by forming an aggregate complex induced by the addition of an anti-human IgG (Fitzgerald 40-GH23) and 6°~ PEG 8000. The complex was pelleted by centrifugation (15,000 x g for 20 minutes) and the amount of labeled ECD (in cpm) was determined by a gamma counter. The dissoaation constant (Kd) was cala~lated by employirg a modified version of the program LIGAND (Munson, P. and Rothbard, D., Anal. &ochem.107~20-239 [1980)) which utilizes Scatchard analysis (Scatchard, G.,Ann. N.Y. Acad. Sci. 51:66072 (1949]). The Kd values are shown in Figure 13.
Murine 4D5 antibody was labeled with 125-l to a spedfic activity of 40-50 ~Cilpg using the lodogen procedure. Solutions containing a constant amount of labeled antibody and increasing amounts of unlabeled variant Fab were prepared and added to near confluent cultures of SK-BR-3 cells grown in 96-well microtiter dishes (final concentration of labeled antibody was 0.1 nM). After an overnight incubation at 4°C, the supernatant was removed, the cells were washed and the cell assodated radioactivity was determined in a gamma counter. Kd wakres were determined by analyzing ltte data usirta a motifi9d version of the program t.IGAND (MunsorL P. end Rothbaro, D., supra?
This depOSit of pldsmid pDHt 89 ATCC no. 68683 was made und9r the provisions of tkte St~dapest Treaty or1 the International Recognition of the Deposit of Micraorgsnisms for the Purpose of 5 patent Procedure and the Regulations thereunder (Budapest Treaty). This assures maintenance of a viable Culture for 30 ye8rs from the date of deposit. The organisms will be made available by ATCC
under the terms of the Budapest Treaty, and subject t0 an agreement between Genentech, Inc. End AT~~, which assures permanent and unrestricted availability of the progeny of the cultures to the public upon issuance of a f $tent On the basis of the application, or the patent appllGation is refused, or i5 abandoned and no longer subject to reinstatement, or is withdrawn, whichever oomes first, and assures Q availability of the progeny t0 One determined by the Commissioner of Pntetlt5 to be entitled thereto according to Section 109 of the Patent l=lutes.
The assignee of the present application has agreed that If the cultures on deposit sttoutd d'le or be Iosi or destroyed when cultivated under suitable t>onditiats, they wiN be promptly replaced on rtotificatan wi>rt a viable 15 specimen of the same a.~ture. Availability of the deG4sibed cultures is rtot to be construed as a license to practice the invention in contravention of the rights granted under tile authority of any government in accordance with its patent laws.
The fxegdrtg written spedt'~abwt is considered to be suttident to enable one skilled in the art to practice the invention. The present ~ven>ion is not to be limited in scope by ttte cultures deposited, since the 20 deposited embodiments are Intended as separate illustrations of certain aspects at the invention and any a~tures that are ftatctionally equivalent are within fhe sa~pe of this irnrention. The deposit of material herein does not constitute an admission that the wriaen description hlereln contained is inadequate to enable the pracBce of any aspect of the invention, including the best mode thereof, nor is it to be CansUtAad as limiting the soop2 of tt"to dairns to the specific illustrations that it represents. Indeed, Parlous modifications of the irnention in addition to 25 those shown 2ind descrit~ed herein wiU become apparent to those skilled in tile an from the foregoing desaipCioh and fall within me scope of the appended Balms.
whaa ttte irrrer~tion has neoessaray been desaitied in coftjurtction wilt prelerted embodiments, one of ordinary skin, after reading the toregaing speafication, w~l be able to etrect various changes, substitutions of equivalents, and alterations de Ute sut~ed rnatter set forth herein, widtout departing lrom the spirit and scone 34 thereof. Hence, the invention can be practiced in ways outer than those speafica~y desaibed herein. ft is therefore intended that the protetdon granted by ~et4ars Patent hereon be mired artly by the appended claims and equivalents thereof.
WO 92/09690 '~ ~ ~ f; ~' j ~ PCT/US91 /09133 EXAMPLE Xll SELECTION OF hGH VAi'~ANTS FROM COMBINATIONS OF HELIX-1 AND HEUX-4 HORMONE-PHAGE
VARIANTS
According to additivity principles ~J. A. Wells, Biochemistry29, 8509 (1990)j, mutations in different parts of a protein, if they are not mutually interacting, are expected to combine to produce additive changes in the free energy of Minding to another molecule (changes are additive in terms of AOGb;~ing, or multiplicative in terms of Kd = exp[-eGIRTj ). Thus a mutation produang a 2-fold increase in binding affinity, when comtHned with a second mutation causing a 3-fold increase, would be predicted to yield a double mutant with a 6-fold increased affinity over the starting variant.
To test whether multiple mutations obtained firom hGH-phage selections would produce cumulatively favorable effects on hGHbp (hGH-binding protein; the extracellular domain of the hGH receptor) Minding, we combined mutations found in the three tightest-binding variants of hGH from the helix-1 library (Example IX:
F10AIM14WIH18DlH21N, F10H/M14G/H18N/H21N, and F10FIM14S/H18F/H21L) with those found in the three tightest binding variants found in the helix-4b library (Example X:
R167N/D171SlT175II179T, R167EID171S/T175/I179, and R167N/D171NIT17511179T).
hGH-phagemid double-stranded DNA (dsDNA) from each of the one-helix variants was isolated and digested with the restriction enzymes EcoRl and BstXl. The large fragment from each helix-4b variant was then isolated and ligated with the small fragment from each helix-1 variant to yield the r~ew two-helix variants shown in Table XIII. All of these variants also contained the mutations E174S/F176Y
obtained in earlier hGH-phage binding selections (see Example X for details).
