CA2061636C - Chimeric gene for the transformation of plants - Google Patents

Chimeric gene for the transformation of plants Download PDF

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Publication number
CA2061636C
CA2061636C CA002061636A CA2061636A CA2061636C CA 2061636 C CA2061636 C CA 2061636C CA 002061636 A CA002061636 A CA 002061636A CA 2061636 A CA2061636 A CA 2061636A CA 2061636 C CA2061636 C CA 2061636C
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dna sequence
gene
rubisco
sequence
plastid
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CA2061636A1 (en
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Michel Lebrun
Bernard Leroux
Alain Sailland
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Bayer SAS
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Rhone Poulenc Agrochimie SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Abstract

Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS is disclosed. It comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastic-localised enzyme. The invention also provides for the production of glyphosate-tolerant plants.

Description

~j CHIMERIt~ GENE FOR THE TRANSF(7RMATION OF PLANTS
The present invention relates to novel transit peptide DNA
sequences,to novel chimeric genes and to their use in plants for confernng to them an increased tolerance to herbicides in general especially to those of the phosphonomethylglycine farruly. It also relates to the plant cells transformed by means of these genes, to the transformed plants regenerated from these cells as well as to the plants derived from crossbreedings using these transformed plants.
Glyphosate, sulfosate or fosametine are broad-spectrum systemic herbicides of the phosphonomethyl-glycine family. They act essentially as competitive inhibitors of 5-(enolpyruvyl)shilcimate-3- phosphate synthase (EC
2.5.1.19) or EPSPS in relation to PEP (phosphoenolpyruvate). After their application to the plant, they are translocated inside the plant where they accumulate in the rapidly growing parts, in particular the caulinary and root apexes, causing the deterioration and even the destruction of sensitive plants.
Plastidial EPSPS, the main target of these products, is an enzyme of the aromatic amino acid biosynthesis pathway which is encoded by one or more nuclear genes and synthesised in the form of a cytoplasmic precursor and then imported into the plastids where it accumulates in its natural form.
'The tolerance of plants to glyphosate and to products of the family is obtained by the stable introduction inside their genome of an EPSPS gene of plant or bacterial origin mutant or nonmutant with respect to the characteristics of the inhibition of the product of this gene by glyphosate. Given the mode of action of glyphosate and the degree of tolerance to glyphosate of the product of the genes used, it is useful to be able to express the product of iranslatian of this gene so as to permit its substantial accumulation in plastids.

It is known, for example from American Patent 4,535,060, to confer to a plant a tolerance to a herbicide of the abovementioned type, in particular N-(phosphonomethyl)glycine or glyphosate, by introducing into the plant genome a gene encoding an EPSPS carrying at least one mutation making this enzyme more resistant to its competitive inhibitor (glyphosate), after localisation of the enzyme in the plastidial compartment. However, these techniques need to be improved in order to achieve greater reliability in the use of these plants under agronomic conditions.
In the present description, "plant" is understood as meaning any differentiated multicellular organism capable of I>hotosynthesis and "plant cell" any cell derived from a plant and capable of forming undifferentiated tissues such as calluses or differentiated tissues such as embr3~os or plant sections, plants or seeds.
The subject of thf; present invention is the production of transformed plants having an increased tolerance to herbicides in general and especially to those of the phosphonomethylglycine family by regenerating cells transformed by means of novel chimeric genes comprising a gene ivor tolerance to these herbicides. The invention also relates to these novel chimeric genes, to the novel transit peptides which they contain as well as to the plants containing them which are made more tolerant by an accumulation of the muta:nt enzyme, in its mature form, in the plants.
More particularly,, the subject of the invention is a chimeric gene for conferring to plants an increased tolerance to a herbicide whose target is EPSPS, comprising, in the direction of transcription, a promoter region, a transit peptide region, a sequence of a gene encoding a glyphosate tolerance enzyme and an untranslated polyadenylation signal region in 3', wherein the transit peptide region comprises, in the direction of transcription, a transit peptide of a plant gene encoding a plastid-localised enzyme, a partial. sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastid-localised enzyme.
The invention also relates to any DNA sequence of the transit peptide region defined above.
The transit peptides which c;an be used in the transit peptide region may be known per se and may be of plant origin, for example, derived from maize, sunflower, peas, tobacco or the like. The first and the second transit peptides may be identical, analogous or different. '.Chey may in addition each comprise one or more transit peptide units. A sequence derived from the small subunit (SSU) of the ribulose 1,5-diphosphate carboxyla;~e oxygenase (RuBisCO) gene is preferably used The partial sequence of the N-terminal mature part is derived from a plant gene encoding a plastid-localised enzyme, such as for example a maize, sunflower or pea gene or the like, it being possible for the original plant species to be identical, analogous or different from that from which the first and second transit peptides are derived respectively. Furthermore, the partial sequence of the mature part may comprise a varying number of amino acids, generally from 10 to 40, preferably from 18 to 33. A sequence derived from the SSU of the ribulose 1,5-diphosphate carboxylase oxygenase (F;uBisCO) ,gene is preferably used.
Construction of the entire transit region may be carried out in a manner known per se, in particular. by fusion or any other suitable means. The role of this characteristic region is to enable the: release of a mature, native protein with a maximum efficiency.
The coding sequence for herbicide tolerance which may be used in the chimeric gene according to the invention encodes a mutant EPSPS having a degree of glyphosate tolerance. This sequence, obtained in particular by mutation of the EPSPS
gene, may be of bacterial origin, for example derived from Salmonella typhymurium (and called in the text which follows ".AroA gene"'), or of plant origin, for example from petunia or from tomatoes. This sequence may comprise one or more mutations, for example the Pro 101 to Ser mutation or alternatively the Gly 96 to .Ala mutations.
The promoter region of the chimeric gene according to the invention may consist advantageously of at least one promoter or a fragment thereof of a gene which is expressed naturally in plants, that is to say promoters of viral origin such as that of 35S RNA of the cauliflower mosaic virus (CaMV35S) or of plant origin such as the small subunit of the ribulose 1,5-diphosphate carboxylase: (RuBisC'O) gene of a crop such as maize or sunflower.
l0 The untranslated polyadenylation signal region in 3' of the chimeric gene according to the invention may be of any origin, for example bacterial, such as the nopaline synthase gene, or of plant origin, such as the small subunit of the maize or sunflower RuBisCO.
The chimeric gene according to the invention may comprise, in addition to the above essential parts, an untnanslated inoermediate region (linker) between the promoter region and the coding sequence whiich may be of any origin, bacterial, viral or plant.
EXAMPLE 1: CONSTRUCTION CIF A CHIMERIC GENE:
The construction of the: chimeric gene according to the invention is carried out using the following elements.:
1 ) "Double CaMV" promoter (that is to say part of which has been duplicated):
The CaMV35S promoter was isolated by Odell et al. (1985, Nature 313, 810-812).
A clone, pJ0 5-2, containing about 850 by upstream of the site of initiation of transcription was cut with EcoRI-HindIII, the ends ofthis isolated fr,~gment were made blunt using Klenow polymerase and the fragment iraerted at the HincII site ol~the vector pUC 19 (Yannish-Perron et al., 1985, Gene 33(1 ),103-119). This promoter was dige: ted with CIaI, the ends filled using Klenow polymerase Icd:md then redigested with HindIII. A HindIII- EcoRV fragment, isolated from the same initial promoter, was introduced between these two sites. The promoter thus obtained possesses a double amplification region upstream of the regulatory elements of the CaMV35S
promoter. It was introduced in the form of a HindIII-EcoRI fragment into the vector pRPA-BL
150 A apha 2, described in French Patent Application 88/04130 (laid open on March 23, 1988), cut with HindIII and EcoRI.
2) Transfer region: the two transit peptides as well as the mature protein elements used are derived from the cloned cDNA of the small subunit of the gene of maize RuBisCO whose gene has been described by Lebrun et al. (1987, Nucleic Acids Research 15(10), 4360), and from the cloned cDNA ofthe small subunit ofthe gene of sunflower RuBisCO, isolated by Waksman et al. (1987, Nucleic Acids Research 15(17), 7181). More specifically, the transit region, called optimised transit peptide, comprises, in the direction of translation:
- a transit peptide of the small subunit of sunflower RuBisCO, - an N-terminal sequence of 22 amino acids of the mature part of the small subunit of maize RuBisCO, - a transit peptide of the small subunit of maize RuBisCO.
The construct using this optimised transfer peptide is called pRPA-BL 410.
Other similar sequences may be used which contain sequences of 10 to 40 and preferably 18 and 33 amino acids respectively.
In order to provide a comparative element, another construction was carried out using a first transit peptide and the same mature sequence part but without a second transit peptide, according to the prior art (pRPA-BL 294).
3) Structuralgene: it is derived from the mutant gene at the position (Pro 101 to Ser) of EPSPS of Salmonella typhymurium isolated by Stalker et al. (1985, Journal of Biological Chemistry 260(8), 4724-4728). The pMG34-2 clone (provided by Calgene) was ICd:md linearised with XbaI and then treated with Vigna radiata nuclease. After recutting with SmaI, the two blunt ends were ligated. The clone obtained possesses an NcoI site in the initiator ATG as well as a 17-by SaII site downstream of the stop codon. This clone was called pRPA-BL 104.
4) Polyadenylation si n~gion: the fragment is derived from the nopaline synthase gene of pTi37 (Bevan et al., 1983, Nucleic Acids Research 11(2), 525-539). This site is contained in a 260-by MboI fragment (Fraley et al., 1983, Proc. Nat. Acad.
Sci. USA 80, 4803-4807; Patent Application PCT 84/02913 (16.01.1984, Fraley R.T.) laid open on January 16, 1984) which was treated with Klenow polymerase and cloned in the SmaI site of M13 mp 18 in order to introduce the BamHI and EcoRI sites at the 5' and 3' ends respectively.
After cutting with BamHI and treating with Vigna radiata nuclease followed by cutting with EcoRI and treating with Klenow polymerase, the resulting fragment was introduced in the vector p-BL 20 (cf. French Patent Application 88/04130 (23.03.1988, Lebrun M., FR)), cut by XbaI and BamHI and treated with Klenow polymerase. After recutting with SaII and SstI, a fragment of about 0.4 kbp containing the 3' nos sequence on the side of the SaII site and the right end on the T-DNA side of the SstI site is obtained.
The assembly of the various elements was carried out in the following manner:
"Transit peptide of the SSU of the maize RuBisCO/AroA gene" fusion:
The transit peptide of the S SU of the maize RuBisCO gene is derived from a by EcoRI-SphI fragment obtained from the cDNA corresponding to the SSU gene of the maize RuBisCO gene, described by Lebrun et al. (1987, Nucleic Acids Research 15(10), 4360), possessing an NcoI site spanning the initiation codon for translation and an SphI site corresponding to the cleavage site of the transit peptide.
Translational fusion is obtained between the maize transit peptide and the bacterial EPSPS gene by treating the SphI end with bacteriophage T4 polymerase and by ligating it with Icd:md the Klenow polymerise-treated NcoI end of the AroA gene from pRPA-BL 104, recut with EcoRI.
Transit peptide of the S SU of maize RuBisCO/sequence of 22 amino acids of the mature part of the SSU of maize RuBisCO/AroA gene fusion:
Similarly, a 228-by EcoRI-HindII fragment ofthe cDNA ofthe SSU ofthe maize RuBisCO gene is ligated with the Klenow polymerise-treated NcoI end of the AroA gene from pRPA-BL 104 and recut with EcoRI. A translational fusion is obtained between the transit peptide of the SSU of maize RuBisCO, the 22 amino acids of the mature part of the SSU of maize RuBisCO and the bacterial EPSPS gene.
Transit peptide of the SSU of sunflower RuBisCO:
The fragment is derived from the cDNA isolated by Waksman and Freyssinet (1987, Nucleic Acids Research 1 S(3), 1328). An SphI site was created at the cleavage site of the transit peptide according to the method of Zoller and Smith (1984, DNA 3(6), 479-488). The transit peptide ofthe S SU of sunflower RuBisCO thus obtained is a 171-by EcoRI-SphI fragment.
Transit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids of the mature part of the SSU of maize RuBisCO/AroA gene fusion:
The construct containing the transit peptide of the SSU of maize RuBisCO/sequence of 22 amino acids of the SSU of maize RuBisCO of the mature part of the maize gene fusion was cut with 171-by EcoRI-SphI corresponding to the transit peptide of the SSU of sunflower RuBisCO. A resulting construct exhibits a ICd:md substitution of the EcoRI-SphI fragments and is a translational fusion "transit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids of the mature part of the SSU of maize RuBisCO/AroA gene.
The EcoRI-SalI fragment was ligated with the SalI-SstI fragment containing the 3' nos sequence and the right end of the T-DNA. The resulting EcoRI-Sstl fragment, comprising "transit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids of the mature part of the SSU of maize RuBisCO/AroA gene/3' nos/T-DNA right end", is substituted for the EcoRI-SstI
fragment containing the right end of the T-DNA of the plasmid 150 A alpha 2 containing the double CaMV promoter. The transcriptional fusion "double CalVlVltransit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids of the mature part of the SSU of maize RuBisCO/AroA gene/3' nos" in the vector A alpha 2 was called pRPA-BL 294. "ransit Deyide of the ~~j,j of sctnflower R_l3BisC0/seat~~nce of 22 amino acids of the SSU of maize, ~t131sC0/transit~tide of the SSt.j o~ ta'ze F3uBisCO/AroA gene" ~sio~:
The above construct is cut with NcoI-HindIII, releasing the AroA
gene. Next it is ligated with a 1.5 kbp NcoI-HindIII fragment containing the "transit peptide of the SSU of maize RuBisCO/AroA gene" fusion. A resulting construct exhibits a substitution of the NcoI-HindIII fragments and is a translational fusion "transit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids of the SSU of the RuBisCO of the mature part of the maize gene/transit peptide of the SSU
of maize RuBisCO/AroA gene".
The EcoRI-SaII fragment was ligated with the SaII-SstI fragment containing the 3' nos sequence and the right end of the T-DNA. The resulting EcoRI
SstI fragment comprising "transit peptide of the SSU of sunflower ' 9 RuBisCO/sequence of 22 amino acids of the S SU of the RuBisCO of the mature part ofthe maize gene/ transit peptide of the SSU of maize RuBisCO/AroA gene/3' nos/T-DNA right end" is substituted for the EcoRI-SstI fragment containing the right end ofthe T-DNA
ofthe plasmid 150 A alpha 2 containing the double CaMV promoter. The transcriptional fusion "double CaMV/transit peptide of the SSU of sunflower RuBisCO/sequence of 22 amino acids ofthe SSU
of the RuBisCO of the mature part of the maize gene/transit peptide of the SSU
of maize RuBisCO/AroA gene/3' nos" in the vector 150 A alpha 2 was called pRPA-BL 410.
EXAMPLE 2: RESISTANCE OF THE TRANSFORMED PLANTS
1. Transformation:
The vector is introduced into the nononcogenic agrobacterium strain EHA 101 (Hood et al., 1987, J. Bacteriol, 168(3), 1291-1301) carrying the cosmid pTVK
291 (Komari et al., 1986, Journal of Bacteriology, 166(1), 88-94). The transformation method is based on the procedure ofHorsch et al. (1985, Science 227(4691), 1229-1231).
2. Regeneration:
The regeneration ofthe tobacco PBD6 (source SEITAFrance) using foliar explants is carried out on a Murashige and Skoog (MS) basic medium containing 30 g/1 of sucrose and 200 g/ml of kanamycin. The foliar explants are removed from greenhouse- or in vitro-grown plants and transformed according to the foliar disc method (Horsch et al., Science 1985, Vol. 227, p.
1229-1231) in three successive stages: the first comprises the induction of shoots on an MS
medium supplemented with 30 g/1 of sucrose containing 0.05 mg/1 of naphthylacetic acid (ANA) and 2 mg/1 of benzylaminopurine (BAP), for 15 days. The shoots formed during this stage are then developed by culturing on an MS medium supplemented with 30 g/1 of sucrose, but not containing hormone, for 10 days. The developed shoots are then removed and Icd:md they are cultured on an MS planting medium containing half the content of salts, vitamins and sugars and not nontaining hormone. After about 15 days, the deeply-rooted shoots are placed in soi;:.
3. Measurement of the glyphosate tolerance:
5 a) In vitro: the tolerance is measured by weighing the mass of calluses extrapolated to 100 foliar discs of 0.5 cm in diameter, after 30 days of growth on an MS medium supplemented with 30 g/1 of sucrose, 0.05 mg/1 of naphthaleneacetic acid and 2 mg/1 of H;AP containing 35 ppm of glyphosate and 200 micrograms/ml of kanamycin. Under these conditions, it is observed that for the tobacco plants L 0 modified by the chimeric gene of pRPA BL 410 according to the invention, the mass of calluses is 34 g whereas for the plants modified by the chimeric gene without a second transit peptide, the mass: is only 12 g.
b) In vivo: 30 plants derived from the regeneration of the tobaccos transformed using pF;PA-BL 2!~4 and pF;PA-BL 410 respectively are transferred to a T.S greenhouse and treat~:.d at the 5-leaf stage by spraying with an aqueous suspension at a dose corresponding to 0.6 kg/ha of glyphosate (Rounds up). After 21 days, a phenotypic examination is carried out of the plants relative to untransformed control plants. Under these conditions, it is observed that the plants transformed using pRPA-BL 410 possess a negligible phytotoxicity whereas the control plants are completely c:0 destroyed; moreover, the plants transformed using a chimeric gene, which differs from the preceding one by the absence of a second transit peptide, possess a phytotoxicity of not Ifas than 3C'% destruction.
These results clearly show the improvement brought by the use of a chimeric gene according to the invention for the same gene encoding the 25 glyphosate tolerance.
*Trade-mark The transfoan~ed plants according to the invention may be used as parents for producing lines and hybrids having an increased tolerance to glyphosate.
~,m_ In a 3: Spring colaas, Westar cultivar, resistant to glyphosate, were obtained using the method of BOULTER et al., 1990 (Plant Science, 70: 91-99), with pRPA-BL x.10. These plants were resistant to a greenhouse treatment with glyphosate at 400 g a.s/ha, a treatment which destroys nontransgenic plants.

Claims (18)

1. A DNA sequence encoding a plastid-localised transit peptide characterized in that it comprises, in the direction of transcription:
a first DNA sequence which encodes a plastid-localized transit peptide wherein said first sequence is derived from the small subunit of a ribulose 1,5 diphosphate carboxylase oxygenase (RuBisCO) gene of a plant, a second DNA sequence which is derived from a gene wherein said second DNA sequence encodes a part of the N-terminal of a mature small subunit of the RuBisCO of a plant, and a third DNA sequence encoding a plastid-localized transit peptide wherein said third sequence is derived from the small subunit of a RuBisCO gene of a plant.
2. A DNA sequence according to claim 1 characterized in that the first DNA
sequence is derived from the same plant as the third DNA sequence.
3. A DNA sequence according to claim 1 characterized in that the first DNA
sequence and the third DNA sequence are derived from different plants.
4. A DNA sequence according to claim 1 or 2 characterized in that the first DNA
sequence, the third DNA sequence or both are derived from the same plant as the second DNA sequence.
5. A DNA sequence according to claim 1, 2, or 4 characterized in that the first DNA sequence, the third DNA sequence or both are derived from the small subunit of the gene for maize RuBisCO.
6. A DNA sequence according to claim 1, 2, or 4 characterized in that the first DNA sequence, the third DNA sequence or both are derived from the small subunit of the gene for sunflower RuBisCO.
7. A DNA sequence according to claim 1, 2, 3, 4, 5, or 6 characterized in that the second DNA sequence is derived from the small subunit of the gene for maize RuBisCO.
8. A DNA sequence according to claim 1, 2, 3, 4, 5, or 6 characterized in that the second DNA sequence is derived from the small subunit of the gene for sunflower RuBisCO.
9. A DNA sequence according to claim 8 characterized in that the part of the mature N-terminal comprises a peptide sequence of from 10 to 40 amino acids.
10. A DNA sequence according to claim 8 characterized in that the part of the mature N-terminal comprises a peptide sequence of from 18 to 33 amino acids.
11. A DNA sequence encoding a plastid-localized transit peptide characterized in that it comprises, in the direction of transcription, a DNA sequence which encodes for:
a plastid-localized transit peptide from the small subunit of sunflower RuBisCO, a sequence of 22 amino acids from the N-terminal of a mature small subunit of maize RuBisCO, and a plastid-localized transit peptide from the small subunit of maize RuBisCO.
12. A chimeric gene for conferring on plants an increased tolerance to a glyphosate herbicide comprising, in the direction of transcription, a DNA
sequence which comprises a promoter region, a DNA sequence which encodes a plastid-localized transit peptide, a coding sequence for a polypeptide which confers glyphosate tolerance and a 3' untranslated polyadenylation signal region characterized in that the DNA
sequence which encodes the plastid-localized transit peptide comprises a DNA
sequence according to claim 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or 11; and wherein the coding sequence for the peptide which confers glyphosate tolerance is a 5-(enolpyruvyl)shikimate-3-phosphate synthase gene.
