CA2067203A1 - Method of cryopreserving musculoskeletal tissues - Google Patents

Method of cryopreserving musculoskeletal tissues

Info

Publication number
CA2067203A1
CA2067203A1 CA2067203A CA2067203A CA2067203A1 CA 2067203 A1 CA2067203 A1 CA 2067203A1 CA 2067203 A CA2067203 A CA 2067203A CA 2067203 A CA2067203 A CA 2067203A CA 2067203 A1 CA2067203 A1 CA 2067203A1
Authority
CA
Canada
Prior art keywords
cryopreserving
cell
glycosaminoglycan
tissue
musculoskeletal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2067203A
Other languages
French (fr)
Other versions
CA2067203C (en
Inventor
Kelvin G. M. Brockbank
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Artivion Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2067203A1 publication Critical patent/CA2067203A1/en
Application granted granted Critical
Publication of CA2067203C publication Critical patent/CA2067203C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents

Abstract

Disclosed herein is a method for cryopreserving musculoskeletal tissues, such as ligaments, tendons and cartilage, by placing such tissue in contact with a composition containing a cryopreserving agent comprising a cell-penetrating organic solute, which is preferably dimethylsulfoxide, and a glycosaminoglycan, which is preferably chondroitin sulphate, in an amount sufficient to cryopreserve the musculoskeletal tissue. The addition of a glycosaminoglycan to a cryopreserving agent comprising a cell-penetrating organic solute permits a broad range of cooling rates to be employed, rather than the very narrow ranges which are employed using a cryopreserving agent comprising a cell-penetrating organic solvent without the glycosaminoglycan. Also disclosed are a freezing schedule designed to maximize retention of tissue cell viability and biomechanical properties during and after the freezing process, and a thawing schedule which maximizes cell viability.
CA002067203A 1989-11-03 1990-11-02 Method of cryopreserving musculoskeletal tissues Expired - Fee Related CA2067203C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US431,153 1989-11-03
US07/431,153 US5131850A (en) 1989-11-03 1989-11-03 Method for cryopreserving musculoskeletal tissues
PCT/US1990/006221 WO1991006213A1 (en) 1989-11-03 1990-11-02 Method of cryopreserving musculoskeletal tissues

Publications (2)

Publication Number Publication Date
CA2067203A1 true CA2067203A1 (en) 1991-05-04
CA2067203C CA2067203C (en) 2000-01-11

Family

ID=23710715

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002067203A Expired - Fee Related CA2067203C (en) 1989-11-03 1990-11-02 Method of cryopreserving musculoskeletal tissues

Country Status (5)

Country Link
US (1) US5131850A (en)
EP (1) EP0500788A4 (en)
AU (1) AU7740091A (en)
CA (1) CA2067203C (en)
WO (1) WO1991006213A1 (en)

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Also Published As

Publication number Publication date
EP0500788A1 (en) 1992-09-02
EP0500788A4 (en) 1992-11-19
WO1991006213A1 (en) 1991-05-16
CA2067203C (en) 2000-01-11
AU7740091A (en) 1991-05-31
US5131850A (en) 1992-07-21

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