CA2011654A1 - Recombinant poxvirus host range selection system - Google Patents

Recombinant poxvirus host range selection system

Info

Publication number
CA2011654A1
CA2011654A1 CA2011654A CA2011654A CA2011654A1 CA 2011654 A1 CA2011654 A1 CA 2011654A1 CA 2011654 A CA2011654 A CA 2011654A CA 2011654 A CA2011654 A CA 2011654A CA 2011654 A1 CA2011654 A1 CA 2011654A1
Authority
CA
Canada
Prior art keywords
host
virus
recombinant
selection system
deletion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2011654A
Other languages
French (fr)
Other versions
CA2011654C (en
Inventor
Enzo Paoletti
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Health Research Inc
Original Assignee
Enzo Paoletti
Health Research Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/320,471 external-priority patent/US5155020A/en
Application filed by Enzo Paoletti, Health Research Incorporated filed Critical Enzo Paoletti
Publication of CA2011654A1 publication Critical patent/CA2011654A1/en
Application granted granted Critical
Publication of CA2011654C publication Critical patent/CA2011654C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16722—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/20011—Rhabdoviridae
    • C12N2760/20111—Lyssavirus, e.g. rabies virus
    • C12N2760/20122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses.
A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MRC-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MRC-5 cells.
CA002011654A 1989-03-08 1990-03-07 Recombinant poxvirus host range selection system Expired - Fee Related CA2011654C (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/320,471 US5155020A (en) 1989-03-08 1989-03-08 Recombinant poxvirus host range selection system
US47817990A 1990-02-14 1990-02-14
US320,471 1990-02-14
USC.I.P.478,179 1990-02-14

Publications (2)

Publication Number Publication Date
CA2011654A1 true CA2011654A1 (en) 1990-09-08
CA2011654C CA2011654C (en) 1995-12-19

Family

ID=26982509

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002011654A Expired - Fee Related CA2011654C (en) 1989-03-08 1990-03-07 Recombinant poxvirus host range selection system

Country Status (15)

Country Link
US (1) US5453364A (en)
JP (1) JP3044062B2 (en)
AT (1) AT400955B (en)
AU (1) AU625584B2 (en)
BE (1) BE1004212A5 (en)
CA (1) CA2011654C (en)
CH (1) CH682669A5 (en)
DE (1) DE4090351T1 (en)
DK (1) DK157091A (en)
GB (1) GB2245272B (en)
IE (1) IE62441B1 (en)
IT (1) IT1239987B (en)
LU (1) LU88000A1 (en)
NL (1) NL9020389A (en)
WO (1) WO1990010693A1 (en)

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KR101710981B1 (en) 2006-07-28 2017-02-28 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Improved vaccines and methods for using the same
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Also Published As

Publication number Publication date
DE4090351T1 (en) 1997-07-24
IE900814L (en) 1990-09-08
LU88000A1 (en) 1992-03-11
GB2245272B (en) 1993-03-17
ATA902490A (en) 1995-09-15
DK157091A (en) 1991-09-26
AU5332090A (en) 1990-10-09
AU625584B2 (en) 1992-07-16
AT400955B (en) 1996-05-28
NL9020389A (en) 1991-12-02
BE1004212A5 (en) 1992-10-13
IT9019623A0 (en) 1990-03-08
JPH04503904A (en) 1992-07-16
IE62441B1 (en) 1995-02-08
WO1990010693A1 (en) 1990-09-20
CA2011654C (en) 1995-12-19
IT1239987B (en) 1993-11-27
US5453364A (en) 1995-09-26
DK157091D0 (en) 1991-09-06
IT9019623A1 (en) 1991-09-08
GB2245272A (en) 1992-01-02
CH682669A5 (en) 1993-10-29
GB9117996D0 (en) 1991-10-09
JP3044062B2 (en) 2000-05-22

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