13. A chimeric gene according to claim 12 characterized in that the coding sequence for glyphosate tolerance is of bacterial origin.
14. A chimeric gene according to claim 12 characterized in that the coding sequence for glyphosate tolerance is of plant origin.
15. A vector for transforming plants characterized in that it comprises a chimeric gene according to claim 12, 13, or 14.
16. Agrobacterium sp. characterized in that it contains a vector according to claim 15.
17. A transformed plant cell characterized in that it contains a chimeric gene according to claim 12, 13, or 14.
18. A process for constructing a chimeric gene according to claim 12, 13, or characterized in that the DNA sequences which encode the two plastid-localized transit peptides, the DNA sequence which encodes a part of a plastid-localized plant peptide, the DNA sequence which encodes a peptide which confers glyphosate tolerance and the untranslated polyadenylation signal region are isolated and then assembled in the direction of transcription of the DNA sequence which encodes the entire peptide which confers glyphosate tolerance.
CA002061636A 1991-03-05 1992-02-21 Chimeric gene for the transformation of plants Expired - Lifetime CA2061636C (en)

Applications Claiming Priority (2)

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FR9102872 1991-03-05
FR9102872A FR2673643B1 (en) 1991-03-05 1991-03-05 TRANSIT PEPTIDE FOR THE INSERTION OF A FOREIGN GENE INTO A PLANT GENE AND PLANTS TRANSFORMED USING THIS PEPTIDE.

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CA2061636A1 CA2061636A1 (en) 1992-09-06
CA2061636C true CA2061636C (en) 2002-12-24

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362396B1 (en) * 1992-03-05 2002-03-26 Aventis Crop Science Chimeric gene for the transformation of plants
FR2734842B1 (en) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie DNA SEQUENCE OF A HYDROXY-PHENYL PYRUVATE DIOXYGENASE GENE AND OBTAINING PLANTS CONTAINING A HYDROXY-PHENYL PYRUVATE DIOXYGENASE GENE, TOLERANT TO CERTAIN HERBICIDES
FR2734840B1 (en) * 1995-06-02 1997-08-01 Rhone Poulenc Agrochimie GENE OF HYDROXY-PHENYL PYRUVATE DIOXYGENASE AND PRODUCTION OF PLANTS CONTAINING THIS GENE RESISTANT TO HERBICIDES
FR2734841B1 (en) * 1995-06-02 1998-03-13 Rhone Poulenc Agrochimie GENE OF HYDROXY-PHENYL PYRUVATE DIOXYGENASE AND PRODUCTION OF PLANTS CONTAINING THIS GENE RESISTANT TO HERBICIDES
FR2736926B1 (en) * 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie 5-ENOL PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE MUTEE, CODING GENE FOR THIS PROTEIN AND PROCESSED PLANTS CONTAINING THIS GENE
FR2736929B1 (en) * 1995-07-19 1997-08-22 Rhone Poulenc Agrochimie ISOLATED DNA SEQUENCE THAT MAY SERVE AS A REGULATION ZONE IN A CHIMERIC GENE FOR USE IN PLANT TRANSFORMATION
DE19531783C2 (en) * 1995-08-30 1999-12-02 Ahmed Sheriff Process to increase plant seed production and to accelerate plant growth through additional plastid pyruvate, phosphate dikinase
EP0801134A1 (en) 1996-04-11 1997-10-15 Hoechst Schering AgrEvo GmbH Process for the production of plants with enhanced growth characteristics
FR2751347B1 (en) 1996-07-16 2001-12-07 Rhone Poulenc Agrochimie CHIMERIC GENE WITH MULTIPLE HERBICIDE TOLERANCE GENES, PLANT CELL AND PLANT TOLERANT WITH MULTIPLE HERBICIDES
US5912332A (en) * 1996-07-26 1999-06-15 Hybridon, Inc. Affinity-based purification of oligonucleotides using soluble multimeric oligonucleotides
US6239332B1 (en) 1996-12-05 2001-05-29 Queen's University At Kingston Constructs and methods for enhancing protein levels in photosynthetic organisms
FR2766207B1 (en) * 1997-07-11 2000-12-08 Rhone Poulenc Agrochimie CHIMERIC GENE ENCODING DROSOMYCIN, VECTOR CONTAINING THE SAME FOR THE TRANSFORMATION OF VEGETABLE CELLS AND OBTAINED TRANSFORMED PLANTS WITH DISEASE RESISTANCE
FR2766206A1 (en) * 1997-07-11 1999-01-22 Rhone Poulenc Agrochimie Chimeric gene containing DNA for drosomicin
US7161064B2 (en) * 1997-08-12 2007-01-09 North Carolina State University Method for producing stably transformed duckweed using microprojectile bombardment
FR2767537B1 (en) * 1997-08-20 2001-07-13 Rhone Poulenc Agrochimie GENE ENCODING ANDROCTONIN, VECTOR CONTAINING SAME AND OBTAINED TRANSFORMED PLANTS RESISTANT TO DISEASE
US6204436B1 (en) 1997-10-31 2001-03-20 Novartis Ag Transgenic plants
FR2772787B1 (en) * 1997-12-24 2001-12-07 Rhone Poulenc Agrochimie H3C4 PROMOTER OF BUT ASSOCIATED WITH THE FIRST INTRON OF RICE ACTINE, CHIMERIC GENE INCLUDING IT AND TRANSFORMED PLANT
US6153811A (en) 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6066786A (en) 1998-06-17 2000-05-23 Pure Seed Testing, Inc. Glyphosate tolerant fescue grasses
WO2000012732A2 (en) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Organelle targeting sequences
US20050086718A1 (en) 1999-03-23 2005-04-21 Mendel Biotechnology, Inc. Plant transcriptional regulators of abiotic stress
US7196245B2 (en) 2002-09-18 2007-03-27 Mendel Biotechnology, Inc. Polynucleotides and polypeptides that confer increased biomass and tolerance to cold, water deprivation and low nitrogen to plants
US7897843B2 (en) 1999-03-23 2011-03-01 Mendel Biotechnology, Inc. Transcriptional regulation of plant biomass and abiotic stress tolerance
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6384304B1 (en) * 1999-10-15 2002-05-07 Plant Genetic Systems N.V. Conditional sterility in wheat
EP1230344B1 (en) 1999-11-17 2011-11-02 Mendel Biotechnology, Inc. Plant biochemistry-related genes
EP1950306A1 (en) 1999-11-17 2008-07-30 Mendel Biotechnology, Inc. Environmental stress tolerance genes
FR2803592A1 (en) * 2000-01-06 2001-07-13 Aventis Cropscience Sa NOVEL DERIVATIVES OF 3-HYDROXYPICOLINIC ACID, PROCESS FOR THEIR PREPARATION AND FUNGICIDAL COMPOSITIONS CONTAINING SAME
EP2410060A1 (en) 2000-08-22 2012-01-25 Mendel Biotechnology, Inc. Genes for modifying plant traits IV
CA2422649A1 (en) * 2000-09-08 2002-03-14 Bayer Cropscience Sa Hydroxy-phenyl pyruvate dioxygenase fused with a signal peptide, dna sequence and use for obtaining plants containing herbicide-tolerant plants
PL366144A1 (en) * 2000-10-30 2005-01-24 Maxygen, Inc. Novel glyphosate n-acetyltransferase (gat) genes
FR2815969B1 (en) 2000-10-30 2004-12-10 Aventis Cropscience Sa TOLERANT PLANTS WITH HERBICIDES BY METABOLIC BYPASS
US7462481B2 (en) * 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
US20090011938A1 (en) * 2000-10-30 2009-01-08 Pioneer Hi--Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
PT1988099E (en) 2001-01-09 2013-01-31 Bayer Cropscience Nv Bacillus thuringiensis insecticidal proteins
DE60238904D1 (en) * 2001-02-22 2011-02-24 Biogemma Fr CONSTITUTIVE PROMOTER FROM ARABIDOPSIS
EP1465988A4 (en) 2001-03-19 2005-08-03 Cargill Inc Myo-inositol oxygenases
ATE530654T1 (en) 2001-07-06 2011-11-15 Monsanto Technology Llc METHOD FOR PROMOTING THE SEPARATION OF TRANSGENES IN PLANTS AND COMPOSITIONS THEREFOR
US8022272B2 (en) * 2001-07-13 2011-09-20 Sungene Gmbh & Co. Kgaa Expression cassettes for transgenic expression of nucleic acids
AU2002334950A1 (en) * 2001-10-26 2003-05-12 Genencor International, Inc. T. reesei phytase enzymes, polynucleides encoding the enzymes, vectors and host cells thereof, and methods of using
EP1438417B1 (en) * 2001-10-26 2014-05-14 Danisco US Inc. Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same
EP1481068B1 (en) 2002-02-26 2011-02-02 Syngenta Limited A method of selectively producing male or female sterile plants
CA2911801A1 (en) 2002-03-22 2003-10-02 Greta Arnaut Novel bacillus thuringiensis insecticidal proteins
CA2851035C (en) 2002-07-18 2018-05-29 Stanislaw Flasinski Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
ATE546539T1 (en) 2002-07-26 2012-03-15 Basf Plant Science Gmbh REVERSING THE NEGATIVE-SELECTIVE EFFECT OF NEGATIVE MARKER PROTEINS AS A SELECTION METHOD
AU2003258157A1 (en) * 2002-08-12 2004-02-25 Genencor International, Inc. Mutant e. coli appa phytase enzymes
US7045684B1 (en) * 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
FR2848570B1 (en) 2002-12-12 2005-04-01 Bayer Cropscience Sa EXPRESSION CASSETTE ENCODING A 5-ENOL PYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE (EPSPS) AND HERBICIDE TOLERANT PLANTS CONTAINING THE SAME
EP1620557A2 (en) 2003-04-29 2006-02-01 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
WO2005001101A1 (en) 2003-06-03 2005-01-06 University Of Georgia Research Foundation, Inc. Conditional sterility in plants
WO2005070088A2 (en) * 2004-01-15 2005-08-04 University Of Georgia Research Foundation, Inc. Chimeric sequences for tissue-specific gene expression in plants
US7405074B2 (en) * 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
WO2006023869A2 (en) 2004-08-24 2006-03-02 Monsanto Technology Llc Adenylate translocator protein gene non-coding regulatory elements for use in plants
BRPI0518249A2 (en) * 2004-10-29 2008-11-11 Bayer Bioscience Nv strain tolerant cotton plants
EP1679374A1 (en) 2005-01-10 2006-07-12 Bayer CropScience GmbH Transformed plant expressing a mutansucrase and synthesizing a modified starch
ES2435079T3 (en) 2005-07-08 2013-12-18 Universidad Nacional Autonoma De Mexico Instituto New bacterial proteins with pesticide activity
AU2006283504B2 (en) * 2005-08-24 2011-08-25 E. I. Du Pont De Nemours And Company Compositions providing tolerance to multiple herbicides and methods of use thereof
CN101437843B (en) 2006-01-23 2013-08-07 密歇根州立大学评议会 Methods for breeding glyphosate resistant plants and compositions thereof
EP1996009A4 (en) * 2006-03-02 2009-09-30 Athenix Corp Methods and compositions for improved enzyme activity in transgenic plant
CA2646471C (en) 2006-03-21 2016-05-31 Bayer Bioscience N.V. Novel genes encoding insecticidal proteins
CA2651428A1 (en) * 2006-05-12 2007-11-22 Commonwealth Scientific And Industrial Research Organisation Enzymes for degrading herbicides
US9045765B2 (en) 2006-06-09 2015-06-02 Athenix Corporation EPSP synthase genes conferring herbicide resistance
MX2008015557A (en) * 2006-06-13 2009-01-13 Athenix Corp Improved epsp synthases: compositions and methods of use.
