CA1320163C - Chimeric glycoproteins containing immunogenic segments of the glycoproteins of human respiratory syncytial virus - Google Patents

Abstract

ABSTRACT
CHIMERIC GLYCOPROTEINS CONTAINING IMMUNOGENIC SEGMENTS OF THE
GLYCOPROTEINS OF HUMAN RESPIRATORY SYNCYTIAL VIRUS
This invention encompasses DNA compositions encoding novel chimeric glycoproteins which are useful for preparing virus specific immune responses against human respiratory syncytial virus. The DNA
compositions include structural genes coding for the glycoproteins and expression and replication plasmids containing the structural genes, Host cells transformed with the above DNA compositions, vaccines made from the glycoproteins and methods for protecting humans by inoculation with said vaccines are also part of this invention.

Description

20~3 -1- 4340.P C~l CHIMERIC GLYCOPROTEINS CONTAINING IMMUNOGENIC SEGMENTS
OF THE GLYCOPROTEI~S OF HUMAN RESPIRATORY SYNCYTIAL VIRUS
Field of the Invention This invention encompasses DNA compositions encoding novel chimeric glycoproteins which are useful for preparing virus specific immune responses against human respiratory syncytial virus, HP~SV.
The DNA compositions include structural genes coding for the glyco-proteins and expression and replication plasmids containing the structural genes. Host cells transformed with the above DNA composi-tions, vaccines made from the glycoproteins and methods for protect-ing humans by inoculation with said vaccines are also part of this invention.
Background HRSV was discovered in 1956 and is found worldwide. It causes upper and lower respiratory tract disease particularly in infants and young children. About 30 percent of hospitalized young children with acute respiratory disease have respiratory syncytial virus infection.
In older children and adults the disease is milder. In infants this severe illness often requires hospitalization.
Infections with respiratory syncytial virus are referable to all segments of the respiratory tract, are usually associated with fever, cough, runny nose, and fatigue, and are diagnosed clinically as bronchitis, bronchiolitis, pneumonia, croup, or viral infection. In older children and adults the virus is generally limited to replica-tion in the upper respiratory tract. Infants may be ~ore severely involved when the virus extends into the lungs. Lung damage can be permanent, Primary infection with respiratory syncytial virus occurs early i.n life, usually before ~ years of age. Among children, illness caused by this virus tends to occur at least once each year in rather - sharply defined outbreaks of several months duration. Epidemics are sharply circumscribed, generally for 3 to 5 months. In family studies, children in early school years frequently introduce the virus into the home, infecting younger members of the family more severely than other family members. The clinical consequence of infection is most severe on first experience and becomes milder in older individuals who are immunologically experienced.
Secondary effects of respiratory syncytial virus can range from .

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~32~163

-2-inapparent infection to severe pneumonia and death, Inflammation of the respiratory tract is responsible for ~ost symptoms, Complete recovery in most cases occurs in one to three weeks with the produc-tion of antibody which appears to persist throughout life, In the United States about 30 percent of l-year-old infants and 95 percent of 5-year-old children have circulating respiratory syncytial virus antibody, Reinfections in older infants, children, and adults with antibody are mostly mild upper respiratory illnesses in the form of colds, Although low yields of virus in cell culture have hindered HRS~J
research, the virus has been well studied, HRSV is a paramyxovirus containing a single negative strand of RNA which is transcribed into 10 predominantly monocistronic messengers, ~he messengers have been isolated and translated in vitro. The products have been charac-terized by gel electrophoresis, peptide mapping and immuno-precipita-tion as being similar to structural proteins isolated from virions, The structural proteins include a major nucleocapsid protein (N; MW
ca, 42,000), a nucleocapsid phosphoprotein (P; MW ca. 34,000), a large nucleocapsid protein (L; MW ca, 200,000), an envelope matrix protein (M; MW ca, 26,000), a matrix glycoprotein (ca. 22,000) and two envelope glycoproteins, the fusion glycoprotein (F; MW ca. 68,000 to 70,000) and a second, methionine poor glycoprotein (G; MW ca, 84,000 to 90,000), In addition, a virally encoded protein of about 9,500 daltons and other small proteins are known to be present in infected cells, Collins, et al,, Identification of a tenth mRNA of HRSV and assignment of polypeptides to the 10 viral genes, J, of Virol, 49:572 -578 (1984) and references cited therein. Additional work describing the molecular biology of HSRV includes: (1) Collins, et al,, Nucleotide Sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus, Proc. Natl, Acad, Sci,, USA, 81:7683-7687 (December 1984) disclosing the gene sequence for the F glycoprotein; (2) Collins, et al,, The ~A Protein Gene of Human Respiratory Syncytial Virus: Nucleotide Sequence of the mRNA
and a Related Polycistronic Transcript, Virology, 141:283-291 (1985) disclosing the gene sequence for the lA protein; (3) Collins, et al,, The Envelope-Associated 22K Protein of Human Respiratory Syncytial Virus: Nucleotide Sequence of the mRNA and a Related Polytranscript, J. of Virol., 54(No.1):65-71 (Apr, 1985) disclosing the gene sequence 1 3 ~ 3

-3-for the 22K protein; (4) Wertz, et al., Nucleotide sequence of the G
protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein, Proc. Natl. Acad. Sci., IJSA, 82:4075-4079 (June 1985) disclosing the gene sequence for the G glycoprotein;
and (5) Collins, et al., Correct Sequence for the Major Nucleocapsid Protein m~NA of Respiratory Syncytial Virus, Virology, 146:69-77 (1985) disclosing the gene sequence for the ~ protein.
The F and G glycoproteins of HRSV have similar counterparts in the other paramyxoviruses. Like HRSV, other paramyxoviruses have an F glycoprotein which is associated with fusion of cell membranes, P.W. Choppin and A. Scheid, Rev. Infect. Dis. 2:40-61, (1980); Merz, et al., J. Exp. Med. 151:275-288, (1980). The active paramy~.ovirus F
protein consists of two disulfide-linked subunits, Fl and F2, which are generated from an inactive precursor (Fo) by a specific internal cleavage by cellular proteases, Scheid and Choppin, Virol. 80:54-66 (1977). The second major glycoprotein for most paramyxoviruses is termed the HN protein, and is associated with the hemagglutinin and neuraminidase activities of these viruses. Although the HRSV G pro-tein does not have the above en~ymatic activities, both the G and HN
glycoproteins are associated with attachment of virus. Also, these glycoproteins are structurally similar in that they have an unusual hydrophobic signal~anchor region at their amino-terminus, Wertz, et al., PNAS 82:4075-4079 (1985); Elango, et al., J. Virol. 57:481-489 (1986).
There are no available effective vaccines to combat HRSV.
Multiple attempts have been made to obtain an effective vaccine against HRSV. Friedewald, et al., Journal of the American Medical Association, 204:690-694 (20 May 1968), describe the propagation of respiratory syncytial virus in bovine embryonic kidney tissue cul-ture. Virus grown at 34C or 28C did not decrease in infectivity or virulence. HRSV grown at 26C, while associated with a decrease in infectivity for adults, could not be considered for use in prevention of infection in adults since the virus had limited infectivity and was poorly immunogenic.
Kim, et al., Pediatrics, 48:745-755 (November 1971) disclose that inactivated respiratory syncytial virus vaccine prepared from virus grown at 26C stimulated the development of high levels of serum antibody in infants and children from 6 months to 13 years in .~:

~4~ 132~163 age but did not prevent infection.
McIntosh, et al., Pediatric Research, 8:689-696 (1974) discuss two experimental live respiratory syncytial virus vaccines, one prepared from virus grown at 26C. and the other, prepared from a temperature sensitive mutant which grew well at 32C and not at all at 37C. or higher. The first vaccine was unsatisfactory as it did not protect against infection when the interval between vaccination and challenge was greater than 4 months. The second vaccine was also unsatisfactory in that it apparently lost its temperature sensitivity in some vaccinees.
Craighead, Journal of Infectious Diseases, 131:749-753 (June 1975~ discusses tests conducted in 1966 wherein several groups of investigators tested in infants and young children a formaldehyde-treated, alum-precipitated virus grown in tissue culture. Upon subsequent exposure to wild virus the vaccine recipients exhibited an accentuated pattern of respirato~y tract disease. Craighead con-cludes that immunization with formaldehyde treated virus enhanced the severity of the disease.
Uright, et al., Journal of Pediatrics, 88:931-936 (June 1976) describe the evaluation in infants of a temperature sensitive live attenuated respiratory syncytial vaccine, While this vaccine when administered at a dosage level sufficiently high to infect all seronegative infants caused mild upper respiratory illness, lowering the dose did not achieve an acceptable level of infectivity. The virus was also genetically lmstable as there was evidence of loss of temperature sensitivity in one vaccinee. There was no evidence for potentiation of natural illness with this vaccine and reinfection occurred among vaccinees.
U.S. patent Nos. 4,122,167 and 4,145,252 describe a method for attenuating virions by serial passage ~through human diploid lung fibroblasts and U.S. patent No. 4,517,304 discloses a method for producing immunogenically active HRSV proteins upon the cell mem-branes of susceptible cells grown in culture. These cells are then injected into a host to elicit an immune response.
Information Disclosure Statement The recombinant vaccinia virus expression system is known to separately express the G and F~ glycoproteins of HRSV, Ball, et al, Expression of the Major Glycoprotein G of Human Respiratory Syncytial :: :

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Virus f~om Recombinant Vaccinia Virus Vectors, P.N A S., USA, 83:246-250 (1986) and Olmsted, et al ,Expression of the F Glycoprotein of Respiratory Syncytial Virus by a Recombinant Vaccinia Virus:
Comparison of the Individual Contributions of the F and G Glycopro-teins to Host Immunity, P N A.S , USA, 83:7~62-7466 (1986). These two glycoproteins were also demonstrated to induce immunoprotection in mammals against a live HRSV virus challenge, Stott, et al., Human Respiratory Syncytial Virus Glycoprotein G Expressed from Recombinant Vaccinia Virus Vector Protects Mice Against Live-virus Challenge, Journal of Virology 67: 607-613 ~1986); Walsh, et al., Immunization with Glycoprotein Subunits of Respiratory Syncytial Virus to Protect Cotton Rats Against Viral Infection, Journal of Infectious Diseases, 1198-1204 (1987); Wertz, et al , Expression of the Fusion Protein of Human Respiratory Syncytial Virus from Recombinant Vaccinia Virus Vectors and Protection of Vaccinated Mice, Journal of Virology, 293-301 (1987); Elango, et al., Resistance to Human Respiratory Syncytial Virus (RSV) Infection Induced by Immunization of Cotton Rats with a Recombinant Vaccinia Virus Expressing the RSV G Glycoprotein, Proc.
Natl. Acad. Sci. USA, 1906-1910 (1986).
Summary of the Invention This invention encompasses a polypeptide comprising a signal sequence and at least one immunogenic fragment from both human respiratory syncytial virus glycoproteins F and G. The use of this protein as a vaccine, methods to prevent HRSV-related disease and preparation of this protein using recombinant techniques are also part of this invention.
Detailed Description The following defined terms are used in this specification. The phrase "cell culture" refers to the containment of growing cells derived from either a multicellular plant or animal which allows for the cells to remain viable outside the original pIant or animal. The term "downstream" identifies sequences proceeding farther in the direction of expression; for example, the coding region is downstream from the initiation codon. The term "microorganism" includes both single cellular prokaryote and eukaryote organisms such as bacteria, actinomycetes and yeast. The term "operon" is a complete unit of gene expression and regulation, including structural genes, regulator genes and control elements in DNA recognized by regulator gene prod-uct. The term "plasmid" refers to an autonomous self-replicating extrachromosomal circular DNA and includes both the expression and nonexpression types. Where a recombinant microorganism or cell culture is described as hosting an expression plasmid the phrase "expression plasmid" includes both extrachromosomal circular DNA and DNA that has been incorporated into the host chromosome(s). Where a plasmid is being maintained by a host cell, the plasmid is either being stably replicated by the cells during mitosis as an autonomous structure or as an incorporated portion of the host's genome. The term "promoter" is a region of DNA involved in binding the RNA poly-merase to initiate transcription. The phrase "DNA sequence" refers to a single or double stranded DNA molecule comprised of nucleotide bases, adenosine, thymidine, cytosine and guanosine. The phrase 1lessentially pure" refers to a composition of protein that contains no paramyxovirus protein other than the desired recombinant chimeric glycoprotein. Although the essentially pure proteins may be contam-inated with low levels of host cell constituents, the protein is devoid of contaminating structural and non-structural viral protein produced by replicating paramyxoviruses. The phrase "suitable host"
refers to a cell culture or microorganism that is compatible with a recombinant plasmid and wil]. permit the. plasmid to replicate, to be incorporated into its genome or to be expressed. The term "upstream"
identifies sequences proceeding in the opposite direction from expression; for example, the bacterial promoter is upstream from the transcription unit, the initiation codon is upstream from the coding region.
This invention involves a series of molecular genetic manipula-tions that can be achieved in a variety of known ways. The manipula-tions can be summarized as obtaining a cDNA of the protein, the cloning and replication of the cDNA in E. coli and the expression of the desired cDNA in a suitable host. The following descriptions will detail the various methods available to express the protein and are followed by specific examples of preferred methods. The specific sequence and base numbering positions for a particular polypeptide, glycoprotein ~G, is given in Chart 9.
Generally, the nomenclature and general laboratory procedures required in this invention can be found in Maniatis, et al., Molecu-lar Cloning A Laboratory Manual, Cold Spring Harbor Laboratory, Cold ` -7- ~320~63 Spring Harbor, ~ew York, 1982 (Maniatis).
All E. coli strains are grown on Luria broth (LB~ with glucos~, Difco's Antibiotic Medium #2 and M9 medium supplemented with glucose and acid-hydrolyzed casein amino acids. Strains with resistance to antibiotics were maintained at the drug concentrations described in Maniatis. Transformations were performed according to the method described by Rowekamp and Firtel, Dev. Biol., 79:409-418 (1980).
All enzymes were used accordine to the manufacturer's instruc-tions. Transformants were analyzed by colony hybridization as described in Grunstein and Wallis, Methods in Enzymology, 68:379-388.
After hybridization, the probes are removed and saved, and the filters are washed in 0.1~ SDS, 0.2x SSC for a total of 3 hours with 5 changes of 400 ml each. Filters are thoroughly air dried, mounted, and autoradiographed using Kodak~ X-OMAT AR film and Dupont Cronex*
Lightnening Plus intensifying screens for 1~ hours at -70 C.
For sequencing of plasmids, purified plasmid DNA is prepared according to the methods described in Maniatis. End-labeled DNA
fragments are prepared and analyzed by the chemical sequencing meth-ods of Maxam and Gilbert with modifications described by Collins and~ertz, J. Viral. 54:65-71 (1985).
Nucleotide sizes are given in either kilobases (kb) or basepairs (bp~. These are estimates derived from agarose gel electrophoresis.
The first step in obtaining expression of protein is to obtain the DNA sequence coding for the protein from cDNA clones. This sequence is then cloned into an expression plasmid which is capable of directing transcription of the gene and allowing efficient trans-lation of the transcript. The library method for obtaining cDNA
encoding protein has been described generally in Maniatis, and specifically in Collins and Wertz, cDNA Cloning and Transcriptional Mapping of Nine Polyadenylated RNAs Encoded by the Genome of HRSV, Proc. Natl. Acad. USA 80: 3208-3212 (1983) and the related documents Elango, N., et al., Resistance to Human Respiratory Syncytial Virus (RS~) Infection Induced by Immunization of Cotton Rats with a Recombinant Vaccinia Virus Expressing the RSV G Glycoprotein, Proc.
Natl. Acad. Sci. USA, 1906-1910 (198~) and Olmstead R.A. et al., Expression of the F Glycoprotein of Respiratory Syncytial Virus by a Recombinant Vaccinia Virus: Comparison of the Individual Contribu-* tras~e mark -8- 13201~
tions of the F and G glycoproteins to Host Immunity, Proc. ~Jatl.
Acad. Sci. USA, 7462-7466 (1986).
Clones are prepared by inserting the cDNA into PstI cleaved pBR322 to which homopolymer tracts of dGTP have been enæymatically added to the 3'ends at the cleavage site. Homopolymer tracts of dCT~
are enzymatically added to the 3' termini of the cDNA molecules according to the methods described by Maniatis. Ideally, 10-30 resi-dues of dCTP or dGTP should be added to maximize cloning efficiency.
The cDNA and plasmid are annealed together and transformed into E.
coli. The clones containing full length cDNA are detected by probes of labeled viral cDNA or oligonucleotides complementary to portions of the gene sequences, Eollowed by restriction enzyme analysis and DNA sequencing.
Oligonucleotides are chemically synthesi~ed according to the solid phase phosphoramidite triester method first described by Beaucage and Caruthers, Tetrahedron Letters, 22(20):1859-1862 (1981) using an automated synthesizer, as described in Needham-VanDevanter, et al., Nucleic Acids Res., 12:6159-6168 (1984). Purification of oligonucleotides is by either native acrylamide gel electrophoresis or by anion-exchange HPLC as described in Pearson and Regnier, J.
Chrom., 255:137-149 (1983).
The sequence of the synthetic oligonucleotides can be verified using the chemical degradation method of Naxam and Gilbert, Grossman and Moldave, eds., Academic Press, New York, Methods in Enzymology, 25 65:499-560 (1980).
To obtain high level expression of a cloned gene in a prokaryo-tic system, it is essential to construct expression vectors which contain, at the minimum, a strong promoter to direct mRNA transcrip-tion, a ribosome binding site for translational initiation, and a transcription terminator. Examples of regulatory regions suitable for this purpose are the promoter and operator region of the E. coli tryptophan biosynthetic pathway as described by Yanofsky, Kelley, and Horn, J. Bacteriol., 158:1018-1024 (1984) and the leftward promoter of phage lambda (PL) as described by Herskowitz and Hagen, Ann. Rev.
3~ Genet.j I4:399-445 (1980).
The proteins produced in E. coli will not fold properly due to the presence of cysteine residues and to the lack of suitable post-translational modifications. During purification from E. coli, the -9- ~ 2 ~
expressed proteins must first be denatured and then renatured. This can be accomplished by solubilizing the E. coli produced proteins in guanidine HCl and reducing all the cysteine residues with ~-mercapto-ethanol. The protein is then renatured either by slow dialysis or by gel filtration, U.S. Patent No. 4,511,503.
Detection of proteins is achieved by methods known in the art such as radioimmunoassays, or ~estern blotting techniques or immuno-precipitation. Purification from E. coli can be achieved following procedures described in U.S. Patent No. 4,511,503.
Expression of heterologous proteins in yeast is well known and described. Methods in Yeast Genetics, Sherman, et al., Cold Spring Harbor Laboratory, (1982) is a well recognized wor~ describing the various methods used to produce proteins in yeast.
For high level expression of a gene in yeast, it is essential to connect the gene to a strong promoter system as in the prokaryote and to also provide efficient transcription termination/polyadenylation sequences from a yeast gene. Examples of useful promoters include GALl,10, Johnston and Davis, Mol. and Cell. Biol., 4:1440-1448, 1984), ADH2, Russell, et al., J. Biol. Chem. 258:2674-2682, 1983), 20 PHO5, EMBOJ. 6:675-680, (1982), and MF~l. A multicopy plasmid with a selective marker such as Lue-2, URA-3, Trp-l, or His-3 is also deslrable. The MF~l promoter is preferred. The MF~l promoter, in a host of the ~ mating-type is constitutive, but is off in diploids or cells with the a mating-type. It can, however, be regulated by raising or lowering temperature in hosts which have a ts mutation at one of the SIR loci. The effect of such a mutation at 35C on an ~
type cell is to turn on the normally silent gene coding for the a mating-type. The expression of the silent a mating-type gene, in turn, turns off the MF~l promoter. Lowering the temperature of growth to 27C reverses the whole process, i.e., turns the a mating-type off and turns the MF~l on, Herskowitz and Oshima, The Molecuiar Biology of the Yeast Saccharomyces, Strathern, Jones, and Broach, eds., Cold Spring Harbor Lab., Cold Spring Harbor, NY, 181-209, (1982).
The polyadenylation sequences are provided by the 3'-end sequences of any of the highly expressed genes, like ADHl, MF~l, or TPI, Alber and Kawasaki, J. of Mol. and Appl. Genet. 1:419-434, (1982j.

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-lo- ~2~163 ~ number of yeast expression plasmids li~e YEp6, YEpl3, YEp24 can be used as vectors. A gene of interest can be fused to any of the promoters mentioned above, and then ligated to the plasmids for expression in various yeast hosts. These plasmids have been fully described in the literature, Botstein, et al., Gene, 8:17-24, (1979);
Broach, et al., Gene, 8:121-133, (1979).
Two procedures are used in transforming yeast cells. In one case, yeast cells are first converted into protoplasts using zymo-lyase, lyticase or glusulase, followed by addition of DNA and poly-ethylene glyc~l (PEG). The PEG-treated protoplasts are then regener-ated in a 3% agar medium under selective conditions. Details of this procedure are given in the papers by Beggs, Nature (London), 275:104-109 ~lS78); and Hinnen, et al., Proc. Natl. Acad. Sci. USA, 75:1929-1933 (1978). The second procedure does not involve removal of the cell wall. Instead the cells are treated with lithium-chloride or acetate and PEG and put on selective plates, Ito, et al., J. Bact., 153:163-168, (1983).
The cDNA can be ligated to various expression vectors for use in transforming host cell cultures. The vectors all contain gene sequences to initiate transcription and translation of the proteins that are compatible with the host cell to be transformed.
In addition, the vectors preferably contain a marker to provide a phenotypic trait for selection of transformed ~ost cells such as dihydroolate r0ductase or metallothionein. Additionally a replica-ting vector might contain a replicon.
Illustrative of cell cultures useful for the production of pro-teins are cells of insect or mammalian origin. Mammalian cell systems often will be in the form of monolayers of cells although mammalian cell suspensions may also be used. Illustrative examples of mammalian cell lines include VER0 and HeLa cells, Chinese hamster ovary (CH0) cell lines, WI38, BHK, COS-7 or MDCK cell lines.
As indicated above, the vector which is used to transform the host cell preferably contains gene sequences to initiate the tran-scription and translation of the protein's gene sequence. These sequences are referred to as expression control sequences. When the host cell is of mammalian or insect origin illustrative useful expression control sequences are obtained from the SV-40 promoter, Science, 222, 524-527 (1983), the CMV I.E. promoter, Proc. Natl.

