CA2079802A1 - Walk-through mutagenesis - Google Patents

Walk-through mutagenesis

Info

Publication number
CA2079802A1
CA2079802A1 CA002079802A CA2079802A CA2079802A1 CA 2079802 A1 CA2079802 A1 CA 2079802A1 CA 002079802 A CA002079802 A CA 002079802A CA 2079802 A CA2079802 A CA 2079802A CA 2079802 A1 CA2079802 A1 CA 2079802A1
Authority
CA
Canada
Prior art keywords
antibodies
libraries
amino acids
mutagenesis
function
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002079802A
Other languages
French (fr)
Other versions
CA2079802C (en
Inventor
Roberto Crea
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2079802A1 publication Critical patent/CA2079802A1/en
Application granted granted Critical
Publication of CA2079802C publication Critical patent/CA2079802C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0002—Antibodies with enzymatic activity, e.g. abzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Abstract

A method of mutagenesis by which a predetermined amino acid is introduced into each and every position of a selected set of positions in a preselected region (or several different regions) of a protein to produce library of mutants. The method is based on the premise that certain amino acids play crucial role in the structure and function of proteins. Libraries can be generated which contain a high proportion of the desired mutants and are of reasonable size for screening. These libraries can be used to study the role of specific amino acids in protein structure and function and to develop new or improved proteins and polypep-tides such as enzymes, antibodies, single chain antibodies and catalytic antibodies.
CA002079802A 1990-04-05 1991-04-05 Walk-through mutagenesis Expired - Lifetime CA2079802C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50531490A 1990-04-05 1990-04-05
US505,314 1990-04-05
PCT/US1991/002362 WO1991015581A1 (en) 1990-04-05 1991-04-05 Walk-through mutagenesis

Publications (2)

Publication Number Publication Date
CA2079802A1 true CA2079802A1 (en) 1991-10-06
CA2079802C CA2079802C (en) 2001-09-18

Family

ID=24009833

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002079802A Expired - Lifetime CA2079802C (en) 1990-04-05 1991-04-05 Walk-through mutagenesis

Country Status (8)