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
EP2147100B1 (en) 2007-04-27 2017-06-07 The Regents of The University of California Plant co2 sensors, nucleic acids encoding them, and methods for making and using them
CN101688216B (en) * 2007-06-01 2014-03-26 拜尔作物科学公司 Novel genes encoding insecticidal proteins
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
BRPI0907000A2 (en) 2008-02-01 2015-07-07 Athenix Corp Directed evolution of grg31 and grg36 epsp synthase enzymes
EP2607488B1 (en) 2008-04-07 2016-11-02 Monsanto Technology LLC Plant regulatory elements and uses thereof
US7935870B2 (en) * 2008-05-14 2011-05-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV354718
US8829282B2 (en) * 2008-05-14 2014-09-09 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV425044
US7947877B2 (en) * 2008-05-14 2011-05-24 Monosanto Technology LLC Plants and seeds of spring canola variety SCV328921
US7964774B2 (en) * 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
EP3441470A1 (en) 2008-09-26 2019-02-13 BASF Agrochemical Products, B.V. Herbicide-resistant ahas-mutants and methods of use
US9074005B2 (en) * 2009-01-02 2015-07-07 Washington State University Compositions and methods for modulating plant disease resistance and immunity
US8471100B2 (en) * 2009-05-15 2013-06-25 University Of Tennessee Research Foundation Environmental stress-inducible promoter and its application in crops
US8466342B2 (en) 2009-06-09 2013-06-18 Pioneer Hi Bred International Inc Early endosperm promoter and methods of use
US8071848B2 (en) * 2009-06-17 2011-12-06 Monsanto Technology Llc Plants and seeds of spring canola variety SCV218328
EA201270107A1 (en) 2009-07-01 2012-07-30 Байер Байосайенс Н.В. METHODS AND MEANS FOR OBTAINING PLANTS WITH IMPROVED RESISTANCE TO GLYPHOSATE
EP2275564A1 (en) 2009-07-17 2011-01-19 Freie Universität Berlin Means and method for the production of transgenic plants that are resistant to clubroot
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
IN2012DN03073A (en) 2009-10-26 2015-07-31 Pioneer Hi Bred Int
WO2011063411A1 (en) 2009-11-23 2011-05-26 Bayer Bioscience N.V. Elite event ee-gm3 and methods and kits for identifying such event in biological samples
US9683242B2 (en) * 2009-11-23 2017-06-20 M.S. Technologies, Llc Herbicide tolerant soybean plants and methods for identifying same
EP2516631B1 (en) 2009-12-23 2018-02-14 Bayer Intellectual Property GmbH Plants tolerant to hppd inhibitor herbicides
AU2010334815B2 (en) 2009-12-23 2015-07-09 Bayer Intellectual Property Gmbh Plants tolerant to HPPD inhibitor herbicides
AR079882A1 (en) 2009-12-23 2012-02-29 Bayer Cropscience Ag TOLERANT PLANTS TO INHIBITING HERBICIDES OF HPPD
WO2011076892A1 (en) 2009-12-23 2011-06-30 Bayer Cropscience Ag Plants tolerant to hppd inhibitor herbicides
AR079972A1 (en) 2009-12-23 2012-03-07 Bayer Cropscience Ag TOLERANT PLANTS TO INHIBITING HERBICIDES OF HPPD
AR079909A1 (en) 2010-01-14 2012-02-29 Monsanto Technology Llc ELEMENTS OF VEGETABLE REGULATION AND ITS USES
AR080021A1 (en) 2010-01-26 2012-03-07 Pioneer Hi Bred Int TOLERANCE TO HPPD INHIBITING HERBICIDES (HYDROPHENYL PIRUVATO DIOXYGENASE)
WO2011095460A1 (en) 2010-02-02 2011-08-11 Bayer Cropscience Ag Soybean transformation using hppd inhibitors as selection agents
MY169476A (en) 2010-02-04 2019-04-12 Bayer Cropscience Ag A method for increasing photosynthetic carbon fixation using glycolate dehydrogenase multi-subunit fusion protein
US8148611B2 (en) * 2010-02-26 2012-04-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV453784
US8138394B2 (en) * 2010-02-26 2012-03-20 Monsanto Technology Llc Plants and seeds of spring canola variety SCV431158
US8143488B2 (en) * 2010-02-26 2012-03-27 Monsanto Technoloy LLC Plants and seeds of spring canola variety SCV470336
EA201792402A3 (en) 2010-03-08 2018-09-28 Монсанто Текнолоджи Ллс POLYNUCLEOTIDE MOLECULES FOR REGULATION OF PLANT GENES
US8581048B2 (en) * 2010-03-09 2013-11-12 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) * 2010-03-11 2012-04-10 Monsanto Technology Llc Plants and seeds of spring canola variety SCV152154
EP2603591A1 (en) 2010-08-13 2013-06-19 Pioneer Hi-Bred International Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity
EP2669372B1 (en) 2010-11-10 2016-07-06 Bayer CropScience AG Hppd variants and methods of use
CA2818918A1 (en) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof
UA122558C2 (en) 2010-11-24 2020-12-10 Піонер Хай-Бред Інтернешнл, Інк. Brassica gat event dp-073496-4 and compositions and methods for the identification and/or detection thereof
BR112013013377A2 (en) 2010-12-03 2016-11-22 Ms Technologies Llc nucleic acid molecule encoding a 5-enolpyruvyl-3-phospho-shikimic acid synthase (epsps), construct, vector, plant cell, plant, method for producing a plant having
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
TWI667347B (en) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 Soybean event syht0h2 and compositions and methods for detection thereof
BR112013014698A2 (en) 2010-12-22 2017-03-07 E I Du Point De Nemours & Company dna construct, vector, plant cell, plant, transgenic plant-derived seed, method for expression of a nucleotide sequence in a plant, method for expression of a nucleotide sequence in a plant cell, method for selectively expressing a nucleotide sequence in corn root, stem, grain and tassel tissues
BR112013014988A2 (en) 2010-12-22 2017-06-27 Du Pont isolated nucleic acid molecule, dna construct, vector, plant cell, plant, transgenic seed from plant, method for expression of a nucleotide sequence in a plant, method for expression of a nucleotide sequence in a plant cell, method for selectively express a nucleotide sequence in green corn plant tissues
EP2675900B1 (en) 2011-02-15 2017-09-06 Pioneer Hi-Bred International, Inc. Root-preferred promoter and methods of use
UA111193C2 (en) 2011-03-25 2016-04-11 Баєр Інтеллекчуел Проперті Гмбх Use of n-(tetrazol-4-yl)- or n-(triazol-3-yl)arylcarboxamides or salts thereof for controlling unwanted plants in areas of transgenic crop plants being tolerant to hppd inhibitor herbicides
BR112013024659A2 (en) 2011-03-25 2018-08-07 Bayer Ip Gmbh use of n- (1,2,5-oxadiazol-3-yl) benzamides to control unwanted plants in transgenic crop areas that are tolerant to hppd inhibitor herbicides
ES2666149T3 (en) 2011-03-25 2018-05-03 Monsanto Technology Llc Regulatory elements of plants and their uses
US8513487B2 (en) 2011-04-07 2013-08-20 Zenon LISIECZKO Plants and seeds of spring canola variety ND-662c
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
US8507761B2 (en) 2011-05-05 2013-08-13 Teresa Huskowska Plants and seeds of spring canola variety SCV372145
US8513495B2 (en) 2011-05-10 2013-08-20 Dale Burns Plants and seeds of spring canola variety SCV291489
EA037364B1 (en) 2011-05-13 2021-03-18 Монсанто Текнолоджи Ллс Plant regulatory elements and uses thereof
EP2714901A1 (en) 2011-05-31 2014-04-09 Keygene N.V. Pest resistant plants
WO2012170304A2 (en) 2011-06-02 2012-12-13 The Regents Of The University Of California Plants with elevated levels of glucan
EP2737067A1 (en) 2011-07-28 2014-06-04 Genective Glyphosate tolerant corn event vco-ø1981-5 and kit and method for detecting the same
MX348003B (en) 2011-08-22 2017-03-08 Bayer Cropscience Nv Methods and means to modify a plant genome.
MX350774B (en) 2011-09-13 2017-09-15 Monsanto Technology Llc Methods and compositions for weed control.
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
AU2012308765B2 (en) 2011-09-13 2018-06-21 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
EP2756085B1 (en) 2011-09-13 2019-03-20 Monsanto Technology LLC Methods and compositions for weed control
UY34333A (en) 2011-09-13 2013-04-30 Monsanto Technology Llc ? METHODS AND COMPOSITIONS FOR WEED CONTROL, AND METHODS TO REDUCE THE EXPRESSION OF ENZYME DHPS?
WO2013040057A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
EP2755467B1 (en) 2011-09-13 2017-07-19 Monsanto Technology LLC Methods and compositions for weed control
AR088155A1 (en) 2011-09-13 2014-05-14 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR WEED CONTROL
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CN104039957A (en) 2011-10-19 2014-09-10 凯金公司 Methods and compositions for producing drimenol
US9204603B2 (en) 2011-12-21 2015-12-08 The Curators Of The University Of Missouri Soybean variety S05-11482
US20130167262A1 (en) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Soybean variety s05-11268
US20130180008A1 (en) 2012-01-06 2013-07-11 Pioneer Hi Bred International Inc Ovule Specific Promoter and Methods of Use
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
UY34607A (en) * 2012-02-01 2013-09-02 Dow Agrosciences Llc GLIFOSATO RESISTANT PLANTS AND ASSOCIATED METHODS.