Although additivity prindples appear to hold for a number of comt~ir~ations of mutations, some comt~inations (e.g. E174S with F176Y) are Dearly non-additive (see examples VIII and X). In order to identify with certainty the tightest binding variant with, for example, 4 mutations in helix-1 ~ 4 mutations in helix-4, one would ideally mutate all 8 residues at once and then sort the pool for the globally tightest binding variant.
However, such a pool would consist of 1.1 x 1012 DNA sequences (utilizing NNS
colon degeneracy) encoding 2.6 x 1010 different polypeptides. Obtaining a random phagemid library large enough to assure representation of all variants (pefiaps 1013 transfonnants) is not practical using current transformation technology.
We have addressed this difficulty first by utilizing successive rounds of mutagenesis, taking the tightest Minding variant from one library, then mutating other residues to further improve binding (Example X).
In a second method, we have utilized the principle of additivity to comt~ir~e the best mutations from two independently sorted libraries to create multiple mutants with improved binding (described above). Here, we further searched through the possible comtHnations of mutations at positions 10,14,18, 21,167,171,175, and 179 in hGH, by creating comt~inatorial libraries of random or partially-random mutants. We constructed three different comt~inatorial libraries of hGH-phagemids, using the pooled phagemids from the helix 1 library (independently sorted for 0, 2, or 4 cycles; Example IX) and the pool from the helix-4b library (independently sorted for 0, 2, or 4 cyGes; Example X) and sorted the combined variant pool for hGHbp binding. Since some amount of sequence diversity exists in each of these pools, the resulting combinatorial library can explore more sequence combinations than what we might constmct manually (e.g. Table XIII).
WO 92/09690 2 ~ ~ ~ ~ ~ ~ PCT/US91 /09133 hGH-phagemid double-stranded DNA (dsDNA) from each of the one-helix library pools (selected for 0, 2, or 4 rounds) was isolated and digested with the restriction enzymes Ac~cl and BstXl. The large fragment from each helix-1 variant pool was then isolated and ligated with the small fragment from each helix-4b vaunt pool to yield the three combinatorial libraries pH0707A (unselecbed helix 1 and helix 4b pools, as described in examples IX
5 and X), pH0707B (twice-selected helix-1 pool with twice-selected helix-4b pool), and pH0707C (4-times selected helix-1 pool with 4-times selected helix-4b pool). Duplicate ligations were also set up with less DNA and designated as pH0707D, pH0707E, and pH0707F, corresponding to the 0-,2-, and 4-round starting libraries respectively. All of these variant pools also contained the mutations E174SIF176Y obtained in earlier hGH-phage binding selectans (see Example X for details).
The ligation products pH0707A-F were processed and electro-transformed into XL1-Blue cells as described (Example VIII). Based on colony-forming units (CFU), the number of transformants obtained from each pool was as follows: 2.4x106 from pH0707A, 1.8x106 from pH0707B, 1.6x106 from pH0707C, 8x105 from pH0707D, 3x105 from pH0707E, and 4x105 from pH0707F. hGH-phagemid particles were prepared and selected for hGHbp-binding over 2 to 7 cycles as described in Example VIII.
In additan to sorting phagemid libraries for tight-binding protein variants, as measured by equilibrium binding affinity, it is of interest to sort for variants which are altered in either the on-rate (kon) or the off-rate (koff) of binding to a receptor or other molecule. From thermodynamics, these rates are related to the equilibrium dissodation constant, I(d = (koff/kon). We envision that certain variants of a particular protein have similar Kd's for binding while having very different kon's and ko ff's.
Conversely, d~anges in Kd trom one variant to another may be due to effects on kon, effects on ko ff, or both. The pharmacological properties of a protein may be dependent on Minding affinity or on kon or koff, depending on the detailed mechanism of action. Here, we sought to identify hGH variants with higher on-rates to investigate the effects of d~anges in kon. We envision that the selection could alternatively be weighted toward koff by increasing the binding time and increasing the wash time andlor concentration with cognate ligand (hGH).
From time-course analysis of wild-type hGH-phagemid Minding to immobilized hGHbp, it appears that, of tt~e total hGH-phagemid particles that can be eluted in the final pH 2 wash (see Example VIII for the complete Minding and elution protocol), less than 10% are bound affer 1 minute of incubation, while greater than 90% are bound affer 15 minutes of incubation.
For 'rapid-binding selection,' phagemid particles from the pH0707B pool (twice-selected for helices 1 and 4 independently) were incubated with immobilized hGHbp for only 1 minute, then washed six times with 1 mL of tHnding buffer; the hGH-wash step was omitted; and the remaining hGH-phagemid particles were eluted with a pH2 (0.2M glydne in binding buffer) wash. Enrichment of hGH-phagemid particles over non~iisplaying particles indicated that even with a short binding period and no cognate-ligand (hGH) d~allenge, hGH-phagemid binding selection sorts tight-Minding variants out of a randomized pool.
5, ~~ ~~3~
The binding constants for some of these mutants of hGH to hGHbp was determined by expressing the tree hormone variants in the non-suppressor E. colt strain 1609 or 3488, purifying the protein, and assaying by competitive displacement of labelled wt-hGH from hGHbp (see Example VIII) in a radio-immunoprecipitation assay.
In Table XIII -A below, all the variants have glutamate174 replaced by serine174 and pher~lalaninel7g replaced by tyrosine176 (E174S and F1176Y) plus the additional substitutions as indicated at hGH amino aad positions 10, 14,18, 21,167,171,175 and 179.
