CA2061636A1 - Chimeric gene for the transformation of plants - Google Patents

Chimeric gene for the transformation of plants

Info

Publication number
CA2061636A1
CA2061636A1 CA002061636A CA2061636A CA2061636A1 CA 2061636 A1 CA2061636 A1 CA 2061636A1 CA 002061636 A CA002061636 A CA 002061636A CA 2061636 A CA2061636 A CA 2061636A CA 2061636 A1 CA2061636 A1 CA 2061636A1
Authority
CA
Canada
Prior art keywords
transit peptide
plants
gene encoding
region
plant gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002061636A
Other languages
French (fr)
Other versions
CA2061636C (en
Inventor
Michel Lebrun
Bernard Leroux
Alain Sailland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer SAS
Original Assignee
Michel Lebrun
Bernard Leroux
Alain Sailland
Rhone-Poulenc Agrochimie
Rhone-Poulenc Agro
Aventis Cropscience S.A.
Bayer Cropscience Sa
Bayer Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9410557&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2061636(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Michel Lebrun, Bernard Leroux, Alain Sailland, Rhone-Poulenc Agrochimie, Rhone-Poulenc Agro, Aventis Cropscience S.A., Bayer Cropscience Sa, Bayer Sas filed Critical Michel Lebrun
Publication of CA2061636A1 publication Critical patent/CA2061636A1/en
Application granted granted Critical
Publication of CA2061636C publication Critical patent/CA2061636C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/08—Fusion polypeptide containing a localisation/targetting motif containing a chloroplast localisation signal

Abstract

Chimeric gene for conferring to plants an increased tolerance to a herbicide having as its target EPSPS is disclosed. It comprises, in the direction of transcription, a promoter region, a transit peptide region, a coding sequence for glyphosate tolerance and a polyadenylation signal region, wherein the transit peptide region comprises, in the direction of translation, at least one transit peptide of a plant gene encoding a plastid-localised enzyme, a partial sequence of the N-terminal mature part of a plant gene encoding a plastid-localised enzyme and then a second transit peptide of a plant gene encoding a plastic-localised enzyme. The invention also provides for the production of glyphosate-tolerant plants.
CA002061636A 1991-03-05 1992-02-21 Chimeric gene for the transformation of plants Expired - Lifetime CA2061636C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR9102872 1991-03-05
FR9102872A FR2673643B1 (en) 1991-03-05 1991-03-05 TRANSIT PEPTIDE FOR THE INSERTION OF A FOREIGN GENE INTO A PLANT GENE AND PLANTS TRANSFORMED USING THIS PEPTIDE.

Publications (2)

Publication Number Publication Date
CA2061636A1 true CA2061636A1 (en) 1992-09-06
CA2061636C CA2061636C (en) 2002-12-24

Family

ID=9410557

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002061636A Expired - Lifetime CA2061636C (en) 1991-03-05 1992-02-21 Chimeric gene for the transformation of plants

Country Status (17)

Country Link
US (3) US5510471A (en)
EP (2) EP0924299B1 (en)
JP (1) JP3257816B2 (en)
KR (1) KR100233191B1 (en)
AT (2) ATE169338T1 (en)
AU (1) AU652610B2 (en)
BR (1) BR9200790A (en)
CA (1) CA2061636C (en)
DE (2) DE69226466T2 (en)
DK (2) DK0924299T3 (en)
ES (2) ES2118802T3 (en)
FR (1) FR2673643B1 (en)
IE (1) IE920690A1 (en)
IL (1) IL101115A (en)
MX (1) MX9200915A (en)
PT (1) PT924299E (en)
ZA (1) ZA921645B (en)

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