CA1337764C - 1,4-dihydropyridine derivatives and pharmaceutical composition thereof - Google Patents
1,4-dihydropyridine derivatives and pharmaceutical composition thereofInfo
- Publication number
- CA1337764C CA1337764C CA000561305A CA561305A CA1337764C CA 1337764 C CA1337764 C CA 1337764C CA 000561305 A CA000561305 A CA 000561305A CA 561305 A CA561305 A CA 561305A CA 1337764 C CA1337764 C CA 1337764C
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- Prior art keywords
- dimethyl
- compound
- alkyl
- piperazinyl
- nitrophenyl
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
- C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
- C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
- C07D211/04—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D211/80—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members
- C07D211/84—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms, with at the most one bond to halogen directly attached to ring carbon atoms
- C07D211/90—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
Abstract
Disclosed are 1,4-dihydropyridine derivatives of the general formula:
(wherein X1 and X2 independently represent hydrogen, fluoromethyl, fluoromethoxy, halogen, cyano, or nitro; R1 represents a lower alkyl; R2 represents acyl, alkoxycarbonyl, acylalkyl, an N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, alkoxycarbonylalkyl, acyloxyalkyl, nitratoalkyl, cyanoalkyl, heterocycle-alkyl, haloalkyl, alkenyl, or alkynyl; A represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 5 carbon atoms; m represents the integer 1, 2 or 3 and acid addition salts thereof; a method of production thereof;
and pharmaceutical compositions containing such compounds. The compounds have pharmacological activities, in particular potent and long lasting hypotensive activity, coronary vasodilatation, cerebral vasodilatation, peripheral vasodilatation, renal vasodilatation and platelet aggregation inhibitory activity.
(wherein X1 and X2 independently represent hydrogen, fluoromethyl, fluoromethoxy, halogen, cyano, or nitro; R1 represents a lower alkyl; R2 represents acyl, alkoxycarbonyl, acylalkyl, an N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, alkoxycarbonylalkyl, acyloxyalkyl, nitratoalkyl, cyanoalkyl, heterocycle-alkyl, haloalkyl, alkenyl, or alkynyl; A represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 5 carbon atoms; m represents the integer 1, 2 or 3 and acid addition salts thereof; a method of production thereof;
and pharmaceutical compositions containing such compounds. The compounds have pharmacological activities, in particular potent and long lasting hypotensive activity, coronary vasodilatation, cerebral vasodilatation, peripheral vasodilatation, renal vasodilatation and platelet aggregation inhibitory activity.
Description
SPECIFICATION
1,4-Dihydropyridine Derivatives and Pharmaceutical Composition Thereof Field of-the Invention The present invention relates to new 1,4-dihydropyridine derivatives which are useful as` pharmaceuticals and acid additlon salts thereof.
Background of the Invention It is known that some 1,4-dihydropyridine compounds possess coronary artery dilating activity and/or vasodepressor activity.
In fact, however, it is difficult to say that creations and pharmaceutical studies of such 1,4-dihydropyridine derivatives have given satisfactory results.
The present inventors made studies with the aim of developing new 1,4-dihydropyridine derivatives which possess very excellent pharmacological activities, in particular potent and long lasting hypotensive activity, coronary vasodilatation, cerebral vasodilatation, peripheral vasodilatation, renal vasodilatation and platelet aggregation inhibitory activity and which are of low toxicity; as a result, the inventors discovered new compounds which possess these activities and which are of low toxicity, and completed the present invention.
Summary of the Invention The present invention relates to 1,4-dihydropyridine derivatives represented by the general formula:
~Xl ~xa ( I ) R'O~C ~\, CO.--~--N ~ R~
H3 C ~\N~ C ~ (c~ )m wherein X and X independently represent hydrogen, fluoromethyl, fluoromethoxy, halogen, cyano, or nitro; R represents a lower alkyl; R represents acyl, alkoxycarbonyl, acylalkyl, an N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, alkoxycarbonylalkyl, acyloxyalkyl, nitratoalkyl, cyanoalkyl, heterocycle-alkyl, haloalkyl, alkenyl, or alkynyl; A represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 5 carbon atoms; m represents the integer 1, 2 or 3, [hereinafter also referred to as Compound (I)] or to acid addition salts thereof Detailed Description of the Invention In the present specification, C means that the number of carbon is x; for example, C1 4 means that the number of carbon is 1 to 4.
The substituents represented by X1 and by x2 in this specification are independently hydrogen, fluoromethyl (e.g.
difluoromethyl, trifluoromethyl), fluoromethoxy (e.g. monofluoro-methoxy, difluoromethoxy), halogen (e.g. chlorine atom, bromine atom, fluorine atom), cyano or nitro.
The lower alkyl represented by R in this specification may be linear, may be branched or may be cyclic, and lower alkyls hav-ing 1 to 4 carbons, such as methyl, ethyl, _-propyl, isopropyl, _-butyl, isobutyl, sec-butyl and cyclopropylmethyl are preferred.
The acyl represented by R in this specification may be an aliphatic acyl, may be an aromatic acyl, or may be a heterocyclic acyl. The aliphatic acyl may be linear, or may be branched. The preferable number of carbons is 1 to 5. When the aliphatic acyl is unsaturated, it is preferable that the aliphatic acyl has 1 to 2 double or triple bonds. The aromatic acyl or heterocyclic acyl may be any of those in which a carbonyl group is bonded dir-ectly to an aromatic group or heterocyclic group and those in which a carbonyl group is bonded to an aromatic group or hetero-cyclic group via aliphatic group (e.g. a saturated or unsaturated aliphatic group having 1 to 3 carbons, and, when it is unsaturated, having 1 to 2 double or triple bonds). Such acyl groups may be repr~q~e~ by R2 -(s)n---co- in which R2 is an aromatic or hetero-cyclic group, B is a saturated or unsaturated aliphatic group hav-ing 1 to 3 carbon atoms and n is 0 or 1. Phenyl and naphthyl are preferred aromatic groups. For the heterocycle, 5- or 6-membered heterocycles, specifically those whose heteroatom is a nitrogen atom or an oxygen atom are preferred. Pyridyl, furyl and piper-idinyl are preferred heterocycles. The aliphatic group, aromatic group and hetero group in the aliphatic acyl, aromatic acyl and heterocyclic acyl may be substituted by halogen (chlorine atom, bromine atom, etc.), hydroxyl group, carboxyl group, lower alkoxy group, acyl (preferably lower alkanoyl) group, acylamino (prefer-ably lower alkanoylamino) group e~c., and the acyl in the sub-stituent acyl group or acylamino group is exem-~ 3 3 f 7 ~ ~ 27103-25 plified by the same acyls as those mentioned above.
As specific examples of appropriate acyls represented by R , mention may be made of formyl, acetyl, crotonoyl, acryloyl, pro-pioloyl, benzoyl, phenylacetyl, cinnamoyl, _-acetaminobenzoyl, _-methoxybenzoyl, _-dimethylaminobenzoyl, _-hydroxycinnamoyl, _-acetaminobenzoyl, furoyl, nicotinoyl, and piperidinomethyl-carbonyl.
The alkoxycarbonyl may be any alkoxycarbonyl having a linear or branched Cl 5 alkoxy, and as specific examples of appropriate alkoxycarbonyls, mention may be made of methoxycarbonyl, ethoxy-carbonyl, propoxycarbonyl, and tert-butoxycarbonyl.
The acyl for the acylalkyl is exemplified by the acyls men-tioned above, and the alkyl for the acylalkyl is exemplified by linear or branched Cl 5 alkyls. As specific examples of appro-priate acyls and alkyls, mention may be made of phenacyl, acetonyl, methylcarbonylethyl, and pyrrolidinocarbonylmethyl.
The N-alkyl-substituted carbamoylalkyl may be monosubstituted, or may be disubstituted. Both alkyls are independently exempli-fied by linear or branched Cl 5 alkyls. As specific examples of appropriate N-alkyl-substituted carbamoylalkyls, mention may be made of methylcarbamoylmethyl, piperazinocarbonylmethyl~ and di-methylcarbamoylmethyl.
The alkoxy and alkyl for the alkoxyalkyl are respectively exemplified by linear or branched Cl 5 alkoxys and Cl 5 alkyls.
As specific examples of appropriate alkoxys and alkyls,mention may be made of methoxyethyl, ethoxyethyl, and methoxypropyl.
The alkoxy and alkyl for the alkoxycarbonylalkyl are respectively exemplified by linear or branched C1 5 alkoxys and C1 5 alkyls. As specific examples of appropriate alkoxycarbonyl-alkyls, mention may be made of methoxycarbonylmethyl, ethoxy-carbonylmethyl, and ethoxycarbonylethyl.
The acyl for the acyloxyalkyl is exemplified by the acyls mentioned above, and the alkyl for the acyloxyalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate acyloxyalkyl, mention may be made of acetoxyethyl and benzoyloxyethyl.
The alkyl for the nitratoalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate nitratoalkyls, mention may be made of nitratoethyl and nitratopropyl.
The alkyl for the cyanoalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate cyanoalkyls, mention may be made of cyanomethyl and cyanoethyl.
For the heterocycl in the heterocycle-alkyl, 5- or 6-membered heterocycles, specifically those whose heteroatom is a nitrogen atom or an oxygen atom are preferred, and specific examples of such heterocycles include piperidino and morpholino. The alkyl for the heterocycle-alkyl is exemplified by linear or branched C1_5 alkyls. As specific examples of appropriate heterocycle-alkyl combinations, mention may be made of piperidinoethyl and morpholinoethyl.
The halogen for the haloalkyl is exemplified by fluorine atom, chlorine atom, bromine atom, etc., and the alkyl for the ~ 3377~4 haloalkYl is exemplified by linear or branched C1 S alkyls.
As specific examples of appropriate haloalkyls, mention may be made of trihalogen-substituted methyls (e.g. trifluoromethyl) and halogen-substituted ethyls (e.g. monofluoroethyl).
The alkenyl and alkynyl are respectively exemplified by linear or branched C2 5 alkenyls and C2 5 alkynyls such as vinyl, propenyl, isopropenyl, butenyl, ethynyl, propynyl, and pentinyl.
The alkylene comprising a carbon atom to which 2 alkyls are bonded and having intotal at least 5 carbon atoms, represented by A in this specification may be linear, or may be branched, and it is preferable that the alkylene be of not more than C10, specifially not more than C8.
The alkylene is specifically exemplified by 2,2-dimethyl-tetramethylene, 2,2-dimethylpentamethylene, 2,2-dimethyl-hexamethylene, 2,2-dimethyltrimethylene, and 1,1-dimethyl-trimethylene. As an example of preferable alkylene, mention may be made of 2,2-dimethyltrimethylene.
As stated above, m represents the integer 1, 2, or 3. It is preferable that m be the integer 1 or 2.
The acid addition salt of Compound (I) of the present invention are not subject to any particular limitation, as long as they are pharmacologically acceptable salts, and they are exemplified by salts of inorganic acids (e.g. hydrochlorides, hydrobromides, phosphates, sulphates) and salts of organic acids (e.g. acetates, succinates, maleates, fumarates, malates, tartrates, methanesulfonates).
The 1,4-dihydropyridine derivative (I) of the present invention and its acid addition salts possess very potent and persistent pharmacological activities. In particular, the 1,4-dihydropyridine derivative (I) represented by the general formula (I) in which R is acylalkyl, N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, cyanoalkyl, heterocyle-alkyl, alkenyl or alkenyl (in particular, R is alkenyl, alkynyl or fluorine-substituted alkyl) or its acid addition salts possess potent and persistent activities, and in addition, are especially excellent in water solubility. Therefore, said derivatives or its acid addition salts can be prepared as drugs which are clinically very easy to handle; for example, they can be formed into eye drops, nasal drops, inhalations, etc., and, in addition, they can be formed into injections of any desired concentration simply by dissolving them in distilled water for injection.
Moreover, even if possessing potent activities, any slightly water-soluble 1,4-dihydropyridine compound is undesirable for therapeutic use because its absorbability varies among patients, while the water-soluble 1,4-dihydropyridine derivative (I) of the present invention is particularly excellent in that it not only provides desirable drug effects but also enables the easier formation of a therapeutic design according to the patients since there is hardly any patient-to-patient variation in its absorption.
As the 1,4-dihydropyridine derivative (I) of the present invention and acid addition salts thereof, there may be mentioned, for example, the following compounds:
~ 337764 *3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropyl meth.yl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-[4-(2-Propenyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-(4-Cyanomethyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(_-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-t4-(2-Methyl-2-propenyl)-1-homopiperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-(4-Allyl-1-homopiperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-t4-(tert-Butyloxycarbonyl)-1-homopiperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate *3-(4-Formyl-1-homopiperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate *2,2-Dimethyl-3-~4-(2-methyl-2-propenyl)-1-piperazinyl~-propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-t4-(2-butenyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-- -dicarboxylate Dihydrochloride *2,2-Dimethyl-3-(4-cinnamyl-1-piperazinyl)propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Dihydrochloride *2,2-Dimethyl-3-C4-(2-butynyl)-1-piperazinyl~propyl Methyl 1l4-Dihydro-2~6-dimethyl-4-(3-nitrophenyl)-3~5-pyridine--dicarboxylate Dihydrochloride *2,2-Dimethyl-3-~4-(4-pentynyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Dihydrochloride *2~2-Dimethyl-3-(4-piperidinoethyl-1-piperazinyl)propyl Methyl 1t4-Dihydro-2~6-dimethyl-4-(3-nitrophenyl)-3l5 pyridinedicarboxylate Trihydrochloride *2,2-Dimethyl-3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-(4-benzoyl-1-piperazinyl)propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Hydrochloride *2,2-Dimethyl-3-C4-(1-methyl-2-oxopropyl)-1-piperazinyl~-propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2~2-Dimethyl-3-~4-(2-cyanoethyl)-1-piperazinyl~pr Methyl 1r4-Dihydro-2/6-dimethyl-4-(3-nitrophenyl)-3~5 pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-~4-(2-furoyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Hydrochloride The 1,4-dihydropyridine derivative (I) of the present invention can be, for example, produced as follows:
Method 1:
The method in which the compound(II)represented by the general formula:
CH3cocHl~ol-A~ R~ (Il) \ (CH~ )~
wherein m, R2 and A have the same definitions as above, the compound(III)represented by the general formula:
N~ ( m) H3C- C = C~CO' R' wherein R1 has the same definition as above, and the compound (IV) repres~nted by the general formula:
~X' l (~) . C~O
wherein X and X have the same definitions as above, are reacted together Said reaction goes on preferably in the presence of an appropriate solvent. Any solvent can be used, as long as it does not interfere with this reaction; solvents which can be used include alkanols such as methanol, ethanol, propanol, isopropanol, butanol and sec-butanol; ethers such as ethyl ether, dioxane, tetrahydrofuran, ethylene glycol monomethyl ether and ethylene glycol dimethyl ether; acetic acid, pyridine, N-N-dimethylfQrmamide, dimethyl sulfoxide , acetonitrile and a mixed solvent thereof.
Reaction temperature is normally about 10 to 150C, preferably about 40 to 120C. In particular, it is preferable that the reaction be carried out at a temperature near to the boiling point of the solvent used. Reaction time is normally 0.5 to 1.5 hours until the completion of the reaction. It is preferable that the compounds(II),(III) and(IV) be used in such amounts that any two of the three compounds are independently present in a ratio of 1 to 1.5 moles thereof to 1 mole of the remaining one compound.
Each starting compound is already known, or can be produced in accordance with a known method [see, for example, J. Am. Chem.