Table XIII-A
hGH
variaMS
from addpfon of helix-1 and helix~b mutaUons li H
li wild-type residue:F1Q e ~il,$ Jj21HlCtZ e I1Z~ 1128 ri x x nt X14 ~1Z1 Va H G N N N S T T
a In Table XIV below, hGH variants were selected from combinatorial libraries by the phagemid binding selection process. All hGH variants in Table XIV contain two background mutatans (E174SlF176Y). hGH-phagemid pools from the libraries pH0707A (Part A), pH0707B and pH0707E (Part B), or pH0707C (Part C) were sorted for 2 to 7 cycles for Minding to hGHbp. The number P indicates the fractional occurrence of each variant type among the set of Bones sequerxed from each pool.
WO PC'1'/US91/09133 ~~~~~~3 52 Table XIV
hGH variants from hOrllnor~e-pt~agemld binc9np selection Of comhlnalortal I~r~aries.
Hela Helix wild-type ~Q ~ J~$ ~ g~j residue:
Variant Part 4 cycles:
A :
0.60 H0714A.1 H G N N N S T N
0.40 H0714A.4 A N D A N N T N
' Part 8:
2 Cycles:
0.13 H0712B.1 F S F G H S T T
0.13 H0712B.2 H Q T S A D N S
0.13 H0712B.4 H G N N N A T T
0.13 H0712B.5 F S F L S D T T
0.13 H0712B.6 A S T N R D T I
0.13 H0712B.7 Q Y N N H S T T
0.13 H0712B.8 W G S S R D T I
0.13 H0712E.1 F L S S K N T V
0.13 H0712E.2 W N N S H S T T
0.13 H0712E.3 A N A S N S T T
0.13 H0712E.4 P S D N R D T I
0.13 H07t2E.5 H G N N N N T S
0.13 H0712E.6 F S T G R D T I
0.13 H0712E.7 M T S N Q S T T
0.13 H0712E.8 F S F L T S T S
4 cycles:
0.17 H0714B.1 A W D N R D T I
0.17 H0714B.2 A W D N H S T N
0.17 H0714B.3 M Q M N N S T T
0.17 H0714B.4 H Y D H R D T T
0.17 H0714B.5 L N S H R D T I
0.17 H0714B.6 L N S H T S T T
7cydes:
0.57 H0717B.1 A W D N N A T T
0.14 H0717B.2 F S T G R D T I
0.14 H07178.6 A W D N R D T I
0.14 H0717B.7 I Q E H N S T T
0.50 H0717E.1 F S L A N S T V
Part C:
4 cycles:
0.67 H0714C.2 F S F L K D T T
' = also contained the mutations L15R, K168R.
In Table XV below, hGH variants were selected from combinatorial libraries by the phagemid binding selection process. All hGH variants in Table XV contain two background mutations (E174SIF176Y). The number 5 0 P is the fractional occurrence of a given variant among all Gones sequenced after 4 cycles of rapid-binding selection.
' Table XV
hGH variants from RAPID hGHbp bindklp selection d en hGH-phagernld comblnatortel IIbrJry Helot Heluc wild-type residue: ~Q ~g ~$ )~ g~ p~j~
Yaf~t 0.14 H07BF4.2 W G S S R D T I
0.57 H078F4.3 M A D N N S T T
0.14 H07BF4.6 A W D N S S V T $
0.14 H078F4.7 H Q T S R D T I
$ = also contained the mutatan Y176F (wild-type hGH also contains F176j.
In table XVI below, binding constants were measured by competitive displacement of 1251-labelled hormone H06508D or labelled hGH using hGHbp (1-238) and either Mab5 or Mab263.
The variant H06508D
appears hind more than 30-fold tighter than wild-type hGH.
~~~~~J~ 5a Table XVI
EquIAbrium blndinp oo~ants of selected hGH wadaMs.
hGH Kd,(vanantl Kd,(variantl Variant Kd(H0650BD) Kd(hGH) Kd (per hGH 3 2 -1- 340 t H0650BD -1- 0.031 tOt 3 H0650BF 1.5 0.045 15 t 5 H07148.6 3.4 0.099 34 t 19 H0712B.7 7.4 0.22 74 t 30 H0712E.2 16 0.48 60 f 70 EXAMPLE XIII
Selectirve enrichment of hGH-phage contalnlng a pr~tesse substrate sequence versus ron-substrate phage As described in Example I, the plasmid pS0132 contains the gene for hGH fused to the residue Pro198 of the gene III protein with the insertion of an extra glyane residue. This plasmid may be used to produce hGH-phage particles in which the hGH~ene III fusion product is displayed monovalently on the phage surtace (Example IV). The fusion protein comprises the entire hGH protein fused to the carboxy terminal domain of gene III via a flexible linker sequerxe.
3 0 To investigate the feasibility of using phage display technology to select favourable substrate sequences for a given proteolytic enzyme, a genetically engineered variant of subtilisin BPN' was used. (Carter, P.
et al., Proteins: Structure, function and genetics 6:240-248 (1989)). This variant (hereafter referred to as A64SAL subtilisin) contains the following mutations: Ser24Cys, His64Ata, GIu156Ser, GIy169A1a and Tyr217Leu. Since this enzyme lacks the essential catalytic residue His64, its substrate speaficity is greatly restricted so that certain histidine-containing substrates are preferentially hyrdrolysed (Carter et al., Science 237:394-399 (1987)).
