CA2004068A1 - Hair analysis method - Google Patents

Hair analysis method

Info

Publication number
CA2004068A1
CA2004068A1 CA 2004068 CA2004068A CA2004068A1 CA 2004068 A1 CA2004068 A1 CA 2004068A1 CA 2004068 CA2004068 CA 2004068 CA 2004068 A CA2004068 A CA 2004068A CA 2004068 A1 CA2004068 A1 CA 2004068A1
Authority
CA
Canada
Prior art keywords
sample
keratin
enzyme
mixture
proteinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2004068
Other languages
French (fr)
Other versions
CA2004068C (en
Inventor
Werner A. Baumgartner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Psychemedics Corp
Original Assignee
Werner A. Baumgartner
Psychemedics Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/285,123 external-priority patent/US5324642A/en
Application filed by Werner A. Baumgartner, Psychemedics Corporation filed Critical Werner A. Baumgartner
Publication of CA2004068A1 publication Critical patent/CA2004068A1/en
Application granted granted Critical
Publication of CA2004068C publication Critical patent/CA2004068C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for the direct analysis of analyte in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing a low redox potential compound such as dithiothreitol or dithioerythritol, an enzyme suitable FOR
the degradation of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
CA 2004068 1988-12-16 1989-11-28 Hair analysis method Expired - Lifetime CA2004068C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/285,123 US5324642A (en) 1987-12-28 1988-12-16 Ligand assays of enzymatic hair digests
US285,123 1988-12-16

Publications (2)

Publication Number Publication Date
CA2004068A1 true CA2004068A1 (en) 1990-06-16
CA2004068C CA2004068C (en) 2001-06-19

Family

ID=23092819

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2004068 Expired - Lifetime CA2004068C (en) 1988-12-16 1989-11-28 Hair analysis method

Country Status (1)

Country Link
CA (1) CA2004068C (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0458594A2 (en) * 1990-05-22 1991-11-27 Psychemedics Corporation Analysis of hair
EP0555440A1 (en) * 1991-07-30 1993-08-18 Psychemedics Corp Hair analysis method.
WO1994018561A1 (en) * 1993-02-03 1994-08-18 Psychemedics Corporation Hair analysis method
US5981204A (en) * 1997-05-06 1999-11-09 Johnson; Ted Donald Method for forensically screening hair samples for the presence of cannabinoids

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466579A (en) * 1987-12-28 1995-11-14 Psychemedics Corporation Hair analysis method
US6949344B1 (en) * 1987-12-28 2005-09-27 Psychemical Corporation Hair analysis method
EP0458594A2 (en) * 1990-05-22 1991-11-27 Psychemedics Corporation Analysis of hair
EP0458594A3 (en) * 1990-05-22 1992-10-07 Psychemedics Corporation Analysis of hair
EP0555440A1 (en) * 1991-07-30 1993-08-18 Psychemedics Corp Hair analysis method.
EP0555440A4 (en) * 1991-07-30 1994-03-23 Psychemedics Corporation
WO1994018561A1 (en) * 1993-02-03 1994-08-18 Psychemedics Corporation Hair analysis method
US5981204A (en) * 1997-05-06 1999-11-09 Johnson; Ted Donald Method for forensically screening hair samples for the presence of cannabinoids

Also Published As

Publication number Publication date
CA2004068C (en) 2001-06-19

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