Country Link
US (3) US5798208A (en)
EP (1) EP0527809B1 (en)
JP (2) JP4251406B2 (en)
AT (1) ATE126535T1 (en)
CA (1) CA2079802C (en)
DE (1) DE69112207T2 (en)
ES (1) ES2078518T3 (en)
WO (1) WO1991015581A1 (en)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030194807A1 (en) * 1992-11-02 2003-10-16 Roberto Crea Walk-through mutagenesis
DE69331336T2 (en) * 1993-04-09 2002-08-14 Catalytic Antibodies Inc SELECTION OF GENES CATALYTIC ANTIBODIES
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5683987A (en) * 1994-07-12 1997-11-04 The Board Of Regents Of The University Of Nebraska Therapeutic oligonucleotides targeting the human MDR1 and MRP genes
US6063562A (en) * 1994-09-16 2000-05-16 Sepracor, Inc. In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst
US5602021A (en) * 1994-12-29 1997-02-11 Catalytic Antibodies, Inc. Method for generating proteolytic enzymes specific against a selected peptide sequence
US6537776B1 (en) * 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US20030219752A1 (en) * 1995-12-07 2003-11-27 Diversa Corporation Novel antigen binding molecules for therapeutic, diagnostic, prophylactic, enzymatic, industrial, and agricultural applications, and methods for generating and screening thereof
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
BR9713955A (en) * 1996-12-20 2000-05-23 Unilever Nv Enzymatic bleaching composition, and process for bleaching stains present in fabrics.
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE19731990A1 (en) * 1997-07-25 1999-01-28 Studiengesellschaft Kohle Mbh Process for the production and identification of new hydrolases with improved properties
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide
EP1051484A1 (en) * 1998-01-30 2000-11-15 Sepracor, Inc. Gene regulator fusion proteins and methods of using the same for determining resistance of a protein to a drug targeted there against
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
EP1073710A4 (en) * 1999-02-04 2007-12-19 Verenium Corp Non-stochastic generation of genetic vaccines and enzymes
JP3399518B2 (en) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション Semiconductor structure and method of manufacturing the same
AU3879300A (en) * 1999-03-09 2000-09-28 Diversa Corporation End selection in directed evolution
US6251604B1 (en) * 1999-08-13 2001-06-26 Genopsys, Inc. Random mutagenesis and amplification of nucleic acid
US7115712B1 (en) 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
WO2001073000A2 (en) * 2000-03-24 2001-10-04 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
US8288322B2 (en) * 2000-04-17 2012-10-16 Dyax Corp. Methods of constructing libraries comprising displayed and/or expressed members of a diverse family of peptides, polypeptides or proteins and the novel libraries
CA2406236C (en) * 2000-04-17 2013-02-19 Dyax Corp. Novel methods of constructing libraries of genetic packages that collectively display the members of a diverse family of peptides, polypeptides or proteins
AU2001275530A1 (en) * 2000-06-08 2001-12-17 Board Of Regents, The University Of Texas System Inhibitors of c-reactive protein induced inflammation
FR2813314B1 (en) * 2000-08-25 2004-05-07 Biomethodes MASSIVE DIRECTED MUTAGENESIS PROCESS
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
PT2316940E (en) 2000-12-18 2013-10-16 Dyax Corp Focused libraries of genetic packages
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
JP2004532038A (en) * 2001-05-17 2004-10-21 ディヴァーサ コーポレイション Application of novel antigen-binding molecules to therapeutic, diagnostic, prophylactic, enzymatic, industrial and agricultural fields, and methods for producing and screening novel antigen-binding molecules therefor
SE0102327D0 (en) 2001-06-28 2001-06-28 Active Biotech Ab A novel engineered superantigen for human therapy
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US20040096826A1 (en) * 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
US20030224404A1 (en) * 2002-02-25 2003-12-04 Manuel Vega High throughput directed evolution of nucleic acids by rational mutagenesis
US20030171543A1 (en) * 2002-03-05 2003-09-11 Bott Richard R. High throughput mutagenesis screening method
US20030199068A1 (en) * 2002-03-05 2003-10-23 Bott Richard R. High throughput mutagenesis screening method
JP4493347B2 (en) * 2002-04-17 2010-06-30 ロベルト クレア Universal library of immunoglobulins
EP1495142B1 (en) * 2002-04-17 2007-09-26 Bioren, Inc. "doping" in walk-through mutagenesis
ES2285118T5 (en) * 2002-05-17 2012-09-21 Alligator Bioscience Ab A METHOD FOR IN VITRO MOLECULAR DEVELOPMENT OF A PROTEIC FUNCTION.
BR0313281A (en) 2002-08-06 2007-07-24 Verdia Inc ap1 amine oxidase variants
US20040067532A1 (en) * 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
JP4796299B2 (en) 2002-08-12 2011-10-19 ジェンネレックス インコーポレイティッド Methods and compositions for poxviruses and cancer
US7785799B2 (en) * 2002-08-16 2010-08-31 The Board Of Regents Of The University Of Texas System Compositions and methods related to flavivirus envelope protein domain III antigens
ATE466085T1 (en) * 2002-09-09 2010-05-15 Hanall Pharmaceutical Co Ltd PROTEASE-RESISTANT MODIFIED INTERFERON ALPHA POLYPEPTIDES
JP2007524380A (en) 2003-04-02 2007-08-30 ボード オブ リージェンツ ザ ユニバーティー オブ テキサス システム Bik mutant with antitumor effect
EP2322629A3 (en) 2003-04-29 2011-11-02 Pioneer Hi-Bred International Inc. Novel glyphosate-n-acetyltransferase (GAT) genes
BRPI0412007A (en) * 2003-06-27 2006-08-15 Bioren Inc look-through mutagenesis
DK1664343T3 (en) 2003-09-09 2014-08-11 Integrigen Inc METHODS AND COMPOSITIONS FOR GENERATING CHIMELY HUMAN ANTIBODY GENES
BRPI0513155B1 (en) * 2004-07-06 2021-07-20 Bioren, Inc. METHOD OF DISTINGUISHING ONE OR MORE FUNCTIONAL AMINO ACID RESIDUES FROM NON-FUNCTIONAL AMINO ACID RESIDUES IN A DEFINED REGION WITHIN A POLYPEPTID
WO2006014498A2 (en) 2004-07-06 2006-02-09 Bioren, Inc. Universal antibody libraries
BRPI0511448A (en) * 2004-07-06 2007-12-26 Bioren Inc high affinity anti-tnf-alpha antibodies, generation method and sequence library
WO2006114700A2 (en) * 2005-04-26 2006-11-02 Bioren, Inc. Method of producing human igg antibodies with enhanced effector functions
US20070026012A1 (en) 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
KR20090004839A (en) * 2005-09-07 2009-01-12 제네렉스, 인코포레이티드 Systemic treatment of metastatic and/or systemically-disseminated cancers using gm-csf-expressing poxviruses
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
US8716195B2 (en) 2005-11-14 2014-05-06 Bioren, Inc. Antibody ultrahumanization by predicted mature CDR blasting and cohort library generation and screening
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
EP2522678A1 (en) 2006-05-15 2012-11-14 Sea Lane Biotechnologies, LLC Neutralizing antibodies to influenza viruses
US20080108513A1 (en) * 2006-06-01 2008-05-08 Northwestern University Cellular Arrays
JP2010500876A (en) 2006-08-18 2010-01-14 アブリンクス エン．ヴェー． Amino acid sequence directed against IL-6R and polypeptides comprising the same for the treatment of diseases and disorders associated with IL-6 mediated signaling
CA2921048C (en) 2006-09-15 2018-06-05 Ottawa Hospital Research Institute Oncolytic rhabdovirus
WO2008130437A2 (en) * 2006-10-20 2008-10-30 Arizona Board Of Regents For And On Behalf Of Arizona State University Modified cyanobacteria
EP2118281A2 (en) 2007-02-12 2009-11-18 Codexis, Inc. Structure-activity relationships
US20100106585A1 (en) * 2007-04-17 2010-04-29 American Express Travel Related Services Company, Inc. System and method for evaluating positive behavior and offering incentives based upon limited use identifier transactions
AU2008287426B2 (en) * 2007-08-10 2014-06-26 Protelica, Inc. Universal fibronectin type III binding-domain libraries
US8680019B2 (en) * 2007-08-10 2014-03-25 Protelica, Inc. Universal fibronectin Type III binding-domain libraries
US8470966B2 (en) 2007-08-10 2013-06-25 Protelica, Inc. Universal fibronectin type III binding-domain libraries
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3753947A1 (en) 2007-09-14 2020-12-23 Adimab, LLC Rationally designed, synthetic antibody libraries and uses therefor
AU2008328785A1 (en) 2007-11-27 2009-06-04 Ablynx N.V. Method for obtaining polypeptide constructs comprising two or more single domain antibodies
US9873957B2 (en) * 2008-03-13 2018-01-23 Dyax Corp. Libraries of genetic packages comprising novel HC CDR3 designs
AU2009240481B2 (en) * 2008-04-24 2015-07-30 Takeda Pharmaceutical Company Limited Libraries of genetic packages comprising novel HC CDR1, CDR2, and CDR3 and novel LC CDR1, CDR2, and CDR3 designs
EP2383292A1 (en) * 2008-05-02 2011-11-02 Novartis AG Improved fibronectin-based binding molecules and uses thereof
EP3629022A1 (en) 2008-07-25 2020-04-01 Richard W. Wagner Protein screening methods
JP5230397B2 (en) * 2008-12-18 2013-07-10 独立行政法人科学技術振興機構 Method for determining antigen-binding site of anti-transmembrane protein antibody
WO2010111707A1 (en) 2009-03-27 2010-09-30 Sapphire Energy, Inc. Variant isoprenoid producing enzymes and uses thereof
WO2010115998A2 (en) 2009-04-10 2010-10-14 Ablynx Nv Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders
EP2417162A2 (en) 2009-04-10 2012-02-15 Ablynx N.V. Improved amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of il-6r related diseases and disorders
CA2764398A1 (en) 2009-06-05 2010-12-09 Erik Depla Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
KR101759888B1 (en) 2009-09-14 2017-07-20 신라젠(주) Oncolytic vaccinia virus combination cancer therapy
US20110082054A1 (en) * 2009-09-14 2011-04-07 Dyax Corp. Libraries of genetic packages comprising novel hc cdr3 designs
US9139825B2 (en) 2009-10-30 2015-09-22 Novartis Ag Universal fibronectin type III bottom-side binding domain libraries
WO2011051327A2 (en) 2009-10-30 2011-05-05 Novartis Ag Small antibody-like single chain proteins
WO2011051466A1 (en) 2009-11-02 2011-05-05 Novartis Ag Anti-idiotypic fibronectin-based binding molecules and uses thereof
WO2011064382A1 (en) 2009-11-30 2011-06-03 Ablynx N.V. Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections
EP2510088B1 (en) 2009-12-10 2016-10-05 Ottawa Hospital Research Institute Oncolytic rhabdovirus
WO2011092233A1 (en) 2010-01-29 2011-08-04 Novartis Ag Yeast mating to produce high-affinity combinations of fibronectin-based binders
US9354228B2 (en) 2010-07-16 2016-05-31 Adimab, Llc Antibody libraries
CN107903321A (en) 2010-07-30 2018-04-13 诺华有限公司 Fibronectin cradle molecule and Qi Ku
CA2805803A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity
US9919047B2 (en) 2011-01-04 2018-03-20 Sillajen, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
EP2718427B1 (en) 2011-06-08 2017-01-11 Children's Hospital of Eastern Ontario Research Institute Inc. Compositions for glioblastoma treatment
US10138302B2 (en) 2011-09-23 2018-11-27 Ablynx N.V. Methods for treating rheumatoid arthritis by administering interleukin-6 receptor antibodies
KR102434073B1 (en) 2011-10-11 2022-08-18 비엘라 바이오, 인크. Cd40l-specific tn3-derived scaffolds and methods of use thereof
EP2820043B1 (en) 2012-03-02 2020-01-15 Ablynx N.V. Biparatopic pseudomonas aeruginosa pcrv binding single variable domain antibodies
CN104411823A (en) 2012-05-04 2015-03-11 纳幕尔杜邦公司 Compositions and methods comprising sequences having meganuclease activity
WO2014087010A1 (en) 2012-12-07 2014-06-12 Ablynx N.V. IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE
CA2901501C (en) 2013-02-21 2023-03-07 Children's Hospital Of Eastern Ontario Research Institute Inc. Vaccine composition
BR112015023272A2 (en) 2013-03-14 2017-07-18 Pioneer Hi Bred Int plant cell, plant, plant explant, transgenic seed, method for producing a plant cell having a heterologous polynucleotide encoding a polypeptide having dicamba decarboxylase activity, method for controlling weeds in a field containing a crop and method for controlling weeds in a field containing a culture
EP2970935A1 (en) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
EP3209327A1 (en) 2014-10-21 2017-08-30 Ablynx N.V. Treatment of il-6r related diseases
EP3842451A1 (en) 2015-03-12 2021-06-30 MedImmune, LLC Method of purifying albumin-fusion proteins
JP6962819B2 (en) 2015-04-10 2021-11-05 アディマブ， エルエルシー Method for Purifying Heterodimer Multispecific Antibody from Parent Homodimer Antibody Species
JP6867302B2 (en) 2015-05-21 2021-04-28 フル スペクトラム ジェネティクス， インコーポレイテッド How to improve protein characteristics
CN110291402B (en) 2016-06-27 2023-09-01 朱诺治疗学股份有限公司 Method for identifying peptide epitopes, molecules binding such epitopes and related uses
MA45491A (en) 2016-06-27 2019-05-01 Juno Therapeutics Inc CMH-E RESTRICTED EPITOPES, BINDING MOLECULES AND RELATED METHODS AND USES
WO2018237323A1 (en) 2017-06-23 2018-12-27 The Board Of Trustees Of The Leland Stanford Junior University Pde5a destabilizing domains
EP3723788A4 (en) 2017-12-15 2022-04-13 Aleta Biotherapeutics Inc. Cd19 variants

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6130597A (en) * 1984-07-20 1986-02-12 Nippon Zeon Co Ltd Device for synthesizing polynucleotide
ZA893284B (en) * 1988-05-04 1990-03-28 Igen Inc Peptide analogs and their use as haptens to elicit catalytic antibodies
AU633003B2 (en) * 1988-05-11 1993-01-21 Polygen Holding Corporation Polypeptide compounds having growth hormone releasing activity
DE68927933T2 (en) * 1988-09-02 1997-08-14 Dyax Corp PRODUCTION AND SELECTION OF RECOMBINANT PROTEINS WITH DIFFERENT BINDING POINTS
US6780613B1 (en) * 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5096815A (en) * 1989-01-06 1992-03-17 Protein Engineering Corporation Generation and selection of novel dna-binding proteins and polypeptides
CA2009996A1 (en) * 1989-02-17 1990-08-17 Kathleen S. Cook Process for making genes encoding random polymers of amino acids
FI87028C (en) * 1989-12-22 1992-11-10 Nokia Mobile Phones Ltd METHOD OF RESULT OF EFFECTIVE PROCEDURE WITHOUT SPREADING OF EFFECTIVE EFFECTS AND COVERING OF METHODS
US5264563A (en) * 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons

Also Published As

Publication number Publication date
US5830650A (en) 1998-11-03
EP0527809B1 (en) 1995-08-16
WO1991015581A1 (en) 1991-10-17
ATE126535T1 (en) 1995-09-15
AU653152B2 (en) 1994-09-22
US5798208A (en) 1998-08-25
DE69112207D1 (en) 1995-09-21
JP4275922B2 (en) 2009-06-10
ES2078518T3 (en) 1995-12-16
JP2003135084A (en) 2003-05-13
EP0527809A1 (en) 1993-02-24
AU7741891A (en) 1991-10-30
CA2079802C (en) 2001-09-18
DE69112207T2 (en) 1996-03-28
US6649340B1 (en) 2003-11-18
JPH05506580A (en) 1993-09-30
JP4251406B2 (en) 2009-04-08

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