WO2013127766A1 (en) 2012-02-29 2013-09-06 Bayer Cropscience Nv Als inhibitor herbicide tolerant b. napus mutants
AU2013205557B2 (en) * 2012-04-17 2016-04-21 Corteva Agriscience Llc Synthetic brassica-derived chloroplast transit peptides
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8802935B2 (en) 2012-04-26 2014-08-12 Monsanto Technology Llc Plants and seeds of spring canola variety SCV942568
AR091143A1 (en) 2012-05-24 2015-01-14 Seeds Ltd Ab COMPOSITIONS AND METHODS TO SILENCE GENETIC EXPRESSION
RU2619178C2 (en) 2012-06-07 2017-05-12 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Construct and method of transgene expression using bidirectional constitutive brassica promoter
WO2013188291A2 (en) 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
US10689660B2 (en) 2012-06-22 2020-06-23 The Regents Of The University Of California Compositions and methods for mediating plant stomatal development in response to carbon dioxide and applications for engineering drought tolerance in plants
CA2878289A1 (en) 2012-07-06 2014-01-09 Bayer Cropscience Nv Brassica plants with modified seed oil composition
AU2012208997B1 (en) 2012-07-30 2013-09-19 Dlf Usa Inc. An alfalfa variety named magnum salt
BR112015005674B1 (en) 2012-09-14 2022-09-06 BASF Agricultural Solutions Seed US LLC RECOMBINANT POLYPEPTIDES TO PROVIDE HERBICIDES TOLERANCE
CA2887571A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
EP2908620A4 (en) 2012-10-18 2016-07-27 Monsanto Technology Llc Methods and compositions for plant pest control
UA117816C2 (en) 2012-11-06 2018-10-10 Байєр Кропсайєнс Акцієнгезелльшафт Herbicidal combinations for tolerant soybean cultures
CA2894213A1 (en) 2012-12-21 2014-06-26 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
EP2941488B1 (en) 2013-01-01 2023-03-22 Monsanto Technology LLC Methods of introducing dsrna to plant seeds for modulating gene expression
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
AU2014211570A1 (en) 2013-01-29 2015-07-23 The University Court Of The University Of Glasgow Methods and means for increasing stress tolerance and biomass in plants
RU2723717C2 (en) 2013-03-07 2020-06-17 Атеникс Корп. Toxins genes and methods of using them
US9243258B2 (en) 2013-03-12 2016-01-26 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US9273322B2 (en) 2013-03-12 2016-03-01 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
MX364458B (en) 2013-03-13 2019-04-26 Monsanto Technology Llc Methods and compositions for weed control.
AU2014241045B2 (en) 2013-03-13 2017-08-31 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
EP2967082A4 (en) 2013-03-13 2016-11-02 Monsanto Technology Llc Methods and compositions for weed control
EP2970363B1 (en) 2013-03-14 2020-07-08 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20160053277A1 (en) 2013-03-14 2016-02-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
US20160040149A1 (en) 2013-03-14 2016-02-11 Pioneer Hi-Bred International Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
CN105473605A (en) 2013-03-15 2016-04-06 先锋国际良种公司 Phi-4 polypeptides and methods for their use
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
EA201591922A1 (en) 2013-04-05 2016-04-29 Байер Кропсайенс Н.В. BRASSICA PLANTS INCLUDING DA1 MUTANT ALLIES
EP3017049B1 (en) 2013-07-01 2018-08-22 Bayer CropScience NV Methods and means for modulating flowering time in monocot plants
EP3019617A1 (en) 2013-07-12 2016-05-18 Bayer CropScience NV Als inhibitor herbicide tolerant mutant plants
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
MX359191B (en) 2013-07-19 2018-09-18 Monsanto Technology Llc Compositions and methods for controlling leptinotarsa.
CA2918909A1 (en) 2013-07-25 2015-01-29 Pioneer Hi-Bred International, Inc. Method for producing hybrid brassica seed
EP3032942B1 (en) 2013-08-16 2020-03-11 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20160201073A1 (en) 2013-09-11 2016-07-14 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN105874062B (en) 2013-10-18 2021-07-20 先锋国际良种公司 Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use
CA2923296A1 (en) 2013-10-25 2015-04-30 Pioneer Hi-Bred International, Inc. Stem canker tolerant soybeans and methods of use
AR098295A1 (en) 2013-11-04 2016-05-26 Monsanto Technology Llc COMPOSITIONS AND METHODS TO CONTROL INFESTATIONS OF PESTS AND PARASITES OF THE ARTHROPODS
UA119253C2 (en) 2013-12-10 2019-05-27 Біолоджикс, Інк. Compositions and methods for virus control in varroa mite and bees
AR099092A1 (en) 2014-01-15 2016-06-29 Monsanto Technology Llc METHODS AND COMPOSITIONS FOR WEED CONTROL USING EPSPS POLYUCLEOTIDES
CN114763376A (en) 2014-02-07 2022-07-19 先锋国际良种公司 Insecticidal proteins and methods of use thereof
BR112016018287A2 (en) 2014-02-07 2017-10-10 Du Pont insecticide proteins and methods for their use
BR112016020889B1 (en) 2014-03-11 2022-10-04 BASF Agricultural Solutions Seed US LLC RECOMBINANT NUCLEIC ACID MOLECULE, BACTERIAL HOST CELL, RECOMBINANT HPPD PROTEIN, RECOMBINANT NUCLEIC ACID USE AND BASE PRODUCT
US11091770B2 (en) 2014-04-01 2021-08-17 Monsanto Technology Llc Compositions and methods for controlling insect pests
US10053702B2 (en) 2014-04-22 2018-08-21 E I Du Pont De Nemours And Company Plastidic carbonic anhydrase genes for oil augmentation in seeds with increased DGAT expression
AR100874A1 (en) 2014-06-16 2016-11-09 Consejo Nac De Investig Científicas Y Técnicas (Conicet) CHEMICAL GENES AND PROTEINS OF OXIDATIVE RESISTANCE, AND TRANSGENIC PLANTS THAT INCLUDE THE SAME
US10988764B2 (en) 2014-06-23 2021-04-27 Monsanto Technology Llc Compositions and methods for regulating gene expression via RNA interference
WO2015200539A1 (en) 2014-06-25 2015-12-30 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
US9686931B2 (en) 2014-07-07 2017-06-27 Alforex Seeds LLC Hybrid alfalfa variety named HybriForce-3400
US10378012B2 (en) 2014-07-29 2019-08-13 Monsanto Technology Llc Compositions and methods for controlling insect pests
CA2955828A1 (en) 2014-08-08 2016-02-11 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
CA2962242A1 (en) 2014-09-29 2016-04-07 Agrigenetics, Inc. Low lignin non-transgenic alfalfa varieties and methods for producing the same
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
US10487123B2 (en) 2014-10-16 2019-11-26 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
CN113372421A (en) 2014-10-16 2021-09-10 先锋国际良种公司 Insecticidal proteins and methods of use thereof
KR102127553B1 (en) 2014-10-16 2020-06-29 몬산토 테크놀로지 엘엘씨 Novel chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
US10316329B2 (en) 2014-10-16 2019-06-11 Monsanto Technology Llc Proteins toxic or inhibitory to lepidopteran insects
MA41180A (en) 2014-12-17 2017-10-24 Bayer Cropscience Nv PLANTS CHARACTERIZED BY IMPROVED PHOTOSYNTHETIC CARBON BINDING CAPACITY
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
CA2974101A1 (en) 2015-01-22 2016-07-28 Monsanto Technology Llc Compositions and methods for controlling leptinotarsa
WO2016128519A1 (en) 2015-02-12 2016-08-18 Bayer Cropscience Nv Shoot apex-preferential promoters and uses thereof
CA2983893A1 (en) 2015-04-28 2016-11-03 Bayer Cropscience Nv Brassica plants with modified seed oil composition
WO2016186986A1 (en) 2015-05-19 2016-11-24 Pioneer Hi Bred International Inc Insecticidal proteins and methods for their use
CN107750125A (en) 2015-06-02 2018-03-02 孟山都技术有限公司 For by the composition and method in delivery of polynucleotides to plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
CA2986265A1 (en) 2015-06-16 2016-12-22 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US11198709B2 (en) 2015-08-06 2021-12-14 E. I. Du Pont De Nemours And Company Plant derived insecticidal proteins and methods for their use
EP3332010A1 (en) 2015-08-07 2018-06-13 Bayer CropScience NV Root-preferential and stress inducible promoter and uses thereof
CN114058631A (en) 2015-08-27 2022-02-18 孟山都技术公司 Novel insect inhibitory proteins
AU2016318051B2 (en) 2015-09-04 2022-11-03 Keygene N.V. Diplospory gene
BR112018004779A8 (en) 2015-09-11 2022-08-09 Bayer Cropscience Lp VARIANTS OF HPPD AND METHODS OF USE
CN108292328B (en) 2015-11-10 2022-04-19 美国陶氏益农公司 Methods and systems for predicting risk of transgene silencing
WO2017102923A1 (en) 2015-12-15 2017-06-22 Bayer Cropscience Nv Brassicaceae plants resistant to plasmodiophora brassicae (clubroot)
EP4257694A3 (en) 2015-12-22 2023-12-06 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
BR112018016057A2 (en) 2016-02-05 2019-01-29 Pioneer Hi Bred Int methods for selecting a soybean plant or soybean germplasm with enhanced resistance to brown stem rot infection and kit
US10975380B2 (en) 2016-04-11 2021-04-13 Basf Agricultural Solutions Seed, Us Llc Seed-specific and endosperm-preferental promoters and uses thereof
US20190127747A1 (en) 2016-04-11 2019-05-02 Bayer Cropscience Nv Seed-specific and endosperm-preferential promoters and uses thereof
AU2017249365B2 (en) 2016-04-13 2023-04-20 BASF Agricultural Solutions Seed US LLC Seed- and funiculus-preferential promoters and uses thereof
WO2017178368A1 (en) 2016-04-13 2017-10-19 Bayer Cropscience Nv Seed-specific and embryo-preferential promoters and uses thereof
AU2017253562B2 (en) 2016-04-20 2023-02-02 BASF Agricultural Solutions Seed US LLC Elite event EE-GH7 and methods and kits for identifying such event in biological samples
EA201892293A1 (en) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION
US20190185867A1 (en) 2016-06-16 2019-06-20 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20190194676A1 (en) 2016-06-24 2019-06-27 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
US20190359992A1 (en) 2016-06-28 2019-11-28 Cellectis Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
EP3478052B1 (en) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN115885615A (en) 2016-08-22 2023-04-04 拜欧卢米克有限公司 Seed treatment system, device and method
US11021716B2 (en) 2016-11-01 2021-06-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2017365169B2 (en) 2016-11-23 2022-07-21 BASF Agricultural Solutions Seed US LLC Axmi669 and Axmi991 toxin genes and methods for their use
AU2017382305A1 (en) 2016-12-22 2019-07-18 BASF Agricultural Solutions Seed US LLC Use of CRY14 for the control of nematode pests
WO2018136611A1 (en) 2017-01-18 2018-07-26 Bayer Cropscience Lp Use of bp005 for the control of plant pathogens
EP3571303A1 (en) 2017-01-18 2019-11-27 Basf Agricultural Solutions Seed Us Llc Bp005 toxin gene and methods for its use
US9961859B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 57111348
US9999189B1 (en) 2017-02-28 2018-06-19 M.S. Technologies, Llc Soybean cultivar 54113122
US10058049B1 (en) 2017-02-28 2018-08-28 M.S. Technologies Llc Soybean cultivar 59104161
US9867357B1 (en) 2017-02-28 2018-01-16 M.S. Technologies, Llc Soybean cultivar 56171900
US9961861B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 54190212
US9999190B1 (en) 2017-02-28 2018-06-19 M.S. Technologies, Llc Soybean cultivar 54172927
US9961860B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 52030201
US9961858B1 (en) 2017-02-28 2018-05-08 M.S. Technologies, Llc Soybean cultivar 54062650
WO2018165091A1 (en) 2017-03-07 2018-09-13 Bayer Cropscience Lp Hppd variants and methods of use
