CA2014318A1 - Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay - Google Patents
Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassayInfo
- Publication number
- CA2014318A1 CA2014318A1 CA2014318A CA2014318A CA2014318A1 CA 2014318 A1 CA2014318 A1 CA 2014318A1 CA 2014318 A CA2014318 A CA 2014318A CA 2014318 A CA2014318 A CA 2014318A CA 2014318 A1 CA2014318 A1 CA 2014318A1
- Authority
- CA
- Canada
- Prior art keywords
- heteroatoms
- amphetamine
- tracers
- analyte
- tracer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/946—CNS-stimulants, e.g. cocaine, amphetamines
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/816—Alkaloids, amphetamines, and barbiturates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/901—Drugs of abuse, e.g. narcotics, amphetamine
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/13—Tracers or tags
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
A fluorescent polarization immunoassay(FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents. The tracers have the following general formula:
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/335,627 US5101015A (en) | 1989-04-10 | 1989-04-10 | Reagents for an amphetamine-class fluorescence polarization immunoassay |
US335,627 | 1989-04-10 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2014318A1 true CA2014318A1 (en) | 1990-10-10 |
CA2014318C CA2014318C (en) | 2000-08-08 |
Family
ID=23312578
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002014318A Expired - Fee Related CA2014318C (en) | 1989-04-10 | 1990-04-10 | Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay |
Country Status (6)
Country | Link |
---|---|
US (2) | US5101015A (en) |
EP (1) | EP0399184B1 (en) |
JP (1) | JP2894782B2 (en) |
CA (1) | CA2014318C (en) |
DE (1) | DE69022286T2 (en) |
ES (1) | ES2079390T3 (en) |
Families Citing this family (62)
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US5326708A (en) * | 1992-02-28 | 1994-07-05 | Lewis Douglas E | Forensically acceptable determinations of gestational fetal exposure to drugs and other chemical agents |
CA2096495C (en) * | 1992-06-16 | 2002-07-09 | Kathy Palmer Ordonez | Dual analyte immunoassay |
US5573955A (en) * | 1995-06-05 | 1996-11-12 | Microgenics Corp. | Reducing tyramine interference in immunoassays for amphetamine and methamphetamine |
US6110750A (en) * | 1996-06-28 | 2000-08-29 | Sugden; Edward A. | Rapid detection method of Mycobacterium bovis by fluorescence polarization |
US20030036106A1 (en) * | 1996-10-28 | 2003-02-20 | Burke Thomas J. | Kinase and phosphatase activity measurement using fluorescence polarization |
US5968746A (en) | 1997-11-26 | 1999-10-19 | Schneider; David R. | Method and apparatus for preserving human saliva for testing |
EP1066523A2 (en) * | 1998-03-27 | 2001-01-10 | Microgenics Corporation | Confirmatory assays for small molecule drugs |
US6306616B1 (en) | 1998-03-27 | 2001-10-23 | Microgenics Corporation | Adsorption type confirmatory assays |
WO1999051702A1 (en) * | 1998-04-08 | 1999-10-14 | Terpetschnig Ewald A | Luminescent compounds |
US7411068B2 (en) * | 1998-04-08 | 2008-08-12 | Terpetschnig Ewald A | Luminescent compounds |
US6538129B1 (en) | 1999-04-07 | 2003-03-25 | Ewald A. Terpetschnig | Luminescent compounds |
US7250517B2 (en) * | 1998-04-08 | 2007-07-31 | Ewald A. Terpetschnig | Luminescent compounds |
US20030235846A1 (en) * | 1998-04-08 | 2003-12-25 | Terpetschnig Ewald A. | Luminescent compounds |
AU772807B2 (en) * | 1998-07-01 | 2004-05-06 | Warner-Lambert Company Llc | (-)-pseudoephedrine as a sympathomimetic drug |
GB2361473C (en) * | 2000-03-08 | 2005-06-28 | Microgenics Corp | Ecstasy-class analogs and use of same in detection of ecstasy-class compounds |
US6472228B2 (en) * | 2000-12-04 | 2002-10-29 | Lifepoint, Inc. | Composition and methods for synthesis of novel tracers for detecting amphetamine and methamphetamine in samples |
US6663831B2 (en) | 2001-04-04 | 2003-12-16 | Forefront Diagnostics, Inc. | “One-device” system for testing constituents in fluids |
ATE408843T1 (en) * | 2001-12-20 | 2008-10-15 | Randox Lab Ltd | METHOD AND KIT FOR DETECTION OR DETERMINATION OF 3,4-METHYLENEDIOXYMETHAMPHETAMIN |
US20030171435A1 (en) * | 2002-01-23 | 2003-09-11 | Drug Abuse Sciences, Inc. | New amphetamine derivatives, antibodies against them and pharmaceutical compositions containing them |
US20030170917A1 (en) * | 2002-03-01 | 2003-09-11 | Roche Diagnostics Corporation | Compounds, antibodies, reagent kits, methods of producing antibodies, and methods of detecting analytes |
US7169907B2 (en) * | 2002-03-01 | 2007-01-30 | Roche Diagnostics Operations, Inc. | Derivatives, immunogens, and antibodies for detecting ecstasy-class drugs |
US7101980B2 (en) * | 2002-03-01 | 2006-09-05 | Roche Diagnostics Operations, Inc. | Derivatives, conjugates, and antibodies for detecting ecstasy-class analytes |
US20040087874A1 (en) * | 2002-10-28 | 2004-05-06 | David Schneider | Saliva collection system |
US7619059B2 (en) | 2003-07-29 | 2009-11-17 | Life Technologies Corporation | Bimolecular optical probes |
JP3615752B1 (en) | 2003-11-18 | 2005-02-02 | 淳 高橋 | Small diameter resin twist brush |
US6991911B2 (en) | 2003-12-15 | 2006-01-31 | Dade Behring Inc. | Assay for entactogens |
US7022492B2 (en) | 2003-12-15 | 2006-04-04 | Dade Behring Inc. | Ecstasy haptens and immunogens |
US20050130243A1 (en) * | 2003-12-15 | 2005-06-16 | Zheng Yi F. | Assay for entactogens |
US7115383B2 (en) * | 2004-03-22 | 2006-10-03 | Dade Behring Inc. | Assays for amphetamine and methamphetamine |
US7037669B2 (en) * | 2004-03-22 | 2006-05-02 | Dade Behring Inc. | Assays for amphetamine and methamphetamine using stereospecific reagents |
GB2404022B (en) * | 2004-06-14 | 2005-08-10 | Cozart Bioscience Ltd | Competitive assays for the detection of methamphetamine group drugs |
US20060024768A1 (en) * | 2004-07-29 | 2006-02-02 | Saladax Biomedical, Inc. | Taxol immunoassay |
US20060024769A1 (en) * | 2004-07-29 | 2006-02-02 | Saladax Biomedical Inc. | Taxol immunoassay |
US20060177883A1 (en) * | 2005-02-08 | 2006-08-10 | Saladax Biomedical Inc. | 5-Fluoro-uracil immunoassay |
US20060216767A1 (en) | 2005-03-22 | 2006-09-28 | Saladax Biomedical Inc. | Docetaxel immunoassay |
WO2006104970A1 (en) | 2005-03-30 | 2006-10-05 | Saladax Biomedical Inc. | Doxorubicin immunoassay |
US7189582B2 (en) | 2005-04-27 | 2007-03-13 | Dade Behring Inc. | Compositions and methods for detection of sirolimus |
US7695975B2 (en) | 2005-06-23 | 2010-04-13 | Siemens Healthcare Diagnostics Inc. | Detection of adulterated samples |
US20070281363A1 (en) * | 2006-04-13 | 2007-12-06 | Ewald Terpetschnig | Luminescent compounds |
US20080076188A1 (en) * | 2006-06-19 | 2008-03-27 | Patsenker Leonid D | Luminescent compounds |
EP2129788B1 (en) | 2007-01-30 | 2014-09-03 | SETA BioMedicals, LLC | Luminescent compounds |
US7790401B2 (en) | 2007-08-06 | 2010-09-07 | Siemens Healthcare Diagnostics | Methods for detection of immunosuppressant drugs |
US7910378B2 (en) | 2007-12-14 | 2011-03-22 | Siemens Healthcare Diagnostics Inc. | Methods for detection of hydrophobic drugs |
US8329424B2 (en) | 2010-06-25 | 2012-12-11 | Siemens Healthcare Diagnostics | Reduction in false results in assay measurements |
US10088490B2 (en) | 2010-08-20 | 2018-10-02 | Siemens Healthcare Diagnostics Inc. | Assay for analytes using multiple receptors |
US8426143B2 (en) | 2011-05-24 | 2013-04-23 | Saladax Biomedical Inc. | Gemcitabine immunoassay |
US8771972B2 (en) * | 2011-05-24 | 2014-07-08 | Saladax Biomedical Inc. | Clozapine immunoassay |
EP2742352B1 (en) | 2011-08-12 | 2018-02-28 | Siemens Healthcare Diagnostics Inc. | Detection of sex steroids |
US8771964B2 (en) | 2012-02-02 | 2014-07-08 | Siemens Healthcare Diagnostics Inc. | Compositions and methods for detection of methadone metabolite |
EP2867330B1 (en) | 2012-06-27 | 2018-10-10 | Siemens Healthcare Diagnostics Inc. | Compositions and methods for liquid mixing assessment |