-11- 132 ~
Acad. Sci. 81:65g-663 (1984), the metallothionein promoter, Nature, 296, 39-42, (1982) or the baculovirus polyhedrin promoter (insect cells), Virol., 131, 561-565 (1983). The plasmid or replicating or integrating DNA material containing the expression control sequences is cleaved using restriction enzymes and adjusted in size as neces-sary or desirable and ligated with cDNA coding for proteins by means well known i.n the art.
As with yeast when higher animal host cells are employed, poly-adenylation or transcription terminator sequences from known ma~mal-ian genes need to be incorporated into the vector. An example of aterminator sequence is the polyadenylation sequence from the bovine growth hormone gene.
Additionally gene sequences to control replication in the host cell may be incorporated into the vector such as those found in bovine papillomavirus type-vectors, Saveria-Campo, "Bovine papilloma-virus DNA: a eukaryotic cloning vector", DNA Cloning Vol. II--A
practical approach, Glover, ed., IRL Press, Arlington, Virginia 213-238 (1985).
The preferred expression vector useful for expressing proteins in Chinese hamster ovary (C~lO) cells is a shuttle vector pSVC0~7 which replicates in both CH0 and E. coli cells utilizing ampicillin resistance and dihydrofolate reductase genes as markers in ~. coli and CH0 cells respectively. Plasmid pSVCOW7 also provides the polyadenylation sequence from bovine growth hormone which is neces-sary for expression in CH0 cells. Plasmid pSVCOW7 is cleaved and aviral promoter and cDNAs inserted.
The preferred expression vector useful in forming recombinant baculovirus for expressing proteins in insect cells is pAc373, Smith, et al., Mol. Cell. Biol. 3:2156-2165 ~1933). The pla.sMid replicates in E. coli cells utilizing ampicillin resistance, and provides the eukaryotic promoter and polyadenylation signal from the baculovirus polyhedrin gene for expression of genes. Plasmid pAc373 is cleaved and a cDNA is inserted adjacent to the promoter. This new plasmid is cotransfected with baculovirus (Autograpa californica nuclear poly-hedrosis virus) DNA into insect cells by calcium phosphate precipita-tion. Recombinant baculovirus in which the pAc373 polyhedrin gene containing a cDNA has replaced the resident viral polyhedrin gene by homologous recombination is detected by dot blot hybridization using : , ' -12- ~323~63 32P-labeled cDNA as a probe, Summers and Smith, A ~anual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures, Te-~.as A
M University, College StatLon, T~, 29-30 (1986). Insect cells infected with recombinant baculovirus may also be differentiated by their occlusion-negative morphology since the insertion of the cD~JA
into the polyhedrin gene prevents the synthesis of this occlusion-forming protein.
The preferred expression vector used in conjunction with bovine papilloma virus (BPV) for expressing proteins is pT~79 (Plasmid pTWF9 was deposited in accordance with the Budapest Treaty. Plasmid pTFW9 is maintained in an E. coli host and has been deposited with the Northern Regional Research Center, Peoria, Illinois, USA on November 17, 19~6 and assigned Accession Number NRRL B-18141.) The plasmid replicates in E. coli utilizing ampicillin resistance, and provides the mouse metallothionein promoter and SV40 polyadenylation signal for expression of genes. Plasmid pTFU9 is cleaved and a cDNA
is inserted adJacent to the promoter. This new plasmid is then cleaved to allow insertion of BPV. The recombinant plasmid is trans-fected into animal cells by calcium phosphate precipitation and foci of transformed cells are selected.
The host cells are competent or rendered competent for trans-fection by various means. There are several well-known methods of introducing DNA into animal cells. These include: calcium phosphate precipitation, fusion of the recipient cells with bacterial proto-plasts containing the DNA, treatment of the recipient cells withliposomes containing the DNA, and microinjection of the DNA directly into the cells. The transfected cells are cultured by means well known in the art, Biochemical Methods in Cell Culture and Virology, Kuchler, Dowden, Hutchinson and Ross, Inc., (1977). Recombinant glycoproteins expressed in one of the above eukaryotic expression systems are isolated from cell suspensions created by disruption of the host cell system by well known mechanical or enzymatic means.
Proteins which are designed to be secreted from the cells are isolated from the media without disruption of the cells. For purification of glycoproteins it is helpful to first apply the cytoplasmic fraction to a lentil lectin column which will specifi-cally bind glycoproteins. The eluted glycoproteins are then applied to an affinity column containing antibody.

-13- ~ 3 A typical glycoprotein can be divided into three regions. ~t the amino terminal end is a hydrophobic region called the signal sequence. This sequence of amino acids signals the transport of the glycoprotein to the cell membrane. Following transport the signal sequence is removed by cleavage. Downstream from the signal sequence is the extracellular domain o~ the mature glycoprotein. This is the immunogenic portion of the glycoprotein since it is accessible to antibodies. At the carboxy terminal end of the glycoprotein is the hydrophobic anchor region which causes the glycoprotein to be retained in the cell membrane. The HRSV F is a typical glycoprotein in that it contains an amino terminal signal sequence and carboxy terminal anchor sequence, Collins, et al., PNAS 81:7683-7687, (1984).
However, the HRSV G glycoprotein is unusual since its amino terminal end acts as both a signal and anchor region, Wertz, et al., PNAS 82:
4075-4079, (1985).
~ glycoprotein may be designed to be secreted fro~ cells into the surrounding media. This is accomplished by causing the early termination of the glycoprotein before transcription of the anchor region, Lasky, et al., Biotechnology, 2:527-532 (1984). Early termination may be accomplished by inserting a universal transla-tional terminator oligonucleotide into an appropriate site in the gene's DNA. These oligonucleotides are com~ercially available.
Early termination may also be accomplished by altering the reading frame, thus generating a translational termination codon.
The chimeric glycoprotein described below consists of the signal and extracellular domains of HRSV F linked to the extracellular domain of HRSV G, and will be referred to as FG. The majority of the extracellular domain of the G glycoprotein is contained within the coding region spanned by the DdeI (nucleotide position 302) and FoKI
(nucleotide position 850) restriction enzyme sites. This sequence does not code for the signal/anchor region of the glycoprotein. The majority of the extracellular domain of the F glycoprotein is con-tained within the coding region prior to the NsiI (nucleotide posi-tion 1479) restriction enzyme site. This sequence codes for the signal region and the majority of the antigenic region, but not the anchor region of the glycoprotein.
To insert the G glycoprotein sequence into the F glycoprotein, the plasmid G-16 containing the HRSV gpG is digested with DdeI and .,, .,,,~. - , ' , .

-14- 132~1~3 FoKI and th0 ends are made blunt with Klenow polymerase. rne 550 bp fragment is then isolated by agarose gel electrophoresis. ~ne plasmid pGPF-4 containing the HRSV gpF gene was digested with NsiI.
The ends were made blunt with T4 DNA polymerase and dephosphorylated with bacterial alkaline phosphatase. The 550 bp fragment from G-16 is then ligated into the pGPF-4 plasmid and transformed into E. coli HB101. One of the clones, pGPFG-l, isolated from the transformation is verified as having the correct junctions by Maxim-Gilbert sequenc-ing.
When properly placed in a eukaryotic expression vector, the FG
gene described above is designed to express a chimeric glycoprotein which would be transported to the cell's surface and secreted into the media.
The above restriction enzyme sites were chosen because they allow for the expression of a large proportion of the relevant regions of the F and G glycoproteins. However, other portions of the glycoproteins could be expressed by choosing other restriction enzyme sites within the F and G coding sequences for the fusion of these genes. For instance, the restriction enzymes AluI, HincII or HinfI
could be used to cleave at the 5' end of the gpG gene. The restric-tion enzymes HphI, ~boII or XhoII could be used to cleave at the 3' end of the gpG gene. The enzymes could be used in any combination of two with one enzyme being from each group to give immunogenic protein fragments. For the gpF gene, the HinfIII, ~lincII, AvaII, SspI or HphI restriction enzymes could be used in place of NsiI. Linker oligonucleotides could be added to correct the reading frame in the junction regions. Two oligonucleotides which would correct the two possible frarne shifts are the SalI linkers GGTCGACC and CGGTCGACCG
CCAGCTGG GCCAGCTGGC
which are cornmercially available. Also when an anchor region is desired in the glycoprotein, a linker oligonucleoti~le is added at the second junction to allow synthesis of the gpF anchor region.
Alternative strategies could be designed for the expression of a FG
fusion protein by insertion or deletion of various sequences. The major criterion for the protein is the retention of a signal sequence and the immunologically important regions of the two glycoproteins.
Insertion oi` FG gene into CHO, BPV, or baculovirus expression - . . --15- ~ ~2~3 vectors is as already described.
The FG chimeric glycoprotein offers advantages over expression of the individual glycoproteins. Since FG is a single protein, it requires half the labor and reagents for purification compared to the separate F and G glycoproteins. Also, the FG chimeric glycoprotein is secreted into the media for ease of purification. The F glycopro-tein can be engineered as a secreted glycoprotein by truncation prior to the anchor region sequences. However, the HRSV G glycoprotein contains a signal/anchor region at its amino terminal end. There-iore, truncation of this glycoprotein will not generate a secretedform. The signal/anchor region could be replaced with a signal region from a foreign glycoprotein, but this would introduce foreign protein sequences into the potential vaccine.
The HRSV F and G glycoproteins have been expressed using a vaccinia virus expression system, and these recombinant viruses have been used as vaccines in the protection of cotton rats from HRSV
infection, Olmsted, et al., PNAS 83:7462-7466, (1986). Vaccinia virus expressing the F glycoprotein was significantly more immuno-genic and provided better protection than vaccinia virus expressing the G glycoprotein, Olmsted et al., supra. Vaccination with both viruses did not appear to have an additive effect over F alone Olmsted, et al., supra. In contrast, the secreted FG glycoprotein appears to be more immunogenic and provide better protection than a secreted form of the F glycoprotein. Also, vaccination of cotton rats with the FG protein produces a higher percentage of neutralizing antibody as defined by the ELISA to neutralization ratio.
An experiment was designed to compare the immunogenicity of FG
and truncated F (Ft). ~ecause the recombinant glycoproteins could not be detected in ConA (a lectin which binds glycoproteins) purified extracts by Commassie blue staining of proteins electrophoresed in SDS-PAGE gels (probably less than 1~ of the protein), an indirect method was used to determine equivalent amounts of the glycoproteins.
Densitometer tracings of autoradiograms containing 35S-methionine labeled protein which had been electrophoresed on a SDS-PAGE gel was used to determine the relative amount of FG and Ft in the samples (FG
and Ft contain the same number of methionines). These same samples were then assayed by ELISA, and it was determined that equivalent amounts of FG react 3 times better than Ft in our ELISA assay. The .
.