EP3601578A1 (en) 2017-03-23 2020-02-05 Basf Se Anther-specific promoter and uses thereof
AU2018294213A1 (en) 2017-06-26 2019-12-12 Basf Se Regeneration of cereals
WO2019038594A2 (en) 2017-08-21 2019-02-28 Biolumic Limited High growth and high hardiness transgenic plants
KR20200056434A (en) 2017-09-25 2020-05-22 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 Tissue-preferred promoters and methods of use
BR112020008092A2 (en) 2017-10-24 2020-09-15 BASF Agricultural Solutions Seed US LLC method for checking tolerance to a GM herbicide and soy plant
WO2019083808A1 (en) 2017-10-24 2019-05-02 Basf Se Improvement of herbicide tolerance to hppd inhibitors by down-regulation of putative 4-hydroxyphenylpyruvate reductases in soybean
US20200332311A1 (en) 2018-01-12 2020-10-22 The Texas A&M University System Increasing plant bioproduct yield
US10485208B1 (en) 2018-05-21 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 69311428
US10501747B1 (en) 2018-05-21 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 77130123
US10485209B1 (en) 2018-05-21 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 72151329
US10494639B1 (en) 2018-05-21 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 63301112
US10499594B1 (en) 2018-05-21 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 79162140
CA3096516A1 (en) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP3814490A2 (en) 2018-06-27 2021-05-05 Basf Se Thermostable rubisco activase and uses thereof
CA3097915A1 (en) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Methods for selecting transformed plants
US10485211B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 74142136
US10555478B1 (en) 2018-08-01 2020-02-11 M.S. Technologies, L.L.C. Soybean cultivar 75242840
US10492401B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 64002217
US10499582B1 (en) 2018-08-01 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 60312840
US10485210B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 64432136
US10455793B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 69090024
US10492402B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 63452016
US10455794B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 51284052
US10492400B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 76011212
US10492403B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70311819
US10448604B1 (en) 2018-08-01 2019-10-22 M.S. Technologies, L.L.C. Soybean cultivar 60111110
US10455795B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 79150907
US10455796B1 (en) 2018-08-01 2019-10-29 M.S. Technologies, L.L.C. Soybean cultivar 76420724
US10485212B1 (en) 2018-08-01 2019-11-26 M.S. Technologies, L.L.C. Soybean cultivar 65110742
US10492404B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 61332840
US10492399B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 75001212
US10492433B1 (en) 2018-08-01 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 78492244
US10555479B1 (en) 2018-08-01 2020-02-11 M.S. Technologies, L.L.C. Soybean cultivar 71342318
US10499596B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 76034331
US10440925B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 61414428
US10492439B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 78281713
US10492437B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 73081781
US10499597B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 74211709
US10531620B1 (en) 2018-08-02 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar 70140849
US10492436B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70422534
US10542691B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 73040436
US10398121B1 (en) 2018-08-02 2019-09-03 M.S. Technologies, Llc Soybean cultivar 76132184
US10524445B1 (en) 2018-08-02 2020-01-07 M.S. Technologies, L.L.C. Soybean cultivar 75052534
US10537085B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 73330613
US10537084B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 71201428
US10492438B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 72191315
US10499583B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 73390208
US10548271B1 (en) 2018-08-02 2020-02-04 M.S. Technologies, L.L.C. Soybean cultivar 65180532
US10542692B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 70262703
US10440926B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 75251428
US10506783B1 (en) 2018-08-02 2019-12-17 M.S. Technologies, L.L.C. Soybean cultivar 70391206
US10492440B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 76172605
US10499595B1 (en) 2018-08-02 2019-12-10 M.S. Technologies, L.L.C. Soybean cultivar 77242824
US10542690B1 (en) 2018-08-02 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 71052129
US10398120B1 (en) 2018-08-02 2019-09-03 M.S. Technologies, L.L.C. Soybean cultivar 70271905
US10448605B1 (en) 2018-08-02 2019-10-22 M.S. Technologies, L.L.C. Soybean cultivar 63332027
US10440924B1 (en) 2018-08-02 2019-10-15 M.S. Technologies, L.L.C. Soybean cultivar 60431428
US10512229B1 (en) 2018-08-02 2019-12-24 M.S. Technologies, L.L.C. Soybean cultivar 74340613
US10349605B1 (en) 2018-08-02 2019-07-16 M.S. Technologies, Llc Soybean cultivar 78320329
US10537076B1 (en) 2018-08-02 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 70120311
US10349606B1 (en) 2018-08-02 2019-07-16 M.S. Technologies, Llc Soybean cultivar 70404329
US10492441B1 (en) 2018-08-02 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 75162223
US10517246B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 61242247
US10517245B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 67371612
US10542694B1 (en) 2018-08-03 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 77290232
US10631483B2 (en) 2018-08-03 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 63030535
US10660286B2 (en) 2018-08-03 2020-05-26 M.S. Technologies, L.L.C. Soybean cultivar 66472542
US10492442B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 64490328
US10631484B2 (en) 2018-08-03 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 60310209
US10660291B2 (en) 2018-08-03 2020-05-26 M.S. Technologies, L.L.C. Soybean cultivar 76071630
US10542693B1 (en) 2018-08-03 2020-01-28 M.S. Technologies, L.L.C. Soybean cultivar 74312619
US10492443B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 70552824
US10537078B1 (en) 2018-08-03 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 78221232
US10609881B2 (en) 2018-08-03 2020-04-07 M.S. Technologies, L.L.C. Soybean cultivar 74092327
US10517247B1 (en) 2018-08-03 2019-12-31 M.S. Technologies, L.L.C. Soybean cultivar 71270402
US10537077B1 (en) 2018-08-03 2020-01-21 M.S. Technologies, L.L.C. Soybean cultivar 76391606
US10492444B1 (en) 2018-08-03 2019-12-03 M.S. Technologies, L.L.C. Soybean cultivar 73412247
US10531621B1 (en) 2018-08-03 2020-01-14 M.S. Technologies, L.L.C. Soybean cultivar 61364961
US10477819B1 (en) 2018-08-06 2019-11-19 M.S. Technologies, L.L.C. Soybean cultivar 75162339
EP3874050A1 (en) 2018-10-31 2021-09-08 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
WO2020101187A1 (en) * 2018-11-15 2020-05-22 주식회사 바이오앱 Recombinant vector for expressing virus-like particles in plant and method for preparation of vaccine composition containing circovirus-like particles by using same
CN113412333A (en) 2019-03-11 2021-09-17 先锋国际良种公司 Method for clonal plant production
CA3128376A1 (en) 2019-03-27 2020-10-01 Pioneer Hi-Bred International, Inc. Plant explant transformation
CA3127173A1 (en) 2019-03-28 2020-10-01 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
US10631511B1 (en) 2019-04-04 2020-04-28 M.S. Technologies, L.L.C. Soybean cultivar 83190332
US10667483B1 (en) 2019-04-22 2020-06-02 M.S. Technolgies, L.L.C. Soybean cultivar 88092742
WO2020239984A1 (en) 2019-05-29 2020-12-03 Keygene N.V. Gene for parthenogenesis
US10595486B1 (en) 2019-06-13 2020-03-24 M.S. Technologies, L.L.C. Soybean cultivar 80330329
WO2021004838A2 (en) 2019-07-05 2021-01-14 BASF Agricultural Solutions Seed US LLC Rubisco activase with reduced adp inhibition and uses thereof
US10993402B2 (en) 2019-08-19 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 87390112
US10897867B1 (en) 2019-08-19 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 84450325
US10966396B2 (en) 2019-08-19 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 89192414
US10939651B1 (en) 2019-08-19 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 83011212
US10993403B2 (en) 2019-08-19 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 88042312
US10980205B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 81140111
US10980207B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 87242903
US10980204B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 85010111
US10945399B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 89442841
US10918064B1 (en) 2019-08-19 2021-02-16 M.S. Technologies, L.L.C. Soybean cultivar 84322401
US10952394B2 (en) 2019-08-19 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88390016
US10973197B2 (en) 2019-08-19 2021-04-13 M.S. Technologies, L.L.C. Soybean cultivar 87161800
US10897866B1 (en) 2019-08-19 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 81442208
US10932431B1 (en) 2019-08-19 2021-03-02 M.S. Technologies, L.L.C. Soybean cultivar 86072910
US10980206B2 (en) 2019-08-19 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 86052115
US10952395B2 (en) 2019-08-19 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 81371335
US10945401B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 83372609
US10945400B1 (en) 2019-08-19 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 86220335
US11044870B2 (en) 2019-08-19 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 87011338
US10945403B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 85202128
US11337394B2 (en) 2019-08-20 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 86092833
US10952397B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 86160724
US11044871B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 84340383
US11212998B2 (en) 2019-08-20 2022-01-04 M.S. Technologies, L.L.C. Soybean cultivar 88282833
US11044874B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 83292541
US10952399B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 80230701
US11044875B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 81322943
US11044872B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 84344663
US10945402B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 88020223
US10952398B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 84380724
US10932432B1 (en) 2019-08-20 2021-03-02 M.S. Technologies, L.L.C. Soybean cultivar 87222215
US11044873B2 (en) 2019-08-20 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 88482541
US10952396B2 (en) 2019-08-20 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88362310
US10945404B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 83221630
US11172632B2 (en) 2019-08-20 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 85031644
US10945405B1 (en) 2019-08-20 2021-03-16 M.S. Technologies, L.L.C. Soybean cultivar 81090603
US11219179B2 (en) 2019-08-20 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 87272833
US11006604B2 (en) 2019-08-27 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 82431018