The sequence of the linker region in pS0132 was mutated to create a substrate sequence for A64SAL
subtilisin, using the oligonucleotide 5'-TTC-GGG-CCC-TTC-GCT-GCT-CAC-TAT-ACG-CGT-CAG-TCG-ACT-GAC-CTG-CCT-3'. This resulted in the introduction of the protein sequence Phe-Gly-Pro-Phe-Ala-Ala-5 His-Tyr-Thr-Arg-Gln-Ser-Thr-Asp in the linker region between hGH and the carboxy terminal domain of gene III, where the first Phe residue in the above sequence is Phe191 of hGH. The sequence Ala-Ala-His-Tyr-Thr-Agr-Gln is known to be a good substrate for A64SAL subtilisin (Carter et al (1989), supra). The resulting plasmid was designated pS0640.
Phagemid particles derived from pS0132 and pS0640 were constructed as described in Example I. In initial experiments, a 10W aliquot of each phage pool was separately mixed with 30p1 of oxirane beads (prepared as described in Example II) in 100W of buffer comprising 20mM Tris-HCI pH 8.6 and 2.5M NaCI. The binding and washing steps were performed as described in example VII. The beads were then resuspended in 400p1 of the same buffer, with or without 50nM of A64SAL subtilisin. Following incubation for 10 minutes, the supernatants were collected and the phage titres (cfu) measured. Table XVII shows that approximately 10 times more substrate-containing phagemid particles (pS0640) were eluted in the presence of enzyme than in the absence of enzyme, or than in the case of the non-substrate phagemids (pS0132) in the presence or absence of enzyme. Increasing the enzyme, phagemid or bead concentrations did not improve this ratio.
In an attempt to decrease the non-speafic elution of immobilised phagemids, a tight-bir>ding variant of hGH was introduced in place of the wild-type hGH gene in pS0132 and pS0640. The hGH variant used was as described in example XI (pH0650bd) and contains the mutations PhelOAla, Mett4Trp, Hisl8Asp, His2lAsn, Arg167Asn, Asp171Ser, GIu174Ser, Phe176Tyr and IIe179Thr. This resulted in the construction of two new phagemids: pDM0390 (containing tight-Minding hGH and no substrate sequence) and pDM0411 (containing tight-binding hGH and the substrate sequence Ala-Ala-His-Tyr-Thr-Agr-Gln). The binding washing and elution protocol was also changed as follows:
(i) Binding: COSTAR 12-well tissue culture plates were coated for 16 hours with 0.5mUwell 2ug/ml hGHbp in sodium carbonate buffer pH 10Ø The plates were then incubated with lmllwell of blocking buffer (phosphate buffered saline (PBS) containing 0.1%w/v bovine serum albumen) for 2 hours and washed in an assay buffer containing lOmM Tris-HCI pH 7.5, 1 mM EDTA and 1 OOmM NaCI. Phagemids were again prepared as described in Example I: the phage pool was diluted 1:4 in the above assay buffer and 0.5m1 of phage incubated per well for 2 hours.
WO 92/09690 ~. ~ PCT/US91/09133 ~~~~~~3 (ii) Washing: The plates were washed thoroughly with PBS + 0.05% Tween 20 and incubated for 30 minuted with 1 ml of this wash buffer. This washing step was repeated three times.
(~i) Eution: The plates were incubated for 10 minutes in an elution buffer consisting of 20mM Tris-HCI pH 8.6 + 100mM NaCI, then the phage were eluted with 0.5m1 of the above buffer with or without 500nM of A64SAL subtilisin.
Table XVII shows that there was a dramatic increase in the ratio of specifically eluted substrate-phagemid particles compared to the method previously described for pS0640 and pS0132. ft is likely that this is due to the fact that the tight-binding hGH
mutant has a significantly slower off-rate for binding to hGH binding protein compared to wild-type hGH.
Table XVII
Specific elution of substrate-phagemlds by A64SAL subtllisln Colony forming units (cfu) were estimated by plating out 10.1 of 10-fold dilutions of phage on l0pl spots of XL-1 blue cells, on LB agar plates containing 50pg/ml carbenicillinl (i) wld-type hGH gene: binding to hGHbp-oxirane beads pS0640 (substrate) 9x106cfu/l0wl 1.5x106cfu/l0pl pS0132 (non-substrate) 6x105cfu/l0pl 3x105cfu/l0pl (ii) pH0650bd mutant hGH gene: Minding to hGHbp-coated plates pDM0411 (substrate) 1.7x105cfu/l0pl 2x103cfu/l0pl pDM0390 (non-substrate) 2x103cfu/l0pl tx103cfu/l0pl Example XIV
Identification of preferred substrates for A64SAL subtllisln using selective enrichment of a library of substrate sequences.
We sought to employ the selective enrichment procedure described in Example XIII to identify good substrate sequences from a library of random substrate sequences.
We designed a vector suitable for introduction of randomised substrate cassettes. and subsequent expression of a library of substrate sequences. The starting point was the vector pS0643, described in Example VIII. Site-directed mutagenesis was carried out using the oligonucleotide 5'-AGC-TGT-GGC-TTC-GGG-CCC-GCC~CC-GCG-TCG-ACT-GGC-GGT-GGC-TCT-3', which introduces ~ (GGGCCC) and ~,[ (GTCGAC) restriction sites between hGH
and Gene III. This new construct was designated pDM0253 (The actual sequence of pDM0253 is 5'-AGC-TGT-GGC-TTC-GGG-CCC-GCC-ACC-GCG-TCG-ACT-GGC-GGT-GGC-TCT-3', where WO 92/09690 ~~ ~? ~ ~, PCT/US91 /09133 57 ~~~5~~1~5 the underlined base substitution is due to a spurious error in the mutagenic oligonucleotide).
In addition, the tight-binding hGH variant described in example was introduced by exchanging a fragment from pDM0411 (example XII I) The resurting library vector was designated pDM0454.
To introduce a library cassette, pDM0454 was digested with Apal folbwed by Sall, then precipitated with 13% PEG 8000+ lOmM MgCl2, washed twice in 70% ethanol and resuspended This effidently precipitates the vector but leaves the small Apa-Sal fragment in solution (Paithankar, K. R. and Prasad, K. S. N., Nuceic Acids Research 19:1346). The product was run on a 1% agarose gel and the Apal-Sall digested vector excised, purified using a Bandprep kit (Pharmacia) and resuspended for Ngation with the mutagenic cassette.
The cassette to be inserted contained a DNA sequence similar to that in the linker region of pS0640 and pDM0411, but with the colons for the histidine and tyrosine residues in the substrate sequence replaced by randomised colons. We chose to substitute NNS
(N=G/A/T/C; S=G/C) at each of the randomised positions as described in example VIII. The oligonucleotides used in the mutagenic cassettes were: 5'-C-TTC-GCT-GCT-NNS-NNS-ACC-CGG-CAA-3' (coding strand) and 5'-T-CGA-TTG-CCG-GGT-SNN-SNN-AGC-AGC-GAA-GGG-CC-3' (non-coding strand). This cassette also destroys the Sall site, so that digestion with Sall may be used to reduce the vector background. The oGgonucleotides were not phosphorylated before insertion into the Apa-Sal cassette site, as it was feared that subsequent oGgomerisation of a small population of the cassettes may lead to spurious results with multiple cassette inserts. Following annealing and ligation, the reaction products were phenol:chloroform extracted, ethanol precipitated and resuspended in water.
Initially, no digestion with Sall to reduce the background vector was pertormed.
Approximately 200ng was electroporated into XL-1 blue cells and a phagemid library was prepared as described in example VIII.
Selection of hlyhly cleavable substrates from the snb_c_trate Ilbrarv The selection procedure used was identical to that described for pDM0411 and pDM0390 in example XIII. After each round of selection, the eluted phage were propagated by transducing a fresh culture of Xl_-1 blue cells and propagating a new phagemid library as described for hGH-phage in example VIII. The progress of the selection procedure was monitored by measuring eluted phage titres and by sequencing individual clones after each round of selection.
Table A shows the successive phage titres for elution in the presence and absence of enzyme after 1, 2 and 3 rounds of selection.
2~~~~33 58 Clearly, the ratio of specifically eluted phage: non-specifically eluted phage (ie phage eluted with enzyme:phage eluted without enzyme) increases dramatically from round 1 to round 3, suggesting that the population of good substrates is increasing with each round of selection.
Sequencing of 10 isolates from the starting library showed them all to consist of the wild-type pDM0464 sequence. This is attributed to the fact that after digestion with Apal, the Sall site is very close to the end of the DNA fragment, thus leading to bw effiaency of digestion. Nevertheless, there are only 400 possible sequences in the library, so this population should still be well represented.
Tables B1 and B2 shows the sequences of isolates obtained after round 2 and round 3 of selection. Affer 2 rounds of selection, there is clearly a high incidence of histidine residues. This is exactly what is expelled: as described in example XIII, A64SAl_ subtilisin requires a histidine residue in the substrate as it employs a substrate-assisted catalytic mechanism. After 3 rounds of selection, each of the 10 Gones sequenced has a histidine in the randomised cassette. Note, however, that 2 of the sequences are of pDM0411, which was not present in the starting library and is therefore a contaminant.
WO 92/09690 ~ ~ ~ ~ ~ ~ ~ PCT/US91 Table A
Titration of Initialphape pools and phage from 3 rounds of eluted selective enrichment Colony forming units (cfu) were estimated by plating out 101,1.1 of 10-fold dilutions of phage on 101~J spots of XL-1 aining 501ig/ml carbenicillin blue cells, on LB
agar plates cont Starting library: 3x1012 cfu/ml LIBRARY: +500nM A64SAL : 4x103 cfu/101~I
no enzyme : 3x103 cfu/101~1 pDM0411: +500nM A64SAL : 2x106 cfu/10111 (control) no enzyme : 8x103 cfu/101~I
Round 1 library: 7x1012 cfu/ml LIBRARY: +500nM A64SAL : 3x104 cfu/l0wl no enzyme : 6x103 cfu/101~1 pDM0411: +500nM A64SAL : 3x106 cfu110111 (control) no enzyme : 1.6x104 cfu/101~I
Round 2 library: 7x1011 cfu/ml LIBRARY: +500nM A64SAL : 1x105 cfu/10111 no enzyme : <103 cfu/l0wl pDM0411: +500nM A64SAL : 5x106 cfu/101~I
(control) no enzyme : 3x104 cfu/101~1 so Table B1 Sequences of eluted phage after 2 rounds of selective enrichment.
All protein sequences should be of the form AA"TRO, where ' represents a randomised colon. In the table below, the randomised colons and amino acids are underlined and in bold.
After round 2:
A A $ Y T R Q
... GCT GCT~~~ ACC CGGCAA ... 2 TAC
A A $ j~j T R Q
... GCT GCT ACC CGGCAA ... 1 A A ji $ T R Q
... GCT GCT~ ACC CGGCAA ... 1 A A Z $ T R Q
... GCT GCT~ ACC CGGCAA ... 1 A A $ T R Q
.. . GCT~~ CGG CAA... 1 #
GCT
A A ~ $ T R Q
... GCT GCT~~~ ACC CGGCAA 1 ##
CAC
... wild-type 3 pDM0454 # - spurious deletion of 1 colon within the cassette ## - ambiguous sequence ~~~~~~3 Table B2 Seauences of eluted ohaqe after 3 rounds of selective enrichment.