-16- 132~ 63 amount of FG or Ft in the samples prepared for vaccination was then determined by ELISA and equalized according to the above ratio. rne groups in the study were FG, Ft (high dose), Ft (low dose), and gp50 (neg. control). The cotton rats are vaccinated three times in Freund's adjuvant, 500 ~g total protein per dose. The amount of specific glycoprotein in the FG group is equivalent to the low dose Ft group. The high dose Ft group received 3 times more specific glycoprotein. A summary of the data from this study is presented below.
LUNG TITER ELISA TITER NEUT. TITER ELISA/NEUT.
GROUP (pfu/gm lung) (50% end pt)(50~ end pt) Ratio FG <55 1300 850 1.53 Ft high 2.0 x 102 1400 285 4.91 Ft low 6.1 x 103 1000 206 4.85 gp50 2.7 x 105 <100 40 ND

The above study demonstrates the efficacy of the chimeric FG
glycoprotein using crude preparations of FG in Freund's adjuvant. To demonstrate the efficacy of FG to induce high titers of neutralizing antibody and protect cotton rats from RSV challenge, a study was undertaken using more purified preparations of FG formulated in an adjuvant acceptable for human use (alum). FG was purified to 50%
homogeneity using a two step procedure involving cation exchange and monoclonal antibody affinity columns. The Ft glycoprotein was purified to 50~ homogeneity by lentil lectin chromatography followed by monoclonal antibody affinity chromatography. Cotton rats were vaccinated twice with these glycoproteins adsorbed in alum (2.5 mg alum/dose). One group of rats was vaccinated intranasally with live RSV as a positive control. One group of rats was vaccinated with the alum adjuvant as a negative control. Rats from each group were tested for serum and neutralizing antibody. The rats were challenged with RSV and the lungs were assayed for virus.
#Infected/ Avg. Lung Titer 35Group Serum Ab Neut. Ab Total of Infected Rats 25 ~g FG + Alum 19500 2834 0/7 ---5 ~g FG + Alum 19200 2027 0~6 ---1 ~g FG ~ Alum 12000 4096 3/73.5 X 102 ... :": ~

, " -17- ~32~163 25 ~g FG -~ CFA 21500 5029 1/7 1 0 X 10~
25 ~g FT + Alum 13500 263 2/7 3.0 X 102 5 ~g FT + Alum 13000 194 4/7 6.7 X 102 Alum Cont. <500 10 7/79.7 X 104 RSV I.N. 7100 217 1/7 50 -Conventions used to represent plasmids and fragments in Charts 1-6, are meant to be synonymous with conventional circular represen-tations of plasmids and their fragments. Unlike the circular fig-ures, the single line figures on the charts represent both circularand linear double-stranded DNA with initiation or transcription occurring from left to right (5' to 3'). Asterisks (*) represent the bridging of nucleotides to complete the circular form of the plas-mids. Fragments do not have asterlsk marks because they are linear pieces of double-stranded DNA. Endonuclease restriction sites are indicated above the line. Gene markers are indicated below the line. Bars appearing below the diagrams representir,g the plasmid or fragments are used to indicate the number of basepairs between two points on the DNA. The relative spacing between markers do not indicate actual distances but are only meant to indicate their rela-tive positions on the illustrated DNA sequence.
Examples Example 1 Removing the G-C taîls from the F glycoprotein gene In order to obtain maximum expression of the F glycoprotein, the G-C nucleotides which are used to insert the cDNA into the plasmid pBR322 must be removed from the 5' end (relative to the original mRNA) of the cDNA. In order to conveniently insert the gpFG cDNA
into the preferred expression vector for CH0 cells, pSVCOW7 (describ-ed below), it is necessary to supply a BamHI site upstream from the protein coding sequence. To accomplish this the cDNA of F glycopro-tein is inserted into pUC12 (PL Pharmacia Labs, Piscataway, NJ).
Methods for the synthesis of the cDNA cIone F5-25 containing the entire sequence for the F glycoprotein has been described. Collins, et al., ~ucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus, J. Virol., 81:7683-7687 (December 198~).
A. Construction of pGPF2 - Chart 1 The cDNA of the F glycoprotein is flanked by PstI sites (Chart -18- ~7,~ 6~
1), however there are also internal PstI sites. Therefore, the plasmid pF5-25 is partially digested with PstI and fragment 1 (1.9 kb) is isolated from a gel. Fragment 1 is li~ated to the plàsmid pUC12 (Bethesda Res. Labs,, Rockville, MD) which had been digested with PstI. A plasmid with the 5' end of the gpF gene adjacent to the XbaI site in pUC12 is selected and designated pGPF2 (4.6 kb). This orientation is verified by cleavage with. NsiI and HindIlI which ~enerates a fragment of approximately 400 bp.
B. Construction of pGPF3 and pGPF4 - Chart 2 To remove the G-C nucleotides from the 5' end of the cDNA, pGPF2 is opened with XbaI and the ends are treated with bacterial alkaline phosphatase to yield fragment 3. Fragment 3 is then digested with SalI which cuts off a small piece between the Xba-I and PstI sites and treated with Klenow enzyme to make the ends flush. After treatment with Klenow enzyme, fragment 3 i5 digested with Lambda exonuclease which requires a 5' phosphate and leaves a 3' overhang. Because of the removal of the 5' phosphate on the end upstream from the gpF, the exonuclease will digest downstream toward the gpF sequence. The exonuclease is allowed sufficient time to remove nucleotides beyond the G/C tail region into the leader sequence. A synthetic sequence containing the first 15 bases of the leader sequence ls hybridized to fragment 3 and the missing bases filled in with Klenow enzyme and the ends ligated with T4 ligase to yield pGPF3 (4.6 kb) which is trans-formed into E. coli and its sequence verified.
To remove the G-C nucleotides from the 3' end of the cDNA, pGPF3 is opened with HindIII and treated with the exonuclease Bal 31 for a time sufficient to digest through the G-C nucleotides. The ends are made blunt with Klenow enzyme and the cDNA clone is freed from the vector DNA by digestion with BamHI. The cDNA fragment is isolated from a gel and ligated to plasmid pUC12 which has been digested with BamHI and HincII (HincII is compatible with blunt ends) to yield pGPF4. The plasmid is transformed into E. coli and an appropriate clone which was sufficiently di~ested with Bal31 is identified by sequencing. Alternatively, the G-C nucleotides may be removed by digesting with a restriction enzyme which has a unique site upstream from the G-C nucleotides. For gpF such an en~yme whould be HaeIII.
Since HaeIII cleaves upstream from the F gene's normal translation "!, 1; termination signal, a universal translation termination oligonucleo-~,~ .S.,~

. .

~.~, ' O ~6~

tide (New England Biolabs) would be ligated onto the F cDNA after digestion with HaeIII. The DNA would then be digested with BamHI and treated as described above for generating pGPF-4.
Example 2 Construction of a HRSV Chimeric FG Glycoprotein Gene-Chart 3 A. Preparation of the HRSV G glycoprotein gene Clone G2B-16 containing the entire coding region for the HRSV G
glycoprotein has been described (Wertz, et al., Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein, PNAS, 82:4075-4079 (1985)), Clone G2B-16 containing the G glycoprotein cDNA is digested with DdeI
and FoKI and the ends are made blunt with E. coli DNA polymerase (Klenow fragment). The DNA is then electrophoresed in a 1.5~ agarose gel. The 550 bp fragment (fragment 4) containing the relevant region of the G gene is excised from the gel and the DNA is purified from the agarose.
B. Insertion of the G cDNA fragment into the HRS~ F glycopro-tein gene The plasmid pGPF4 (Chart 2) is digested with NsiI. The ends are made blunt with T4 DNA polymerase and then dephosphorylated with bacterial alkaline phosphatase. The 550 bp fragment of the G cDNA is then ligated into plasmid pGPF4 to yield the chimeric FG gene (pGPFG-1). The plasmid is transformed into E. coli HB101. Clones are isolated and selected Eor the correct orientation of the G cDNA
within the F gene by digestion with HinfI which will generate junction fragments of 875 bp and 186 bp. The incorrect orientation of the G fragment will yield junction fragments of 650 bp and 400 bp upon HinfI digestion. The junction regions of a properly orientated clone are then verified as correct by Ma~am-Gilbert sequencing.
The above example will generate a gene coding for a chimeric glycoprotein containing the signal and immunogenic region of the F
glycoprotein linked to the~ immunogenic region of the G glycoprotein.
Since the second junction (G to F) causes a frame shift and transla-tional termination, no anchor region will be present in the glycopro-tein.
Example 3 Using DNA Oligonucleotide Linkers to Adjust the Read-ing Frame of a HRSV Chimeric FG Glycoprotein - Chart 4 If restriction enzymes other than those presented in example 2 :

, . . .
.
. .
~:
' , -20- ~2~
are used for linking the F and G genes, a frame shift may occur at the first junction between F and G leading to early translational termination of the glycoprotein. This can be overcome by using oligonucleotide linkers which will restore the correct reading frame.
A. Preparation of the HRSV G elycoprotein gene Clone plasmid G2B-16 containing the G glycoprotein cDNA is digested with HphI and the ends are made blunt with T4 DNA polymer-ase. The SalI linker CGGTCGACCG
GCCAGCTGGC
(New England Biolabs) is ligated to the ends of the DNA. The D~A is digested with SalI and electrophoresed in a 1 5~ agarose gel. The 410 bp fragment (fragment 5) containing the relevant region of the G
gene is excised from the gel and the DNA is purified from the agarose.
B. Insertion of the G cDNA fragment into the HRSV F glycopro-tein gene The plasmid pGPF4 is digested with NsiI and the ends are made blunt with T~ DNA polymerase. The SalI linker indicated above is ligated to the ends of the pGPF4 DNA. The DNA is digested with SalI
and electrophoresed in a 1~ agarose gel. The 4.4 kb fragment is excised from the gel and the DNA is purified from the agarose. The

4.4 kb pGPF4 DNA fragment and the 410 bp G fragment are ligated together forming pGPFG-2 and transformed into E. coli HB101. Clones are isolated and selected for the correct orientation of the G cDNA
within the F gene by digestion with HinfI which will generate junc-tion fragments of 768 bp and 220 bp. The incorrect orientation of the G fragment will yield junction fragments of 555 bp and 430 bp upon ~linfI digestion. The junction regions of this clone are then verified as correct by Maxam-Gilbert sequencing.
The above example will generate a gene coding for a chimeric ~G
glycoprotein similar to that in Example 2. The chimeric glycoprotein generated in this example would contain less of the immunogenic regions of the G glycoprotein than the chimeric generated in Example 2. Chimeric glycoproteins containing other regions of the two glyco-proteins can be generated as described in Examples 2 and 3 using the enzymes listed in the "Detailed Description" section.
Example 4 Using D~A Oligonucleotides to Generate Genes Coding -21- ~2~163 for Chimeric FG Glycoproteins of Vario-~s Lengths-Chart 5 Genes coding for chimeric FG ~lycoproteins containing various regions of the F and G glycoproteins can be generated using a com^
bination of restriction en~ymes and oligonucleotides. This procedure allows the F and G glycoproteins to be linked at any desirable point on their amino acid backbone, permitting incorporation or removal of regions likely to contain epitopes which will be recogni~ed by the host immune system. Individual amino acids may also be changed if so desired. Oligonucleotides are synthesiæed corresponding to the DNA
sequence from the point of desired linkage to a convenient restric-tion enzyme site. The glycoprotein gene is digested with that restriction enzyme and the oligonucleotide is ligated to the gene at the restriction enzyme site ~o generate a DNA fragment of the desired length. The oligonucleotides are synthesized with ends compatible with the restriction enzyme sites for easy ligation.
A. Preparation of HRSV F glycoprotein gene The plasmid pGPF4 is digested with NsiI and ligated to either oligonucleotide 1 (cDNA nucleotides 1483-1519) or a mixture of oligonucleotides 1 and 2. Oligonucleotides 1 and 2 (cDNA nucleotides 1483-1564) would extend the glycoprotein F DNA incorporated into the chimeric gene to the DNA sequences just prior to the anchor-encoding region of the F glycoprotein. The DNA sequences in these 2 oligo-nucleotides may code for additional epitopes found on the F glycopro-tein. Parentheses surround the nucleotides on the 3' end of oligo-nucleotide 1 which would be included if it were to be the ter~inal oligonucleotide. The indicated nucleotides code for a HindIII
restriction enzyme site. If oligonucleotide 2 is also to be included, then the indicated nucleotides on oligonucleotide 1 are excluded to a'low ligation of the 3' end of oligonucleotide 1 with the 5' end of oligonucleotide 2. The 3' end of oligonucleotide 2 also contains a HindIII site.
Following ligation of the oligonucleotide(s), the DNA is ~digested with HindIII (HindIII sites in oligonucleotide a~ 3' end of the F; gene and in the polylinker region of p~C12 plasmid) and the plasmid is religated. The DNA is transformed into E. coli HB101 and : ~ a clone containing the oligonucleotide(s) linked to the F gene is ~isolated (pGPF5). The presence of the oli.gonucleotide(s) in the ' :

-22- 1~16~
clone can be verified by hybridization of the clone with 32P-labeled oligonucleotide(s).
B. Insertion of glycoprotein G cDNA into the F glycoprotein gene S Clone G2B-16 is digested with HinfI and XhoII. The 277 bp frag-ment representing the cDNA region from nucleotide position 377 to 654 is gel purified. Oligonucleotides representing adjoining regions of the G cDNA are then ligated to each end of the G fragment. The DNA
sequences in these oligonucleotides may code for additional epitopes found on the G glycoprotein. The individual oligonucleotides were designed to incorporate regions which may contain unique epitopes.
The oligonucleotide ligated to the 5' end of the G cDNA may consist of either oligonucleotide 3 (cD~A nucleotides 297-377) or oligonuc-leotide 3 linked to oligonucleotide 4 (cDNA nucleotides 213-377).
The oligonucleotide ligated to the 3' end of the G cDNA may consist of oligonucleotide 5 (cDNA nucleotides 654-714), oligonucleotides 5-6 (cDNA nucleotides 654-774), oligonucleotides 5-6-7 (cDNA nucleotides 654-843), or oligonucleotides 5-6-7-8 (cDNA nucleotides 654-912).
Parentheses enclose nucleotides which would be included only in the terminal oligonucleotide. For instance, the enclosed nucleotides would not be included on oligonucleotide 5 if oligonucleotide 6 were to be added. These enclosed nucleotides code for a HindIII site and in the case of oligonucleotides S, 6, 7, and 8 a translational term-ination codon. The enclosed nucleotides are not included when an additional oligonucleotide(s) is to be added in order to allow liga-tion between the compatible ends of the ollgonucleotides. For instance, the 5' end of oligonucleotide 3 is compatible with the 3' -end of oligonucleotide 4 when the nucleotides enclosed by parentheses are not included in oligonucleotide 3.
Following ligation of the oligonucleotides to the G cDNA
fragment, the DNA is digested with HindIII and the enlarged G cDNA
fragment (fragment 7) is gel purified. The new G cDNA fragment is then ligated to the F clone prepared in section A of this example (pGPF-5) which has been digested with HindIII. The DNA is trans-formed into E. coli HB101 and a clone containing the G gene in the correct orientation within the F gene is isolated ~pGPFG-3).
Orientation is determined by digestion with appropriate restriction enzymes. The newly synthesized regions of the chimeric gene are ' , -23- 1 3 ~ 3 verified correct by Maxam-Gilbert sequencing. The clone may then be placed in various expression vectors as described below, C. Oligonucleotides 1 ) TCAATATCTCAAGTCMCGAGMGATTMCCAGAGC ( CTAGCA AAGCTT) ACGTAGTTATAGAGTTCAGTTGCTCTTCTMTTGGTCTCG GATCGT(TTCGAA) 2 ) CTAGCATTTATTCGTAAATCCGATGMTTATTACATMTGTAAATGCTGGTAAATCCMGCTT
AMTAAGCATTTAGGCTACTTAATAATGTATTACATTTACGACCATTTAGGTTCGAA

10 3) (AAGCTT)CCTCAG CTTGGMTCAGTCCCTCTMTCCGTCTGAAATTACATCACAATCACCA
( TTCGAA GGAGTC ) GMCCTTAGTCAGGGAGATTAGGCAGACTTTAATGTAGTGTTAGTGGT

CCATACTAGCTTCAACAACACCAGG
GGTATGATCGAAGTTGTTGTGGTCCTCA
4) AAGCTTCACAAAGTCACACCMCAACTGCMTCATACAAGATGCAACAAGCCAGATCAAGA
TTCGAAGTGTTTCAGTGTGGTTGTTGACGTTAGTATGTTCTACGTTGTTCGGTCTAGTTCT

ACACMCCCCAACATACCTCACCCAGAAT
TGTGTTGGGGTTGTATGGAGTGGGTCTTAGGAGTC

5) GATCCCAAACCTCAAACCACTAAATCAMGGAAGTACCCACCACCMGCCCACA(GAAGAG
GGTTTGGAGTTTGGTGATTTAGTTTCCTTCATGGGTGGTGGTTCGGGTGT CTTCTC

2 5 TAGAAGCTT ) (ATCTTCGAA)

6) GAAGAGCCMCCATCMCACCACCAAAACAAACATCATAACTACACTACTCACCTCCAAC
GGTTGGTAGTTGTGGTGGTTTTGTTTGTAGTATTGATGTGATGAGTGGAGGTTG
(ACCACA(TAGAAGCTT) TGGTGT (ATCTTCGAA)

7 ) ACCACAGGAAATCCAGAACTCACAAGTCAMTGGMACCTTCCACTCMCTTCCTCCGAA
CCTTTAGGTCTTGAGTGTTCAGTTTACCTTTGGMGGTGAGTTGAAGGAGGCTT

GGCAATCCA(AGCCCT TAGAAGCTT) CCGTTAGGT TCGGGA(ATCTTCGAA~

2~1~3 ~) AGCCCTTCTCAAGTCTCTACAACATCCGAGTACCCATCACAACCTTCATCTCCACCC M CA
AGAGTTCAGAGATGTTGTAGGCTCATGGGTAGTGTTGGAAGTAGAGGTGGGTTGT

CACCACGCCAGTAGAAGCTT
GTGGTGCGGTCATCTTCGAA
Example 5 Construction of a ~IRSV Chimeric FG Glycoprotein Gene Containing an Anchor ~egion - Chart 6 Examples 2, 3, and 4 illustrate the synthesis of genes coding for chimeric FG glycoproteins which do not contain anchor regions and will therefore be secreted into the medium of expressing cells. A
gene coding for a chimeric FG glycoprotein containing an anchor region can be synthesized. The anchor region would cause the reten-tion of the chimeric glycoprotein in the cellular membranes in a manner similar to most viral glycoproteins. The anchor region may be on the carboxy-terminal end of the glycoprotein so that the immuno-genic regions of the chimeric molecule from both the F and G glyco-proteins would protrude into the extracellular fluid. The gene described below will code for a chimeric glycoprotein consisting of the extracellular region of HRSV F, the extracellular region of HRSV
G, and the anchor region of HRSV F in the above order from amino-terminus to carboxy-terminus.
A. Insertion of the G cDNA fragment into the HRSV F glycopro-tein gene The clone G2B-16 is digested with DdeI and FoKI. The following oligonucleotides are then ligated to the ends of the DNA fragment:
9) ATGCATCACC
TACGTAGTGGAGT

10) CAAGTCGATGCAT
AGCTACGTA
Following ligation, the DNA is digested with NsiI and the 550 bp fragment of the G cDNA (fragment 8) is gel purified. The 550 bp fragment is then ligated into NsiI digested pGPF4. The DNA is trans-formed into R. coli HB101. Clones are isolated and selected for thecorrect orientation as described in Example 2. The junction regions of a properly orientated clone are then verified correct by Maxam-Gilbert sequencing. This clone (pGPFG-4~ may be placed in various , -25- 1~20163 expression vectors as described below.
Example 6 Construction of a HRSV Chimeric GF Glycoprotein Gene A portion of the extracellular region of the HRSV F glycoprotein may be placed at the carboxy-terminal end of the G glycoprotein.
This chimeric glycoprotein would consist of the signal/anchor region from the amino-terminus of G, the majority of the extracellular region of G, and a portion of the extracellular region of F in the above order from amino-terminus to carboxy-terminus.
A. Preparation of the HRSV G glycoprotein gene - Chart 7 To prepare clone G2B-16 for expression, the G C tails used in cDNA cloning must be removed and compatible restriction enzyme sites placed on its ends. Clone G2B-16 is digested with NlaIlI and FoKI.
NlaIII cleaves at position 18 and FoKI at position 846 on the cDNA
gene sequence. The following oligonucleotides are then ligated to the cDNA fragment:
11) GATCCAAATGCAAACATG
GTTTACGTTT

12) C M GTCTCTCTACAG
AGAGAGATGTCAGCT
Oligonucleotide 11 will ligate to the NlaIII site and generate a BamHI restriction enzyme site on the 5' end of the cDNA fragment.
Oligonucleotide 12 will ligate to the FoKI site and generate a SaII
restriction enzyme site on the 3' end of the cDNA fragment. The DNA
is electrophoresed in a 1.5~ agarose gel. The 850 bp G cDNA fragment (fragment 9) is excised from the gel and the DNA is purified from the agarose. The G cDNA fragment is then ligated into pUC12 which has been digested with BamHI and SalI to yield pGPG-l. The plasmid is transformed into E. coli HB101 and plasmid DNA is isolated.
B. Insertion of an F cDNA fragment into the HRSV G glycopro-tein gene - Chart 8 The clolle F5-25 is digested with XhoII and NsiI. XholI cleaves at position 446 and NsiI at position 1483 on the F cDNA gene sequence. The following oligonucleotides are then ligated to the cDNA fragment.
13) TCGACGGTGGTG
GCCACCACCTAG

14) TCAATATCTTAG L 3 2 01 6 3 ACGTAGTTATAG M TCAGCT
51igonucleotide 13 will ligate to the XholI site and will ~enerate SalI restriction enzyme site on the 5' end of the cDNA fragment.
Oligonucleotide 14 will ligate to the NsiI site and will generate a SalI restriction enzyme site and a translational terrnination codon on the 3' end of the cDNA fragment. The DNA is then di~ested with SalI, and the 960 bp F cDNA fragment (fragment 10) is gel purified. The F
cDNA fragment is then ligated into pGPG-l which has been digested with SalI. The plasmid is transformed into E. coli HB101. Clones are isolated and selected for the correct orientation of the F cDNA
within the G gene by digestion with BamHI and NsiI which will gener-ate a 1.8 kb fragment. The incorrect orientation will generate a 850 bp fragment. The junction regions of a properly orientated clone are then verified correct by Maxam-Gilbert sequencin~. This clone (PGPGF-l) may be placed in various expression vectors as described below.
Example 7 Expression of the Chimeric FG Glycoprotein of HRSV in CH0 Cells A. Construction of pSVCOW7 The starting plasmid pSV2dhfr (available from the American Type Culture Collection or prepared according to the proced~re of S. Sub-ramani,et al., "Expression of the Mouse Dihydrofolate Reductase Com-plementary Deoxyribonucleic Acid in Simian Virus 40", Molecular and 25 Cellular Biology 2:854-864 (Sept. 1981) is digested with BamHI and EcoRI to yield a 5.0 kb fragment containing the ampicillin resistance gene, the SV40 origin, and the dhfr gene. The second portion of pSVCOW7 is obtained from plasmid p~GH2R2 which is digested with the same restriction endonucleases used to cleave pSV2dhfr to obtain a 2~1 kb fragment containing the 3' end of genomic bovine - growth hormone gene, i.e., BGH gDNA. Plasmid p~GH2R2 is publicly available from an E. coli HB101 host, deposited with the Northern Regional Research Laboratories in Peoria, Illinois (NRRL B-15154).
The 5,0 kb and 2.1 kb Eragments are ligated to yield pSVC0~7 (7,1 kb), B. Cons~ruction of pGPFG-IE-PA
The genes constructed in Examples 2-6 may be used for expression of a chimeric glycoprotein in CH0 cells. The plasmid pGPFG-l will be used in the following example. The other chimeric genes are treated -27- ~ 3 2 a 1 ~ 3 as described for pGPFG-l except when otherwise indicated. The assembly of pGPFG-IE-PA is accomplished in two steps. First the gpFG
cDNA from pGPFGl is inserted into pSVCOW7 yielding pGPFG-PA and then the immediate early promoter of cytomegalovirus is inserted to initiate transcription of the HRSY-like protsins yield ng pGPFG-IEPA.

STEP 1. Plasmid pSVCOW7 is cut with EcoRI and PvuII and fragment 11 (600 bp) containing the polyadenylation sequence of bovine growth hormone extending from the PvuII site in the 3' most exon of the BGH
gene, to the EcoRI site downstream from the 3' end is isolated. For a complete discussion of the BGH polyadenylation sequence see the following references: (1) European patent application 0112012, published on 27 June 1984 wherein the identification and charac-terization of BGH genomic DNA is disclosed; (2) Woychik, R.P. et al., "Requirement for the 3' Flanking Re~ion of the Bovine Growth Hormone Gene for Accurate Polyadenylation", Proc. Natl. Acad. Sci.
USA 81:3944-3948 (July 1984); and, D.R. Higgs, et al., Nature 306:398-400 (24 November 1983) and references cited therein disclos-ing that the nucleotide sequence M TAAA characterizes the poly-adenylation signal at a location 11 to 30 nucleotides upstream(towards the 5' end) from the 3' end of the BGH gene.
A second sample of pSVCOW7 is cut with Eco~I and BamHI to yield fragmcnt 12 (5.8 kb). Fragment 12 can be alternatively derived from the EcoRI/BamHI fragment from parent plasmid pSV2dhfr available from Bethesda Research Laboratories. Fragment 12 contains the origin of repli~ation from pBR322 and an ampicillin resistance gene expressed in E. coli which allows for the selection of the plasmid in E. coli.
The fragment also contains the mouse dihydrofolate reductase cDNA in a construction that allows expression in mammalian cells. Subramani, et al., Mol. Cell. Biol. 1:854-864 (1981).
Plasmid pGPFGl is cut with HindIII (pGPFG-3 is digested with HpaI), treated with Klenow enzyme and recut with BamHI to yield fragment 13 (2.2 kb) which is gel isolated. The BamHI site is just upstream from the cDNA coding for the 5' untranslated sequences of the FG mRNA, and the HindIlI site is in p~C12 vector a few bases pairs beyond the PstI site near the 3' end of the gpFG cDNA (HpaII
site in pGPFG-3 is 95 bp from 3' end of FG cDNA).
- ~ Fragments 11, 12 and 13 are ligated to form pGPFG-PA (8.6 kb) -28- 13~ 3 which is a replication vector capable of shuttling between E coli and CH0 cells. Plasmid pGPFG-PA is transformed into E coli.
STEP 2. In step 2, pGPFG-PA is converted into expression plasmid pGPFG-IE-PA by inserting the immediate early gene promoter from human cytomegalovirus (CMV I.E. promoter). The CMV I.E. promoter is obtained from the PstI digestion of the CMV genome. The restriction endonuclease cleavage maps of the region of the human cytomegalovirus (CMV) genome containing the major immediate early gene (CMV I.E.) have been described in detail Stinski, et al., J. Virol. 46:1-14, 1983; Stenberg, et al., J. Virol. 49:190-19g, 1984; and, Thomsen, et al., Proc. Natl. Acad. Sci. USA, 81:659-663, 1984.
The Stinski and Thomsen references describe a 2.0 kilobase PstI
fragmen~ which contains the promoter for the major immediate early gene. When this 2.0 kb PstI fragment is isolated and digested with Sau3AI, a 760 basepair fragment is obtained among the products. This 760 base pair fragment can be distinguished from the other products by its size and the presence of a SacI cleavage site and a BalI
cleavage si~e within the fragment. Because of its convenient identification, utilization of this Sau3AI fragment is the preferred method of use of the CMV I.E. promoter as described in the present specification.
Plasmid pGPFG-PA is cleaved with BamHI, and a Sau3AI fragment containing the CMV immediate early promoter is ligated into the compatible BamHI site. Plasmids containing the CMV promoter fragment in an orientation such that transcription from the promoter would synthesize an mRNA for an HRSV-like protein are identified by cleavage of the plasmids with Sacl. The resulting plasmid is desig-nated pGPYG-IE-PA having the CMV I . E. promoter at the 5'-end of the cDNA and the BGH polyadenylation signal on its 3'-end. The plasmid is maintained in E. coli until transfection into CH0 cells.
C. Transfection and Culturing of CH0 CeIls~
PLasmid pGPFG-IÉ-PA is transfected into Chinese hamster ovary (CH0~ cells deficient in dihydrofolate reductase(dhfr) using the calcium phosphate method for transfection of DNA into cells which is described in detail by Graham, et al., Introduction of Macromolecules into Viable Mammalian Cells, Alan R. Liss Inc., N.Y., 1980, pp. 3-25.
The cell line used is the mutant DXB-ll originally available from L.
Chasin, of Columbia University and completely described in Proc.

'' ':~ " ' -:' ' `' : ' ".'_j -29- ~3~63 Natl. Acad. Sci. USA 77:4216-4220 (1980). The above methods for transfection relies on the fact that cells which incorporate the transfected plasmids are no longer dhfr deficient and will grow in Dulbecco's modified Eagle's medium plus proline.
Ii the chimeric glycoprotein does not contain an anchor region, then supernatant from CH0 cells expressing secreted chimeric FG
protein is clarified by low speed centrifugation. The supernatant is applied to a conconavalin A (or lentil lectin) column. The glyco-protein is eluted after extensive washing with a linear gradient of ~-D-methylglucoside (0-0.5 M) in the above buffer. The eluted glycoprotein is dialyzed against PBS containing 0.1~ Triton X-100 and applied to an affinity column. The affinity column is composed of éither polyclonal or monoclonal antibodies directed against HRSV
linked to Sepharose 4B beads (Pharmacia, Piscataway, New Jersey) by known techniques. The column is washed in dialysis buffer and the HRSV FG glycoprotein is eluted with PBS containing O.lM glycine (pH
2.5) and 0.1~ Triton X-10~. The glycoprotein is dialyzed against saline and checked for purity by electrophoresis on a SDS-PAGE gel.
If the chimeric glycoprotein contains an anchor region, then the CHO cells expressing the glycoprotein are washed in phosphate buffered saline (PBS) and then lysed in PBS containing 1.0% Triton X-100 and 1.0~ sodium deoxycholate. After pelleting the nuclei, thecytoplasmic extract is applied to a concona~alin A column and purified as described above for secreted glycoproteins.
Example 8 The Expression of HRSV GPFG Using Bovine Papilloma Virus (BPV) A. The construction of a cloning vector containing a nontran-scribable expression cassette suitable for replication in E. coli The constructions of pTFW8 and pTFW9 offer a convenient starting material for expressing HRSV proteins using BPV. The transcription terminator of the deposited plasmid prevents the expression of HRSV
proteins and must be removed in a single step excision and ligation.
1. Construction of PTFW8 Plasmid pdBPV-MMTneo (342-12) described in Mol. and Cell Biol., Vol 3 (No. 11):2110-2115 (1983) and obtained from Peter Howley of the National Cancer Institute, Bethesda, Maryland, USA. Plasmid pdfiPV-~MT neo (362-12) consists of three parts: a complete BPV-l genome * tra~le mark ~30- ~3~16~
(]oo%) opened at the unique BamHI site; pML2 (a "poison-minus"
derivative of pBR322); and a transcriptional cassette composed of the murine metallothionein I gene promoter, the neomycin phosphotrans-ferase II gene of Tn5, and the simian virus 40 early-region trans-criptional processing signals. Plasmid pd~PV-MMT neo (342-12) is first digested with BamHI to remove the BPV sequences which were isolated and stored for later insertion. The remaining fragment i5 religated using T4 ligase to form pMMpro.nptII (6.7 kb). Removal of the BPV genome facilitates later genetic-manipulations by creating unique restriction sites in the remaining plasmid. Afte-r the recombinations are complete, the BPV genome is replaced.
Plasmid p~pro.nptII is digested with BglII and a synthetic DNA
fragment 14 containing unique restriction sites is inserted and ligated using T4 ligase to yield pTFW8 (6.7 kb). Plasmid pT~8 is identical to p~pro.nptII except for the insertion of unique restric-tion sites between the murine metallothionein I gene promoter and the neomycin resistance gene.
2. Construction of pTWF9 Plasmid pTWF9 contains the transcription terminator TI from phage lambda inserted between the metallothionein I gene promoter and the neomycin resistance gene. The transcription terminator can be obtained fro~ Donald Court of the National Cancer Institute in Bethesda, Maryland USA. The transcription terminator is supplied in pKG1800sib3 which is the same as pUS6 as described in Gene, 28:343-25 350 (1984), except that tI carries the sib3 mutation as described in Guarneros et al., PNAS, 79:238-242 (1982). During the normal infection process of phage lambda, t`he tI terminator functions in the inhibition of bacteriophage ~ int gene expression from PL and in the termination of int gene transcription originating from PI. The 30 terminator is excised from pKG1800sib3 using AluI and PvuI as fragment 15 (1.2 kb), which is gel isolated and XhoI linkers are placed on either end of the fragment. The linkers are available from New England Biolabs, Beverly, MA, U~A. The terminator fragment bounded by XhoI complementary ends is then inserted into pTWF8 which has been previously digested with XhoI. The fragments are then ].igated using T4 DNA ligase to yield pTWF9 (7.9 kb). Plasmid pTWF9 was desposted in accordance with the Budapest Treaty. Plasmid pTFW9 is maintained in an ~. coli host and has been deposited with the :'' '':'' ' ' ' :
- "' ' -31- ~3~163 Northern Regional Research Center, Peoria, Illinois, USA on November 17, 1986 and assigned Accession Number NR~L B-18141.
B. The construction of pTFW/GPFG
The genes constructed in Examples 2-6 may be used for expression of a chimeric glycoprotein using BPV. The plasmid pGPFG-l will be used in this e~ample. The other chimeric genes are treated as described for pGPFG-l except when otherwise indicated. To construct pTFW/GPFG, pGPFGl is digested with BamHI and HindIII (pGPFG-3 is digested with BamHI and HpaII). Its ends are made flush with Klenow enzyme and synthetic BglII lin~ers (New England Biolabs) are ligated to the ends of the clone. The DNA is digested with BglII and desig-nated fragment 16 (2.2 kb). Fragment 16 containing the gpFG gene (2.2 Kb) is then isolated from a gel. The purified fragment is ligated into pTFW9 which has been digested with BgllI to yield pTFW/GPFG (10.1 kb).
C. Conversion of pTFW/GPFG into a eukaryote expression vector Plasmid pTFW/GPFG is converted into a eukaryote expression vec-tor by reinserting the 100% complete BPV-l genome excised with BamHI
in step a., of Example 8A. Plasmid pTFW/GPFG is cut with BamHI and the BPV-l intact genome, a 7.9 kb fragment is inserted to yield pTFW/
GPFG/BPV* (18.0 kb) which is replicated in E. coli until production of glycoprotein FG by eukaryotic cells is desired.
D. Expression of gpFG in murine C127 cells Prior to transfection into murine C127 cells, pTFW/GPFG/BPV* is digested with XhoI to excise the TI terminator and religated with T4 DNA ligase. The resulting plasmid pTFW/GPFG/BPV (16.9 kb) will now direct the expression of high levels of gpFG which is secreted into the culture media. The Cl27 cells are available from the American Type Culture Collection and grown in Dulbecco's modified minimal essential media containing 10% fetal calf serum. The levels of gpFG
proteins in ~he media of the C127 cells are determined by Western blot experiments with anti-RSV antibody and 125I-labeled protein A.
HRSV gpFG is purified from the culture media or cells as described in Example 7.
Example 9 The Expression of HRSV GPFG Using Baculovirus Virus The following example relates to the expression of glycoprotein FG in insect cell cultures. All procedures are detailed in Summers, M.D. and Smith, G.E., A Manual for Baculovirus Vectors and Insect -32- ~32~1~3 Cell Culture Procedures published by the College of Agriculture, Texas Agricultural Experiment Station, Texas Agricultural Extension Service, College Station, Texas, 1986. The starting plasmid pAc373 (7.1 kb) is a general baculovirus expression vector having a unique BamHI site immediately downstream from the polyhedron promoter for Autographa californica nuclear polyhedrosis virus (AcNPV). The polyhedron protein ls a matrix protein that is nonessential for viral infection and replication in vitro. The p]asmid is available from Professor Max Summers of the Department of Entomology, Texas A & M
Univarsity, College Station, Texas 77843 and is fully described in Molecular and Cell. Biology, 3(12):2156-2165 (1983).
A. Construction of pAcGPFG
The genes constructed in Examples 2-6 may be used for expression of a chimeric glycoprotein using baculovirus. The plasmid pGPFG-l will be used in this example. The other chimeric genes are treated as described for pGPFG-l except when otherwise indicated. Plasmid pGPFGl is digested with HindIII (pGPFG-3 is digested with HpaII) and the ends are made flush with Klenow enzynme. Synthetic BamHI linkers (New England Biolabs) are ligated to the end of the DNA. The DNA is digested with BamHI and fragment 17 (2.2 kb) containing the gpFG gene is isolated from a gel. The purified fragment is ligated into pAc373 which has been digested with BamHI.
B. Transfection and culturing of S. Frugiperda The gpFG cDNA insert of pAcGPFG is recombined with native AcNPV
DN~ by cotransfection in S. frugiperda. S. Frugiperda (SF9; ATCC CRL
1711) are cultured in Grace Media (Gibco Lab. Livonia, MI 48150), 10% fetal calf serum and supplemented with Difco Lactalbumin hydroly-solate and yeastolate. The cells are cotransfected with AcNPV DNA
and pAcGPFG at l~g/ml and 2~g/ml respectively. Resulting virus particles are obtained by collecting the media and removing cellular material by low speed centrifugation. The virus containing-media is then used to infect S. frugiperda. Subsequent infection of S.
frugiperda using these viral particles which include both native viral DNA and DNA recombined with the cDNA coding for glycoprotein FG
will result in some cells expressing the HRSV protein instead of the polyhedron protein. Purification of recombinant virus is accomp-lished by a series of limited dilution platings in 96-well tissue culture plates containing S. frugiperda cells. Wells containing ,,~.., ,.., .

`` 33 132~3 recombinant vinls are detected by dot blot hybridization using pGPFGl which has been labeled with 32p-dCTP by nick translation as a probe, Once sufficiently pure, the recombinant virus is detected by its unique occlusion-negative plaque morphology. HRSV protein synthe-sized in recombinant baculovirus infected cells is detected byWestern blot experiments with anti-RSV antibody and 125I-labeled protein A (Amersham Corp,).
The HRSV protein is purified from the culture media or cells as described in Example 7.
Example 10 The Construction of pAcGPFG Containing a Natural Poly-hedron Leader Seguence The plasmid pAc373 described in Example 8 contains a BamHI
linker sequence at the -8 position of the polyhedron leader to allow easy insertion of foreign genes. However, this disruption of the polyhedron leader sequence may result in lower levels of expression of the inserted gene than would be possible with the natural polyhed-ron leader. Described belo~ is a method for linking the natural polyhedron leader sequence to the initiation codon of the HRSV FG
gene. The genes constructed in Examples 2-6 may be used in this example for expression of a chimeric glycoprotein. The plasmid pGPFG-l will be used in this example. The other chimeric genes are treated as described for pGPFG-l.
A. Preparation of pAcGPFG-2 Plasmid pAcGPFG (Example 8) is digested with EcoRV and PstI.
EcoRV cleaves the polyhedron leade.r sequence at position -93 while PstI cleaves the HRSV FG coding sequence at positions +50, +636, and +1701 in the FG coding sequence, and in the pUC12 polylinker region adjacent to the 3' end of the FG gene. The DNA is electrophoresed in a 1% agarose gel and the large fragment (9.8 kb) containing primarily the plasmid pAc373 is purified from the gel.
An oligonucleotide consisting of the polyhedron leader sequence from positions -93 (EcoRV cleavage site) to -1 linked to the FG gene sequence from positions O (nucleotide A of the initiation codon~ to -~50 (PstI cleavage site) is synthesized and constructed. Because of the length of this sequence, the DNA is synthesized as several oligo-nucleotides which are then ligated together. The intact oligonucleo-tide is ligated to the 9.8 kb fragment prepared above. The DNA is transformed into E. coli HB101. Clones containing the new plasmid , ,., . - ~

-34- 13~16~
(pAcGPFG-2) are isolated and the newly synthesized region is verificd as correct by Maxam-Gilbert sequencing.
B. Inserting the FG gene into pAcGPFG-2 Plasmid pGPFG-l (Example 2) is partially digested ~ith PstI.
PstI cleaves at positions +50, +636, and +1701 in the FG coding sequence, and in the pUC12 polylinker region adjacent to the 3' end of the FG gene. The DNA is electrophoresed in a 1.2~ agarose gel The 2.2 kb fragment corresponding to the nearly intact FG gene (FG
position +50 to PstI site in pUC12 polylinker) is purified from the gel. The 2.2 kb fragment is then ligated into plasmid pAcGP~G-2 which had be~n digested with PstI. The DNA i5 transformed into ~.
coli HB101. Clones are isolated and checked for the correct orienta-tion of the FG gene by digestion with EcoRV and SspI which will generate a 2.3 kb fragment. The incorrect orientation will generate a 130 bp fragment. The above gene is inserted into the baculovirus genome for expression of the HRSV chimeric FG glycoprotein as described in Example 8.
Example ll Preparation of a Vaccine The immunogen can be prepared in vaccine dose form by well-known procedures. The vaccine can be administered intramuscularly, subcu-taneously or intranasally. For parenteral administration, such as intramuscular injection, the i~munogen may be combined with a suit-able carrier, for example, it may be administered in water, saline or buffered vehicles with or without various adjuvants or immunomodulat-ing agents such as aluminum hydroxide, aluminum phosphate, aluminu~potassium sulfate (alum), beryllium sulfate, silica, kaolin, carbon, water-in-oil emulsions, oil-in-water emulsions, muramyl dipeptide, bacterial endotoxin, lipid X, Corynebacterium parvum (Propionobacter-ium acnes), Bordetella pertussis, polyribonucleotides, sodium algin-ate, lanolin, lysolecithin, vitamin A, saponin, liposomes, levamis-ole, DEAE-dextran, blocked copolymers or other synthetic adjuvants.
Such adjuvants are available commercially from various sources, for example, ~erck Adjuvant 65 (Merck and Company, Inc., Rahway, NJ~.
The proportion of immunogen and adjuvant can be varied over a broad range so lon~ as both are present in effective amounts. For example, aluminum hydroxide can be present in an amount of about 0.5 of the vaccine mixture (A1203 basis~. On a per dose basis, the con-centration of the immunogen can range from about O.Ql5 ~g to about .

, ~,.. .

~35~ 132~16~
1.5 mg per kilogram per patient body weight. A preferable dosage range is from about 1.5 ~g/kg to about 0.15 mg/kg of patient body weight. A suitable dose size in humans is about 0.1 - 1 ml, prefera-bly about 0.1 ml. Accordingly, a dose for intramuscular injection, for example, would comprise 0.1 ml containing immunogen in admixture with 0.5% aluminum hydroxide.
The vaccine can be administered to pregnant women or to women of child bearing age to stimulate maternal antibodies. The female can be revaccinated as needcd. Infants can be vaccinated at 2 to 3 months of age after depletion of maternal antibodies and revaccinated as necessary, preferably at 6 to 9 months of age after maturation of the immune system. Babies born to unvaccinated mothers can be vaccinated at 2 to 3 months of age. The vaccine may also be useful in other susceptible populations such as elderly or infirmed patients.
~ he vaccine may also be combined with other vaccines for other diseases to produce multivalent vaccines. It may also be combined with other medicaments such as antibiotics.

-36- 1~2~

CONSTRUCTION OF pGPF2 (a) Plasmid pF5-25 is cut with PstI and fragment 1 (1.9 kb) is gel isolated.
Fragment 1 PstI PstI
l .. _ .
TTTFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFTTT

(b) Plasmid p~C12 (2.7 kb) is cut with PstI to yield fragment 2 10 which is gel isolated.
Fragment 2 PstI HindIII BamHI XbaI SalI Pstl I

AmpR
(c) Fragments 1 and 2 are ligated to yield pGPF2 (4.6 kb) ~Jhich is transfoxmed into E. coli.
BamHI XbaI SalI PstI PstI HindIII
* I . ~ _ I I *
¦ TTFFFFFFFTT
AmpR

AmpR ~ Ampicillin resistance T ~ Guanosine/cytosine tail F = Glycoprotein F

:
::

; :: ~ :
:~

', ~.

-37~ 1~20~3 CONSTRUCTION OF pGPF3 AND pGPF4 (a) Plasmid pGPF2 is cut with XbaI, treated with bacterial alkaline phosphatase, recut with SalI and treated with Klenow enzyme to yield frag~ent 3.
Fragment 3 SalI PstI Pstl Hindlll BamHI Xoal TTTFFFFFFFTTT
AmpR
(b) Fragment 3 is digested downstream from the SalI site using lambda exonuclease and the remaining 3' tail is hybridized to the synthetic oligonucleotide complementary to the 5' portion of the leader sequence having the following sequence of GpF cD~A.
5'-end AAATAACAATGGAG

(c) The single stranded portion of the cDNA 3' downstream from the synthetic oligonucleotides are filled in using Klenow enzyme and the ends are ligated using T4 ligase to yield pGPF3 (4.6 kb).
BamHI PstI HindIII
* ._. I I *
FFFFFFFFFFFFFFFFFFTTT
AmpR

(d) Plasmid pGPF3 is cut with HindIII and treated with Bal 31 to digest the G-C nucleotide tail at the 3' end of the gpF CDNA. The gpF cDNA is cut with BamHI (1.7 kb) isolated from a gel and religated into a BamHI/HincII digestion of PUCl2 to yield pGPF4 (4.4 kb).
BamHI HindIII
* I I - *
FFFFFFFF
~ AmpR

: AmpR = Ampicillin resistance 35 T = Guanosine/cytosine tail F = Glycoprotein P

.
:

: ' ' .

' -38- ~32~ ~3 CONSTRUCTION OF A CHI~ERIC FG GLYCOPROTEIN GENE
Plasmid G2B-16 PstI DdeI FoKI PstI
5 * ~ ~ *
TTTGGGGGGGGGGGTTT
TcR
(a) Plasmid G2B-16 is digested with DdeI and FoKI, and the ends are made blunt with Klenow enzyme. The DNA is electrophoresed in a 1.5~ agarose gel and fragment 4 (550 bp~ is purified from the agarose.
Fragment 4 GGGGGGGGGGG

(b) Plasmid pGPF-4 (Chart 2) is digested with NsiI. Tha ends are made blunt with T4 DNA polymerase and dephosphorylated with bacterial alkaline phosphatase. Fragment 4 is then ligated into the plasmid to form pGPFG-l (5.0 kb).
Plasmid GPFG-l BamHI HindIII

FFFFFFFFFFGGGGGGG FFFF
¦ AmpR
Term AmpR Ampicillin resistance TcR Tetracycline resistance T - Guanosine/Cytosine tail G ~ DNA seq~ences for G glycoprotein F ~ = DNA sequences for F glycoprotein Term ~ TranslationaI termination signal .
:

, .. . . ..
---.

-USING LINKERS TO ADJ~ST THE READING FRAME OF FG
Plasmid G2B-16 PstI HphI ~IphI PstI
5 * I I I I *
TTTGGGGGGGGGGGGGTTT
TcR
SalI I,inker CGGTCGACCG
GCCAGCTGGC
10 (a) Plasmid G2B-16 is digested with HphI and the ends are made blunt with T4 DNA polymerase. The SalI linker is ligated to the ends of the cDNA. The DNA is digested with SalI and fragment 5 (410 bp) is gel purified.
Fragment 5 ~
LGGGGGGGGGGGGGGGGGGL

(b) Plasmid GPF4 ~Chart 2~ is digested with NsiI and the ends are made blunt with T4 DNA polymerase. The SalI linker is ligated to the ends of the cDNA. The DNA is digested with SalI and the plasmid (4.4 kb) is gel purified. Fragment 5 is then ligated to the gel purified GPF4 to form pGPFG-2.
Plasmid GPFG-2 BamHI HindIlI
* I I *

¦ ArnpR
Term AmpR = Ampicillin resistance TcR ~ Tetracycline resistance T ~ Guanidine/Cytosine tail F = DNA sequences for F glycoprotein G = DNA sequences for G glycoprotein L = SalI linker Terrn = Translational Termination Signal :
~., ~2016~

USING OLIGONUCLEOTIDES TO GENERATE FG GENES OF VARIOUS LENGTHS
(a) Oligonucleotide A consists of oligonucleotide 1 (36 bp) or oligonucleotides 1 and 2 ligated together (81 bp). Oligonucleotide B
consists of oligonucleotide 3 (80 bp) or oligonucleotides 3 and 4 ligated together (164 bp). Oligonucleotide C consists of oligonucle-otide 5 (60 bp), or oligonucleotide 5 and 6 ligated together (120 bp) or oligonucleotides 5, 6, and 7 ligated together (189 bp), or oligo-nucleotides 5, 6, 7, and 8 ligated together (258 bp). Oligonucleo-tides A, B, and C are gel purified.
Oligonucleotide A Oligonucleotide B Oligonucleotide C

lllllll 333333 5555 (b) Plasmid GPF-4 is digested with NsiI and oligonucleotide A
is ligated into the NsiI site. The DNA is digested with HindIII and the plasmid is religated to form pGPF-5.
Plasmid GPF-5 BamHI NsiI HindIII
* ~ .-.---.. ----I i , *
FFFFFFFFFFAAAA
AmpR

(c~ Plasmid G2B-16 is digested with HinfI and XhoII, and fragment 6 (277 bp) is gel purified.
Fragment 6 HinfI XhoII

GGGGGGGGGGG

.. .

-41- ~ 3 2 0 1 6 3 CHART S (continued) USING OLIGON~CLEOTIDES TO GENERATE FG GENES OF VARIO~S LENGTHS
(d~ Oligonucleotides B and C are ligated to fragment 6. The DNA is digested with HindIII and fragment 7 is gel purified (length of fragment 7 varies from 417 bp to 700 bp depending on oligonucleo-tides contained within oligonucleotides B and C).
Fragment 7 HindlII HinfI XhoII HindIII

BBBBGGGGGCCCCC
(e) Plasmid GPF-5 is digested with HindIII and dephosphorylated with bacterial alkaline phosphatase. Fragment 7 is then ligated into the HindIII site of pGPF-5 to form pGPFG-3.
Plasmid GPFG-3 HindIII HindIII HindIII HpaII
15 *I - L I I *
FFFFFFFFAAABBGGGGCC
¦ AmpR
Term AmpR = Ampicillin resistance F ~ DNA sequences for F glycoprotein G - DNA sequences for G glycoprotein A = Oligonucleotide A
B = Oligonucleotide B
C = Oligonucleotide C
le~ - Tr.nslatLonal Term~nation ~ignal .

.

-4~~ 1 3 2 ~ 1 ~ 3 CONSTRUCTION OF AN FG GENE CONTAINING AN ANCHOR REGION
Plasmid G2B-16 PstI DdeI FoKI PstI
5 * I I I I *
TTTGGGGGGGGGGGGTTT
TcR
(a) Plasmid G2B-16 is digested with DdeI and FoKI. Oligon~cleo-tides 9 and 10 are ligated to the ends of the DNA. The DNA is digested with NsiI and fragment 8 (550 bp) is gel purified.
Fragment 8 NsiI NsiI

(b) Plasmid GPF-4 is digested with NsiI and fragment 8 is ligated into the NsiI site to form pGPFG-4.
Plasmid GPFG-4 BamHI HindIII
* ~ . *

¦ AmpR
Term AmpR = Ampicillin resistance TcR ~ Tetracycline resistance T = Guanosine/Cytosine tail F ~ DNA sequences for F glycoprotein G = DNA sequences for G glycoprotein 9 = Oligonucleotide 9 = Oligonucleotide lO
Al ;=-DNA sequences coding for ~anchor region of F glycoprotein Term = Translational Termination Signal :: :

~ ~ :

:: ~

43 ~ 3 PREPARATION OF G GENE FOR CONSTRUCTION OF GF CHIME~IC GEN~
Plasmid G2B-16 PstI NlaIII FoKI PstI
5 * I I L L _ *
TTTGGGGGGGGGGGGGTTT
TcR
(a) Plasmid G2B-16 is digested with NlaIII and FoKI. Oligonuc-leotides 11 and 12 are ligated to the ends of the DNA and fragment 9 (850 bp) is gel purified.
Fragment 9 BamHI SalI
_l llGGGGGGGGGG12 (b~ Plasmid pUC12 is digested with BamHI and Sall, and dephos-phorylated with bacterial alkaline phosphatase. Fragment 9 is ligated into the plasmid to form pGPG-l.
Plasmid GPG-l BamHI SalI
20 * -------- I I *
llGGGGGGGGGGGGG12 AmpR
AmpR ~ Ampicillin resistance TcR -~ Tetracycline resistance T 8 Guanosine/Cytosine tail G ~ DNA sequences for G glycoprotein 11 = Oligonucleotide 11 12 = Oligonucleoti.de 12 :

:

.
~ . ''`: - . , ,~
;

.

44 ~32~

INSERTION OF G cDNA INTO pGPG-l Plasmid F5-25 PstI XhoII NsiI PstI
5 * ~ *
TTTFFFFFFFFFFFTTT
(a) Plasmid F5-25 is digested with XhoII and NsiI. Oligonucle-otides 13 and 14 are ligated to the ends o the DNA. The DNA is digested with SalI and fragment 10 (960 bp) is gel purified.
Fragment 10 SalI SalI

I

Term (b) PGPF-l is digested with SalI and dephosphorylated with bac-terial alkaline phosphatase. Fragment 10 is then ligated into the plasmid to form pGPGF-l.
Plasmid GPGF-l BamHI SalI SalI
* ~L I *
llGGGGGGGGGG12 13FFFFF14 ¦ AmpR
Term AmpR = Ampicillin resistance TcR ~ Tetracycline resistance T = Guanosine/Cytosine tail G = DNA sequences coding for G glycoprotein F = DNA sequences coding or F glycoprotein 11 = Oligonucleotide 11 12 = Oligonucleotide 12 13 = Oligonucleotide 13 14 = Oligonucleotide 14 Term = Translational Termination Signal ~320~3 GLYCOPROTEIN FG

1 Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 5 17 Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 33 Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 49 Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu 7 eu S~r Asn Ile 65 Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys 10 81 Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 97 Met Gln Ser Thr Pro Pro Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 113 Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 129 Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Gly 145 Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser l.ys Val Leu His Leu 161 Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 177 Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 193 Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 209 Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 20 225 Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 241 Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 257 Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 273 Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 25 289 Met Ser Ile Ile Lys G].u Glu Val Leu Ala Tyr Val Val Gln Leu Pro 305 Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 321 Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 337 Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 30 353 Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 369 Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Ile Asn Leu Cys Asn Val 385 Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 401 Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 35 417 Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 433 Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Met Asp 449 Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 465 Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn ~Phe Tyr Asp Pro ~ -46~ 163 CHART 9 (continued) GLYCOPROTEIN FG

481 Leu Val Phe Pro Ser Asp Glu Phe Asp Gln Leu Gly Ile Ser Pro Ser 497 Asn Pro Ser Glu Ile Thr Ser Gln Ile Thr Thr Ile Leu Ala Ser Thr 513 Thr Pro Gly Val Lys Ser Thr Leu Gln Ser Thr l'hr Val Lys Thr Lys 529 Asn Thr Thr Thr Thr Gln Thr 51n Pro Ser Lys Pro Thr Thr Lys Gln 545 Arg Gln Asn Lys Pro Pro Ser Lys Pro Asn Asn Asp Phe His Phe Glu 561 Val Phe Asn Phe Val Pro Cys Ser Ile Cys Ser Asn Asn Pro Thr Cys 577 Trp Ala Ile Cys Lys Arg Ile Pro Asn Lys Lys Pro Gly Lys Lys Thr 593 Thr Thr I.ys Pro Thr Lys Lys Pro Thr Leu Lys Thr Thr Lys Lys Asp 609 Pro Lys Pro Gln Thr Thr Lys Ser Lys Glu Val Pro Thr Thr Lys Pro 625 Thr Glu Glu Pro Thr Ile Asn Thr Thr Lys Thr Asn Ile Ile Thr Thr 641 Leu Leu Thr Ser Asn Thr Thr Gly Asn Pro Glu Leu Thr Ser Gln Met 657 Glu Thr Phe His Ser Thr Ser Ser Glu Gly Asn Pro Ser Pro Ser Gln 673 Val Asn Ile Ser Ser Gln Arg Glu Asp

Claims (20)

1. A polypeptide comprising a signal sequence and at least one immunogenic fragment from both human respiratory syncytial virus glycoproteins F and G.

2. A polypeptide according to claim 1 wherein said polypeptide is, beginning with the N terminal end, the signal sequence from glycopro-tein F, an immunogenic fragment of glycoprotein F and an immunogenic fragment of glycoprotein G.

3. A polypeptide according to claim 1 which is

4. A human vaccine comprising a polypeptide of claim 1.

5. A vaccine according to claim 4 wherein said polypeptide is, beginning with the N terminal end, the signal sequence from glycopro-tein F, an immunogenic fragment of glycoprotein F and an immunogenic fragment of glycoprotein G.

6. Use of the vaccine of claim 4 to prepare a medicament for protecting humans from human respiratory syncytial virus.

7. A use according to claim 6 wherein said polypeptide is, beginning with the N terminal end, the signal sequence from glycoprotein F, an immunogenic fragment of glycoprotein F and an immunogenic fragment of glycoprotein G.

3. An expression system comprising a suitable host containing a DNA
sequence capable of expressing a polypeptide of claim 1.

9. The expression system according to claim 8 wherein said DNA
sequence produces a polypeptide which is, beginning with the N
terminal end, the signal sequence from glycoprotein F, an immunogenic fragment of glycoprotein F and an immunogenic fragment of glycopro-tein G.

10. The expression system according to claim 8 wherein said suitable host is selected from group consisting of bacteria cells, yeast cells, mammalian cells and insect cells.

11. The expression system according to claim 10 wherein said suitable host is selected from the group consisting of E. coli cells, Chinese hamster ovary cells, murine C127 cells and S. Frugipersa cells.

12. The expression system according to claim 8 wherein said suitable host secretes said polypeptide.

13. The expression system according to claim 8 wherein said DNA
sequence is contained in a plasmid.

14. The expression system according to claim 13 wherein said plasmid is under the control of a cytomegalovirus promoter.

15. The expression system according to claim 13 wherein the replica-tion of said plasmid while in a suitable eukaryote host is under the control of bovine papilloma virus DNA sequences.

16. The expression system according to claim 8 wherein said DNA
sequence is contained in a recombinant virus of the baculovirus family.

17. The expression system according to claim 16 wherein the virus is Autographa californica nuclear polyhedral virus.

18. A polypeptide comprising at least one immunogenic fragment from both human respiratory syncytial virus glycoproteins F and G.

19. A human vaccine comprising a polypeptide of claim 18.

20. Use of a vaccine according to claim 19 to prepare a medicament for protecting humans from human respiratory syncytial virus.