Soc., 67, 1017 (1945)]
The compound (II) can be produced, for example, by the method indicated by the following equations:
Rl-~ N~ Y'-A-OfJ (Vm) ~
(CHl)~n \(CI~J m/
\ Mannich ~ (V~) \ reaction ~ ~ Reduction Diketene R2~ B - C~10 ~
\(C~)m/ Compound (~) (~) wherein y1 represents a group which is reactive wit~ amino group;
B represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 4 carbon atoms; the other symbols have the same definitions as above.
The group reactive with amino group herein represented by yl is exemplified by halogen, p-toluenesulfonate, methanesulfonate, etc., and as preferable halogens, there may be mentioned, for example, a chlorine atom, a bromine atom, and an iodine atom.
The reaction between the compound (V) and the compound(VIII) is preferably carried out in the presence of a base such as sodium hydroxide, calcium hydroxide, sodium carbonate, potassium carbonate, pyridine, or triethylamine, and the reaction can be carried out at 20 to 150C by properly using solvents which do not adversely affect the reaction, for example, organic solvents such as toluene, benzene, methanol, ethanol, dioxane, tetrahydrofuran, acetone, methyl ethyl ketone and N,N-dimethylformamide; and water.
In cases where the halogen represented by Y in the formula (VIII) is a chlorine atom or bromine atom, sodium iodide, potassium iodide, etc. may be present in a ratio of about 0.1 to 2 moles thereof to 1 mole of the compound (V) for promoting the reaction.
The synthesis of the compound(VI) from the compound (V) is carried out by conventional Mannich reaction at 0 to 100C
normally in an appropriate solvent (e.g. methanol, ethanol, isopropanol, acetic acid) using formalin and the corresponding aldehyde as a starting material. The compound (VI)is reduced by a reducing agent such as lithium aluminum hydride or sodium borohydride normally in an appropriate solvent (e.g. methanol, ethanol, isopropanol, ether, tetrahydrofuran) to form the compound(VII). The reaction is normally carried out in the temperature range of from 0C to the boiling point of the solvent used, and will almost complete itself within 24 hours, though reaction time varies according to the method of reduction.
Then, diketene is reacted with the compound(VII) to thereby produce the compound(II). This reaction is normally carried out at a reaction temperature of -10 to 130C, and may be conducted in the presence of a solvent which is inert to the reaction (e.g. methylene chloride, dioxane, tetrahydrofuran, benzene). In addition, for promoting the reaction, a basic catalyst (e.g. pyridine, triethylamine, 4-dimethylaminopyridine) may be used.
Method 2:
The method in which the compound (IX)represented by the general formula:
~X' (~) ~oc~CO~ r ~3 C ~ CH3 wherein Y represents a group which is reactive with amino group;
R1, X1, x2 and A have the same definitions as above, and the compound (V) represented by the general formula:
HN ~1--R (V) \ ( C~1~)7n wherein m and R have the same definitions as above, are reacted together.
The group reactive with amino group represented by y2 in the general formula(IX) is exemplified by the same groups as those mentioned above in relation to y1~
The reaction conditions for this reaction are in accordance with the above-mentioned method 1.
Method 3:
The method in which the compound (X) represented by the general formula:
~X1 R'02 C ~ C00 f~
f~3 C N C H3 wherein R1, X1 and x2 have the same definitlons as above, or its reactive derivatives, and the compound (VII) represented by the general formula:
HO--~--N /~1--R
\(CH ) m/
wherein m, A and R2 have the same definitions as above, are reacted together.
The reactive derivatives of the compound (X) are exemplified by acid halides (e.g. acid chloride, acid bromide) and active esters (e.g. tosylate).
Said reaction is an esterification reaction by carboxylic acid and alcohol, and is carried out by a per se known means.
Method 4:
The method in which the compound(XI) represented by the general formula~
R'O.C "/~C02- A- N ~ -f~ ( X I ) ll ll (CH2)m ~ C '~ /'C~
wherein m, R , X , X , and A have the same definitions as above, and the general formula:
R2_y4 (XII) wherein R has the same definition as above; Y4 represents halogen, tosylate, or the like, are reacted together.
This reaction is preferably carried out in the presence of a base such as sodium hydroxide, calcium hydroxide, sodium carbonate, potassium carbonate, sodium acetate, potasslum acetate, pyridine or triethylamine, and can be carried out at -10 to 130C
by properly using solvents which do not adversely affect the reaction, for example, organic solvents such as toluene, benzene, methanol, ethanol, dioxane, tetrahydrofuran, acetone, chloroform, dichloromethane, methyl ethyl ketone and N,N-dimethylformamide;
and water. In cases where the halogen represented by Y in the formula (XII) is a chlorine atom or bromine atom, sodium iodide, potassium iodide, etc. may be present in a ratio of about 0.1 to 2 moles thereof to 1 mole of the compound (XI) for promoting the reaction. The compound(XI) can be produced by the above-mentioned method, or can be obtained by deprotection from the compound (I) protected by a per se known protective group for amino group (e.g. R2 = formyl, tert-butoxycarbonyl, - 1 33~764 27103-25 methoxycarbonyl, dichloroacetyl, trityl, etc.).
The 1,4-dihydropyridine derivative (I) thus obtained can be collected at any purity by means of per se known methods of sep-aration and purification, for example, concentration, extraction, chromatography, reprecipitation and recrystallization.
An acid addition salts of the 1,4-dihydropyridine deriva-tive (I) can be produced by a per se known method, and said acid addition salts can be converted to the 1,4-dihydropyridine der-ivative (I) in free form by a per se known method.
The 1,4-dihydropyridine derivative (I) of the present in-vention and acid adduct salts thereof exhibit calcium antagon-istic activities such as smooth muscle relaxant activities, e.g., potent and long lasting hypotensive activity, coronary vasodil-atation, cerebral vasodilatation, peripheral vasodilatation, bron-chodilator activity, intraocular smooth muscle relaxant activity, and renal vasodilatation; platelet aggregation inhibitory acti-vity; antiallergic activity and anticancer activity, and, in addition, are of low toxicity.
Therefore, the 1,4-dihyropyridine derivative (I) and acid addition salts thereof are usable for therapeutic and preventive drugs for circulatory diseases such as hypertension, ischemic heart diseases (angina pectoris, myocardiac infarction, etc.,) cerebral and peripheral circulatory disorders (cerebral infarc-tion, transient ischemic attack, renal artery stenosis, etc.,) in mammals such as hymans, bovines, horses, dogs, mice and rats, and for therapeutic and preventive drugs for allergy (asthma), cat-aract, glaucoma, etc.
Namely, 1,4-dihydropyridine derivatives of the present in-vention is a calcium antagonistic agent and useful as antihyper-tensive agent, antianginal agent, cerebral circulation improving agent, peripheral circulation improving agent, renal function im-proving agent, antiarteriosclerosis drug, smooth muscle relaxant, antiallergic drug, therapeutic drug for cataract and therapeutic drug for glaucoma.
17a Moreover, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are for the most part high in water solubility, and thus they are easy to formulate to, for example, eye drops, nasal drops, inhalations, injections, and liniments.
When used as pharmaceutical drugs, for example, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof can be orally or parenterally administered. The form of administration is exemplified by oral drugs such as tablets, capsules and medicated syrups, and by parenteral drugs, for example, liquid injections in solution, emulsion, suspension, etc., and external preparations such as ointments, creams, suppositories, poultices, eye drops, nasal drops, inhalations, liniments and aerosols.
The pharmaceutical preparations in the above-mentioned administration forms can be produced by preparation by a routine method in the presence of additives which are necessary for preparing said preparations, such as conventional carriers, excipients, binders and stabilizers.
Dose and administration frequency can vary according to symptoms, age, body weight, and administration form, but any of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof, when used as a hypotensive drug, for example, can be normally administered to an adult in a dose of about 0.1 to 100 mg, preferably O.S to 50 mg a day in single or several administrations.
The results of pharmacological tests showing the effectiveness of the 1,4-dihydropyridine derivative (I) are hereinafter presented.
Experimental example 1. Hypotensive activitiy [Method] Male spontaneously hypertensive rats at the 12th to 16th week in age and presenting a systolic pressure of around 200 mmHg were used as experimental animals, and 3 to 8 animals were assigned to each group. The subject compound (indicated by the example number), in an aqueous solution or 5% gum arabic suspension, was orally administered in a dose of 10 mg/kg. At the 4th and 8th hours following the administration, the systolic pressure at tail vein of each rat was noninvasively measured by means of a sphygmomanometer (USM-105, Ueda Seisakusho, Japan). A group of rats administered the solvent alone was used as a control group.
[Results] The hypotensive activities (preadministration blood pressure-postadministration blood pressure) of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are shown in Table 1.
As shown in Table 1, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof were potenter and more long lasting in hypotensive activity in comparison with a known dihydropyridine derivative (nicardipine).
Table 1 Hypotensive Activities Example Number Chan~e in blood pressure (mmHg) 4 hours after 8 hours after administration administration Control group - 2 + 1 5~ - 90 - 87 ~ - 56 - 90 Nicardlpine - 29 - 22 Gum arabic or water was administered to rats of the control group.
Experimental example 2. Water solubility test [Method] The subject compounds (indicated by the example number), after being powdered, were placed in water and vigorously shaken at 20+5C for 30 seconds at 5-minute intervals, and within 30 minutes, the amount of water required to dissolve 1 g of the compound (I) of the present invention therein was measured.
[Results] The results showing the solubilities in water of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are presented in Table 2.
Table 2 Solubilities in Water Example number Amount (ml) of water required to dissolve 1 g of the subject compounds .2, - ' 2 1 3377~4 17 3.2 1.6 Nicardipine 160 Nifedipine >10000 Example 1 Synthesis of 3-[4-(2-furoyl)-1-piperazinyl] -2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate hydrochloride 0.73 g of 3-(1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride was dissolved in 5 ml of tetrahydrofuran, and 0.6 ml of triethylamine and 0.13 ml of 2-furoyl chloride were added; the mixture was then stirred for 10 minutes while cooling with ice. After evaporating the solvent, the residue was purified by the chromatography method [20 g silica gel, n-hexane:ethyl acetate (2:5)]to give 0.4 g of an oily substance. This oily substance was dissolved in diethyl ether; a slightly excessive amount of a solution of hydrogen chloride in ethanol was added; the solid which separated was collected by filtration to give 0.42 g of the subject compound (yield: 52~). The IR and NMR data of the compound thus obtained are as follows:
t 33 ~ 7~
I R (Nujol. cm~') 3300, 1695. I620, 1525 ' H - N M R (DMS0-d~, ~ value) 1.05 (6H, s, >C(CH 3) z) 2.26 (3H, s, =C-CI13) 2.35 (3H, s, =C-CH3) 3.57 (3H, s, -C02CH3) 3.95 (2H, s, -C0zCHz-) 5.00 (lH, s, C~-H) 6.60 (lH, dd, J=4Hz, 4l1z, ~ ) 7.05 (lH, d, J=4Hz, ~ ) 7.4 ~ 8.1 (5H, m, phenyl, ~H) 9. 07 (lH, br, > NH) Example 2 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride 1.5 g of 3-(1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride was dissolved in 10 ml of tetrahydrofuran, and 1.2 ml of triethylamine and 0.22 ml of allyl chloride were added. The mixture was then stirred at 50C
for 12 hours. After evaporating the solvent, the residue was ~4 ~d~-~narlc 22 1 33~7~
purified by the chromatography method [20 g silica gel, eluted with ethyl acetate] to give 1.l g of an oily substance. This oily substance was dissolved in diethyl ether; a slightly excessive amount of a solution of hydrogen chloride in ethanol was adde~;
the solid which separated was collected by filtration, after which it was recrystallized from ethanol to thereby give 1.1 g of the subject compound (yield: 75%). The IR and NMR data of *he compound thus obtained are as follows:
I R (Nujol, cm~') 3340, 1690, 1520 H - N M R (DMS0-db, ~ value ) 0.96 (6H, s, >C(CH3) 2) 2. 27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 3. 90 (2H, s, -C0zCHz-) 5.00 (lH, s, C4--H) 5.3 ~6.0 (3H, m, -Cll=CH2) 7.4 ~8.2 (4H, m, phenyl) 9. 20 (111, br, > NH) Example 3 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 1.6 g of ~-nitrobenzaldehyde, 3.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl acetoacetate, and 1.3 g of methyl 3-aminocrotonate we~e dissolved in 10 ml of isopropanol, and the solution was subjected to heating reflux for 12 hours. Then, the solvent was concentrated under reduced pressure. Diethyl ether was added to the residue to give 2.7 g of a colorless powdery crystal (yield: 44%). The IR and.
NMR data of the compound thus obtained are as follows:
I R (Nuiol, cm~') 3300, 1700 ~ 1640, 1520 ' H - ~ M R (CDC13, ~ value) 0.85 (31~, s, > C-Cli3) 0.89 (3H. s, > C- Cll 3) 1.45 (9ll, s~ -C(CI13)3) 2.07 (2H. s, -Cl~z~i<) 2.30 (4H, b r- t, -1~ ~ N-BOC) H ~
2.32 (3H, ss =C-CH3) 2.40 (3H. s =C-CH3)H
3.30 (4H, br-t, -~ ~ N-BOC) 3.67 (3H, s, -COzCH3) 3.82 (2i~, s, -COzCI~z-) 5.41 (lli. s, C~
6.2S (lH. br, > NH) 7.4 ~8.S (4H, m, phenyl) Example 4 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 425 mg of m-nitrobenzaldehyde, 830 mg of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate, and 324 mg of methyl 3-aminocrotonate were dissolved in 6 ml of isopropanol, and the solution was subjected to heating reflux for 12 hours.
Then, the solvent was concentrated under reduced pressure. The residue was purified by the chromatography method [30 g silica gel, eluted with ethyl acetate) to give 1090 mg of the subject compound (yield: 75%). The IR and NMR data of the compound thus obtained are as follows:
I R (Nujol, cm~') 3290, 1700 ~ 1650, 1525 ' H - N M R (CDC13, ~ value) 0.81, 0.85 (6H. s, > C(CH3)z) 2.10 (2H, s, -CHzN<) 2.32, 2.40 (6H, s, =C-CH3) 2.40 (4H, br, -N~ N-C0-) 3.40 (4H, br, - ~ N-C0-) 3.69 (3H, s, -C02CH3) 3.83 (2H, s, -C0zCHz-) 5.11 (lH, s, C~-H) 6.20 (lH, br, >NH) 7.2 ~ 8.2 (4H, m, phenyl) 7.92 (lH, s, >N-CH0) Examples 5 through 28 The compounds of the formula:
NO~
H3CO C~COlCH2--C--Ctl2-N ~1--R
wherein R is any of the groups listed in Table 3 were produced in accordance with the method of Example 1 or Example 2. In the table, -~ means a phenyl group. The IR and NMR data of the compounds thus obtained are as shown in Table 3.
Examples 29 and 30 The compounds of the formula:
~X' ~X2 Cl13 ~
H3COlC~CO~CH2--C--CHl--~N--C~-C~=CI~2 H Dihydrochloride wherein X and x2 independently represent any of the groups listed in Table 4 were produced in accordance with the method of Example 3. The IR and NMR data of the compounds thus obtained are as shown in Table 4.
Table 3 (a) Example R ZIR (cm~') 'H~ N M R Method No. (Nujol) (DMSO-d~, ~ value) 3250 1.00 (6H, s, >C(CH3)2) 2.28 (3H, s, =C-CH3) -COCH2~ 1690 2.36 ~3H, s, =C-CH3) 3.59 (3H, s, -CO2CH3) hydro- 1650 3.75 (2H, s, -COCH2~) 3.92 (2H, s, -COzCH2-) chloride1525 5.00 (lH, s, C4-H) 7.1 ~8.1 (9H, m, phenyl) 9.17 (lH, br, ~NH) 3200 1 00 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 6 -CO ~ 1690 2 35 (3H, s, =C-CH3) 3.57 (3H, s, -COzCH3) hydro- 1640 3.95 (2H, s, -COzCH2-) 5.00 (lH, s, C4-H) ch1Oride1520 7.3~ 8.1 (9H, m, phenyl) 9.18 (lH, br, >NH) 3200 1.05 (611, s, >C(CH3)2) 2.25 (3H, s, =C-CH3) _~
7 -COCH=CH~1690 2.35 (3H, s, =C-CI{3) 3.57 (3H, s, -CO2CH3) 1 hydro- 1650 3.95 (2H, s, -COzCH2-) 5.00 (lH, s, C4-H) _~
chloride1525 7.2 ~8.1 (llH, m, phenyl, -CH=CH-) ~~
9.20 (1ll, br, >NH) Table 3 (b) Example R 2IR (cm~') ' H - N M R Method No. (Nujol) (DMSO-d~, ~ value) 3250 1.05 (6H, s, >C(CH3)z) 2.03 (3H, s, -COCH3) -COCH3 1690 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 8 hydro- 1620 3.60 (3H, s, -COzCHa) 3.95 (2H, s, -COzCH2-) chloride1520 5.00 (lH, s, C4-H) 7.4 ~8.2 (4H, m, phenyl) 9.24 (lH, br, >NH) 3300 1.00 (6H, s, >C(CH3)2) 2.25 (3H, s, =C-CH3) -COCH2Cl1690 2.33 (3H, s, =C-CH3) 2.57 (3H, s, -COzCH3) 9 hydro- 1660 3.90 (2H, s, -COzCH2-) 4.38 (2H, s, -COCH2Cl) 1 chloride1520 5.00 (lH, s, C4-ll) 7.4~8.1 (4H, m, phenyl) 9.15 (lH, br, >NH) w 3200 0.98 (12H, m, >C(CH3)z~ -COCII(CH3)z) w -COCH(CH3) 2 1690 2.28 (3H, s, =C-CH3) 2.38 (3H, s, =C-CH3) _~
hydro- 1650 3.60 (3H, s, -CO2CH3) 3.96 (2H, s, -COzCHz-) 1 chloride 1520 5.00 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9. 23 (lH, br, >NH) Tab1e 3 (c) Example R ZIR (cm~') ' H - N M R Method No. (Nujol) (DMSO-d~, ~ value) H H
0.85 (6H, s, >C(CH3)z) 1.3~ 2.0 (6H, m, -N ~ H) -COCHzN ~ 3200 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 11 dihydro- 1660 3.60 (3H, s, -COzCH3) 3.80 (2H, s, -COzCHz-) 1 chloride 1525 4.27 (2II, s, -COCHzN<) 5.00 (lH, s, C4-H) 7.4~ 8.1 (4H, m, phenyl) 9.35 (lH, br, >NH) 3300 0.97 (6H, s, >C(CH3)2) 2.27 (3H, s, =l-CH3) 12 -CHz-C-CH 1680 2.38 (3H, s, =C-CI13) 3.59 (3H, s, -COzCH3) 2 dihydro- 1520 3.90 (2H, s, -CO2CHz-) 5.00 (lH, s, C4-H) chloride 7.5~ 8.1 (4H, m, phenyl) 9.23 (lH, br, >NH) 0.92 (6H, s, >C(CH3)2) 2.26 (3H, s, =C-CH3) w 13 -CH2CH=CH~ 3400 2.35 (3H, s, =C-CH3) 3.59 (3H, s, -COzCH3) 2 -~
dihydro- 1685 3.85 (2H, s, -COzCHz-) 5.00 (lH, s, C4-H) C~
chloride 1520 7.2~ 8.1 (llH, m, pheny1, -CH=CH-) 9.16 (lH, br, >NII) Table 3 (d) Example RZIR (cm~') ' H - N M R Method No. (Nujol) (DMS0-d~, ~ value) 3350 0.94 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 14 -CHzCHzCN1690 2.35 (3H, s, =C-CH3) 3.58 (3H, s, -COzCH3) 2 dihydro- 1525 3.86 (2H, s, -CO2CHz-) 5.00 (lH, s, C4-H) chloride 7.4 ~8.1 (4H, m, phenyl) 9.18 (lH, br, >NH) 3300 0.94 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) -CIIzCHzOH1690 2.35 (3H, s, =C-CH3) 3.59 (3H, s, -COzCH3) 2 dihydro- 1520 3.86 (2H, s, -C0zCHz-) 5.00 (lH, s, C4-H) chloride 7.4~ 8.1 (4H, m, phenyl) 9.16 (1H, br, >NH) 0.97 (611, s, >C(CH3)z) 1.3~ 2.1 (611, m, -N ~ ~ ) -CHzCHzN ~3350 2.25 (3H, s, =C-CH3) 2.35 (3H, s, =C-CH3)HH
16 trihydro-1690 3.58 (3H, s, -COzCH3) 3.87 (2H, s, -C0zCHz-) 2 chloride1525 5.00 (lH, s, C4-H) -~7.4 ~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) ~, Table 3 (e) Example R ZIR (cm~') ' H - N M R Method No. (Nu;ol) (DMSO-d~, ~ value) 1.00 (6H, m, >C(CH3)2) 1.92 (3H, s, -C(CH3)=CH2) CH33400 2.28 (3H, s, =C-CH3) 2.39 (3H, s, =C-CH3) 17 -CH2C=CHz1690 3.60 (3H, s, -CO2CH3) 3.92 (2H, s, -COzCH2-) 2 dihydro-1525 5.00 (11l, s, C4-H) 5.26 (2H, s, =CHz) chloride 7.4 ~8.1 (4H, m, phenyl) 9.25 (lH, br, >NH) 0.96 (6H, s, >C(CH3)2) 2.26 (3H, s, =C-CHs) -CHzCO~ 3300 2.37 (3H, s, =C-CH3) 3.60 ~3H, s, -CO2CH3) 18 dihydro-1690 3.90 (2H, s, -CO2CHz-) 2 chloride1520 5.00 (3H, br, C 4 -H, > NCHzCO-) Q~
7.4 ~8.2 (9H, m, phenyl) 9.23 (lH, br, >NH) 0.95 (6H, s, >C(CH3)z) -CHCOCH33300 1.42 (3H, d, J=6Hz, -CH(CH3)CO-) 19 CH3 1690 2.25 (6H, s, =C-CH3, -COCH3) 2 iJ~
dihydro-1520 2.35 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) -J
chloride 3.90 (2H, s, -CO2CH2-) 5.00 (lH, br, C4-H) c~
7.4~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) Table 3 (f) Example R Z IR (cm~l) 'H - N M R Method No. (Nujol) (DMS0-d~, ~ value) 0.94 (6H, s, >C(CH3)z) 3350 1.75 (3H, d, J=611z, =C-Cl13) -CHzCH=CHCH3 1690 2.26 (3H, s, =C-CH3) 2.36 (3H, s, =C-CH3) 2dihydro- 1520 3.60 (3H, s, -C02CH3) 3.87 (2H, s, -C0zCHz-) chloride 5.00 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) 0.96 (6H, s, >C(CH3)2) 2.00 (lH, br, -C- CH) -(CH2)3C--CH 3300 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 21 dihydro- 1680 3.60 (3H, s, -COzCH3) 3.88 (2H, s, -C02CH2-) 2 chlorode 1520 5.00 (lH, s, C4-H) 7.4~ 8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) 3200 0.98 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 22 -CHzCHzN~ 0 1690 2.35 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 2 _~
trihydro- 1520 5.00 (lH, s, C4-13) c~
chloride 7.4 ~8.2 (4H, m, phenyl) 9.20 (lH, br, >NH) Table 3 (g) Example R 2IR (cm~l) ' H--N M R Method No. (Nuiol) (DMS0-d b ~ ~ value) 0.98 (6H, s, >C(CH3) z) 2.25 (3H, s, =C-CH3) -CHzCN 3200 2.37 (3H, s, =C-CH3) 3.5$ (3H, s, -COzCH3) 23 dihydro- 1690 3.80 (2H, s, -CHzCN) 3.91 (2H, s, -C0zCHz-) 2 chloride1520 5.00 (lH, s, C4-H) 7.4~8.1 (4H, m, phenyl) 9.25 (lH, br, >NH) 3400 0.99 (611, s, >C(CH3) z) 2.27 (3H, s, =C-CH3) -CHzCHzONOz1690 2.36 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 24 dihydro- 1640 3.90 (2H, s, -C0zCHz-) 2 chloride1520 4.7~5.0 (3H, m, C4-H, -CH20NOz) 7.4~8.1 (411, m, phenyl) 9.20 (lH, br, >NH) 1.05 (6H, s, >C(CH3) 2) 3300 1.26 (3H, t, J=7Hz, -CHzCH3) -CHzC02CzH51690 2.40 (3H, s, =C-CH3) 3.01 (2H, s, >NCHzC-) 2 dihydro- 1520 3.60 (3H, s, -COzCH3) 3.95 (2H, s, -C0zCH
chloride 4.20 (211, s, >NCllze-) 5.00 (lH, s, C4-H) ci~
7.4~8.1 (4H, m, phenyl) 9.34 (lH, br, >NH) Table 3 (h) Example R 2IR (cm~l) lH - N M R Method No. (Nuiol) (DMSO-d~, ~ value) 1.17 (6H, s, >C(CH3) 2) 1.6~ 2.2 (4H, m, -N~H ) 3380 2.30 (3H, s, =C-CH3) 2.40 (3H, s, =C-CH3) 26 -CH2CON~ 1680 2.95 (2H, br, >NCH2C-) 3.61 (3H, s, -CO2CH3) 2 dihydro- 1650 3.99 (2H, s, -C02CH2-) 4.39 (2H, s, >NCH2Ç-) chloride1525 5.02 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9.10 (lH, br, >NH) 1.10 (6H, s, >C(CH3)2) 2.32 (3H, s, =C-CH3) 2.41 (3H, s, =C-CH3) 2.77 (3H, d, J=5Hz, -NHCH3) -CH2CONHCH33400 2.92 (2H, s, >NCHzC-) 3.67 (3H, s, -COzCH3) -27 dihydro- 1680 4.05 (4H, s, -COzCHz-, >NCHzC,-) 2 chloride1520 5.05 (lH, s, C4-H) 7.2 ~ 8.2 (4H, m, phenyl) 8.5 ~8.8 (lH, m, -NHCH3) 0.93 (6H, s, >C(CH3)2) 2.28 (3H, s, =C-CH3) _~
-CH2CH2F 3340 2.38 (3H, s, =C-CH3) 3.60 (3H, s, -CO2CH3) c~
28 dihydro- 1680 3.88 (2H, s, -C02CHz-) 5.01 (lH, s, C4-H) 2 chloride1520 4.50, 5.30 (2H, m, >NCH2CH2CH2F) 7.46~8.20(4H, m, phenyl) 9.20 (lH, br, >NH) Z
V _ ~
V ~ o , ..
I l l V D11 I C~ ,1:~
X : :;
oo o ~ cr~ e-- x C~~0 0 C~4~ 0 Z o ~ ~:: 1 N
S~ 3~ ~ ;e '-- V N _ _ -- V
t~ I O ~ ~ I O ~ E~
A - V ~ ~ ,~ A ~
S m -- X
u~ .. ^ n O Ir --I ~ ~ X m O c~ O C~
-O
, _ . .
X ~
.. . .
_I -O
' . .
-34a-1 33776~
[Formulation Examples of Pharmaceutical Preparations]
Example 1 Formulation example for nasal drops:
Compound of this invention 0.1 mg Physiological saline solution Total 100.0 ml Example 2 Formulation example for eye drops:
Compound of this invention 0.1 mg Sterile solution for eye-drops (pH 5.0) Total 100.0 ml Example 3 Formulation example for injection (aqueous solution):
(in 2 ml ampule) Compound of this invention 1 mg Distilled water for injection Total 2 ml Example 4 Formulation example for injection (solid injection):
(in each vial) Compound of this invention 1 mg Mannitol 100 mg 1 33~764 Example 5 Formulation example for tablets -Compound of this invention 10 mg Lactose 80 mg Magnesium stearate 2 mg Talc 4 mg Add starch to make an entire amount of 120 mg.
According to the above composition, an aqueous solution of starch was added to a premixture of the compound of this invention and lactose, and this was followed by kneading. Then, the resulting mixture was dried and pulverized to thereby give a powder of uniform particles. To this powder a mixture of talc and magnesium stearate was added, and the resulting mixture was tableted to thereby produce the desired tablets.
Example 6 Formulation example for capsules Compound of this invention 5 mg Lactose 100 mg Polyvinylpyrrolidone 3 mg Add stearic acid to make an entire amount of 110 mg.
According to the above composition, polyvinylpyrrolidone and an alcohol solution of stearic acid were added to a premixture of the compound of this invention and lactose, and this was followed by kneading. Then, the resulting mixture was granulated to thereby give granules. The resulting granules, after drying, were filled in No. 4 capsular containers to thereby produce the desired capsules.
Reference example 1 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanal o CH~
H C--C--CH~--N r~l--BOC
c~3 2.9 g of N-(tert-butyloxycarbonyl)piperazine and 1.6 ml of 35% formalin were dissolved in 8 ml of acetic acid, and the resulting solution was stirred at room temperature for 30 minutes.
Then, 1.5 ml of isobutylaldehyde was added, and the resulting mixture was stirred at 50C for 12 hours. After concentration under reduced pressure, the residue was extracted with ethyl acetate and washed with saturated aqueous sodium bicarbonate.
After drying with anhydrous sodium sulfate, the solvent was evaporated under reduced pressure to give 3.9 g of a colorless powdery crystal (yield: 94%). The IR and NMR data of the compound thus obtained are as follows:
1 R (Nujo], cm~') 1720, 1700 N M R (CDC1 3, ~ value) 1.06 (611, s, >C(CH:I)Z) 1.44 (9H, s, -C(CH:~
~ H
2.3 6 ( 4 !1, t, J =S H z, -I`J~ N- l~n C
2.4~ (211, s, -Cl12N < ) 3.34 (411, t, ~=511z, -N ~ 1~l-r'oc) 9.50 (l11, s, -C1~0) 1 33~764 Reference example 2 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropanal O CH~ ~ O
~C-C -CH2- N N - C H
Formylpiperazine as the starting materlal was treated in the same manner as in Reference example 1 to give the subject compound (yield: 98%). The IR and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~') 1715, 1670 H - N M R (CDC13, ~ value) 1.09 (6H, s, >C(CI13)~) 2.50 (2l1, s, -CHzN<) H ~
2 . 4 5 ( /1 Il, m, - C O - N~N - ) 3 . 4 0 ( 'I 11, m, - C O - N N - ) 7.95 (111, br-s, >N-CHO) 9.50 (111, s, -C-CI~O) Reference example 3 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanol CH3 ~
I~OC~J2--C - CH~--1\1 N--BOC
c~3 ~ ~37764 0.14 g of sodium borohydride was dissolved in 4 ml of isopropanol, and 3.8 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanal was added. The solution was stirred at room temperature for 2 hours; then, water was added, and the solution was extracted with methylene chloride. After drying with anhydrous sodium sulfate, the solvent was evaporated under reduced pressure to give 3.8 g of a needle-like crystal (yield: 99%). The IR and NMR data of the compound thus obtained are as follows:
I R (~e~t, cn~ 3450, l70n ' H - N M R (CDCl 3, ~ value) 0.92 (611, s, >C(CI~ 3) 2) 1.45 (9l1, s, -C(CH 3) 3) 2.37 (2l~. s, -Cl3zN<) Hl 2.54 (4H, br-t, -N~-BOC) 3.45 (411, br-t, -N~_~N-BOC)-3.48 (2H, s, -CHzOH) 4.35 (lH, br, -OH) Reference example 4 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropanol ~ 11 HOCH2 - C- CH2 1\1 t\l--CH
3-(4-Formyl-1-piperazinyl)-2,2-dimethylpropanal as the starting material was treated in the same manner as in Reference example 3 to give the subject compound (yield: 85%). The IR
and NMR data of the compound thus obtained are as follows:
I ~ (Neat, cm~') 3450, 1670 N M R (CnC1~, ~ value) 0.93 (~jll, s, >(;(C11 3) z) 2.3~3 (211, s, -CII~N< ) 2. t~O (4111 m, -CO-N N-) 3.40 (411, m, -CO-N N-) 3.45 (21~, s, -CC1~z011) 4.50 (111, br, -Oli) Reference example 5 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl acetoacetate C~
H3 CCOCH C02 CH2 - C - CH2- N ~--BOC
3.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanol and 30 mg of 4-dimethylaminopyridine were dissolved in 10 ml of methylene chloride, and diketene was added while cooling the solution with ice. After stirring the solution for 30 minutes under cooling with ice, water was added and the solution was extracted with methylene chloride. The resulting extract was concentrated under reduced pressure to ~0 ~ ~377~4 give 4.7 g of an oily product (yield: 97%). The IR and NMR
data of the compound thus obtained are as follows:
r~ ;3 ~, (,rll 1 ) l7~(), l72(), l7~0 N M 1~ value) O . 90 (611, s, > C (Cll 3) 2) 1 . 45 (911, ~, - C (Cll 3) 3) 2.18 (211, s, -Cl12N < ) 2 . 26 (3H, s, - COCII 3) 2.45 (411, br-t, -N N-BOC) ( H
3. 39 (411, br- t, -N~N-BOC) 3 . 45 (2H, s, - CûCH 2CO-) 3. 95 (21~, s, -COzCl{2-) Reference example 6 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate CH3 ~ O
H3C~OCH2CO (:~H2 - C- CH~-N r~/- CII
C~3 3-(4-Formyl-1-piperazinyl)-2,2-dimethylpropanol as the starting material was treated in the same manner as in Reference example 5 to give the subject compound (yield: 98%). The IR
and NMR data of the compound thus obtained are as follows:
I 1~ (N(~a~, cm ') 1735, 1710, IG75 '11 - N M R (CDCI~, ~ value) 0.91 (611, s, >C(CII 3) ~) 2.23 (211, s, -Cll 2 N < ) 2.27 (311, m, -COC1~3) 2.51 (411, m, -CO-N~ N-) 3.40 (41~, m, -CO-~__,N-) 3.47 (21~, s, -COCIIzCO-) 3.95 (21~, s, -COZC~12-) 7.96 (111, br-s >NCI!()) Reference example 7 Synthesis of 3~ piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate ~,NO2 \~ CH3 H3CO3C X ~ CO ~ C H 2--C - C H 2 ~N 11 9.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate was dissolved in ethanol and 50 ml of an ethanol solution of 9.9 N hydrogen chloride was added under cooling with ice. After stirring the mixed solution for 1 hour under cooling with ice, isopropyl ether was added.
The solid which separated was collected by filtration, dissolved in saturated aqueous sodium bicarbonate and extracted with ethyl acetate. The solvent was evaporated under reduced pressure and 1 33776~
acetone was added to the residue. The crystal which separated was collected by filtration to give 3.5 g of the subject compound (yield: 43%). The IR and NMR data of the compound thus obtained are as follows:
I R (Nuj ol, cm~ ') 3270, 3220, 1690, l520 ' H - N ~ R (CDC13, ~ value) 0.81 (611, s, >C(C1~3)z) 2.l5 (2~1, s, -C1121~'<) 2.32 (311, s, =C-CI13) 2.40 (311, s, =C,-CI13) 2.70 (411, br-t, --C-N1 N-) H ~ H
3.05 ~411, br - l, C-N N-) ~ H
3. ~(j (311, s, -COzCI~3) .7O (211, s, -(,() 2CII z - ) (111, s, C4-11) 7.2 ~3.3 (611, m, phenyl , > Nll) Reference example 8 Synthesis of 3~ piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate ~ CH3 H3 CO2 C ~ CO 2C H ~--C-- C H ~ ~'I ~ H
H~C CH3 CH3 460 mg of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5_ dicarboxylate was dissolved in 1.5 ml of methanol and 0.3 ml of an ethanol solution of 9.9 N hydrogen chloride was added.
After stirring the mixed solution for one night, isopropyl ether was added. The solid which separated was collected by filtration, dissolved in saturated aqueous sodium bicarbonate and extracted with ethyl acetate. After washing with saturated solution of sodium chloride, the extract was dried with anhydrous sodium sulfate; then, the solvent was evaporated under reduced pressure to give 350 mg of the subject compound (yield: ~31~).
Reference example 9 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropanal O Cl 13 ~
HC - C - C H2 - N ~--C1-12 C~ = Ct~2 CH~
1-Allylpiperazine as the starting material was treated in the same manner as in Reference example 1 to give the subject compound (yield: 75%). The IR and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~') 1720 '~l - N M R (CDC13 ~ value) I.00 (611, s, >C(CI13).) H~l~
H H~H
2. 95 (211, d, J ~ ( l!2 -Cll=) a,. 9 -` 6. 0 (~'311 nl, vinyl) 9. 55 (1 11, i, - (,11()) a,4 Reference example 10 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropanol C~3 f__ H o CH2--C - CH2 - N ~1- C~12 C H = C H~
3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropanal as the starting material was treated in the same manner as in Reference example 3 to give the subject compound (yield: 98%). The IR and NMR data of the compound thus obtained are as follows:
! R (Neat, cm~') 3450 'H -- N M R (CDCI3, ~ value) O. 98 (6H, s, > C (CH3) z) H H
2 . 2 ~ 2 . 7 (lOH, br, - N~N-, - CH 2N < ) 1-l H ~
2. 9S (2H, d, J=6Hz, -Cl12-CII=) 3. 50 (211, s, -CH20H) 3. 70 (111, br, -OH) Reference example 11 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate c~3 /--\
1~3 CCOCH2 C02 C1~2 - C - C H2 - N N--CH~ CH = CH2 \ /
CH~
1 337~64 3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropanol as the starting material was treated in the same manner as in Reference example 5 to give the subject compound (yield: 97%). The IR
and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~ ') 1740, 1720 ' H -- N M R (CDCI3, ~ value) 0.90 (6H, s, >C(CH3)z) 2. 28 (3H, s, -COCH3) H ~H
2. 4 ~2.7 (lOH, br, -N~N-, -CIIzN < ) 3. 00 (2H, d, J=611z, -CH2-CII=) 3. 45 (2H, s, -COCH2CO-) 4. lS (2H, s, -CO2CHz-) 5.0 ~6.0 (3H, m, vinyl)
1,4-Dihydropyridine Derivatives and Pharmaceutical Composition Thereof Field of-the Invention The present invention relates to new 1,4-dihydropyridine derivatives which are useful as` pharmaceuticals and acid additlon salts thereof.
Background of the Invention It is known that some 1,4-dihydropyridine compounds possess coronary artery dilating activity and/or vasodepressor activity.
In fact, however, it is difficult to say that creations and pharmaceutical studies of such 1,4-dihydropyridine derivatives have given satisfactory results.
The present inventors made studies with the aim of developing new 1,4-dihydropyridine derivatives which possess very excellent pharmacological activities, in particular potent and long lasting hypotensive activity, coronary vasodilatation, cerebral vasodilatation, peripheral vasodilatation, renal vasodilatation and platelet aggregation inhibitory activity and which are of low toxicity; as a result, the inventors discovered new compounds which possess these activities and which are of low toxicity, and completed the present invention.
Summary of the Invention The present invention relates to 1,4-dihydropyridine derivatives represented by the general formula:
~Xl ~xa ( I ) R'O~C ~\, CO.--~--N ~ R~
H3 C ~\N~ C ~ (c~ )m wherein X and X independently represent hydrogen, fluoromethyl, fluoromethoxy, halogen, cyano, or nitro; R represents a lower alkyl; R represents acyl, alkoxycarbonyl, acylalkyl, an N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, alkoxycarbonylalkyl, acyloxyalkyl, nitratoalkyl, cyanoalkyl, heterocycle-alkyl, haloalkyl, alkenyl, or alkynyl; A represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 5 carbon atoms; m represents the integer 1, 2 or 3, [hereinafter also referred to as Compound (I)] or to acid addition salts thereof Detailed Description of the Invention In the present specification, C means that the number of carbon is x; for example, C1 4 means that the number of carbon is 1 to 4.
The substituents represented by X1 and by x2 in this specification are independently hydrogen, fluoromethyl (e.g.
difluoromethyl, trifluoromethyl), fluoromethoxy (e.g. monofluoro-methoxy, difluoromethoxy), halogen (e.g. chlorine atom, bromine atom, fluorine atom), cyano or nitro.
The lower alkyl represented by R in this specification may be linear, may be branched or may be cyclic, and lower alkyls hav-ing 1 to 4 carbons, such as methyl, ethyl, _-propyl, isopropyl, _-butyl, isobutyl, sec-butyl and cyclopropylmethyl are preferred.
The acyl represented by R in this specification may be an aliphatic acyl, may be an aromatic acyl, or may be a heterocyclic acyl. The aliphatic acyl may be linear, or may be branched. The preferable number of carbons is 1 to 5. When the aliphatic acyl is unsaturated, it is preferable that the aliphatic acyl has 1 to 2 double or triple bonds. The aromatic acyl or heterocyclic acyl may be any of those in which a carbonyl group is bonded dir-ectly to an aromatic group or heterocyclic group and those in which a carbonyl group is bonded to an aromatic group or hetero-cyclic group via aliphatic group (e.g. a saturated or unsaturated aliphatic group having 1 to 3 carbons, and, when it is unsaturated, having 1 to 2 double or triple bonds). Such acyl groups may be repr~q~e~ by R2 -(s)n---co- in which R2 is an aromatic or hetero-cyclic group, B is a saturated or unsaturated aliphatic group hav-ing 1 to 3 carbon atoms and n is 0 or 1. Phenyl and naphthyl are preferred aromatic groups. For the heterocycle, 5- or 6-membered heterocycles, specifically those whose heteroatom is a nitrogen atom or an oxygen atom are preferred. Pyridyl, furyl and piper-idinyl are preferred heterocycles. The aliphatic group, aromatic group and hetero group in the aliphatic acyl, aromatic acyl and heterocyclic acyl may be substituted by halogen (chlorine atom, bromine atom, etc.), hydroxyl group, carboxyl group, lower alkoxy group, acyl (preferably lower alkanoyl) group, acylamino (prefer-ably lower alkanoylamino) group e~c., and the acyl in the sub-stituent acyl group or acylamino group is exem-~ 3 3 f 7 ~ ~ 27103-25 plified by the same acyls as those mentioned above.
As specific examples of appropriate acyls represented by R , mention may be made of formyl, acetyl, crotonoyl, acryloyl, pro-pioloyl, benzoyl, phenylacetyl, cinnamoyl, _-acetaminobenzoyl, _-methoxybenzoyl, _-dimethylaminobenzoyl, _-hydroxycinnamoyl, _-acetaminobenzoyl, furoyl, nicotinoyl, and piperidinomethyl-carbonyl.
The alkoxycarbonyl may be any alkoxycarbonyl having a linear or branched Cl 5 alkoxy, and as specific examples of appropriate alkoxycarbonyls, mention may be made of methoxycarbonyl, ethoxy-carbonyl, propoxycarbonyl, and tert-butoxycarbonyl.
The acyl for the acylalkyl is exemplified by the acyls men-tioned above, and the alkyl for the acylalkyl is exemplified by linear or branched Cl 5 alkyls. As specific examples of appro-priate acyls and alkyls, mention may be made of phenacyl, acetonyl, methylcarbonylethyl, and pyrrolidinocarbonylmethyl.
The N-alkyl-substituted carbamoylalkyl may be monosubstituted, or may be disubstituted. Both alkyls are independently exempli-fied by linear or branched Cl 5 alkyls. As specific examples of appropriate N-alkyl-substituted carbamoylalkyls, mention may be made of methylcarbamoylmethyl, piperazinocarbonylmethyl~ and di-methylcarbamoylmethyl.
The alkoxy and alkyl for the alkoxyalkyl are respectively exemplified by linear or branched Cl 5 alkoxys and Cl 5 alkyls.
As specific examples of appropriate alkoxys and alkyls,mention may be made of methoxyethyl, ethoxyethyl, and methoxypropyl.
The alkoxy and alkyl for the alkoxycarbonylalkyl are respectively exemplified by linear or branched C1 5 alkoxys and C1 5 alkyls. As specific examples of appropriate alkoxycarbonyl-alkyls, mention may be made of methoxycarbonylmethyl, ethoxy-carbonylmethyl, and ethoxycarbonylethyl.
The acyl for the acyloxyalkyl is exemplified by the acyls mentioned above, and the alkyl for the acyloxyalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate acyloxyalkyl, mention may be made of acetoxyethyl and benzoyloxyethyl.
The alkyl for the nitratoalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate nitratoalkyls, mention may be made of nitratoethyl and nitratopropyl.
The alkyl for the cyanoalkyl is exemplified by linear or branched C1 5 alkyls. As specific examples of appropriate cyanoalkyls, mention may be made of cyanomethyl and cyanoethyl.
For the heterocycl in the heterocycle-alkyl, 5- or 6-membered heterocycles, specifically those whose heteroatom is a nitrogen atom or an oxygen atom are preferred, and specific examples of such heterocycles include piperidino and morpholino. The alkyl for the heterocycle-alkyl is exemplified by linear or branched C1_5 alkyls. As specific examples of appropriate heterocycle-alkyl combinations, mention may be made of piperidinoethyl and morpholinoethyl.
The halogen for the haloalkyl is exemplified by fluorine atom, chlorine atom, bromine atom, etc., and the alkyl for the ~ 3377~4 haloalkYl is exemplified by linear or branched C1 S alkyls.
As specific examples of appropriate haloalkyls, mention may be made of trihalogen-substituted methyls (e.g. trifluoromethyl) and halogen-substituted ethyls (e.g. monofluoroethyl).
The alkenyl and alkynyl are respectively exemplified by linear or branched C2 5 alkenyls and C2 5 alkynyls such as vinyl, propenyl, isopropenyl, butenyl, ethynyl, propynyl, and pentinyl.
The alkylene comprising a carbon atom to which 2 alkyls are bonded and having intotal at least 5 carbon atoms, represented by A in this specification may be linear, or may be branched, and it is preferable that the alkylene be of not more than C10, specifially not more than C8.
The alkylene is specifically exemplified by 2,2-dimethyl-tetramethylene, 2,2-dimethylpentamethylene, 2,2-dimethyl-hexamethylene, 2,2-dimethyltrimethylene, and 1,1-dimethyl-trimethylene. As an example of preferable alkylene, mention may be made of 2,2-dimethyltrimethylene.
As stated above, m represents the integer 1, 2, or 3. It is preferable that m be the integer 1 or 2.
The acid addition salt of Compound (I) of the present invention are not subject to any particular limitation, as long as they are pharmacologically acceptable salts, and they are exemplified by salts of inorganic acids (e.g. hydrochlorides, hydrobromides, phosphates, sulphates) and salts of organic acids (e.g. acetates, succinates, maleates, fumarates, malates, tartrates, methanesulfonates).
The 1,4-dihydropyridine derivative (I) of the present invention and its acid addition salts possess very potent and persistent pharmacological activities. In particular, the 1,4-dihydropyridine derivative (I) represented by the general formula (I) in which R is acylalkyl, N-alkyl-substituted carbamoylalkyl, alkoxyalkyl, cyanoalkyl, heterocyle-alkyl, alkenyl or alkenyl (in particular, R is alkenyl, alkynyl or fluorine-substituted alkyl) or its acid addition salts possess potent and persistent activities, and in addition, are especially excellent in water solubility. Therefore, said derivatives or its acid addition salts can be prepared as drugs which are clinically very easy to handle; for example, they can be formed into eye drops, nasal drops, inhalations, etc., and, in addition, they can be formed into injections of any desired concentration simply by dissolving them in distilled water for injection.
Moreover, even if possessing potent activities, any slightly water-soluble 1,4-dihydropyridine compound is undesirable for therapeutic use because its absorbability varies among patients, while the water-soluble 1,4-dihydropyridine derivative (I) of the present invention is particularly excellent in that it not only provides desirable drug effects but also enables the easier formation of a therapeutic design according to the patients since there is hardly any patient-to-patient variation in its absorption.
As the 1,4-dihydropyridine derivative (I) of the present invention and acid addition salts thereof, there may be mentioned, for example, the following compounds:
~ 337764 *3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropyl meth.yl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-[4-(2-Propenyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-(4-Cyanomethyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(_-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-t4-(2-Methyl-2-propenyl)-1-homopiperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-(4-Allyl-1-homopiperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride *3-t4-(tert-Butyloxycarbonyl)-1-homopiperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate *3-(4-Formyl-1-homopiperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate *2,2-Dimethyl-3-~4-(2-methyl-2-propenyl)-1-piperazinyl~-propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-t4-(2-butenyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-- -dicarboxylate Dihydrochloride *2,2-Dimethyl-3-(4-cinnamyl-1-piperazinyl)propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Dihydrochloride *2,2-Dimethyl-3-C4-(2-butynyl)-1-piperazinyl~propyl Methyl 1l4-Dihydro-2~6-dimethyl-4-(3-nitrophenyl)-3~5-pyridine--dicarboxylate Dihydrochloride *2,2-Dimethyl-3-~4-(4-pentynyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Dihydrochloride *2~2-Dimethyl-3-(4-piperidinoethyl-1-piperazinyl)propyl Methyl 1t4-Dihydro-2~6-dimethyl-4-(3-nitrophenyl)-3l5 pyridinedicarboxylate Trihydrochloride *2,2-Dimethyl-3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-(4-benzoyl-1-piperazinyl)propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Hydrochloride *2,2-Dimethyl-3-C4-(1-methyl-2-oxopropyl)-1-piperazinyl~-propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate Dihydrochloride *2~2-Dimethyl-3-~4-(2-cyanoethyl)-1-piperazinyl~pr Methyl 1r4-Dihydro-2/6-dimethyl-4-(3-nitrophenyl)-3~5 pyridinedicarboxylate Dihydrochloride *2,2-Dimethyl-3-~4-(2-furoyl)-1-piperazinyl~propyl Methyl 1,4-Dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate Hydrochloride The 1,4-dihydropyridine derivative (I) of the present invention can be, for example, produced as follows:
Method 1:
The method in which the compound(II)represented by the general formula:
CH3cocHl~ol-A~ R~ (Il) \ (CH~ )~
wherein m, R2 and A have the same definitions as above, the compound(III)represented by the general formula:
N~ ( m) H3C- C = C~CO' R' wherein R1 has the same definition as above, and the compound (IV) repres~nted by the general formula:
~X' l (~) . C~O
wherein X and X have the same definitions as above, are reacted together Said reaction goes on preferably in the presence of an appropriate solvent. Any solvent can be used, as long as it does not interfere with this reaction; solvents which can be used include alkanols such as methanol, ethanol, propanol, isopropanol, butanol and sec-butanol; ethers such as ethyl ether, dioxane, tetrahydrofuran, ethylene glycol monomethyl ether and ethylene glycol dimethyl ether; acetic acid, pyridine, N-N-dimethylfQrmamide, dimethyl sulfoxide , acetonitrile and a mixed solvent thereof.
Reaction temperature is normally about 10 to 150C, preferably about 40 to 120C. In particular, it is preferable that the reaction be carried out at a temperature near to the boiling point of the solvent used. Reaction time is normally 0.5 to 1.5 hours until the completion of the reaction. It is preferable that the compounds(II),(III) and(IV) be used in such amounts that any two of the three compounds are independently present in a ratio of 1 to 1.5 moles thereof to 1 mole of the remaining one compound.
Each starting compound is already known, or can be produced in accordance with a known method [see, for example, J. Am. Chem.
Soc., 67, 1017 (1945)]
The compound (II) can be produced, for example, by the method indicated by the following equations:
Rl-~ N~ Y'-A-OfJ (Vm) ~
(CHl)~n \(CI~J m/
\ Mannich ~ (V~) \ reaction ~ ~ Reduction Diketene R2~ B - C~10 ~
\(C~)m/ Compound (~) (~) wherein y1 represents a group which is reactive wit~ amino group;
B represents an alkylene comprising a carbon atom to which 2 alkyls are bonded and having in total at least 4 carbon atoms; the other symbols have the same definitions as above.
The group reactive with amino group herein represented by yl is exemplified by halogen, p-toluenesulfonate, methanesulfonate, etc., and as preferable halogens, there may be mentioned, for example, a chlorine atom, a bromine atom, and an iodine atom.
The reaction between the compound (V) and the compound(VIII) is preferably carried out in the presence of a base such as sodium hydroxide, calcium hydroxide, sodium carbonate, potassium carbonate, pyridine, or triethylamine, and the reaction can be carried out at 20 to 150C by properly using solvents which do not adversely affect the reaction, for example, organic solvents such as toluene, benzene, methanol, ethanol, dioxane, tetrahydrofuran, acetone, methyl ethyl ketone and N,N-dimethylformamide; and water.
In cases where the halogen represented by Y in the formula (VIII) is a chlorine atom or bromine atom, sodium iodide, potassium iodide, etc. may be present in a ratio of about 0.1 to 2 moles thereof to 1 mole of the compound (V) for promoting the reaction.
The synthesis of the compound(VI) from the compound (V) is carried out by conventional Mannich reaction at 0 to 100C
normally in an appropriate solvent (e.g. methanol, ethanol, isopropanol, acetic acid) using formalin and the corresponding aldehyde as a starting material. The compound (VI)is reduced by a reducing agent such as lithium aluminum hydride or sodium borohydride normally in an appropriate solvent (e.g. methanol, ethanol, isopropanol, ether, tetrahydrofuran) to form the compound(VII). The reaction is normally carried out in the temperature range of from 0C to the boiling point of the solvent used, and will almost complete itself within 24 hours, though reaction time varies according to the method of reduction.
Then, diketene is reacted with the compound(VII) to thereby produce the compound(II). This reaction is normally carried out at a reaction temperature of -10 to 130C, and may be conducted in the presence of a solvent which is inert to the reaction (e.g. methylene chloride, dioxane, tetrahydrofuran, benzene). In addition, for promoting the reaction, a basic catalyst (e.g. pyridine, triethylamine, 4-dimethylaminopyridine) may be used.
Method 2:
The method in which the compound (IX)represented by the general formula:
~X' (~) ~oc~CO~ r ~3 C ~ CH3 wherein Y represents a group which is reactive with amino group;
R1, X1, x2 and A have the same definitions as above, and the compound (V) represented by the general formula:
HN ~1--R (V) \ ( C~1~)7n wherein m and R have the same definitions as above, are reacted together.
The group reactive with amino group represented by y2 in the general formula(IX) is exemplified by the same groups as those mentioned above in relation to y1~
The reaction conditions for this reaction are in accordance with the above-mentioned method 1.
Method 3:
The method in which the compound (X) represented by the general formula:
~X1 R'02 C ~ C00 f~
f~3 C N C H3 wherein R1, X1 and x2 have the same definitlons as above, or its reactive derivatives, and the compound (VII) represented by the general formula:
HO--~--N /~1--R
\(CH ) m/
wherein m, A and R2 have the same definitions as above, are reacted together.
The reactive derivatives of the compound (X) are exemplified by acid halides (e.g. acid chloride, acid bromide) and active esters (e.g. tosylate).
Said reaction is an esterification reaction by carboxylic acid and alcohol, and is carried out by a per se known means.
Method 4:
The method in which the compound(XI) represented by the general formula~
R'O.C "/~C02- A- N ~ -f~ ( X I ) ll ll (CH2)m ~ C '~ /'C~
wherein m, R , X , X , and A have the same definitions as above, and the general formula:
R2_y4 (XII) wherein R has the same definition as above; Y4 represents halogen, tosylate, or the like, are reacted together.
This reaction is preferably carried out in the presence of a base such as sodium hydroxide, calcium hydroxide, sodium carbonate, potassium carbonate, sodium acetate, potasslum acetate, pyridine or triethylamine, and can be carried out at -10 to 130C
by properly using solvents which do not adversely affect the reaction, for example, organic solvents such as toluene, benzene, methanol, ethanol, dioxane, tetrahydrofuran, acetone, chloroform, dichloromethane, methyl ethyl ketone and N,N-dimethylformamide;
and water. In cases where the halogen represented by Y in the formula (XII) is a chlorine atom or bromine atom, sodium iodide, potassium iodide, etc. may be present in a ratio of about 0.1 to 2 moles thereof to 1 mole of the compound (XI) for promoting the reaction. The compound(XI) can be produced by the above-mentioned method, or can be obtained by deprotection from the compound (I) protected by a per se known protective group for amino group (e.g. R2 = formyl, tert-butoxycarbonyl, - 1 33~764 27103-25 methoxycarbonyl, dichloroacetyl, trityl, etc.).
The 1,4-dihydropyridine derivative (I) thus obtained can be collected at any purity by means of per se known methods of sep-aration and purification, for example, concentration, extraction, chromatography, reprecipitation and recrystallization.
An acid addition salts of the 1,4-dihydropyridine deriva-tive (I) can be produced by a per se known method, and said acid addition salts can be converted to the 1,4-dihydropyridine der-ivative (I) in free form by a per se known method.
The 1,4-dihydropyridine derivative (I) of the present in-vention and acid adduct salts thereof exhibit calcium antagon-istic activities such as smooth muscle relaxant activities, e.g., potent and long lasting hypotensive activity, coronary vasodil-atation, cerebral vasodilatation, peripheral vasodilatation, bron-chodilator activity, intraocular smooth muscle relaxant activity, and renal vasodilatation; platelet aggregation inhibitory acti-vity; antiallergic activity and anticancer activity, and, in addition, are of low toxicity.
Therefore, the 1,4-dihyropyridine derivative (I) and acid addition salts thereof are usable for therapeutic and preventive drugs for circulatory diseases such as hypertension, ischemic heart diseases (angina pectoris, myocardiac infarction, etc.,) cerebral and peripheral circulatory disorders (cerebral infarc-tion, transient ischemic attack, renal artery stenosis, etc.,) in mammals such as hymans, bovines, horses, dogs, mice and rats, and for therapeutic and preventive drugs for allergy (asthma), cat-aract, glaucoma, etc.
Namely, 1,4-dihydropyridine derivatives of the present in-vention is a calcium antagonistic agent and useful as antihyper-tensive agent, antianginal agent, cerebral circulation improving agent, peripheral circulation improving agent, renal function im-proving agent, antiarteriosclerosis drug, smooth muscle relaxant, antiallergic drug, therapeutic drug for cataract and therapeutic drug for glaucoma.
17a Moreover, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are for the most part high in water solubility, and thus they are easy to formulate to, for example, eye drops, nasal drops, inhalations, injections, and liniments.
When used as pharmaceutical drugs, for example, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof can be orally or parenterally administered. The form of administration is exemplified by oral drugs such as tablets, capsules and medicated syrups, and by parenteral drugs, for example, liquid injections in solution, emulsion, suspension, etc., and external preparations such as ointments, creams, suppositories, poultices, eye drops, nasal drops, inhalations, liniments and aerosols.
The pharmaceutical preparations in the above-mentioned administration forms can be produced by preparation by a routine method in the presence of additives which are necessary for preparing said preparations, such as conventional carriers, excipients, binders and stabilizers.
Dose and administration frequency can vary according to symptoms, age, body weight, and administration form, but any of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof, when used as a hypotensive drug, for example, can be normally administered to an adult in a dose of about 0.1 to 100 mg, preferably O.S to 50 mg a day in single or several administrations.
The results of pharmacological tests showing the effectiveness of the 1,4-dihydropyridine derivative (I) are hereinafter presented.
Experimental example 1. Hypotensive activitiy [Method] Male spontaneously hypertensive rats at the 12th to 16th week in age and presenting a systolic pressure of around 200 mmHg were used as experimental animals, and 3 to 8 animals were assigned to each group. The subject compound (indicated by the example number), in an aqueous solution or 5% gum arabic suspension, was orally administered in a dose of 10 mg/kg. At the 4th and 8th hours following the administration, the systolic pressure at tail vein of each rat was noninvasively measured by means of a sphygmomanometer (USM-105, Ueda Seisakusho, Japan). A group of rats administered the solvent alone was used as a control group.
[Results] The hypotensive activities (preadministration blood pressure-postadministration blood pressure) of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are shown in Table 1.
As shown in Table 1, the 1,4-dihydropyridine derivative (I) and acid addition salts thereof were potenter and more long lasting in hypotensive activity in comparison with a known dihydropyridine derivative (nicardipine).
Table 1 Hypotensive Activities Example Number Chan~e in blood pressure (mmHg) 4 hours after 8 hours after administration administration Control group - 2 + 1 5~ - 90 - 87 ~ - 56 - 90 Nicardlpine - 29 - 22 Gum arabic or water was administered to rats of the control group.
Experimental example 2. Water solubility test [Method] The subject compounds (indicated by the example number), after being powdered, were placed in water and vigorously shaken at 20+5C for 30 seconds at 5-minute intervals, and within 30 minutes, the amount of water required to dissolve 1 g of the compound (I) of the present invention therein was measured.
[Results] The results showing the solubilities in water of the 1,4-dihydropyridine derivative (I) and acid addition salts thereof are presented in Table 2.
Table 2 Solubilities in Water Example number Amount (ml) of water required to dissolve 1 g of the subject compounds .2, - ' 2 1 3377~4 17 3.2 1.6 Nicardipine 160 Nifedipine >10000 Example 1 Synthesis of 3-[4-(2-furoyl)-1-piperazinyl] -2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate hydrochloride 0.73 g of 3-(1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride was dissolved in 5 ml of tetrahydrofuran, and 0.6 ml of triethylamine and 0.13 ml of 2-furoyl chloride were added; the mixture was then stirred for 10 minutes while cooling with ice. After evaporating the solvent, the residue was purified by the chromatography method [20 g silica gel, n-hexane:ethyl acetate (2:5)]to give 0.4 g of an oily substance. This oily substance was dissolved in diethyl ether; a slightly excessive amount of a solution of hydrogen chloride in ethanol was added; the solid which separated was collected by filtration to give 0.42 g of the subject compound (yield: 52~). The IR and NMR data of the compound thus obtained are as follows:
t 33 ~ 7~
I R (Nujol. cm~') 3300, 1695. I620, 1525 ' H - N M R (DMS0-d~, ~ value) 1.05 (6H, s, >C(CH 3) z) 2.26 (3H, s, =C-CI13) 2.35 (3H, s, =C-CH3) 3.57 (3H, s, -C02CH3) 3.95 (2H, s, -C0zCHz-) 5.00 (lH, s, C~-H) 6.60 (lH, dd, J=4Hz, 4l1z, ~ ) 7.05 (lH, d, J=4Hz, ~ ) 7.4 ~ 8.1 (5H, m, phenyl, ~H) 9. 07 (lH, br, > NH) Example 2 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride 1.5 g of 3-(1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate dihydrochloride was dissolved in 10 ml of tetrahydrofuran, and 1.2 ml of triethylamine and 0.22 ml of allyl chloride were added. The mixture was then stirred at 50C
for 12 hours. After evaporating the solvent, the residue was ~4 ~d~-~narlc 22 1 33~7~
purified by the chromatography method [20 g silica gel, eluted with ethyl acetate] to give 1.l g of an oily substance. This oily substance was dissolved in diethyl ether; a slightly excessive amount of a solution of hydrogen chloride in ethanol was adde~;
the solid which separated was collected by filtration, after which it was recrystallized from ethanol to thereby give 1.1 g of the subject compound (yield: 75%). The IR and NMR data of *he compound thus obtained are as follows:
I R (Nujol, cm~') 3340, 1690, 1520 H - N M R (DMS0-db, ~ value ) 0.96 (6H, s, >C(CH3) 2) 2. 27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 3. 90 (2H, s, -C0zCHz-) 5.00 (lH, s, C4--H) 5.3 ~6.0 (3H, m, -Cll=CH2) 7.4 ~8.2 (4H, m, phenyl) 9. 20 (111, br, > NH) Example 3 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 1.6 g of ~-nitrobenzaldehyde, 3.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl acetoacetate, and 1.3 g of methyl 3-aminocrotonate we~e dissolved in 10 ml of isopropanol, and the solution was subjected to heating reflux for 12 hours. Then, the solvent was concentrated under reduced pressure. Diethyl ether was added to the residue to give 2.7 g of a colorless powdery crystal (yield: 44%). The IR and.
NMR data of the compound thus obtained are as follows:
I R (Nuiol, cm~') 3300, 1700 ~ 1640, 1520 ' H - ~ M R (CDC13, ~ value) 0.85 (31~, s, > C-Cli3) 0.89 (3H. s, > C- Cll 3) 1.45 (9ll, s~ -C(CI13)3) 2.07 (2H. s, -Cl~z~i<) 2.30 (4H, b r- t, -1~ ~ N-BOC) H ~
2.32 (3H, ss =C-CH3) 2.40 (3H. s =C-CH3)H
3.30 (4H, br-t, -~ ~ N-BOC) 3.67 (3H, s, -COzCH3) 3.82 (2i~, s, -COzCI~z-) 5.41 (lli. s, C~
6.2S (lH. br, > NH) 7.4 ~8.S (4H, m, phenyl) Example 4 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate 425 mg of m-nitrobenzaldehyde, 830 mg of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate, and 324 mg of methyl 3-aminocrotonate were dissolved in 6 ml of isopropanol, and the solution was subjected to heating reflux for 12 hours.
Then, the solvent was concentrated under reduced pressure. The residue was purified by the chromatography method [30 g silica gel, eluted with ethyl acetate) to give 1090 mg of the subject compound (yield: 75%). The IR and NMR data of the compound thus obtained are as follows:
I R (Nujol, cm~') 3290, 1700 ~ 1650, 1525 ' H - N M R (CDC13, ~ value) 0.81, 0.85 (6H. s, > C(CH3)z) 2.10 (2H, s, -CHzN<) 2.32, 2.40 (6H, s, =C-CH3) 2.40 (4H, br, -N~ N-C0-) 3.40 (4H, br, - ~ N-C0-) 3.69 (3H, s, -C02CH3) 3.83 (2H, s, -C0zCHz-) 5.11 (lH, s, C~-H) 6.20 (lH, br, >NH) 7.2 ~ 8.2 (4H, m, phenyl) 7.92 (lH, s, >N-CH0) Examples 5 through 28 The compounds of the formula:
NO~
H3CO C~COlCH2--C--Ctl2-N ~1--R
wherein R is any of the groups listed in Table 3 were produced in accordance with the method of Example 1 or Example 2. In the table, -~ means a phenyl group. The IR and NMR data of the compounds thus obtained are as shown in Table 3.
Examples 29 and 30 The compounds of the formula:
~X' ~X2 Cl13 ~
H3COlC~CO~CH2--C--CHl--~N--C~-C~=CI~2 H Dihydrochloride wherein X and x2 independently represent any of the groups listed in Table 4 were produced in accordance with the method of Example 3. The IR and NMR data of the compounds thus obtained are as shown in Table 4.
Table 3 (a) Example R ZIR (cm~') 'H~ N M R Method No. (Nujol) (DMSO-d~, ~ value) 3250 1.00 (6H, s, >C(CH3)2) 2.28 (3H, s, =C-CH3) -COCH2~ 1690 2.36 ~3H, s, =C-CH3) 3.59 (3H, s, -CO2CH3) hydro- 1650 3.75 (2H, s, -COCH2~) 3.92 (2H, s, -COzCH2-) chloride1525 5.00 (lH, s, C4-H) 7.1 ~8.1 (9H, m, phenyl) 9.17 (lH, br, ~NH) 3200 1 00 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 6 -CO ~ 1690 2 35 (3H, s, =C-CH3) 3.57 (3H, s, -COzCH3) hydro- 1640 3.95 (2H, s, -COzCH2-) 5.00 (lH, s, C4-H) ch1Oride1520 7.3~ 8.1 (9H, m, phenyl) 9.18 (lH, br, >NH) 3200 1.05 (611, s, >C(CH3)2) 2.25 (3H, s, =C-CH3) _~
7 -COCH=CH~1690 2.35 (3H, s, =C-CI{3) 3.57 (3H, s, -CO2CH3) 1 hydro- 1650 3.95 (2H, s, -COzCH2-) 5.00 (lH, s, C4-H) _~
chloride1525 7.2 ~8.1 (llH, m, phenyl, -CH=CH-) ~~
9.20 (1ll, br, >NH) Table 3 (b) Example R 2IR (cm~') ' H - N M R Method No. (Nujol) (DMSO-d~, ~ value) 3250 1.05 (6H, s, >C(CH3)z) 2.03 (3H, s, -COCH3) -COCH3 1690 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 8 hydro- 1620 3.60 (3H, s, -COzCHa) 3.95 (2H, s, -COzCH2-) chloride1520 5.00 (lH, s, C4-H) 7.4 ~8.2 (4H, m, phenyl) 9.24 (lH, br, >NH) 3300 1.00 (6H, s, >C(CH3)2) 2.25 (3H, s, =C-CH3) -COCH2Cl1690 2.33 (3H, s, =C-CH3) 2.57 (3H, s, -COzCH3) 9 hydro- 1660 3.90 (2H, s, -COzCH2-) 4.38 (2H, s, -COCH2Cl) 1 chloride1520 5.00 (lH, s, C4-ll) 7.4~8.1 (4H, m, phenyl) 9.15 (lH, br, >NH) w 3200 0.98 (12H, m, >C(CH3)z~ -COCII(CH3)z) w -COCH(CH3) 2 1690 2.28 (3H, s, =C-CH3) 2.38 (3H, s, =C-CH3) _~
hydro- 1650 3.60 (3H, s, -CO2CH3) 3.96 (2H, s, -COzCHz-) 1 chloride 1520 5.00 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9. 23 (lH, br, >NH) Tab1e 3 (c) Example R ZIR (cm~') ' H - N M R Method No. (Nujol) (DMSO-d~, ~ value) H H
0.85 (6H, s, >C(CH3)z) 1.3~ 2.0 (6H, m, -N ~ H) -COCHzN ~ 3200 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 11 dihydro- 1660 3.60 (3H, s, -COzCH3) 3.80 (2H, s, -COzCHz-) 1 chloride 1525 4.27 (2II, s, -COCHzN<) 5.00 (lH, s, C4-H) 7.4~ 8.1 (4H, m, phenyl) 9.35 (lH, br, >NH) 3300 0.97 (6H, s, >C(CH3)2) 2.27 (3H, s, =l-CH3) 12 -CHz-C-CH 1680 2.38 (3H, s, =C-CI13) 3.59 (3H, s, -COzCH3) 2 dihydro- 1520 3.90 (2H, s, -CO2CHz-) 5.00 (lH, s, C4-H) chloride 7.5~ 8.1 (4H, m, phenyl) 9.23 (lH, br, >NH) 0.92 (6H, s, >C(CH3)2) 2.26 (3H, s, =C-CH3) w 13 -CH2CH=CH~ 3400 2.35 (3H, s, =C-CH3) 3.59 (3H, s, -COzCH3) 2 -~
dihydro- 1685 3.85 (2H, s, -COzCHz-) 5.00 (lH, s, C4-H) C~
chloride 1520 7.2~ 8.1 (llH, m, pheny1, -CH=CH-) 9.16 (lH, br, >NII) Table 3 (d) Example RZIR (cm~') ' H - N M R Method No. (Nujol) (DMS0-d~, ~ value) 3350 0.94 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 14 -CHzCHzCN1690 2.35 (3H, s, =C-CH3) 3.58 (3H, s, -COzCH3) 2 dihydro- 1525 3.86 (2H, s, -CO2CHz-) 5.00 (lH, s, C4-H) chloride 7.4 ~8.1 (4H, m, phenyl) 9.18 (lH, br, >NH) 3300 0.94 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) -CIIzCHzOH1690 2.35 (3H, s, =C-CH3) 3.59 (3H, s, -COzCH3) 2 dihydro- 1520 3.86 (2H, s, -C0zCHz-) 5.00 (lH, s, C4-H) chloride 7.4~ 8.1 (4H, m, phenyl) 9.16 (1H, br, >NH) 0.97 (611, s, >C(CH3)z) 1.3~ 2.1 (611, m, -N ~ ~ ) -CHzCHzN ~3350 2.25 (3H, s, =C-CH3) 2.35 (3H, s, =C-CH3)HH
16 trihydro-1690 3.58 (3H, s, -COzCH3) 3.87 (2H, s, -C0zCHz-) 2 chloride1525 5.00 (lH, s, C4-H) -~7.4 ~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) ~, Table 3 (e) Example R ZIR (cm~') ' H - N M R Method No. (Nu;ol) (DMSO-d~, ~ value) 1.00 (6H, m, >C(CH3)2) 1.92 (3H, s, -C(CH3)=CH2) CH33400 2.28 (3H, s, =C-CH3) 2.39 (3H, s, =C-CH3) 17 -CH2C=CHz1690 3.60 (3H, s, -CO2CH3) 3.92 (2H, s, -COzCH2-) 2 dihydro-1525 5.00 (11l, s, C4-H) 5.26 (2H, s, =CHz) chloride 7.4 ~8.1 (4H, m, phenyl) 9.25 (lH, br, >NH) 0.96 (6H, s, >C(CH3)2) 2.26 (3H, s, =C-CHs) -CHzCO~ 3300 2.37 (3H, s, =C-CH3) 3.60 ~3H, s, -CO2CH3) 18 dihydro-1690 3.90 (2H, s, -CO2CHz-) 2 chloride1520 5.00 (3H, br, C 4 -H, > NCHzCO-) Q~
7.4 ~8.2 (9H, m, phenyl) 9.23 (lH, br, >NH) 0.95 (6H, s, >C(CH3)z) -CHCOCH33300 1.42 (3H, d, J=6Hz, -CH(CH3)CO-) 19 CH3 1690 2.25 (6H, s, =C-CH3, -COCH3) 2 iJ~
dihydro-1520 2.35 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) -J
chloride 3.90 (2H, s, -CO2CH2-) 5.00 (lH, br, C4-H) c~
7.4~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) Table 3 (f) Example R Z IR (cm~l) 'H - N M R Method No. (Nujol) (DMS0-d~, ~ value) 0.94 (6H, s, >C(CH3)z) 3350 1.75 (3H, d, J=611z, =C-Cl13) -CHzCH=CHCH3 1690 2.26 (3H, s, =C-CH3) 2.36 (3H, s, =C-CH3) 2dihydro- 1520 3.60 (3H, s, -C02CH3) 3.87 (2H, s, -C0zCHz-) chloride 5.00 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) 0.96 (6H, s, >C(CH3)2) 2.00 (lH, br, -C- CH) -(CH2)3C--CH 3300 2.27 (3H, s, =C-CH3) 2.37 (3H, s, =C-CH3) 21 dihydro- 1680 3.60 (3H, s, -COzCH3) 3.88 (2H, s, -C02CH2-) 2 chlorode 1520 5.00 (lH, s, C4-H) 7.4~ 8.1 (4H, m, phenyl) 9.20 (lH, br, >NH) 3200 0.98 (6H, s, >C(CH3)z) 2.25 (3H, s, =C-CH3) 22 -CHzCHzN~ 0 1690 2.35 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 2 _~
trihydro- 1520 5.00 (lH, s, C4-13) c~
chloride 7.4 ~8.2 (4H, m, phenyl) 9.20 (lH, br, >NH) Table 3 (g) Example R 2IR (cm~l) ' H--N M R Method No. (Nuiol) (DMS0-d b ~ ~ value) 0.98 (6H, s, >C(CH3) z) 2.25 (3H, s, =C-CH3) -CHzCN 3200 2.37 (3H, s, =C-CH3) 3.5$ (3H, s, -COzCH3) 23 dihydro- 1690 3.80 (2H, s, -CHzCN) 3.91 (2H, s, -C0zCHz-) 2 chloride1520 5.00 (lH, s, C4-H) 7.4~8.1 (4H, m, phenyl) 9.25 (lH, br, >NH) 3400 0.99 (611, s, >C(CH3) z) 2.27 (3H, s, =C-CH3) -CHzCHzONOz1690 2.36 (3H, s, =C-CH3) 3.60 (3H, s, -COzCH3) 24 dihydro- 1640 3.90 (2H, s, -C0zCHz-) 2 chloride1520 4.7~5.0 (3H, m, C4-H, -CH20NOz) 7.4~8.1 (411, m, phenyl) 9.20 (lH, br, >NH) 1.05 (6H, s, >C(CH3) 2) 3300 1.26 (3H, t, J=7Hz, -CHzCH3) -CHzC02CzH51690 2.40 (3H, s, =C-CH3) 3.01 (2H, s, >NCHzC-) 2 dihydro- 1520 3.60 (3H, s, -COzCH3) 3.95 (2H, s, -C0zCH
chloride 4.20 (211, s, >NCllze-) 5.00 (lH, s, C4-H) ci~
7.4~8.1 (4H, m, phenyl) 9.34 (lH, br, >NH) Table 3 (h) Example R 2IR (cm~l) lH - N M R Method No. (Nuiol) (DMSO-d~, ~ value) 1.17 (6H, s, >C(CH3) 2) 1.6~ 2.2 (4H, m, -N~H ) 3380 2.30 (3H, s, =C-CH3) 2.40 (3H, s, =C-CH3) 26 -CH2CON~ 1680 2.95 (2H, br, >NCH2C-) 3.61 (3H, s, -CO2CH3) 2 dihydro- 1650 3.99 (2H, s, -C02CH2-) 4.39 (2H, s, >NCH2Ç-) chloride1525 5.02 (lH, s, C4-H) 7.4 ~8.1 (4H, m, phenyl) 9.10 (lH, br, >NH) 1.10 (6H, s, >C(CH3)2) 2.32 (3H, s, =C-CH3) 2.41 (3H, s, =C-CH3) 2.77 (3H, d, J=5Hz, -NHCH3) -CH2CONHCH33400 2.92 (2H, s, >NCHzC-) 3.67 (3H, s, -COzCH3) -27 dihydro- 1680 4.05 (4H, s, -COzCHz-, >NCHzC,-) 2 chloride1520 5.05 (lH, s, C4-H) 7.2 ~ 8.2 (4H, m, phenyl) 8.5 ~8.8 (lH, m, -NHCH3) 0.93 (6H, s, >C(CH3)2) 2.28 (3H, s, =C-CH3) _~
-CH2CH2F 3340 2.38 (3H, s, =C-CH3) 3.60 (3H, s, -CO2CH3) c~
28 dihydro- 1680 3.88 (2H, s, -C02CHz-) 5.01 (lH, s, C4-H) 2 chloride1520 4.50, 5.30 (2H, m, >NCH2CH2CH2F) 7.46~8.20(4H, m, phenyl) 9.20 (lH, br, >NH) Z
V _ ~
V ~ o , ..
I l l V D11 I C~ ,1:~
X : :;
oo o ~ cr~ e-- x C~~0 0 C~4~ 0 Z o ~ ~:: 1 N
S~ 3~ ~ ;e '-- V N _ _ -- V
t~ I O ~ ~ I O ~ E~
A - V ~ ~ ,~ A ~
S m -- X
u~ .. ^ n O Ir --I ~ ~ X m O c~ O C~
-O
, _ . .
X ~
.. . .
_I -O
' . .
-34a-1 33776~
[Formulation Examples of Pharmaceutical Preparations]
Example 1 Formulation example for nasal drops:
Compound of this invention 0.1 mg Physiological saline solution Total 100.0 ml Example 2 Formulation example for eye drops:
Compound of this invention 0.1 mg Sterile solution for eye-drops (pH 5.0) Total 100.0 ml Example 3 Formulation example for injection (aqueous solution):
(in 2 ml ampule) Compound of this invention 1 mg Distilled water for injection Total 2 ml Example 4 Formulation example for injection (solid injection):
(in each vial) Compound of this invention 1 mg Mannitol 100 mg 1 33~764 Example 5 Formulation example for tablets -Compound of this invention 10 mg Lactose 80 mg Magnesium stearate 2 mg Talc 4 mg Add starch to make an entire amount of 120 mg.
According to the above composition, an aqueous solution of starch was added to a premixture of the compound of this invention and lactose, and this was followed by kneading. Then, the resulting mixture was dried and pulverized to thereby give a powder of uniform particles. To this powder a mixture of talc and magnesium stearate was added, and the resulting mixture was tableted to thereby produce the desired tablets.
Example 6 Formulation example for capsules Compound of this invention 5 mg Lactose 100 mg Polyvinylpyrrolidone 3 mg Add stearic acid to make an entire amount of 110 mg.
According to the above composition, polyvinylpyrrolidone and an alcohol solution of stearic acid were added to a premixture of the compound of this invention and lactose, and this was followed by kneading. Then, the resulting mixture was granulated to thereby give granules. The resulting granules, after drying, were filled in No. 4 capsular containers to thereby produce the desired capsules.
Reference example 1 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanal o CH~
H C--C--CH~--N r~l--BOC
c~3 2.9 g of N-(tert-butyloxycarbonyl)piperazine and 1.6 ml of 35% formalin were dissolved in 8 ml of acetic acid, and the resulting solution was stirred at room temperature for 30 minutes.
Then, 1.5 ml of isobutylaldehyde was added, and the resulting mixture was stirred at 50C for 12 hours. After concentration under reduced pressure, the residue was extracted with ethyl acetate and washed with saturated aqueous sodium bicarbonate.
After drying with anhydrous sodium sulfate, the solvent was evaporated under reduced pressure to give 3.9 g of a colorless powdery crystal (yield: 94%). The IR and NMR data of the compound thus obtained are as follows:
1 R (Nujo], cm~') 1720, 1700 N M R (CDC1 3, ~ value) 1.06 (611, s, >C(CH:I)Z) 1.44 (9H, s, -C(CH:~
~ H
2.3 6 ( 4 !1, t, J =S H z, -I`J~ N- l~n C
2.4~ (211, s, -Cl12N < ) 3.34 (411, t, ~=511z, -N ~ 1~l-r'oc) 9.50 (l11, s, -C1~0) 1 33~764 Reference example 2 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropanal O CH~ ~ O
~C-C -CH2- N N - C H
Formylpiperazine as the starting materlal was treated in the same manner as in Reference example 1 to give the subject compound (yield: 98%). The IR and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~') 1715, 1670 H - N M R (CDC13, ~ value) 1.09 (6H, s, >C(CI13)~) 2.50 (2l1, s, -CHzN<) H ~
2 . 4 5 ( /1 Il, m, - C O - N~N - ) 3 . 4 0 ( 'I 11, m, - C O - N N - ) 7.95 (111, br-s, >N-CHO) 9.50 (111, s, -C-CI~O) Reference example 3 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanol CH3 ~
I~OC~J2--C - CH~--1\1 N--BOC
c~3 ~ ~37764 0.14 g of sodium borohydride was dissolved in 4 ml of isopropanol, and 3.8 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanal was added. The solution was stirred at room temperature for 2 hours; then, water was added, and the solution was extracted with methylene chloride. After drying with anhydrous sodium sulfate, the solvent was evaporated under reduced pressure to give 3.8 g of a needle-like crystal (yield: 99%). The IR and NMR data of the compound thus obtained are as follows:
I R (~e~t, cn~ 3450, l70n ' H - N M R (CDCl 3, ~ value) 0.92 (611, s, >C(CI~ 3) 2) 1.45 (9l1, s, -C(CH 3) 3) 2.37 (2l~. s, -Cl3zN<) Hl 2.54 (4H, br-t, -N~-BOC) 3.45 (411, br-t, -N~_~N-BOC)-3.48 (2H, s, -CHzOH) 4.35 (lH, br, -OH) Reference example 4 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropanol ~ 11 HOCH2 - C- CH2 1\1 t\l--CH
3-(4-Formyl-1-piperazinyl)-2,2-dimethylpropanal as the starting material was treated in the same manner as in Reference example 3 to give the subject compound (yield: 85%). The IR
and NMR data of the compound thus obtained are as follows:
I ~ (Neat, cm~') 3450, 1670 N M R (CnC1~, ~ value) 0.93 (~jll, s, >(;(C11 3) z) 2.3~3 (211, s, -CII~N< ) 2. t~O (4111 m, -CO-N N-) 3.40 (411, m, -CO-N N-) 3.45 (21~, s, -CC1~z011) 4.50 (111, br, -Oli) Reference example 5 Synthesis of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl acetoacetate C~
H3 CCOCH C02 CH2 - C - CH2- N ~--BOC
3.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropanol and 30 mg of 4-dimethylaminopyridine were dissolved in 10 ml of methylene chloride, and diketene was added while cooling the solution with ice. After stirring the solution for 30 minutes under cooling with ice, water was added and the solution was extracted with methylene chloride. The resulting extract was concentrated under reduced pressure to ~0 ~ ~377~4 give 4.7 g of an oily product (yield: 97%). The IR and NMR
data of the compound thus obtained are as follows:
r~ ;3 ~, (,rll 1 ) l7~(), l72(), l7~0 N M 1~ value) O . 90 (611, s, > C (Cll 3) 2) 1 . 45 (911, ~, - C (Cll 3) 3) 2.18 (211, s, -Cl12N < ) 2 . 26 (3H, s, - COCII 3) 2.45 (411, br-t, -N N-BOC) ( H
3. 39 (411, br- t, -N~N-BOC) 3 . 45 (2H, s, - CûCH 2CO-) 3. 95 (21~, s, -COzCl{2-) Reference example 6 Synthesis of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate CH3 ~ O
H3C~OCH2CO (:~H2 - C- CH~-N r~/- CII
C~3 3-(4-Formyl-1-piperazinyl)-2,2-dimethylpropanol as the starting material was treated in the same manner as in Reference example 5 to give the subject compound (yield: 98%). The IR
and NMR data of the compound thus obtained are as follows:
I 1~ (N(~a~, cm ') 1735, 1710, IG75 '11 - N M R (CDCI~, ~ value) 0.91 (611, s, >C(CII 3) ~) 2.23 (211, s, -Cll 2 N < ) 2.27 (311, m, -COC1~3) 2.51 (411, m, -CO-N~ N-) 3.40 (41~, m, -CO-~__,N-) 3.47 (21~, s, -COCIIzCO-) 3.95 (21~, s, -COZC~12-) 7.96 (111, br-s >NCI!()) Reference example 7 Synthesis of 3~ piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate ~,NO2 \~ CH3 H3CO3C X ~ CO ~ C H 2--C - C H 2 ~N 11 9.7 g of 3-[4-(tert-butyloxycarbonyl)-1-piperazinyl]-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate was dissolved in ethanol and 50 ml of an ethanol solution of 9.9 N hydrogen chloride was added under cooling with ice. After stirring the mixed solution for 1 hour under cooling with ice, isopropyl ether was added.
The solid which separated was collected by filtration, dissolved in saturated aqueous sodium bicarbonate and extracted with ethyl acetate. The solvent was evaporated under reduced pressure and 1 33776~
acetone was added to the residue. The crystal which separated was collected by filtration to give 3.5 g of the subject compound (yield: 43%). The IR and NMR data of the compound thus obtained are as follows:
I R (Nuj ol, cm~ ') 3270, 3220, 1690, l520 ' H - N ~ R (CDC13, ~ value) 0.81 (611, s, >C(C1~3)z) 2.l5 (2~1, s, -C1121~'<) 2.32 (311, s, =C-CI13) 2.40 (311, s, =C,-CI13) 2.70 (411, br-t, --C-N1 N-) H ~ H
3.05 ~411, br - l, C-N N-) ~ H
3. ~(j (311, s, -COzCI~3) .7O (211, s, -(,() 2CII z - ) (111, s, C4-11) 7.2 ~3.3 (611, m, phenyl , > Nll) Reference example 8 Synthesis of 3~ piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate ~ CH3 H3 CO2 C ~ CO 2C H ~--C-- C H ~ ~'I ~ H
H~C CH3 CH3 460 mg of 3-(4-formyl-1-piperazinyl)-2,2-dimethylpropyl methyl 2,6-dimethyl-4-(m-nitrophenyl)-1,4-dihydropyridine-3,5_ dicarboxylate was dissolved in 1.5 ml of methanol and 0.3 ml of an ethanol solution of 9.9 N hydrogen chloride was added.
After stirring the mixed solution for one night, isopropyl ether was added. The solid which separated was collected by filtration, dissolved in saturated aqueous sodium bicarbonate and extracted with ethyl acetate. After washing with saturated solution of sodium chloride, the extract was dried with anhydrous sodium sulfate; then, the solvent was evaporated under reduced pressure to give 350 mg of the subject compound (yield: ~31~).
Reference example 9 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropanal O Cl 13 ~
HC - C - C H2 - N ~--C1-12 C~ = Ct~2 CH~
1-Allylpiperazine as the starting material was treated in the same manner as in Reference example 1 to give the subject compound (yield: 75%). The IR and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~') 1720 '~l - N M R (CDC13 ~ value) I.00 (611, s, >C(CI13).) H~l~
H H~H
2. 95 (211, d, J ~ ( l!2 -Cll=) a,. 9 -` 6. 0 (~'311 nl, vinyl) 9. 55 (1 11, i, - (,11()) a,4 Reference example 10 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropanol C~3 f__ H o CH2--C - CH2 - N ~1- C~12 C H = C H~
3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropanal as the starting material was treated in the same manner as in Reference example 3 to give the subject compound (yield: 98%). The IR and NMR data of the compound thus obtained are as follows:
! R (Neat, cm~') 3450 'H -- N M R (CDCI3, ~ value) O. 98 (6H, s, > C (CH3) z) H H
2 . 2 ~ 2 . 7 (lOH, br, - N~N-, - CH 2N < ) 1-l H ~
2. 9S (2H, d, J=6Hz, -Cl12-CII=) 3. 50 (211, s, -CH20H) 3. 70 (111, br, -OH) Reference example 11 Synthesis of 3-(4-allyl-1-piperazinyl)-2,2-dimethylpropyl acetoacetate c~3 /--\
1~3 CCOCH2 C02 C1~2 - C - C H2 - N N--CH~ CH = CH2 \ /
CH~
1 337~64 3-(4-Allyl-1-piperazinyl)-2,2-dimethylpropanol as the starting material was treated in the same manner as in Reference example 5 to give the subject compound (yield: 97%). The IR
and NMR data of the compound thus obtained are as follows:
I R (Neat, cm~ ') 1740, 1720 ' H -- N M R (CDCI3, ~ value) 0.90 (6H, s, >C(CH3)z) 2. 28 (3H, s, -COCH3) H ~H
2. 4 ~2.7 (lOH, br, -N~N-, -CIIzN < ) 3. 00 (2H, d, J=611z, -CH2-CII=) 3. 45 (2H, s, -COCH2CO-) 4. lS (2H, s, -CO2CHz-) 5.0 ~6.0 (3H, m, vinyl)
Claims (21)
1. A 1,4-dihydropyridine derivative represented by the general formula:
(I) [in which:
X1 and X2 independently represent hydrogen, difluoro-methyl, trifluoromethyl, monofluoromethoxy, difluoromethoxy, chlorine, bromine, fluorine, cyano or nitro;
R1 represents a lower alkyl;
R2 represents:
(i) a saturated or unsaturated C1-C5 aliphatic acyl, (ii) an acyl represented by the formula:
R2'-(B)n-CO-(in which R2' is an aromatic group selected from the group consisting of phenyl and naphthyl or a 5- or 6-membered hetero-cyclic group having a heteroatom selected from the group consist-ing of nitrogen and oxygen, B is a saturated or unsaturated C1-C3 aliphatic group, and n is 0 or 1), wherein each of the said aliphatic acyl and the aromatic or heterocyclic groups may be substituted by halogen, hydroxyl, carboxyl, lower alkoxy, lower alkanoyl or lower alkanoylamino, (iii) (C1-C5)alkoxy-carbonyl, (iv) an acyl-(C1-C5)alkyl in which the acyl is as defined in (i), (ii) or (iii) above, (v) an N-(C1-C5)alkyl-substituted carbamoyl-(C1-C5)-alkyl, (vi) a (C1-C5)alkoxy-(C1-C5)alkyl, (vii) a (C1-C5)alkoxy-carbonyl-(C1-C5)alkyl, (viii) an acyloxy-(C1-C5)alkyl in which the acyl is as defined in (i), (ii) or (iii) above, (ix) a nitrato(C1-C5)alkyl, (x) a cyano(C1-C5)alkyl, (xi) a heterocycle-(C1-C5)alkyl in which the hetero-cycle is a 5- or 6-membered heterocycle having a heteroatom selected from the group consisting of nitrogen and oxygen, (xii) a halo-(C1-C5)alkyl in which halo is fluorine, chlorine or bromine, (xiii) a C2-C5 alkenyl, or (xiv) a C2-C5 alkynyl, A represents an alkylene comprising a carbon atom to which two methyl groups are bonded and having in total 5 to 10 carbon atoms, and, m represents an integer of 1, 2 or 3], or an acid addition salt thereof.