US9244083B2 (en) | 2012-11-30 | 2016-01-26 | Siemens Healthcare Diagnostics Inc. | Compositions and methods for detecting vitamin D |
US9121859B2 (en) | 2012-12-04 | 2015-09-01 | Siemens Healthcare Diagnostics Inc. | Compounds and methods for determination of FKBP-binding immunosuppressant drugs |
US20140308751A1 (en) | 2013-04-12 | 2014-10-16 | Siemens Healthcare Diagnostics Inc. | Assays for Analyte Homologs |
GB201313938D0 (en) | 2013-08-05 | 2013-09-18 | Randox Lab Ltd | Immunoassay for phenethylamines of the 2C and DO sub-families |
US20160252507A1 (en) | 2013-10-24 | 2016-09-01 | Siemens Healthcare Diagnostics Inc. | Assays for macromolecular analytes |
EP3080604B1 (en) | 2013-12-13 | 2019-05-01 | Siemens Healthcare Diagnostics Inc. | Pretreatment agent in non-agglutination assays |
US20160320416A1 (en) | 2013-12-18 | 2016-11-03 | Siemens Healthcare Diagnostics Inc. | Detection of endothelial disease |
GB201409516D0 (en) | 2014-05-29 | 2014-07-16 | Randox Lab Ltd | Immunoassay for compounds of the NBOMe family |
KR20170014013A (en) | 2014-06-27 | 2017-02-07 | 지멘스 헬쓰케어 다이아그노스틱스 인크. | Binding partners specific for vitamin d epimers |
KR20170016009A (en) | 2014-06-27 | 2017-02-10 | 지멘스 헬쓰케어 다이아그노스틱스 인크. | Binding partners specific for vitamin d epimers in vitamin d assays |
US11435342B2 (en) | 2018-04-19 | 2022-09-06 | Ellie LLC | Sample clarification and reduction of background fluorescence for fluorescent detection of analytes |
CN112202628B (en) * | 2020-09-08 | 2022-09-02 | 杭州涂鸦信息技术有限公司 | WiFi module serial port protocol automatic test system and method |
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ATE43662T1 (en) * | 1985-04-30 | 1989-06-15 | Bayer Ag | USE OF BASIC AMIDES TO INCREASE THE PARASITY OF PAPER OR PAPER-LIKE MATERIALS. |
EP0201751A2 (en) * | 1985-05-08 | 1986-11-20 | Abbott Laboratories | 4'-aminomethyfluorescein derivatives for use in fluorescence polarization immunoassys |
EP0218010A3 (en) * | 1985-07-10 | 1988-01-07 | Abbott Laboratories | Ligand detection method and substituted carboxyfluorescein tracers therefor |
US4751190A (en) * | 1985-07-22 | 1988-06-14 | Abbott Laboratories | Fluorescence polarization immunoassay and reagents for use therein |
US4939264A (en) * | 1986-07-14 | 1990-07-03 | Abbott Laboratories | Immunoassay for opiate alkaloids and their metabolites; tracers, immunogens and antibodies |
US4868132A (en) * | 1987-02-03 | 1989-09-19 | Abbott Laboratories | Fluorescence polarization immunoassay for amphetamine/methamphetamine |
NL8700430A (en) * | 1987-02-20 | 1988-09-16 | Texas Instruments Holland | HEATING DEVICE FOR FUEL, IN PARTICULAR DIESEL OIL. |
JPH0737986B2 (en) * | 1988-03-29 | 1995-04-26 | 松下電器産業株式会社 | Immunological detection method |
US5026827A (en) * | 1988-09-02 | 1991-06-25 | Matsushita Electric Industrial Co., Ltd. | Amphetamine-protein complex as immunogen for obtaining antibodies specific to methamphetamine |
-
1989
- 1989-04-10 US US07/335,627 patent/US5101015A/en not_active Expired - Lifetime
-
1990
- 1990-04-03 EP EP90106319A patent/EP0399184B1/en not_active Expired - Lifetime
- 1990-04-03 DE DE69022286T patent/DE69022286T2/en not_active Expired - Fee Related
- 1990-04-03 ES ES90106319T patent/ES2079390T3/en not_active Expired - Lifetime
- 1990-04-09 JP JP2093823A patent/JP2894782B2/en not_active Expired - Fee Related
- 1990-04-10 CA CA002014318A patent/CA2014318C/en not_active Expired - Fee Related
-
1993
- 1993-06-29 US US08/083,928 patent/US5354693A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
ES2079390T3 (en) | 1996-01-16 |
EP0399184A2 (en) | 1990-11-28 |
US5101015A (en) | 1992-03-31 |
JP2894782B2 (en) | 1999-05-24 |
DE69022286T2 (en) | 1996-04-18 |
CA2014318C (en) | 2000-08-08 |
EP0399184A3 (en) | 1991-12-27 |
DE69022286D1 (en) | 1995-10-19 |
EP0399184B1 (en) | 1995-09-13 |
US5354693A (en) | 1994-10-11 |
JPH02300663A (en) | 1990-12-12 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |