CA2004068A1 - Hair analysis method - Google Patents
Hair analysis methodInfo
- Publication number
- CA2004068A1 CA2004068A1 CA 2004068 CA2004068A CA2004068A1 CA 2004068 A1 CA2004068 A1 CA 2004068A1 CA 2004068 CA2004068 CA 2004068 CA 2004068 A CA2004068 A CA 2004068A CA 2004068 A1 CA2004068 A1 CA 2004068A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- keratin
- enzyme
- mixture
- proteinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A method for the direct analysis of analyte in keratinized structures, e.g., hair, fingernails and toenails, which comprises preparing a mixture containing a low redox potential compound such as dithiothreitol or dithioerythritol, an enzyme suitable FOR
the degradation of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
the degradation of the keratin structure and a sample of the keratin structure; permitting the enzyme to at least substantially degrade the sample of keratin structure, and subjecting the mixture to analysis to determine the identity and amount of analyte in the keratin substance sample. To accelerate the method, cupric sulfate or sodium arsenite may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/285,123 US5324642A (en) | 1987-12-28 | 1988-12-16 | Ligand assays of enzymatic hair digests |
US285,123 | 1988-12-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2004068A1 true CA2004068A1 (en) | 1990-06-16 |
CA2004068C CA2004068C (en) | 2001-06-19 |
Family
ID=23092819
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2004068 Expired - Lifetime CA2004068C (en) | 1988-12-16 | 1989-11-28 | Hair analysis method |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2004068C (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
EP0555440A1 (en) * | 1991-07-30 | 1993-08-18 | Psychemedics Corp | Hair analysis method. |
WO1994018561A1 (en) * | 1993-02-03 | 1994-08-18 | Psychemedics Corporation | Hair analysis method |
US5981204A (en) * | 1997-05-06 | 1999-11-09 | Johnson; Ted Donald | Method for forensically screening hair samples for the presence of cannabinoids |
-
1989
- 1989-11-28 CA CA 2004068 patent/CA2004068C/en not_active Expired - Lifetime
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5466579A (en) * | 1987-12-28 | 1995-11-14 | Psychemedics Corporation | Hair analysis method |
US6949344B1 (en) * | 1987-12-28 | 2005-09-27 | Psychemical Corporation | Hair analysis method |
EP0458594A2 (en) * | 1990-05-22 | 1991-11-27 | Psychemedics Corporation | Analysis of hair |
EP0458594A3 (en) * | 1990-05-22 | 1992-10-07 | Psychemedics Corporation | Analysis of hair |
EP0555440A1 (en) * | 1991-07-30 | 1993-08-18 | Psychemedics Corp | Hair analysis method. |
EP0555440A4 (en) * | 1991-07-30 | 1994-03-23 | Psychemedics Corporation | |
WO1994018561A1 (en) * | 1993-02-03 | 1994-08-18 | Psychemedics Corporation | Hair analysis method |
US5981204A (en) * | 1997-05-06 | 1999-11-09 | Johnson; Ted Donald | Method for forensically screening hair samples for the presence of cannabinoids |
Also Published As
Publication number | Publication date |
---|---|
CA2004068C (en) | 2001-06-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |