CA2036946A1 - Indexing linkers - Google Patents

Indexing linkers

Info

Publication number
CA2036946A1
CA2036946A1 CA002036946A CA2036946A CA2036946A1 CA 2036946 A1 CA2036946 A1 CA 2036946A1 CA 002036946 A CA002036946 A CA 002036946A CA 2036946 A CA2036946 A CA 2036946A CA 2036946 A1 CA2036946 A1 CA 2036946A1
Authority
CA
Canada
Prior art keywords
fragments
indexing
nucleic acid
linkers
cleavage
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002036946A
Other languages
French (fr)
Other versions
CA2036946C (en
Inventor
Kenneth V. Deugau
Paul Unrau
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Atomic Energy of Canada Ltd AECL
Queens University at Kingston
Original Assignee
Kenneth V. Deugau
Paul Unrau
Queen's University At Kingston
Atomic Energy Of Canada Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kenneth V. Deugau, Paul Unrau, Queen's University At Kingston, Atomic Energy Of Canada Ltd. filed Critical Kenneth V. Deugau
Publication of CA2036946A1 publication Critical patent/CA2036946A1/en
Application granted granted Critical
Publication of CA2036946C publication Critical patent/CA2036946C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Abstract

Synthetic nucleic acid molecules are provided which are called indexing linkers and which can be selectively linked to unknown, or non-identical cohesive ends of nucleic acid fragments.
Such indexing linkers are useful for the selective isolation, identification, amplification, labelling, and modification, of nucleic acid fragments, especially subsets of such fragments released by cleavage using Type IIS restriction endonucleases or restriction endonucleases recognizing interrupted palindromic sequences. Sets of such indexing linker molecules are described which are useful for indexing fragments released by cleavage of a genome. Methods are described for distinguishing such fragments using said indexing linker molecules fox effecting detection, or further modification of such fragments; and for amplifying such fragments by the polymerase chain reaction.
CA002036946A 1990-04-06 1991-02-22 Indexing linkers Expired - Fee Related CA2036946C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50588490A 1990-04-06 1990-04-06
US505,884 1990-04-06

Publications (2)

Publication Number Publication Date
CA2036946A1 true CA2036946A1 (en) 1991-10-07
CA2036946C CA2036946C (en) 2001-10-16

Family

ID=24012286

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002036946A Expired - Fee Related CA2036946C (en) 1990-04-06 1991-02-22 Indexing linkers

Country Status (2)

Country Link
US (2) US5508169A (en)
CA (1) CA2036946C (en)

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