US10993405B2 (en) 2019-08-27 2021-05-04 M.S. Technologies, L.L.C. Soybean cultivar 85281832
US11006605B2 (en) 2019-08-27 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 84410120
US11071273B2 (en) 2019-08-27 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 86172030
US10897871B1 (en) 2019-08-27 2021-01-26 M.S. Technologies, L.L.C. Soybean cultivar 86240211
US11044877B2 (en) 2019-08-27 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 85262507
US11076554B2 (en) 2019-08-28 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 87272107
US10986797B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 86440139
US10986799B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 81440919
US11096364B2 (en) 2019-08-28 2021-08-24 M.S. Technologies, L.L.C. Soybean cultivar 81111940
US10966398B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 86240546
US10999999B2 (en) 2019-08-28 2021-05-11 M.S. Technologies, L.L.C. Soybean cultivar 83392343
US10966399B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 80532336
US10952401B1 (en) 2019-08-28 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 83292238
US11071274B2 (en) 2019-08-28 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 83050118
US10959391B2 (en) 2019-08-28 2021-03-30 M.S. Technologies, L.L.C. Soybean cultivar 80412336
US11019791B2 (en) 2019-08-28 2021-06-01 M.S. Technologies, L.L.C. Soybean cultivar 89242215
US10966397B2 (en) 2019-08-28 2021-04-06 M.S. Technologies, L.L.C. Soybean cultivar 82151940
US10986798B2 (en) 2019-08-28 2021-04-27 M.S. Technologies, L.L.C. Soybean cultivar 82230919
US11006606B2 (en) 2019-08-28 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 81201100
US10905082B1 (en) 2019-08-29 2021-02-02 M.S. Technologies, L.L.C. Soybean cultivar 82212235
US10980209B2 (en) 2019-08-29 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 87230016
US10939654B1 (en) 2019-08-29 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 81111423
US11076557B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 83381828
US10912276B1 (en) 2019-08-29 2021-02-09 M.S. Technologies, L.L.C. Soybean cultivar 85161716
US11026391B2 (en) 2019-08-29 2021-06-08 M.S. Technologies, L.L.C. Soybean cultivar 82152612
US10939653B1 (en) 2019-08-29 2021-03-09 M.S. Technologies, L.L.C. Soybean cultivar 88432102
US10925245B1 (en) 2019-08-29 2021-02-23 M.S. Technologies, L.L.C. Soybean cultivar 89021021
US10952402B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 81171312
US11134635B2 (en) 2019-08-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 82371519
US11134636B2 (en) 2019-08-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 83222640
US10952404B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 87092440
US11076555B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 84490022
US11140845B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 88041740
US11140846B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 88070907
US11000001B2 (en) 2019-08-29 2021-05-11 M.S. Technologies, L.L.C. Soybean cultivar 83271604
US11076556B2 (en) 2019-08-29 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 83422133
US10980210B2 (en) 2019-08-29 2021-04-20 M.S. Technologies, L.L.C. Soybean cultivar 80202604
US11044879B2 (en) 2019-08-29 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 82352802
US10952405B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 88103440
US11013198B2 (en) 2019-08-29 2021-05-25 M.S. Technologies, L.L.C. Soybean cultivar 84042612
US11044878B2 (en) 2019-08-29 2021-06-29 M.S. Technologies, L.L.C. Soybean cultivar 88031336
US11071275B2 (en) 2019-08-29 2021-07-27 M.S. Technologies, L.L.C. Soybean cultivar 88060022
US10952403B1 (en) 2019-08-29 2021-03-23 M.S. Technologies, L.L.C. Soybean cultivar 80462430
US11140847B2 (en) 2019-08-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 80372223
US11006607B2 (en) 2019-08-29 2021-05-18 M.S. Technologies, L.L.C. Soybean cultivar 80462534
US11147230B2 (en) 2020-02-13 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 91420287
US11076563B1 (en) 2020-02-13 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 94040702
US11202427B2 (en) 2020-02-13 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 94110636
US11076562B1 (en) 2020-02-13 2021-08-03 M.S. Technologies, L.L.C. Soybean cultivar 95130401
US11051479B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 94140580
US11051481B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 93020437
US11116168B2 (en) 2020-02-13 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 95420460
US11147229B2 (en) 2020-02-13 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 98240355
US11140855B2 (en) 2020-02-13 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 90420357
US11051480B1 (en) 2020-02-13 2021-07-06 M.S. Technologies, L.L.C. Soybean cultivar 93230440
US11191238B2 (en) 2020-02-13 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 99240189
US11134640B2 (en) 2020-02-13 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 94220034
US11109557B1 (en) 2020-02-13 2021-09-07 M.S. Technologies, L.L.C. Soybean cultivar 98110162
US11147231B2 (en) 2020-02-21 2021-10-19 M.S. Technologies, L.L.C. Soybean cultivar 97040540
US11140856B2 (en) 2020-02-21 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 93440976
US11109558B1 (en) 2020-02-21 2021-09-07 M.S. Technologies, L.L.C. Soybean cultivar 91220032
US11213001B2 (en) 2020-06-02 2022-01-04 M.S. Technologies, L.L.C. Soybean cultivar 98320614
US11172637B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 96350326
US11172640B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 91230357
US11116169B1 (en) 2020-06-02 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 92050703
US11102951B1 (en) 2020-06-02 2021-08-31 M.S. Technologies, L.L.C. Soybean cultivar 96130264
US11172638B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 97320638
US11202430B1 (en) 2020-06-02 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 93120753
US11140857B1 (en) 2020-06-02 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 93320341
US11122764B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 91210322
US11122765B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 98220804
US11202429B1 (en) 2020-06-02 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 91410746
US11172639B1 (en) 2020-06-02 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 99310382
US11166430B1 (en) 2020-06-02 2021-11-09 M.S. Technologies, L.L.C. Soybean cultivar 99120525
US11122766B1 (en) 2020-06-02 2021-09-21 M.S. Technologies, L.L.C. Soybean cultivar 96140088
US11172641B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 90140287
US11202433B1 (en) 2020-07-14 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 92010858
US11134642B1 (en) 2020-07-14 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 98272614
US11172642B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 99150287
US11116170B1 (en) 2020-07-14 2021-09-14 M.S. Technologies, L.L.C. Soybean cultivar 91250440
US11197452B1 (en) 2020-07-14 2021-12-14 M.S. Technologies, L.L.C. Soybean cultivar 92140814
US11191240B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 91210615
US11202432B1 (en) 2020-07-14 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 91410530
US11140858B1 (en) 2020-07-14 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 91040342
BR112022027035A2 (en) 2020-07-14 2023-04-11 Pioneer Hi Bred Int INSECTICIDAL PROTEINS AND METHODS FOR THE USE OF THEM
US11191242B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 95450804
US11172643B1 (en) 2020-07-14 2021-11-16 M.S. Technologies, L.L.C. Soybean cultivar 94440162
US11191241B1 (en) 2020-07-14 2021-12-07 M.S. Technologies, L.L.C. Soybean cultivar 92220615
US11202435B1 (en) 2020-07-17 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 92040765
US11252914B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 95111047
US11252913B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 97240377
US11252909B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 94120737
US11259490B2 (en) 2020-07-17 2022-03-01 M.S. Technologies, L.L.C. Soybean cultivar 99250287
US11252911B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 91110447
US11224183B1 (en) 2020-07-17 2022-01-18 M.S. Technologies, L.L.C. Soybean cultivar 95040275
US11252910B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 99350737
US11252912B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 90220377
US11202434B1 (en) 2020-07-17 2021-12-21 M.S. Technologies, L.L.C. Soybean cultivar 93140657
US11219184B1 (en) 2020-07-17 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 95250357
US11252908B2 (en) 2020-07-17 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 98310437
US11140860B1 (en) 2020-07-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 96220972
US11229179B1 (en) 2020-07-29 2022-01-25 M.S. Technologies, L.L.C. Soybean cultivar 91410830
US11197453B1 (en) 2020-07-29 2021-12-14 M.S. Technologies, L.L.C. Soybean cultivar 95130716
US11219185B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 97250069
US11140859B1 (en) 2020-07-29 2021-10-12 M.S. Technologies, L.L.C. Soybean cultivar 90120947
US11219186B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 91120809
US11266102B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 92220922
US11266101B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 96310052
US11337395B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 99090148
US11259491B2 (en) 2020-07-29 2022-03-01 M.S. Technologies, L.L.C. Soybean cultivar 80540918
US11252915B1 (en) 2020-07-29 2022-02-22 M.S. Technologies, L.L.C. Soybean cultivar 99040204
US11266103B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 99150754
US11224184B1 (en) 2020-07-29 2022-01-18 M.S. Technologies, L.L.C. Soybean cultivar 93330609
US11337396B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 92312145
US11337397B2 (en) 2020-07-29 2022-05-24 M.S. Technologies, L.L.C. Soybean cultivar 90442929
US11134643B1 (en) 2020-07-29 2021-10-05 M.S. Technologies, L.L.C. Soybean cultivar 93410922
US11266104B2 (en) 2020-07-29 2022-03-08 M.S. Technologies, L.L.C. Soybean cultivar 99030547
US11178837B1 (en) 2020-07-29 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 92230102
US11330782B2 (en) 2020-07-29 2022-05-17 M.S. Technologies, L.L.C. Soybean cultivar 93070018
US11219187B1 (en) 2020-07-29 2022-01-11 M.S. Technologies, L.L.C. Soybean cultivar 94240013
US11178838B1 (en) 2020-07-29 2021-11-23 M.S. Technologies, L.L.C. Soybean cultivar 99262713
US11363790B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C Soybean cultivar 91320747
US11369075B2 (en) 2020-09-25 2022-06-28 M.S. Technologies, L.L.C. Soybean cultivar 90392435
US11363791B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 96060511
US11363789B2 (en) 2020-09-25 2022-06-21 M.S. Technologies, L.L.C. Soybean cultivar 90440910
US11432513B2 (en) 2020-09-25 2022-09-06 M.S. Technologies, L.L.C. Soybean cultivar 99350040
US11412693B2 (en) 2020-09-25 2022-08-16 M.S. Technologies, L.L.C. Soybean cultivar 95240447
JP2023544016A (en) 2020-09-30 2023-10-19 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド Rapid transformation of monocot explants
