CA2074135A1 - Procede d'immobilisation d'un fragment d'acide nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation - Google Patents

Procede d'immobilisation d'un fragment d'acide nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation

Info

Publication number
CA2074135A1
CA2074135A1 CA002074135A CA2074135A CA2074135A1 CA 2074135 A1 CA2074135 A1 CA 2074135A1 CA 002074135 A CA002074135 A CA 002074135A CA 2074135 A CA2074135 A CA 2074135A CA 2074135 A1 CA2074135 A1 CA 2074135A1
Authority
CA
Canada
Prior art keywords
solid support
support
nucleic acid
acid fragment
immobilizing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002074135A
Other languages
English (en)
Other versions
CA2074135C (fr
Inventor
Philippe Cros
Patrice Andre Allibert
Bernard Fabien Mandrand
Pascal Thierry Dalbon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2074135A1 publication Critical patent/CA2074135A1/fr
Application granted granted Critical
Publication of CA2074135C publication Critical patent/CA2074135C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

Procédé d'immobilisation par fixation passive, sur un support solide, d'un fragment d'acide nucléique comportant moins de 100 nucléotides, dans lequel on dépose sur ledit support ledit fragment sous la forme d'un dérivé résultant du couplage covalent dudit fragment avec un ligand ayant une masse moléculaire inférieure à 5000 et comprenant au moins un groupement fonctionnel polaire, ledit dérivé n'étant pas susceptible de réagir sur ledit support avec formation d'une liaison covalente dans les conditions dudit dépôt, étant entendu que lorsque ledit ligand est un nucléotide ou oligonucléotide, il comprend au moins un nucléotide modifié de façon à introduire ledit groupement fonctionnel polaire, et support solide ainsi obtenu. Ce support peut être utilisé notamment comme sonde de capture pour la détection d'une cible nucléotidique.
CA002074135A 1991-07-17 1992-07-17 Procede d'immobilisation d'un fragment d'acide nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation Expired - Fee Related CA2074135C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9109057 1991-07-17
FR9109057A FR2679255B1 (fr) 1991-07-17 1991-07-17 Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.

Publications (2)

Publication Number Publication Date
CA2074135A1 true CA2074135A1 (fr) 1993-01-18
CA2074135C CA2074135C (fr) 2001-04-17

Family

ID=9415243

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002074135A Expired - Fee Related CA2074135C (fr) 1991-07-17 1992-07-17 Procede d'immobilisation d'un fragment d'acide nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation

Country Status (9)

Country Link
US (1) US5510084A (fr)
EP (1) EP0524864B1 (fr)
JP (1) JP2797047B2 (fr)
AU (1) AU659176B2 (fr)
CA (1) CA2074135C (fr)
DE (1) DE69225646T2 (fr)
ES (1) ES2118120T3 (fr)
FI (1) FI102619B1 (fr)
FR (1) FR2679255B1 (fr)

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FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
FR2707010B1 (fr) * 1993-06-25 1995-09-29 Bio Merieux
US5656427A (en) * 1994-08-29 1997-08-12 Gen-Probe Incorporated Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
CA2207629A1 (fr) * 1994-12-22 1996-06-27 Abbott Laboratories Procedes d'immobilisation d'oligonucleotides sur des materiaux de support et procedes d'utilisation des oligonucleotides fixes auxdits supports
FR2733755B1 (fr) * 1995-05-03 1997-07-11 Bio Merieux Fragment nucleotidique de l'arn ribosomique 16s de corynebacteries, sondes et amorces derivees, reactif et procede de detection
FR2758884B1 (fr) * 1997-01-30 1999-04-02 Bio Merieux Procede pour isoler, notamment detecter ou quantifier un analyte dans un milieu
US5932711A (en) * 1997-03-05 1999-08-03 Mosaic Technologies, Inc. Nucleic acid-containing polymerizable complex
US6692912B1 (en) 1997-03-05 2004-02-17 Matrix Technologies Corporation Nucleic acid-containing polymerizable complex
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US6429007B1 (en) 1997-05-02 2002-08-06 BIOMéRIEUX, INC. Nucleic acid amplification reaction station for disposable test devices
US5989499A (en) * 1997-05-02 1999-11-23 Biomerieux, Inc. Dual chamber disposable reaction vessel for amplification reactions
US5786182A (en) * 1997-05-02 1998-07-28 Biomerieux Vitek, Inc. Dual chamber disposable reaction vessel for amplification reactions, reaction processing station therefor, and methods of use
US6410275B1 (en) 1997-05-02 2002-06-25 Biomerieux, Inc. Disposable test devices for performing nucleic acid amplification reactions
US6558901B1 (en) 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
US5858653A (en) * 1997-09-30 1999-01-12 Surmodics, Inc. Reagent and method for attaching target molecules to a surface
US6762019B2 (en) * 1997-09-30 2004-07-13 Surmodics, Inc. Epoxide polymer surfaces
US6465178B2 (en) * 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (fr) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection et isolement d'un polymorphisme de mononucleotide
CA2353596A1 (fr) * 1998-12-04 2000-06-15 Mosaic Technologies, Inc. Methode d'immobilisation d'oligonucleotides
US20050153926A1 (en) * 1998-12-04 2005-07-14 Adams Christopher P. Method for the immobilization of oligonucleotides
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
GB0016836D0 (en) * 2000-07-07 2000-08-30 Lee Helen Improved dipstick assays (1)
US6706479B2 (en) * 2000-10-05 2004-03-16 Virginia Tech Intellectual Properties, Inc. Bio-chip, photoluminescent methods for identifying biological material, and apparatuses for use with such methods and bio-chips
CA2473376A1 (fr) 2002-01-16 2003-07-31 Dynal Biotech Asa Methode permettant d'isoler des acides nucleiques et des proteines contenus dans un echantillon unique
US20040067495A1 (en) * 2002-10-08 2004-04-08 Ray Gustav Allen Polynucleic acid and polyamino acid binding substrate
US7309593B2 (en) * 2003-10-01 2007-12-18 Surmodics, Inc. Attachment of molecules to surfaces
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
EP1623996A1 (fr) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Procédé amélioré de selection d'une protéine desirée d'une bibliothéque.
JP4899067B2 (ja) * 2006-02-23 2012-03-21 国立大学法人北海道大学 質量分析用生体関連分子のエステル化法及び得られたエステル化誘導体の質量分析方法
EP2140001A2 (fr) * 2007-04-25 2010-01-06 3M Innovative Properties Company Procédés d'amplification d'acides nucléiques
RU2011142154A (ru) * 2009-03-19 2013-04-27 Сепс Фарма Н.В. Производные фосфлокуназола, их синтез и применение в препаратах длительного действия
EP4341270A1 (fr) * 2021-05-21 2024-03-27 Olix US, Inc. Réactifs de phosphorylation chimique, préparation et leurs utilisations

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4379843A (en) * 1981-01-26 1983-04-12 Peter Cashion Immobilization of polynucleotides and polypeptides with tritylated polysaccharides
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US4948882A (en) * 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
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US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
ATE88762T1 (de) * 1986-08-11 1993-05-15 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
EP0383803B1 (fr) * 1987-10-28 2000-05-03 Howard Florey Institute Of Experimental Physiology And Medicine Conjugues d'oligonucleotide-polyamide
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
EP0405913B1 (fr) * 1989-06-30 1998-12-23 Chiron Corporation Sonde d'acide nucléique hydrophobe
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
EP0504321B1 (fr) * 1989-12-04 1997-05-07 Microprobe Corporation Capture amelioree d'acide nucleique cible faisant appel a des oligonucleotides attaches de fa on covalente a des polymeres
CA2032203C (fr) * 1989-12-29 2009-05-19 Christine L. Brakel Essai de capture par amplification
FR2663040B1 (fr) * 1990-06-11 1995-09-15 Bio Merieux Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
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US5138045A (en) * 1990-07-27 1992-08-11 Isis Pharmaceuticals Polyamine conjugated oligonucleotides

Also Published As

Publication number Publication date
AU2037992A (en) 1993-01-21
ES2118120T3 (es) 1998-09-16
FI102619B (fi) 1999-01-15
DE69225646D1 (de) 1998-07-02
FR2679255B1 (fr) 1993-10-22
FI923260A (fi) 1993-01-18
EP0524864A1 (fr) 1993-01-27
EP0524864B1 (fr) 1998-05-27
FI102619B1 (fi) 1999-01-15
FR2679255A1 (fr) 1993-01-22
JP2797047B2 (ja) 1998-09-17
FI923260A0 (fi) 1992-07-16
AU659176B2 (en) 1995-05-11
US5510084A (en) 1996-04-23
JPH05271272A (ja) 1993-10-19
CA2074135C (fr) 2001-04-17
DE69225646T2 (de) 1998-12-24

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