(I) [in which:
X1 and X2 independently represent hydrogen, difluoro-methyl, trifluoromethyl, monofluoromethoxy, difluoromethoxy, chlorine, bromine, fluorine, cyano or nitro;
R1 represents a lower alkyl;
R2 represents:
(i) a saturated or unsaturated C1-C5 aliphatic acyl, (ii) an acyl represented by the formula:
R2'-(B)n-CO-(in which R2' is an aromatic group selected from the group consisting of phenyl and naphthyl or a 5- or 6-membered hetero-cyclic group having a heteroatom selected from the group consist-ing of nitrogen and oxygen, B is a saturated or unsaturated C1-C3 aliphatic group, and n is 0 or 1), wherein each of the said aliphatic acyl and the aromatic or heterocyclic groups may be substituted by halogen, hydroxyl, carboxyl, lower alkoxy, lower alkanoyl or lower alkanoylamino, (iii) (C1-C5)alkoxy-carbonyl, (iv) an acyl-(C1-C5)alkyl in which the acyl is as defined in (i), (ii) or (iii) above, (v) an N-(C1-C5)alkyl-substituted carbamoyl-(C1-C5)-alkyl, (vi) a (C1-C5)alkoxy-(C1-C5)alkyl, (vii) a (C1-C5)alkoxy-carbonyl-(C1-C5)alkyl, (viii) an acyloxy-(C1-C5)alkyl in which the acyl is as defined in (i), (ii) or (iii) above, (ix) a nitrato(C1-C5)alkyl, (x) a cyano(C1-C5)alkyl, (xi) a heterocycle-(C1-C5)alkyl in which the hetero-cycle is a 5- or 6-membered heterocycle having a heteroatom selected from the group consisting of nitrogen and oxygen, (xii) a halo-(C1-C5)alkyl in which halo is fluorine, chlorine or bromine, (xiii) a C2-C5 alkenyl, or (xiv) a C2-C5 alkynyl, A represents an alkylene comprising a carbon atom to which two methyl groups are bonded and having in total 5 to 10 carbon atoms, and, m represents an integer of 1, 2 or 3], or an acid addition salt thereof.
2. The compound as claimed in claim 1 in which R2 is the acylalkyl (iv), the N-alkyl-substituted carbamoylalkyl (v), the 47a alkoxyalkyl (vi), the cyanoalkyl (x), the heterocycle-alkyl (xi), the alkenyl (xiii) or the alkynyl (xiv), all defined in claim 1.
3. The compound as claimed in claim 1 in which R2 is the alkenyl (xiii) or the alkynyl (xiv), both defined in claim 1.
47b
47b
4. The compound as claimed in claim 1, 2 or 3 in which A
is an alkylene having in total not less than 5 and not more than 8 carbons.
is an alkylene having in total not less than 5 and not more than 8 carbons.
5. 2,2-Dimethyl-3-[4-(2-methyl-2-propenyl)-1-piperazinyl]-propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyr-idinedicarboxylate or its dihydrochloride.
6. 2,2-Dimethyl-3-[4-(2-butenyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its dihydrochloride.
7. 2,2-Dimethyl-3-(4-cinnamyl-1-piperazinyl)propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxy-late or its dihydrochloride.
8. 2,2-Dimethyl-3-[4-(2-butynyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its dihydrochloride.
9. 2,2-Dimethyl-3-[4-(4-pentynyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its dihydrochloride.
10. 2,2-Dimethyl-3-(4-piperidinoethyl-1-piperazinyl)propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its trihydrochloride.
11. 2,2-Dimethyl-3-[4-(2-hydroxyethyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedi-carboxylate or its dihydrochloride.
12. 2,2-Dimethyl-3-(4-benzoyl-1-piperazinyl)propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxy-late or its hydrochloride.
13. 2,2-Dimethyl-3-[4-(1-methyl-2-oxopropyl)-1-piperazinyl]-propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridinedicarboxylate or its dihydrochloride.
14. 2,2-Dimethyl-3-[4-(2-cyanoethyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its dihydrochloride.
15. 2,2-Dimethyl-3-[4-(2-furoyl)-1-piperazinyl]propyl methyl 1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-3,5-pyridine-dicarboxylate or its hydrochloride.
16. A pharmaceutical composition comprising the 1,4-dihy-dropyridine derivative of the formula as defined in claim 1 or a pharmaceutically acceptable acid addition salt thereof and one or more pharmaceutically acceptable additives, wherein the 1,4-dihydropyridine derivative or its salt is present in an amount sufficient for therapeutic or preventive use for circulatory di-seases.
17. The pharmaceutical composition as claimed in claim 16, in which the 1,4-dihydropyridine derivative or its salt is present in an amount sufficient as a calcium antagonist.
18. The calcium antagonist as claimed in claim 17 which is an antihypertensive agent, antianginal agent, cerabral circu-lation improving agent, peripheral circulation improving agent, renal function improving agent, antiarteriosclerosis drug, smooth muscle relaxant, antiallergic, therapeutic drug for cataract or therapeutic drug for glaucoma.
19. The pharmaceutical composition as claimed in claim 16 which is in any form of pharmaceutical preparation selected from eye drops, nasal drops, inhalations, injections and liniments.
20. A method of producing 1,4-dihydropyridine derivatives of the general formula (I) as defined in claim 1, which comprises:
(1) reacting together a compound of the general formula:
, a compound of the general formula:
and a compound of the general formula:
, (2) reacting a compound of the general formula:
with a compound of the general formula:
, (3) reacting a compound of the general formula:
or a reactive derivative thereof with a compound of the general formula:
, or (4) reacting a compound of the general formula:
with a compound of the general formula:
R2-Y4 , wherein X1, X2, R1, R2, A and m are as defined in claim 1, Y2 represents a reactive group selected from the group consisting of halogen, p-toluenesulfonate and methanesulfonate and Y4 represents halogen or p-toluenesulfonate.
(1) reacting together a compound of the general formula:
, a compound of the general formula:
and a compound of the general formula:
, (2) reacting a compound of the general formula:
with a compound of the general formula:
, (3) reacting a compound of the general formula:
or a reactive derivative thereof with a compound of the general formula:
, or (4) reacting a compound of the general formula:
with a compound of the general formula:
R2-Y4 , wherein X1, X2, R1, R2, A and m are as defined in claim 1, Y2 represents a reactive group selected from the group consisting of halogen, p-toluenesulfonate and methanesulfonate and Y4 represents halogen or p-toluenesulfonate.
21. A pharmaceutical composition comprising the 1,4-di-hydropyridine derivative of the formula as defined in claim 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 or a pharmaceutically acceptable acid addition salt thereof and one or more pharma-ceutically acceptable additives, wherein the 1,4-dihydropyridine derivative or its salt is present in an amount sufficient for therapeutic or preventive use for circulatory diseases.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62057717A JPH0688973B2 (en) | 1987-03-12 | 1987-03-12 | 1,4-dihydropyridine derivative |
JP057717/1987 | 1987-03-12 |
Publications (1)
Publication Number | Publication Date |
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CA1337764C true CA1337764C (en) | 1995-12-19 |
Family
ID=13063696
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Application Number | Title | Priority Date | Filing Date |
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CA000561305A Expired - Lifetime CA1337764C (en) | 1987-03-12 | 1988-03-11 | 1,4-dihydropyridine derivatives and pharmaceutical composition thereof |
Country Status (10)
Country | Link |
---|---|
US (1) | US4937242A (en) |
EP (1) | EP0289746B1 (en) |
JP (1) | JPH0688973B2 (en) |
KR (1) | KR960009427B1 (en) |
AT (1) | ATE113035T1 (en) |
AU (1) | AU610130B2 (en) |
CA (1) | CA1337764C (en) |
DE (1) | DE3851856T2 (en) |
DK (1) | DK174867B1 (en) |
ES (1) | ES2061534T3 (en) |
Families Citing this family (12)
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US5137889A (en) * | 1983-12-02 | 1992-08-11 | Otsuka Pharmaceutical Co., Ltd. | Dihydropyridine derivatives and process for preparing the same |
US5166148A (en) * | 1990-07-09 | 1992-11-24 | The Du Pont Merck Pharmaceutical Company | 2-amino-1,4-dihydropyridine derivatives with calcium agonist and alpha1 -antagonist activity |
US5767131A (en) * | 1993-04-05 | 1998-06-16 | Synaptic Pharmaceutical Corporation | Dihydropyridines and new uses thereof |
IL109220A0 (en) * | 1993-04-05 | 1994-07-31 | Synaptic Pharma Corp | Dihydropyridines and new uses thereof |
US6211198B1 (en) | 1993-04-05 | 2001-04-03 | Synaptic Pharmaceutical Corporation | Dihydropyridines and new uses thereof |
AU8142794A (en) * | 1993-11-17 | 1995-06-06 | Byk Nederland Bv | Use of substituted 1,4-dihydropyridine to reduce intraocular pressure |
ES2317650T3 (en) * | 1996-10-28 | 2009-04-16 | Senju Pharmaceutical Co., Ltd. | PHARMACES TO IMPROVE OCULAR CIRCULATORY DISORDERS. |
ATE290910T1 (en) * | 1998-07-16 | 2005-04-15 | Univ Pennsylvania | USE OF A3-ADENOSINE ANTAGONISTS FOR THE PRODUCTION OF A MEDICINAL PRODUCT FOR LOWERING THE INTERNAL EYE PRESSURE |
CA2360689A1 (en) | 1999-01-29 | 2000-08-03 | Senju Pharmaceutical Co., Ltd. | Anti-inflammatory agents and inhibitors against increase in ocular tension caused by irradiation with lasers, containing 1,4-dihydropyridine derivatives |
DE60014629T2 (en) * | 1999-04-14 | 2006-02-23 | Senju Pharmaceutical Co., Ltd. | 1,4-dihydropyridine derivatives as inhibitors of visual hypofunction induced by optic nerve cell injury induced by factors other than optical circulatory disorders. |
US6566359B1 (en) | 2002-05-20 | 2003-05-20 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | 2,4,6-trimethyl-1,4-dihydro-pyridine-3,5-dicarboxylic acid esters as neuroprotective drugs |
KR101419213B1 (en) * | 2013-05-22 | 2014-07-14 | 인하대학교 산학협력단 | Composition for skin whitening comprising methyl 5-cyano-6-{[2-(4-methoxyphenyl)-2-oxoethyl]sulfanyl}-2-methyl-4-(2-thienyl)-1,4-dihydro-3-pyridinecarboxylate as effective component and uses thereof |
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DE2005116C3 (en) * | 1970-02-05 | 1980-02-14 | Bayer Ag, 5090 Leverkusen | Symmetrical 1,4-dihydropyridine-3,5-dicarboxylic acid esters |
US3950336A (en) * | 1972-03-06 | 1976-04-13 | Bayer Aktiengesellschaft | 2,6-diamino-1,4-dihydropyridine derivatives |
US3951993A (en) * | 1972-03-06 | 1976-04-20 | Bayer Aktiengesellschaft | 2-Amino-4,5-dihydropyridine derivatives and process for their preparation |
US4031104A (en) * | 1972-04-18 | 1977-06-21 | Bayer Aktiengesellschaft | 1,4-Dihydropyridine carboxylic acid esters |
US3959296A (en) * | 1972-06-10 | 1976-05-25 | Bayer Aktiengesellschaft | 1,4-Dihydropyridines |
US3951991A (en) * | 1972-08-31 | 1976-04-20 | Bayer Aktiengesellschaft | 4-Aryl-6-amino-3,4-dihydropyrid-2-one-3,5-dicarboxylic acid ester |
DE2248150A1 (en) * | 1972-09-30 | 1974-04-04 | Bayer Ag | DIHYDROPYRIDINE POLYESTER, METHOD FOR MANUFACTURING AND USING THEM AS A MEDICINAL PRODUCT |
DE2508181A1 (en) * | 1975-02-26 | 1976-09-09 | Bayer Ag | 1,4-DIHYDROPYRIDINCARBONIC ACID ARAL KYLESTER, METHOD FOR MANUFACTURING AND USING THEY AS A MEDICINAL PRODUCT |
CS228917B2 (en) * | 1981-03-14 | 1984-05-14 | Pfizer | Method of preparing substituted derivatives of 1,4-dihydropyridine |
DE3124673A1 (en) * | 1981-06-24 | 1983-01-13 | Bayer Ag, 5090 Leverkusen | SUBSITUATED 2-AMINO-PYRIDINE DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF, THEIR USE IN MEDICINAL PRODUCTS AND THE PRODUCTION THEREOF |
NZ201395A (en) * | 1981-07-30 | 1987-02-20 | Bayer Ag | Pharmaceutical compositions containing 1,4-dihydropyridines and certain of these dihydropyridines |
HU192166B (en) * | 1982-08-06 | 1987-05-28 | Banyu Pharma Co Ltd | Process for producing 2-carbamoyl-oxy-alkyl-1,4-dihydro-pyridine derivatives |
US4656181A (en) * | 1982-11-24 | 1987-04-07 | Cermol S.A. | Esters of 1,4-dihydropyridines, processes for the preparation of the new esters, and medicaments containing the same |
AU561213B2 (en) * | 1983-06-02 | 1987-04-30 | Teijin Limited | 1, 4-dihydropyridine derivative |
US4603135A (en) * | 1983-10-17 | 1986-07-29 | Takeda Chemical Industries, Ltd. | Substituted piperazinyl alkyl esters of 2-amino-4-aryl-1,4-dihydro-6-alkyl-3,5-pyridinedicarboxylates |
GB8401288D0 (en) * | 1984-01-18 | 1984-02-22 | Pfizer Ltd | Therapeutic agents |
JPS6157556A (en) * | 1984-08-29 | 1986-03-24 | Teijin Ltd | 1,4-dihydropyridine derivative, its preparation, and drug comprising it as active ingredient |
JPS61137861A (en) * | 1984-10-26 | 1986-06-25 | バイエル・アクチエンゲゼルシヤフト | 1,4-dihydropyridine-hydroxyamines |
DE3514865A1 (en) * | 1985-04-25 | 1986-11-06 | Bayer Ag, 5090 Leverkusen | 1,4-DIHYDROPYRIDINE DERIVATIVES, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE IN MEDICINAL PRODUCTS |
-
1987
- 1987-03-12 JP JP62057717A patent/JPH0688973B2/en not_active Expired - Lifetime
-
1988
- 1988-03-01 AU AU12519/88A patent/AU610130B2/en not_active Expired
- 1988-03-08 US US07/165,705 patent/US4937242A/en not_active Expired - Lifetime
- 1988-03-10 AT AT88103866T patent/ATE113035T1/en not_active IP Right Cessation
- 1988-03-10 DE DE3851856T patent/DE3851856T2/en not_active Expired - Lifetime
- 1988-03-10 DK DK198801296A patent/DK174867B1/en not_active IP Right Cessation
- 1988-03-10 EP EP88103866A patent/EP0289746B1/en not_active Expired - Lifetime
- 1988-03-10 ES ES88103866T patent/ES2061534T3/en not_active Expired - Lifetime
- 1988-03-11 CA CA000561305A patent/CA1337764C/en not_active Expired - Lifetime
- 1988-03-12 KR KR1019880002667A patent/KR960009427B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0289746A3 (en) | 1989-09-20 |
EP0289746A2 (en) | 1988-11-09 |
DK129688A (en) | 1988-09-13 |
KR890002102A (en) | 1989-04-08 |
DK129688D0 (en) | 1988-03-10 |
EP0289746B1 (en) | 1994-10-19 |
ATE113035T1 (en) | 1994-11-15 |
JPS63225355A (en) | 1988-09-20 |
US4937242A (en) | 1990-06-26 |
DK174867B1 (en) | 2004-01-05 |
KR960009427B1 (en) | 1996-07-19 |
AU610130B2 (en) | 1991-05-16 |
AU1251988A (en) | 1988-09-15 |
DE3851856T2 (en) | 1995-03-16 |
ES2061534T3 (en) | 1994-12-16 |
DE3851856D1 (en) | 1994-11-24 |
JPH0688973B2 (en) | 1994-11-09 |
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