JP2023544753A (en) 2020-10-13 2023-10-25 キージーン ナムローゼ フェンノートシャップ Modified promoters of parthenogenetic genes
CA3201992A1 (en) 2020-12-21 2022-06-30 Thi Ninh Thuan NGUYEN Brassica napus plants comprising an improved fertility restorer
AU2021409634A1 (en) 2020-12-21 2023-07-06 Monsanto Technology Llc Novel insect inhibitory proteins
UY39585A (en) 2020-12-23 2022-07-29 Monsanto Technology Llc PROTEINS THAT EXHIBIT INSECT INHIBITOR ACTIVITY AGAINST PESTS OF AGRICULTURAL IMPORTANCE OF CROP PLANTS AND SEEDS
US11540481B2 (en) 2020-12-29 2023-01-03 M.S. Technologies, L.L.C. Soybean cultivar 97282440
US11457602B2 (en) 2020-12-29 2022-10-04 M.S. Technologies, L.L.C. Soybean cultivar 97442034
US11445691B2 (en) 2020-12-29 2022-09-20 M.S. Technologies, L.L.C. Soybean cultivar 92170645
US11477962B2 (en) 2020-12-29 2022-10-25 M.S. Technologies, L.L.C. Soybean cultivar 94110617
WO2022146874A1 (en) 2020-12-31 2022-07-07 Monsanto Technology Llc Novel insect inhibitory proteins
AU2022313321A1 (en) 2021-07-23 2024-02-01 BASF Agricultural Solutions Seed US LLC Blackleg resistant plants and methods for the identification of blackleg resistant plants
US11737418B2 (en) 2021-09-07 2023-08-29 M.S. Technologies, L.L.C. Soybean cultivar 00120926
US11696559B2 (en) 2021-09-07 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 08230349
US11930766B2 (en) 2021-09-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 03220758
US11712016B2 (en) 2021-09-07 2023-08-01 M.S. Technologies, L.L.C. Soybean cultivar 05101723
US11737417B2 (en) 2021-09-07 2023-08-29 M.S. Technologies, L.L.C. Soybean cultivar 09020706
US11716948B2 (en) 2021-09-07 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04010758
US11818998B2 (en) 2021-09-07 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 05370116
US11678637B2 (en) 2021-09-07 2023-06-20 M.S. Technologies, L.L.C. Soybean cultivar 03220116
US11825797B2 (en) 2021-09-07 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 03020534
US11653616B2 (en) 2021-09-07 2023-05-23 M.S. Technologies, L.L.C. Soybean cultivar 03310138
US11678638B2 (en) 2021-09-07 2023-06-20 M.S. Technologies, L.L.C. Soybean cultivar 02050116
US11771039B2 (en) 2021-09-08 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 06210302
US11895974B2 (en) 2021-09-08 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 08140308
US11716950B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 02020322
US11716952B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04233715
US11716949B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04130507
US11839191B2 (en) 2021-09-08 2023-12-12 M.S. Technologies, L.L.C. Soybean cultivar 01230720
US11622528B2 (en) 2021-09-08 2023-04-11 M.S. Technologies, L.L.C. Soybean cultivar 01230324
US11647728B2 (en) 2021-09-08 2023-05-16 M.S. Technologies, L.L.C. Soybean cultivar 04420302
US11825799B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 01120432
US11653617B2 (en) 2021-09-08 2023-05-23 M.S. Technologies, L.L.C. Soybean cultivar 08330707
US11707043B2 (en) 2021-09-08 2023-07-25 M.S. Technologies, L.L.C. Soybean cultivar 02330315
US11716951B2 (en) 2021-09-08 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04130322
US11696560B2 (en) 2021-09-08 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 01440925
US11766017B2 (en) 2021-09-08 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 01430308
US11700828B2 (en) 2021-09-08 2023-07-18 M.S. Technologies, L.L.C. Soybean cultivar 00320209
US11690345B2 (en) 2021-09-08 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 09150308
US11825800B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 05020705
US11825798B2 (en) 2021-09-08 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 07030530
US11832575B2 (en) 2021-09-08 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 09230307
US11696562B2 (en) 2021-09-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 06320913
US11766018B2 (en) 2021-09-10 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 08110534
US11712017B2 (en) 2021-09-10 2023-08-01 M.S. Technologies, L.L.C. Soybean cultivar 02270817
US11716953B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 02440012
US11744213B2 (en) 2021-09-10 2023-09-05 M.S. Technologies, L.L.C. Soybean cultivar 05150332
US11785909B2 (en) 2021-09-10 2023-10-17 M.S. Technologies, L.L.C. Soybean cultivar 08080157
US11856915B2 (en) 2021-09-10 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 00350156
US11716955B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 08080534
US11696561B2 (en) 2021-09-10 2023-07-11 M.S. Technologies, L.L.C. Soybean cultivar 03420817
US11744214B2 (en) 2021-09-10 2023-09-05 M.S. Technologies, L.L.C. Soybean cultivar 04040454
US11716954B2 (en) 2021-09-10 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 04110156
US11690346B2 (en) 2021-09-10 2023-07-04 M.S. Technologies, L.L.C. Soybean cultivar 08210041
US11778971B2 (en) 2021-09-10 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 08150118
US11778972B2 (en) 2021-09-15 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 05120629
US11771040B2 (en) 2021-09-15 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 07110300
US11766019B2 (en) 2021-09-15 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 08220628
US11832576B2 (en) 2021-09-15 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 08050515
US11825801B2 (en) 2021-09-15 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 03120254
US11771041B2 (en) 2021-09-15 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 00230222
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US11785910B2 (en) 2021-09-15 2023-10-17 M.S. Technologies, L.L.C. Soybean cultivar 00150230
US11856916B2 (en) 2021-09-15 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 06380605
US11766020B2 (en) 2021-09-15 2023-09-26 M.S. Technologies, L.L.C. Soybean cultivar 01450536
US11895975B2 (en) 2021-09-15 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 09001515
US11856917B2 (en) 2021-09-15 2024-01-02 M.S. Technologies, L.L.C. Soybean cultivar 02220303
US11812713B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 09080611
US11819001B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 02180205
US11812716B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 04110707
US11818999B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 04420349
US11778973B2 (en) 2021-09-22 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 00150108
US11825802B2 (en) 2021-09-22 2023-11-28 M.S. Technologies, L.L.C. Soybean cultivar 05220177
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US11812712B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 02120535
US11819002B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 06140706
US11819000B2 (en) 2021-09-22 2023-11-21 M.S. Technologies, L.L.C. Soybean cultivar 05030038
US11771043B2 (en) 2021-09-22 2023-10-03 M.S. Technologies, L.L.C. Soybean cultivar 06110608
US11812715B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 05150847
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US11716956B2 (en) 2021-09-22 2023-08-08 M.S. Technologies, L.L.C. Soybean cultivar 07090548
US11812717B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 06360802
US11778974B2 (en) 2021-09-22 2023-10-10 M.S. Technologies, L.L.C. Soybean cultivar 07050021
US11812714B2 (en) 2021-09-22 2023-11-14 M.S. Technologies, L.L.C. Soybean cultivar 03330181
WO2023052562A1 (en) 2021-10-01 2023-04-06 Basf Se Wheat plants with an increased yield
US11832579B2 (en) 2021-10-06 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 04120519
US11930772B2 (en) 2021-10-06 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 04220959
US11832580B2 (en) 2021-10-06 2023-12-05 M.S. Technologies, L.L.C. Soybean cultivar 06150159
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US11925165B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 03050606
US11925162B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 01030624
US11903359B2 (en) 2021-10-06 2024-02-20 M.S. Technologies, L.L.C. Soybean cultivar 05010818
US11925164B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 07370900
US11758870B2 (en) 2021-10-06 2023-09-19 M.S. Technologies, L.L.C. Soybean cultivar 09020446
US11925163B2 (en) 2021-10-06 2024-03-12 M.S. Technologies, L.L.C. Soybean cultivar 07160900
US11839194B2 (en) 2021-10-06 2023-12-12 M.S. Technologies, L.L.C. Soybean cultivar 04020201
US11930773B2 (en) 2021-10-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 81150353
US11895977B2 (en) 2021-10-07 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 03220926
US11930775B2 (en) 2021-10-07 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 09160202
US11895976B2 (en) 2021-10-07 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 04370122
US11930774B2 (en) 2021-10-07 2024-03-19 M.S. Technologies. L.L.C. Soybean cultivar 03040515
US11917975B2 (en) 2021-10-07 2024-03-05 M.S. Technologies, L.L.C. Soybean cultivar 05410624
US11882809B2 (en) 2021-10-07 2024-01-30 M.S. Technologies, L.L.C. Soybean cultivar 04110611
US11895978B2 (en) 2021-11-01 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 03140402
US11895979B2 (en) 2021-11-01 2024-02-13 M.S. Technologies, L.L.C. Soybean cultivar 04420512
US11930776B2 (en) 2021-11-09 2024-03-19 M.S. Technologies, L.L.C. Soybean cultivar 07081321
US20230374480A1 (en) 2022-03-01 2023-11-23 BASF Agricultural Solutions Seed US LLC Cas12a nickases

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
EP0189707B1 (en) 1984-12-28 1993-08-25 Plant Genetic Systems N.V. Recombinant dna which can be introduced into plant cells
US5254799A (en) 1985-01-18 1993-10-19 Plant Genetic Systems N.V. Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants
CA1313830C (en) 1985-08-07 1993-02-23 Dilip Maganlal Shah Glyphosate-resistant plants
US4940835A (en) * 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US5073677A (en) 1986-09-26 1991-12-17 Ciba-Geigy Corporation Herbicidal tolerant plants containing rat glutathione S-transferase gene
AU620317B2 (en) * 1986-09-26 1992-02-20 Calgene, Inc. Intracellular directed delivery of expression products
US4801540A (en) 1986-10-17 1989-01-31 Calgene, Inc. PG gene and its use in plants
US5312910A (en) 1987-05-26 1994-05-17 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase
FR2629098B1 (en) * 1988-03-23 1990-08-10 Rhone Poulenc Agrochimie CHEMICAL GENE OF HERBICIDE RESISTANCE
US5258300A (en) 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
US5538878A (en) 1988-08-08 1996-07-23 Calgene, Inc. Superoxide dismutase expression in plants
GB8825402D0 (en) 1988-10-31 1988-11-30 Cambridge Advanced Tech Sulfonamide resistance genes
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
US5530188A (en) 1990-03-02 1996-06-25 Amoco Corporation Beta-carotene biosynthesis in genetically engineered hosts
US5349123A (en) 1990-12-21 1994-09-20 Calgene, Inc. Glycogen biosynthetic enzymes in plants
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
ES2173077T3 (en) 1990-06-25 2002-10-16 Monsanto Technology Llc PLANTS THAT TOLERATE GLYPHOSATE.
FR2673642B1 (en) * 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie CHIMERIC GENE COMPRISING A PROMOTER CAPABLE OF GIVING INCREASED TOLERANCE TO GLYPHOSATE.

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