All protein represents sequences should a be of the form AA"TRQ, where ' randomised colon.the table below, the randomised In colons and amino acids are underlined and in bold.
After round 3:
nce o-of Seau occurrences A A $ ~ T R Q
... GCT GCT ACG CGT CAG ... 2 ~
A A ji $ T R Q
... GCT GCT CAC ACC CGG CAA ... 2 CTC
2O A A Q $ T R Q
... GCT GCT ACC CGG CAA ... 1 ~
A A T $ T R Q
... GCT GCT ACC CGG CAA ... 1 A A $ ,~, R Q
... GCT GCT TCC CGG CAA ... 1 CAC
A A $ $ T R Q
... GCT GCT ACC CGG CAA 1 ~
A A $ $ R Q
... GCT GCT TTC CGG CAA ... 1 CAC
A A $ T R Q
... GCT GCT CGG CAA ... 1 # - contaminating sequence from pDM0411 ## - contains "illegal" colon CAT - T should the not appear in the 3rd position of a colon.
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Garrard, Lisa J.
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Bass, Steven Greene, Ronald Lowman, Henry B.
Wells, James A.
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~a~5~~~
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~~'~~~~3 (2) INFORMATION FOR SEQ ID NO:11:
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Gly Ser Cys Gly Phe Glu Ser Gly Gly Gly Ser Gly (2) INFORMATION FOR SEQ ID N0:14:
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n N ~ eJ Ey 'L
(2) INFORMATION FOR SEQ ID N0:16:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 30 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:16:
(2) INFORMATION FOR SEQ ID N0:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:17:
(2) INFORMATION FOR SEQ ID N0:18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:18:
(2) INFORMATION FOR SEQ ID N0:19:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 25 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:
(2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
(2) INFORMATION
FOR
SEQ
ID
N0:21:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 66 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:21:
TCGAGGCTCN NSGACAACGC GNNSCTGCGT GCTNNSCGTC TTNNSCAGCT
(2) INFORMATION
FOR
SEQ
ID
N0:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 58 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:22:
GTGTCAAAGG CCAGCTGSNN AAGACGSNNA GCACGCAGSN NCGCGTTGTC
(2) INFORMATION
FOR
SEQ
ID
N0:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 65 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION: SEQ ID N0:23:
GTTACTCTAC TGCTTCNNSA AGGACATGNN SAAGGTCAGC NNSTACCTGC
(2) INFORMATION
FOR
SEQ
ID
N0:24:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 64 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear WO 92/09690 PC1'/US91/09133 209~~~~
(xi) SEQUENCE DESCRIPTION:SEQ ID
N0:24:
(2) INFORMATION FOR SEQ
ID N0:25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2178 bases (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION:SEQ ID
N0:25:
~J ~
~~~
~
(2) INFORMATION
FOR
SEQ
ID
N0:26:
(i) SEQUENCE
CHARACTERISTICS:
(A) acids LENGTH:
amino 20 (B) amino TYPE: acid (D) linear TOPOLOGY:
(xi) SEQID
SEQUENCE N0:26:
DESCRIPTION:
25 Met LysLysAsn IleAlaPhe LeuLeuAla SerMetPhe ValPhe Ser IleAlaThr AsnAlaTyr AlaAspIle GlnMetThr GlnSer Pro SerSerLeu SerAlaSer ValGlyAsp ArgValThr IleThr Cys ArgAlaSer GlnAspVal AsnThrAla ValAlaTrp TyrGln Gln LysProGly LysAlaPro LysLeuLeu IleTyrSer AlaSer 40 Phe LeuTyrSer GlyValPro SerArgPhe SerGlySer ArgSer Gly ThrAspPhe ThrLeuThr IleSerSer LeuGlnPro GluAsp Phe AlaThrTyr TyrCysGln GlnHisTyr ThrThrPro ProThr Phe GlyGlnGly ThrLysVal GluIleLys ArgThrVal AlaAla Pro SerValPhe IlePhePro ProSerAsp GluGlnLeu LysSer 55 Gly ThrAlaSer ValValCys LeuLeuAsn AsnPheTyr ProArg Glu AlaLysVal GlnTrpLya ValAspAsn AlaLeuGln SerGly Asn SerGlnGlu SerValThr GluGlnAsp SerLyaAsp SerThr Tyr SerLeuSer SerThrLeu ThrLeuSer LysAlaAsp TyrGlu Lys HisLysVal TyrAlaCys GluValThr HisGlnGly LeuSer Ser Pro Val Thr Lys Ser Aen ArgGly GluCys Phe (2) INFORMATION FOR SEQ
ID N0:27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 461 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (xi) SEQUENCE DESCRIPTION:SEQ ID
N0:27:
Met Lys Lys Asn Ile Ala Leu LeuAla SerMet PheVal Phe Phe Ser Ile Ala Thr Asn Ala Ala GluVal GlnLeu ValGlu Tyr Ser Gly Gly Gly Leu Val Gln Gly GlySer LeuArg LeuSer Pro Cys Ala Ala Ser Gly Phe Asn Lys AspThr TyrIle HisTrp Ile Val Arg Gln Ala Pro Gly Lys Leu GluTrp ValAla ArgIle Gly Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lye Gly Arg Phe Thr IleSerAla AspThrSer LysAsn ThrAlaTyr LeuGln Met Asn SerLeuArg AlaGluAsp ThrAla ValTyrTyr CysSer Arg Trp GlyGlyAsp GlyPheTyr AlaMet AspTyrTrp GlyGln Gly Thr LeuValThr ValSerSer AlaSer ThrLysGly ProSer Val Phe ProLeuAla ProSerSer LysSer ThrSerGly GlyThr Ala Ala LeuGlyCys LeuValLys AspTyr PheProGlu ProVal Thr Val SerTrpAsn SerGlyAla LeuThr SerGlyVal HisThr Phe Pro AlaValLeu GlnSerSer GlyLeu TyrSerLeu SerSer Val Val ThrValPro SerSerSer LeuGly ThrGlnThr TyrIle Cys Asn ValAsnHis LysProSer AsnThr LysValAsp LysLys Val Glu ProLysSer CysAspLys ThrHis ThrGlyPro PheVal Cys Glu TyrGlnGly GlnSerSer AspLeu ProGlnPro ProVal Asn Ala GlyGlyGly SerGlyGly GlySer GlyGlyGly SerGlu Gly GlyGly SerGlu GlyGlyGly SerGluGly GlyGlySer Glu Gly GlyGly SerGly GlyGlySer GlySerGly AspPheAsp Tyr Glu LysMet AlaAsn AlaAsnLys GlyAlaMet ThrGluAsn Ala Asp GluAsn AlaLeu GlnSerAsp AlaLysGly LysLeuAap Ser Val AlaThr AspTyr GlyAlaAla IleAspGly PheIleGly Asp Val SerGly LeuAla AsnGlyAsn GlyAlaThr GlyAspPhe Ala Gly SerAsn SerGln MetAlaGln ValGlyAsp GlyAspAsn Ser Pro LeuMet AsnAsn PheArgGln TyrLeuPro SerLeuPro Gln Ser ValGlu CyaArg ProPheVal PheSerAla GlyLysPro Tyr Glu PheSer IleAsp CysAspLys IleAsnLeu PheArgGly Val Phe AlaPhe LeuLeu TyrValAla ThrPheMet TyrValPhe Ser Thr PheAla AsnIle LeuArgAsn LysGluSer
Claims
1. A human growth hormone variant, (a) wherein hGH amino acids 172, 174, 176 and 178 respectively are as a group sequentially selected from the group consisting of: (1)R,S,F,R; (2)R,A,Y,R; (3)K,T,Y,K; (4)R,S,Y,R; (5)K,A,Y,R;
(6)R,F,F,R; (7)K,Q,Y,R; (8)R,T,Y,H; (9)Q,R,Y,R; (10)K,K,Y,K;
(11)R,S,F,S and (12)K,S,N,R;
(b) wherein hCH amino acids 10, 14, 18, and 21 respectively are as a group sequentially selected from the group consisting of: (1) H,G,N,N; (2) A,W,D,N; {3) F,S,F,L; (4) Y,T,V,N and (5) I,N,I,N;
(c) wherein hGH amino acids 174 is serine and 176 is tyrosine and hGH amino acids 167, 171, 175 and 179 respectively are as a group sequentially selected from the group consisting of: (1) N,S.T.T; (2) E.S,T,I; (3) K,S,T,L; (4) N,N,T,T; (5) R,D,I,I and (6) N,S,T,Q;
(d) wherein hGH amino acid glutamate174 is replaced by serine174 and phenylalanine176 is replaced by tyrosine176 and one ar more of the eight naturally occurring hGH amino acids F10, M14, H18, H21, R167, D171, T175 and I179 are replaced by another natural amino acid to provide a variant capable of binding to human growth hormone receptor;
(e) wherein in the hGH variant of (d) the eight naturally occurring hGH amino acids F10, M14, H18, W21, R167, D171, T175 and I179 respectively are as a group replaced with a corresponding amino acid sequentially selected from the group consisting of:
(1) H, G, N, N, N, S, T, T; (2) H, G, N, N, E, S, T, I;
(3) H. G, N, N, N, N, T, T; (4) A, W, D, N, N, S, T, T;
(5) A, W, D, N, E, S, T, I; (6) A, W, D, N, N, N, T, T;
(7) F, S, F, L, N, S, T, T; (8) F, S, F, L, E, S, T, I;
(9) F, S, F, L, N, N, T, T; (10) H, G, N, N, N, S, T, N;
(11) A, N, D, A, N, N, T, N; (12) F, S, F, G, H, S, T, T;
(13) H, Q, T, S, A, D, N, S. (14) H, G, N, N, N, A, T, T;
(15) F, S, F, L, S, D, T, T; (18) A, S, T, N, R, D, T, I;
(17) Q, Y, N, N, H, S, T, T; (18) W, G, S, S, R, D, T, I;
(19) F, L, S, S, K, N, T, V; (20) W, N, N, S, H, S, T, T;
(21) A, N, A, S, N, S, T, T; (22) P, S, D, N, R, D, T, I;
(23) H, G, N, N, N, N, T, S; (24) F, S, T, G, R, D, T, I;
(25) M, T, S, N, Q, S, T, T; (26) F, S, F, L, T, S, T, S;
(27) A, W, D, N, R, D, T, I; (28) A, W, D, N, H, S, T, N;
(29) M, Q, M, N, N, S, T, T; (30) H, Y, D, H, R, D, T, T;
(31) L, N, S, H, R, D, T, I; (32) L, N, S, H, T, S, T, T;
(33) A, W, D, N, N, A, T, T; (34) F, S, T, G, R, D, T, I;
(35) A, W, D, N, R, D, T, I; (36) I, Q, E, H, N, S, T, T;
(37) F, S, L, A, N, S, T, V; (38) F, S, F, L, K, D, T, T;
(39) M, A, D, N, N, S, T, T; (40) A, W, D, N, S, S, V, T; and (41) H, Q, Y, S, R, D, T, I;
(f) wherein in the hGH variant of (e) the human growth hormone variant further contains leucine15 replaced by arginine15 and lysine168 replaced by arginine168;
(g) wherein in the hGH variant of (e) the human growth hormone variant further contains phenylalanine176.
(6)R,F,F,R; (7)K,Q,Y,R; (8)R,T,Y,H; (9)Q,R,Y,R; (10)K,K,Y,K;
(11)R,S,F,S and (12)K,S,N,R;
(b) wherein hCH amino acids 10, 14, 18, and 21 respectively are as a group sequentially selected from the group consisting of: (1) H,G,N,N; (2) A,W,D,N; {3) F,S,F,L; (4) Y,T,V,N and (5) I,N,I,N;
(c) wherein hGH amino acids 174 is serine and 176 is tyrosine and hGH amino acids 167, 171, 175 and 179 respectively are as a group sequentially selected from the group consisting of: (1) N,S.T.T; (2) E.S,T,I; (3) K,S,T,L; (4) N,N,T,T; (5) R,D,I,I and (6) N,S,T,Q;
(d) wherein hGH amino acid glutamate174 is replaced by serine174 and phenylalanine176 is replaced by tyrosine176 and one ar more of the eight naturally occurring hGH amino acids F10, M14, H18, H21, R167, D171, T175 and I179 are replaced by another natural amino acid to provide a variant capable of binding to human growth hormone receptor;
(e) wherein in the hGH variant of (d) the eight naturally occurring hGH amino acids F10, M14, H18, W21, R167, D171, T175 and I179 respectively are as a group replaced with a corresponding amino acid sequentially selected from the group consisting of:
(1) H, G, N, N, N, S, T, T; (2) H, G, N, N, E, S, T, I;
(3) H. G, N, N, N, N, T, T; (4) A, W, D, N, N, S, T, T;
(5) A, W, D, N, E, S, T, I; (6) A, W, D, N, N, N, T, T;
(7) F, S, F, L, N, S, T, T; (8) F, S, F, L, E, S, T, I;
(9) F, S, F, L, N, N, T, T; (10) H, G, N, N, N, S, T, N;
(11) A, N, D, A, N, N, T, N; (12) F, S, F, G, H, S, T, T;
(13) H, Q, T, S, A, D, N, S. (14) H, G, N, N, N, A, T, T;
(15) F, S, F, L, S, D, T, T; (18) A, S, T, N, R, D, T, I;
(17) Q, Y, N, N, H, S, T, T; (18) W, G, S, S, R, D, T, I;
(19) F, L, S, S, K, N, T, V; (20) W, N, N, S, H, S, T, T;
(21) A, N, A, S, N, S, T, T; (22) P, S, D, N, R, D, T, I;
(23) H, G, N, N, N, N, T, S; (24) F, S, T, G, R, D, T, I;
(25) M, T, S, N, Q, S, T, T; (26) F, S, F, L, T, S, T, S;
(27) A, W, D, N, R, D, T, I; (28) A, W, D, N, H, S, T, N;
(29) M, Q, M, N, N, S, T, T; (30) H, Y, D, H, R, D, T, T;
(31) L, N, S, H, R, D, T, I; (32) L, N, S, H, T, S, T, T;
(33) A, W, D, N, N, A, T, T; (34) F, S, T, G, R, D, T, I;
(35) A, W, D, N, R, D, T, I; (36) I, Q, E, H, N, S, T, T;
(37) F, S, L, A, N, S, T, V; (38) F, S, F, L, K, D, T, T;
(39) M, A, D, N, N, S, T, T; (40) A, W, D, N, S, S, V, T; and (41) H, Q, Y, S, R, D, T, I;
(f) wherein in the hGH variant of (e) the human growth hormone variant further contains leucine15 replaced by arginine15 and lysine168 replaced by arginine168;
(g) wherein in the hGH variant of (e) the human growth hormone variant further contains phenylalanine176.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002405246A CA2405246A1 (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with alterred binding properties |
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US62166790A | 1990-12-03 | 1990-12-03 | |
US07/621,667 | 1990-12-03 | ||
US68340091A | 1991-04-10 | 1991-04-10 | |
US07/683,400 | 1991-04-10 | ||
US71530091A | 1991-06-14 | 1991-06-14 | |
US07/715,300 | 1991-06-14 | ||
US74361491A | 1991-08-08 | 1991-08-08 | |
US07/743,614 | 1991-08-08 | ||
PCT/US1991/009133 WO1992009690A2 (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with altered binding properties |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002405246A Division CA2405246A1 (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with alterred binding properties |
Publications (2)
Publication Number | Publication Date |
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CA2095633A1 CA2095633A1 (en) | 1992-06-04 |
CA2095633C true CA2095633C (en) | 2003-02-04 |
Family
ID=27505166
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
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CA002405246A Abandoned CA2405246A1 (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with alterred binding properties |
CA002095633A Expired - Lifetime CA2095633C (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with altered binding properties |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
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CA002405246A Abandoned CA2405246A1 (en) | 1990-12-03 | 1991-12-03 | Enrichment method for variant proteins with alterred binding properties |
Country Status (9)
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US (7) | US5750373A (en) |
EP (1) | EP0564531B1 (en) |
AT (1) | ATE164395T1 (en) |
CA (2) | CA2405246A1 (en) |
DE (1) | DE69129154T2 (en) |
DK (1) | DK0564531T3 (en) |
ES (1) | ES2113940T3 (en) |
GR (1) | GR3026468T3 (en) |
WO (1) | WO1992009690A2 (en) |
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